LOCUS       pCfB3039\(XII-2\MarkerFree)        4521 bp    DNA     circular      7-AUG-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|741619992|
COMMENT     VNTDBDATE|741619992|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB3039 (XII-2 MarkerFree)|
COMMENT     VNTAUTHORNAME|Mathew Jessop Fabre|
COMMENT     VNTAUTHOREML|mmjf@biosustain.dtu.dk|
COMMENT     VNTOAUTHORNAME|Kanchana Kildegaard|
COMMENT     VNTOAUTHOREML|karu@biosustain.dtu.dk|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Currently local object. Original author: Kanchana Kildegaard" 
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FEATURES             Location/Qualifiers
     CDS             complement(64..924)
                     /vntifkey="4"
                     /label=AmpR
     terminator      2180..2368
                     /vntifkey="43"
                     /label=T\CYC1
     rep_origin      3818..4483
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    1383..1917
                     /vntifkey="21"
                     /label=XII-2\UP
     terminator      1958..2118
                     /vntifkey="43"
                     /label=T\ADH1
     misc_feature    2493..3041
                     /vntifkey="21"
                     /label=XII-2\Down
     misc_feature    2384..2401
                     /vntifkey="21"
                     /label=primer1
     misc_feature    2351..2369
                     /vntifkey="21"
                     /label=primer2
BASE COUNT     1240 a      1030 c       962 g      1289 t 
ORIGIN
        1 cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg 
       61 cttttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
      121 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
      181 ccccagcgct gcgatgatac cgcgagaacc acgctcaccg gctccggatt tatcagcaat 
      241 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
      301 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
      361 caacgttgtt gccatcgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
      421 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
      481 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
      541 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
      601 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
      661 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
      721 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
      781 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
      841 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
      901 gacacggaaa tgttgaatac tcatattctt cctttttcaa tattattgaa gcatttatca 
      961 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     1021 ggtcagtgtt acaaccaatt aaccaattct gaacattatc gcgagcccat ttatacctga 
     1081 atatggctca taacacccct tgtttgcctg gcggcagtag cgcggtggtc ccacctgacc 
     1141 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg actccccatg 
     1201 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 
     1261 tttcgcccgg gctaattatg gggtgtcgcc cttattcgac tctatagtga agttcctatt 
     1321 ctctagaaag tataggaact tctgaagtgg ggatttaaat gcggccgcgc tgagggttta 
     1381 atcggcatgc aaacatctac acaattagca agggcaatcc atattttgtc ttttcgcgcc 
     1441 ctggaaaggc ctaagtaatg tcgtaaacgc attctatctg tacttcaact ctcctctgtg 
     1501 cattggtttg tgcaaatcac attttacgat actgccagat atatgcaaaa agagaaaacc 
     1561 aagggaccag aacaaagcaa aattacgata ttcttcgaat tccttcgtgc ttgactaaga 
     1621 caaagggatg gacgtagcga tttttagcgg gccaagaact ggttccgaaa aagcacaggt 
     1681 acaccgaacc ctcagctaag gagggacagc accgatgcgg aaggacaaac tttctttttg 
     1741 cctatcacag tatcttatcg agctaactat tttcgacaca catgaaaaag cagaaatatt 
     1801 aacgaaaaag aaaagaaaga ccatgtcatg tacgggcaat cagaatctgt aacaagcgcc 
     1861 attttttttt ctgtatcggg ccctccttac tgctctcctt ccgtgtaacg cgttatgatt 
     1921 aagtcctcag cgagctcgca tggaatgcgt gcgatgagcg acctcatgct atacctgaga 
     1981 aagcaacctg acctacagga aagagttact caagaataag aattttcgtt ttaaaaccta 
     2041 agagtcactt taaaatttgt atacacttat tttttttata acttatttaa taataaaaat 
     2101 cataaatcat aagaaattcg cttatttaga agtgtcaaca acgtatctac caacggaatg 
     2161 cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga caacctgaag 
     2221 tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc 
     2281 aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct 
     2341 tgcttgagaa ggttttggga cgctcgaaga tcgcgtaccc aattcgccct atagtgagtc 
     2401 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt 
     2461 tacccctgca ggactagtgc tgaggcatta atcgactctc tcgaaatttt tcttaacgcg 
     2521 tccttgtact gcgtctaacg cttttgccac ttggatttct attataggaa atagtctcac 
     2581 ttactgggcg acgaattttc gcgttttgat gaagcacagg aagaatttct tttttttttg 
     2641 gcttcttctg gttccgtttt ttacgcgcac aaatctaaaa aaagaaataa ttataaccta 
     2701 gtctcgaaaa ttttcatcga tccattcgtt cctttttttc gattttttca gatcaaaatt 
     2761 cttgtttctt tctttgtctt agtttatatt aaaagatatt ttgattttac tcctgaacta 
     2821 tttattcttt ctaagaaggc cagaacacta cagctgtttt aaccgactac gaagttctcc 
     2881 attctcgaac actagccttc atttaccaaa caggaactag cgtatatcat tagtccttat 
     2941 tcgaaaagag attggtagat atttattgta gtttgtgaga aggagaaaat actgtcattg 
     3001 gactgatagt tagaggacat taacctctct tacgttcgct cattaagacc tcagcgcggc 
     3061 cgcaaattta aataaaatga agtgaagttc ctatactttc tagagaatag gaacttctat 
     3121 agtgagtcga ataagggcga cacaaaattt attctaaatg cataataaat actgataaca 
     3181 tcttatagtt tgtattatat tttgtattat cgttgacatg tataattttg atatcaaaaa 
     3241 ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa caaactagaa 
     3301 atattgtata tacaaaaaat cataaataat agatgaatag tttaattata ggtgttcatc 
     3361 aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt tataaggtta 
     3421 aataattctc atatatcaag caaagtgaca ggcgccctta aatattctga caaatgctct 
     3481 ttccctaaac tccccccata aaaaaacccg ccgaagcggg tttttacgtt atttgcggat 
     3541 taacgattac tcgttatcag aaccgcccag ggggcccgag cttaagactg gccgtcgttt 
     3601 tacaacacag aaagagtttg tagaaacgca aaaaggccat ccgtcagggg ccttctgctt 
     3661 agtttgatgc ctggcagttc cctactctcg ccttccgctt cctcgctcac tgactcgctg 
     3721 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     3781 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     3841 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     3901 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     3961 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     4021 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     4081 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     4141 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     4201 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     4261 ggcggtgcta cagagttctt gaagtggtgg gctaactacg gctacactag aagaacagta 
     4321 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     4381 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     4441 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     4501 tggaacgacg cgcgcgtaac t 
//
