LOCUS       Exported                4549 bp ds-DNA     circular UNA 16-NOV-2016
DEFINITION  natural circular DNA
ACCESSION   BGHA
VERSION     .
KEYWORDS    73272_Genbank
SOURCE      Saccharomyces cerevisiae
  ORGANISM  Saccharomyces cerevisiae
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Mathew Jessop Fabre
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 16, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
     gene            complement(1..859)
     CDS             complement(1..859)
                     /codon_start=1
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1219..1238
                     /note="BGHA F1"
     misc_feature    1304..1310
                     /note="Nt.BvcI"
     misc_feature    1318..1889
                     /note="X-3 UP"
     misc_feature    1897..1903
                     /note="Nt.BvCI"
     misc_binding    1903..1914
                     /note="MCS 1"
                     /note="Oriinal location: 1329..1371"
     terminator      1928..2122
                     /note="T ADH1"
     terminator      2152..2354
                     /note="T CYC1"
     misc_feature    2450..2456
                     /note="Nt.BvcI"
     misc_feature    2464..3004
                     /note="X-3 Down"
     misc_feature    3012..3018
                     /note="Nt.BvcI"
     rep_origin      complement(3804..4447)
                     /direction=LEFT
                     /note="pUCori"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcga
     1321 gatctttgtg ttcggttacc cggctcagat cctaacttct tcttttggta tgtttattcg
     1381 tataagttac tgttgtccac aggcaatact ctgcagaaaa ttaaaacggc attaatgcta
     1441 ggacaaccag aattgttact actgtatgtg cgatagttga taactgcaac attatgcccg
     1501 gtatattctc aaaaaaccct attactgcat acgaagaaat cgcaagagaa atctttcggt
     1561 ttggaaaagc tcactgtgag gttccttgga gccaatagta atacagcaca atccaaggaa
     1621 aaatctggcc tatatgcaag gaaggagaga tagtcaaaag cattctttcc cctagaagtt
     1681 ggtgcatata tggcatcgtt aaaacatatt acccccaaaa tttcttctct aaacgatgtg
     1741 cttggccttt gttttggttt ttgatgtcgg tcgtttgagg ccccttgcgg aaaatcgaga
     1801 tcgccgaatg gcacgcgagg gaagggaaat aaggtttaaa ggcactgaaa caataggcaa
     1861 gaagtaggcg agagccgaca tacgagacta ttaagtcctc agcgagctcg catggaatgc
     1921 gtgcgatgag cgacctcatg ctatacctga gaaagcaacc tgacctacag gaaagagtta
     1981 ctcaagaata agaattttcg ttttaaaacc taagagtcac tttaaaattt gtatacactt
     2041 atttttttta taacttattt aataataaaa atcataaatc ataagaaatt cgcttattta
     2101 gaagtgtcaa caacgtatct accaacggaa tgcgtgcgat cgcgtgcatt catccgctct
     2161 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt
     2221 tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg
     2281 cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa
     2341 gatcgcgacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     2401 tacaacgtcg tgactgggaa aaccctggcg ttacccctgc aggactagtg ctgaggcatt
     2461 aatccacttt tcaatgaaac ggatattgat atgctagtaa aaggacgagc tcaagagcga
     2521 aaatataagt aaagaattcg agtgcacttg tctccatgca gcaagatttc atatgagtct
     2581 tttttatctt tttacttttt acattacacg atatgcactt tatgaaaatt taacgaggtt
     2641 ggaagccgga taatcaacca aaatcaggca cgaaggcaca ctcgtatatg catgttgttg
     2701 aaactctgtt acgctgaact aacaatcaca catgtagagg tcaccgggaa aagttgcgac
     2761 cccatggaag gtcgatctct tcgtttggct ttgcttggct ggcggcattg cgcttcttcg
     2821 cttatacccg tctcttgacg ctcgagctcg ttcattgaga tacctttatt cttgcacatt
     2881 ttctggcttt tttcgctact cgggtacatg taatcatgca cacagaaggt gctgtagggt
     2941 gaaagttcct ttgtgctgtc gtttgttttt aatgccaaac tttccggtga tcaataacca
     3001 cctcattaag acctcagcgc ggccgcaaat ttaaataaaa tgaagtgaag ttcctatact
     3061 ttctagagaa taggaacttc tatagtgagt cgaataaggg cgacacaaaa tttattctaa
     3121 atgcataata aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac
     3181 atgtataatt ttgatatcaa aaactgattt tccctttatt attttcgaga tttattttct
     3241 taattctctt taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa
     3301 tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg
     3361 cttttttctc atttataagg ttaaataatt ctcatatatc aagcaaagtg acaggcgccc
     3421 ttaaatattc tgacaaatgc tctttcccta aactcccccc ataaaaaaac ccgccgaagc
     3481 gggtttttac gttatttgcg gattaacgat tactcgttat cagaaccgcc cagggggccc
     3541 gagcttaaga ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac gcaaaaaggc
     3601 catccgtcag gggccttctg cttagtttga tgcctggcag ttccctactc tcgccttccg
     3661 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3721 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3781 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3841 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3901 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3961 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4021 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4081 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4141 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4201 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tgggctaact
     4261 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4321 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4381 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4441 tttctacggg gtctgacgct cagtggaacg acgcgcgcgt aactcacgtt aagggatttt
     4501 ggtcatgagc ttgcgccgtc ccgtcaagtc agcgtaatgc tctgctttt
//
