LOCUS Exported 4547 bp ds-DNA circular UNA 16-NOV-2016 DEFINITION natural circular DNA ACCESSION BGHA VERSION . KEYWORDS 73276_Genbank SOURCE Saccharomyces cerevisiae ORGANISM Saccharomyces cerevisiae REFERENCE 1 (bases 1 to 4547) AUTHORS Mathew Jessop Fabre TITLE Direct Submission JOURNAL Exported Wednesday, Nov 16, 2016 from SnapGene 3.2.1 http://www.snapgene.com COMMENT ORIGDB|GenBank FEATURES Location/Qualifiers source 1..4547 /organism="Saccharomyces cerevisiae" /mol_type="genomic DNA" gene complement(1..859) CDS complement(1..859) /codon_start=1 /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1219..1238 /note="BGHA F1" misc_feature 1304..1310 /note="Nt.BvcI" misc_feature 1318..1892 /note="XI-3 UP" misc_feature 1900..1906 /note="Nt.BvCI" misc_binding 1906..1917 /note="MCS 1" /note="Oriinal location: 1329..1371" misc_feature 1918..1923 /note="Misc_Feature_1" /note="Nb.BsmI" terminator 1931..2125 /note="T ADH1" primer_bind 1949..1968 /note="BGHA Pac1 R3" /note="Primer: GGTCAGGTTGCTTTCTCAGG Number of mismatches: 0 Primer binding region: complement(1373..1392)" misc_feature 2131..2136 /note="Misc_Feature_2" /note="Nb.BsmI" misc_feature 2149..2154 /note="Misc_Feature_3" /note="Nb.BsmI" terminator 2155..2344 /note="T CYC1" primer_bind 2358..2366 /note="BGHA Pac2 F2" /note="Primer: GGTACCCAATTCGCCCTAT Number of mismatches: 0 Primer binding region: 3360..3378" misc_feature 2453..2459 /note="Nt.BvcI" misc_feature 2467..3002 /note="XI-3 Down" misc_feature 3010..3016 /note="Nt.BvcI" primer_bind 3082..3101 /note="BGHA Pac2 R" /note="Primer: TCGCCCTTATTCGACTCACT Number of mismatches: 0 Primer binding region: complement(3556..3575)" rep_origin complement(3802..4445) /direction=LEFT /note="pUC ori" ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatagt 1321 tacttgctct atgcgtttgc gcatcctctt tttacttttt ttttttcagt aaagcctaag 1381 cataaatcgt tttatacgta cgacacgttc aacttttctt ggttagtagt ggcaatctct 1441 gcaatacata cagggagtca tggtctatca tcttgtccaa tcaaagaagc atcggttcag 1501 atcgagcaaa ctgtagggag aaaggaaagt agaaatgcag agtgtgctat atgtccaatc 1561 tcggttttgt agtttggatg tcattagaga tctaccaccc aaccggctgc tttcatgtgg 1621 aacagaaaag aaatcggggc gcttcctctt ctgtattcct ttagttaacg tttttattca 1681 gccatctaac catcataccc ccatacggta acaaaacctc ttctaagaaa agaagtctct 1741 gctcctccgc catcttattt ttattcgctg cgcgcgttta ttgtcgcatc gctggccagc 1801 aaaaaattgg ttgccttttt ttacctaaaa aagacacatc taactgatta gttttccgtt 1861 ttaggatatt gacgccaagc gtgcgtctga ttattaagtc ctcagcgagc tcgcatggaa 1921 tgcgtgcgat gagcgacctc atgctatacc tgagaaagca acctgaccta caggaaagag 1981 ttactcaaga ataagaattt tcgttttaaa acctaagagt cactttaaaa tttgtataca 2041 cttatttttt ttataactta tttaataata aaaatcataa atcataagaa attcgcttat 2101 ttagaagtgt caacaacgta tctaccaacg gaatgcgtgc gatcgcgtgc attcatccgc 2161 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat 2221 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag 2281 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc 2341 gaagatcgcg tcccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg 2401 ttttacaacg tcgtgactgg gaaaaccctg gcgttacccc tgcaggacta gtgctgaggc 2461 attaatttac gtggattgag ccagcaatac agatcattat taaattgttt tgtacatgat 2521 gttagtatat aatcgtaaag cttttctaat atgtatacct tatacatgga actccacaga 2581 acttgcaaac ataccaaaaa tcccttattc ttgttcactc attttacatc aaaaaataat 2641 atttcagtta ttaaggaaaa taaaaaaata gattagagaa gcattttgaa gaaatagtat 2701 attcttttat tgaacctaag agcgtgatat ttttactcga aataaaatac gaaaaatcta 2761 tgcactcatc tttccgacta ctattggctc ctgctcaaaa aaagagggaa aaaaagctcc 2821 aaaattctat cttttcctat cgctcctgtc ctatccttat tacgttcatt actattttaa 2881 tactatccat tcttttattt tcagtctaaa aaaaacattt ctcataacgg gaaaagcaaa 2941 aaaatgtcaa gcttatacat caaaacacca ctgcatgcat tatctgctgg tccggattct 3001 caattaagac ctcagcgcgg ccgcaaattt aaataaaatg aagtgaagtt cctatacttt 3061 ctagagaata ggaacttcta tagtgagtcg aataagggcg acacaaaatt tattctaaat 3121 gcataataaa tactgataac atcttatagt ttgtattata ttttgtatta tcgttgacat 3181 gtataatttt gatatcaaaa actgattttc cctttattat tttcgagatt tattttctta 3241 attctcttta acaaactaga aatattgtat atacaaaaaa tcataaataa tagatgaata 3301 gtttaattat aggtgttcat caatcgaaaa agcaacgtat cttatttaaa gtgcgttgct 3361 tttttctcat ttataaggtt aaataattct catatatcaa gcaaagtgac aggcgccctt 3421 aaatattctg acaaatgctc tttccctaaa ctccccccat aaaaaaaccc gccgaagcgg 3481 gtttttacgt tatttgcgga ttaacgatta ctcgttatca gaaccgccca gggggcccga 3541 gcttaagact ggccgtcgtt ttacaacaca gaaagagttt gtagaaacgc aaaaaggcca 3601 tccgtcaggg gccttctgct tagtttgatg cctggcagtt ccctactctc gccttccgct 3661 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 3721 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 3781 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 3841 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 3901 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 3961 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 4021 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 4081 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 4141 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 4201 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg ggctaactac 4261 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 4321 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 4381 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 4441 tctacggggt ctgacgctca gtggaacgac gcgcgcgtaa ctcacgttaa gggattttgg 4501 tcatgagctt gcgccgtccc gtcaagtcag cgtaatgctc tgctttt //