LOCUS       pCfB2903\(XI-2\MarkerFree)        4569 bp    DNA     circular UNA  7-AUG-2015
ACCESSION   BGHA URA3 ADH1 CYC1 Nb.BsmI
SOURCE      Saccharomyces cerevisiae (unknown).
  ORGANISM  Saccharomyces cerevisiae
            Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|741616367|
COMMENT     VNTDBDATE|741616367|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB2903 (XI-2 MarkerFree)|
COMMENT     VNTAUTHORNAME|Mathew Jessop Fabre|
COMMENT     VNTAUTHOREML|mmjf@biosustain.dtu.dk|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/BGHA\">BGHA</a>; <a href=\"ncbilink:dnarna/URA3\">URA3</a>; <a href=\"ncbilink:dnarna/ADH1\">ADH1</a>; <a href=\"ncbilink:dnarna/CYC1\">CYC1</a>; <a href=\"ncbilink:dnarna/Nb.BsmI\">Nb.BsmI</a>" 
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COMMENT                          "Sequence Source: Saccharomyces cerevisiae (unknown)." 
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FEATURES             Location/Qualifiers
     gene            complement(1..859)
                     /vntifkey="60"
     primer          1167..1186
                     /vntifkey="27"
                     /label=BGHA\Pac1\F2
                     /note="PCR on col primer"
     primer_bind     1219..1238
                     /vntifkey="28"
                     /label=BGHA\F1
     misc_feature    complement(1304..1310)
                     /vntifkey="21"
                     /label=Nt.BvcI
     misc_feature    1318..1886
                     /vntifkey="21"
                     /label=XI-2\UP
     misc_feature    1894..1900
                     /vntifkey="21"
                     /label=Nt.BvCI
     misc_binding    1900..1911
                     /vntifkey="20"
                     /label=MCS\1
                     /note="Oriinal location: 1329..1371"
     misc_feature    1912..1917
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Nb.BsmI"
     terminator      complement(1925..2119)
                     /vntifkey="43"
                     /label=T\ADH1
     primer_bind     complement(1943..1962)
                     /vntifkey="28"
                     /label=BGHA\Pac1\R3
                     /note="Primer: GGTCAGGTTGCTTTCTCAGG Number of mismatches: 0  Primer binding region: complement(1373..1392)"
     misc_feature    2125..2130
                     /vntifkey="21"
                     /label=Misc_Feature_2
                     /note="Nb.BsmI"
     misc_feature    2143..2148
                     /vntifkey="21"
                     /label=Misc_Feature_3
                     /note="Nb.BsmI"
     terminator      2149..2338
                     /vntifkey="43"
                     /label=T\CYC1
     primer_bind     2353..2361
                     /vntifkey="28"
                     /label=BGHA\Pac2\F2
                     /note="Primer: GGTACCCAATTCGCCCTAT Number of mismatches: 0  Primer binding region: 3360..3378"
     misc_feature    2448..2454
                     /vntifkey="21"
                     /label=Nt.BvcI
     misc_feature    2462..3024
                     /vntifkey="21"
                     /label=XI-2\Down
     misc_feature    3032..3038
                     /vntifkey="21"
                     /label=Nt.BvcI
     primer_bind     complement(3104..3123)
                     /vntifkey="28"
                     /label=BGHA\Pac2\R
                     /note="Primer: TCGCCCTTATTCGACTCACT Number of mismatches: 0  Primer binding region: complement(3556..3575)"
     rep_origin      complement(3824..4467)
                     /vntifkey="33"
                     /label=pUC\ori
     CDS             complement(1..859)
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    2320..2338
                     /vntifkey="21"
                     /label=primer\1
     misc_feature    2353..2370
                     /vntifkey="21"
                     /label=primer\2
BASE COUNT     1228 a      1047 c       998 g      1296 t 
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatgag 
     1321 gattttcgat ggagcaggat gaggagaaat agtaccacat gtatatatcc attacaaaaa 
     1381 ggtttatata caattacaat agacccttgt tggggtttct gaaaaaagaa gtagtcgatg 
     1441 ccatcggcaa taatacggaa ttacgagaaa cacaatcccg atcctttttt gggtaattac 
     1501 ttcaccgatt ctaccgattt atcatgccaa aaaaaattca ccgtgggttc tagaagtgcc 
     1561 ctttgaggat tgtagccact ctaacccaca cggcctcctt actagctgac taaggtgaca 
     1621 aaaccgcaag gactggaaag tcgccactca tctgaaaatt ctcaagtttt tcactactga 
     1681 gtttatgctt tcgaattttt ttgttcggta atagcacggc ggttcgattc aattccgccg 
     1741 ctccgagcga tgctccgcaa aactcagtaa taagctttct gatggttcac ccctttttta 
     1801 gcacgcgggg tgtaactcaa cagaaaaatg tgccatagaa caagactagg caaaagccaa 
     1861 ggagcgtttg ccatgaactt ccacaaatta agtcctcagc gagctcgcat ggaatgcgtg 
     1921 cgatgagcga cctcatgcta tacctgagaa agcaacctga cctacaggaa agagttactc 
     1981 aagaataaga attttcgttt taaaacctaa gagtcacttt aaaatttgta tacacttatt 
     2041 ttttttataa cttatttaat aataaaaatc ataaatcata agaaattcgc ttatttagaa 
     2101 gtgtcaacaa cgtatctacc aacggaatgc gtgcgatcgc gtgcattcat ccgctctaac 
     2161 cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat 
     2221 gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt 
     2281 gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaagat 
     2341 cgcgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta 
     2401 caacgtcgtg actgggaaaa ccctggcgtt acccctgcag gactagtgct gaggcattaa 
     2461 tcacacgact agcgctttca gatattaaaa agtttagatg taggttttag cggtaacagt 
     2521 tatataaatc gtgtttcttc tcttgatgaa acaaaaaaat gctagaaaaa ctttgtcgtt 
     2581 tcttactttt ggtgcgcttt gcagttttcg tggctagact tagaatcatt tctcctcaga 
     2641 tttcttgatt aaagtttggt gcgaagccct actctaacat tggtgttctt cttttcattc 
     2701 acgcaagtta agtccaggaa ggtgagcaaa tgctcatcct tctgttcatg cgtgacggct 
     2761 gaattatcct tatctggcgt acccgtgcag ccgtttccgt gcctcggttc ctccgagata 
     2821 tccttaggga ccgccaggga ccatgattgc gtcaactgtt gtcaccgctc cagaggatcc 
     2881 tctgtaacct tttcaaccat aaaaatagaa agggcacagg gttggggtat ttgaattttt 
     2941 tttttgggtt ttttcgtatt acttattact taccttcttt tctatataat tttgtttttc 
     3001 cctggtagag cggaatcttc ccacattaag acctcagcgc ggccgcaaat ttaaataaaa 
     3061 tgaagtgaag ttcctatact ttctagagaa taggaacttc tatagtgagt cgaataaggg 
     3121 cgacacaaaa tttattctaa atgcataata aatactgata acatcttata gtttgtatta 
     3181 tattttgtat tatcgttgac atgtataatt ttgatatcaa aaactgattt tccctttatt 
     3241 attttcgaga tttattttct taattctctt taacaaacta gaaatattgt atatacaaaa 
     3301 aatcataaat aatagatgaa tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt 
     3361 atcttattta aagtgcgttg cttttttctc atttataagg ttaaataatt ctcatatatc 
     3421 aagcaaagtg acaggcgccc ttaaatattc tgacaaatgc tctttcccta aactcccccc 
     3481 ataaaaaaac ccgccgaagc gggtttttac gttatttgcg gattaacgat tactcgttat 
     3541 cagaaccgcc cagggggccc gagcttaaga ctggccgtcg ttttacaaca cagaaagagt 
     3601 ttgtagaaac gcaaaaaggc catccgtcag gggccttctg cttagtttga tgcctggcag 
     3661 ttccctactc tcgccttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     3721 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     3781 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     3841 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     3901 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     3961 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     4021 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     4081 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     4141 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     4201 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     4261 cttgaagtgg tgggctaact acggctacac tagaagaaca gtatttggta tctgcgctct 
     4321 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     4381 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     4441 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgcgcgt 
     4501 aactcacgtt aagggatttt ggtcatgagc ttgcgccgtc ccgtcaagtc agcgtaatgc 
     4561 tctgctttt 
//
