LOCUS Exported 4474 bp ds-DNA circular UNA 16-NOV-2016 DEFINITION natural circular DNA ACCESSION BGHA VERSION . KEYWORDS 73274_Genbank SOURCE Saccharomyces cerevisiae ORGANISM Saccharomyces cerevisiae REFERENCE 1 (bases 1 to 4474) AUTHORS Mathew Jessop Fabre TITLE Direct Submission JOURNAL Exported Wednesday, Nov 16, 2016 from SnapGene 3.2.1 http://www.snapgene.com COMMENT ORIGDB|GenBank FEATURES Location/Qualifiers source 1..4474 /organism="Saccharomyces cerevisiae" /mol_type="genomic DNA" CDS complement(1..859) /codon_start=1 /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene complement(1..859) primer_bind 1219..1238 /note="BGHA F1" misc_feature 1304..1310 /note="Nt.BvcI" misc_feature 1318..1779 /note="XI-1 UP" misc_feature 1787..1792 /note="Nt.BvCI" misc_binding 1792..1803 /note="MCS 1" /note="Oriinal location: 1329..1371" misc_feature 1804..1809 /note="Misc_Feature_1" /note="Nb.BsmI" terminator 1817..2011 /note="T ADH1" primer_bind 1835..1854 /note="BGHA Pac1 R3" /note="Primer: GGTCAGGTTGCTTTCTCAGG Number of mismatches: 0 Primer binding region: complement(1373..1392)" misc_feature 2017..2022 /note="Misc_Feature_2" /note="Nb.BsmI" misc_feature 2035..2040 /note="Misc_Feature_3" /note="Nb.BsmI" terminator 2041..2230 /note="T CYC1" misc_feature 2212..2230 /note="primer 1" misc_feature 2245..2262 /note="primer 2" primer_bind 2245..2253 /note="BGHA Pac2 F2" /note="Primer: GGTACCCAATTCGCCCTAT Number of mismatches: 0 Primer binding region: 3360..3378" misc_feature 2340..2346 /note="Nt.BvcI" misc_feature 2354..2929 /note="XI-1 DOWN" misc_feature 2937..2943 /note="Nt.BvcI" primer_bind 3009..3028 /note="BGHA Pac2 R" /note="Primer: TCGCCCTTATTCGACTCACT Number of mismatches: 0 Primer binding region: complement(3556..3575)" rep_origin complement(3729..4372) /direction=LEFT /note="pUC ori" ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatgga 1321 atagtgacgt tgtgatgcgg tgagttcggc ggttagggga atggtatatg ataaaaaacg 1381 gaaacgtgct tctttaattt aattgtttaa tattgttgca gatatataaa aagggggaaa 1441 gaaccgaaga tgtaattatt tttttatcgc ctcaacctaa agcaagcaat aaggtataaa 1501 gatcaggacg tctcgagcgc tgatatctaa atttgaagcc acgcaagtaa ctacgtaggt 1561 cagaggggac aaggaataac acttgacatt tttctttttt cttttttttt cttttttttt 1621 tttttgttaa tcttggcttc tgtaccgtag cctcctgtat acggttttag atgcagtatg 1681 tttatcatcg ccgagaaatt tggggggtgc aaaggaataa aatccgtgct gaaatccgtg 1741 caccgcatca aactttctcg gaggattctt tgcagccgat taagtcctca gcgagctcgc 1801 atggaatgcg tgcgatgagc gacctcatgc tatacctgag aaagcaacct gacctacagg 1861 aaagagttac tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg 1921 tatacactta ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc 1981 gcttatttag aagtgtcaac aacgtatcta ccaacggaat gcgtgcgatc gcgtgcattc 2041 atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt 2101 ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct 2161 gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg 2221 acgctcgaag atcgcgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg 2281 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccctgc aggactagtg 2341 ctgaggcatt aatagtttct tggcattggc aaatctctgc taaatgctgc gtacagacgg 2401 aaactcacac cgccgcgaaa actggtcagt ggcaaaaaaa aaaaaaatta aaaaaataaa 2461 aaataactat tacgtatgat actgtttctg gtagttgata tgaggttggt gttgtatatt 2521 gtacgtttta ggaacaggga agtgaatatt atttactctg ctgcacattc tggctaggtc 2581 gaagccggaa cctgagatga cgccgcgcta gaactatgga ccaagctgtt gacaatgttt 2641 agatggtgat gcactaccct gtgcggggag tggccacgaa cgcgagcgga aggtgcgggt 2701 gttgcgggaa ttgcgggagg ttcttcgcta agcgtgaggg ttgctagttg gggcggcggg 2761 gtttccctaa gtgtaaatag ggcctcgccg ctggcacatg agtgcgccgg aggaggcggc 2821 ggaggcgacg acgctaaaac cgtggccgtt ggggaaggat gggcggctat atctaccatt 2881 gacctgatgg ggactcggct cttaaggaat gggtttgagg tgggtgtgga ttaagacctc 2941 agcgcggccg caaatttaaa taaaatgaag tgaagttcct atactttcta gagaatagga 3001 acttctatag tgagtcgaat aagggcgaca caaaatttat tctaaatgca taataaatac 3061 tgataacatc ttatagtttg tattatattt tgtattatcg ttgacatgta taattttgat 3121 atcaaaaact gattttccct ttattatttt cgagatttat tttcttaatt ctctttaaca 3181 aactagaaat attgtatata caaaaaatca taaataatag atgaatagtt taattatagg 3241 tgttcatcaa tcgaaaaagc aacgtatctt atttaaagtg cgttgctttt ttctcattta 3301 taaggttaaa taattctcat atatcaagca aagtgacagg cgcccttaaa tattctgaca 3361 aatgctcttt ccctaaactc cccccataaa aaaacccgcc gaagcgggtt tttacgttat 3421 ttgcggatta acgattactc gttatcagaa ccgcccaggg ggcccgagct taagactggc 3481 cgtcgtttta caacacagaa agagtttgta gaaacgcaaa aaggccatcc gtcaggggcc 3541 ttctgcttag tttgatgcct ggcagttccc tactctcgcc ttccgcttcc tcgctcactg 3601 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 3661 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 3721 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 3781 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 3841 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 3901 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 3961 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 4021 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 4081 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 4141 ggtatgtagg cggtgctaca gagttcttga agtggtgggc taactacggc tacactagaa 4201 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 4261 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 4321 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 4381 acgctcagtg gaacgacgcg cgcgtaactc acgttaaggg attttggtca tgagcttgcg 4441 ccgtcccgtc aagtcagcgt aatgctctgc tttt //