LOCUS       Exported                4433 bp ds-DNA     circular UNA 16-NOV-2016
DEFINITION  natural circular DNA
ACCESSION   BGHA
VERSION     .
KEYWORDS    73280_Genbank
SOURCE      Saccharomyces cerevisiae
  ORGANISM  Saccharomyces cerevisiae
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Mathew Jessop Fabre
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 16, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
     gene            complement(1..859)
     CDS             complement(1..859)
                     /codon_start=1
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1219..1238
                     /note="BGHA F1"
     misc_feature    1304..1310
                     /note="Nt.BvcI"
     misc_feature    1318..1820
                     /note="XII-4 UP"
     misc_feature    1828..1834
                     /note="Nt.BvCI"
     misc_binding    1834..1845
                     /note="MCS 1"
                     /note="Oriinal location: 1329..1371"
     misc_feature    1846..1851
                     /note="Misc_Feature_1"
                     /note="Nb.BsmI"
     terminator      1859..2053
                     /note="T ADH1"
     primer_bind     1877..1896
                     /note="BGHA Pac1 R3"
                     /note="Primer: GGTCAGGTTGCTTTCTCAGG Number of mismatches: 0
                      Primer binding region: complement(1373..1392)"
     misc_feature    2059..2064
                     /note="Misc_Feature_2"
                     /note="Nb.BsmI"
     misc_feature    2077..2082
                     /note="Misc_Feature_3"
                     /note="Nb.BsmI"
     terminator      2083..2272
                     /note="T CYC1"
     misc_feature    2254..2272
                     /note="primer 1"
     misc_feature    2286..2303
                     /note="primer 2"
     primer_bind     2286..2294
                     /note="BGHA Pac2 F2"
                     /note="Primer: GGTACCCAATTCGCCCTAT Number of mismatches: 0 
                     Primer binding region: 3360..3378"
     misc_feature    2381..2387
                     /note="Nt.BvcI"
     misc_feature    2395..2888
                     /note="XII-4 DOWN"
     misc_feature    2896..2902
                     /note="Nt.BvcI"
     primer_bind     2968..2987
                     /note="BGHA Pac2 R"
                     /note="Primer: TCGCCCTTATTCGACTCACT Number of mismatches: 0
                      Primer binding region: complement(3556..3575)"
     rep_origin      complement(3688..4331)
                     /direction=LEFT
                     /note="pUC ori"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatgta
     1321 tccggctgtt ccttcatagc cctttcaatg aacgttgcag ccctttgaag attggccatt
     1381 ttgtcaggac tcgagcctga cagttggacc aacgcaactt taattttttg tgaaagaatc
     1441 ttcgaagcac tcatactggc gatcttcacg ccctcctgct attacaaaag ctgtgttttt
     1501 acaagaatca aattaagtta gcaagatatt atacaacatt attgataatt tcaatatcgt
     1561 gttcgtacct gatgacgtat ctgtgcattg ataaggcccg catggtttca gaaagcagag
     1621 cggaacgatt ccaaattagt ggccttgtgc tttgcatgtc aattgtgtta ccttcagctc
     1681 gtggatttgt tttatcaata cacagtctac agtcaagaat tttttttatc aaattttgcg
     1741 ttcgagcgta taaaatagcc gctgtagcta cttaagttcc tgttcagcga tagttttttt
     1801 ccatcacacg tactatggca attaagtcct cagcgagctc gcatggaatg cgtgcgatga
     1861 gcgacctcat gctatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaat
     1921 aagaattttc gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt
     1981 ataacttatt taataataaa aatcataaat cataagaaat tcgcttattt agaagtgtca
     2041 acaacgtatc taccaacgga atgcgtgcga tcgcgtgcat tcatccgctc taaccgaaaa
     2101 ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt
     2161 attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc
     2221 atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga agatcgcgtc
     2281 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     2341 gtgactggga aaaccctggc gttacccctg caggactagt gctgaggcat taatagttta
     2401 ctcaattctt gaagccaatt tgtacaattc cccattagag tcaaataaaa ggatgcctca
     2461 cggaggtatg ttacccgcgc tatttcacat ggctcattga attagaggtg gaatttggtg
     2521 taccctcccc tcctcatctg atgaagtagt gatccgacaa ttcttaaaag ttgtagacat
     2581 tacttttacc accaactaag ttgtatttat attgctaccc ttatcctttt atatctaact
     2641 agcgctcata aggttggggc aatactaaaa ctgtgttctt attcaactca ttaaatacgt
     2701 ggcagtacgt accctattag aaacaatagg aaacagcaga gtcggaagaa gccaaatgcc
     2761 agatttgaag tccaaaacct tgtcaagcca atctttggga gcggctattc ctccagaaat
     2821 tgtgtaccaa atacttacat accagtttag ggatttgtta agaaatgacc atccaggtac
     2881 ggcagaaaat taagacctca gcgcggccgc aaatttaaat aaaatgaagt gaagttccta
     2941 tactttctag agaataggaa cttctatagt gagtcgaata agggcgacac aaaatttatt
     3001 ctaaatgcat aataaatact gataacatct tatagtttgt attatatttt gtattatcgt
     3061 tgacatgtat aattttgata tcaaaaactg attttccctt tattattttc gagatttatt
     3121 ttcttaattc tctttaacaa actagaaata ttgtatatac aaaaaatcat aaataataga
     3181 tgaatagttt aattataggt gttcatcaat cgaaaaagca acgtatctta tttaaagtgc
     3241 gttgcttttt tctcatttat aaggttaaat aattctcata tatcaagcaa agtgacaggc
     3301 gcccttaaat attctgacaa atgctctttc cctaaactcc ccccataaaa aaacccgccg
     3361 aagcgggttt ttacgttatt tgcggattaa cgattactcg ttatcagaac cgcccagggg
     3421 gcccgagctt aagactggcc gtcgttttac aacacagaaa gagtttgtag aaacgcaaaa
     3481 aggccatccg tcaggggcct tctgcttagt ttgatgcctg gcagttccct actctcgcct
     3541 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3601 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3661 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3721 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3781 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3841 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3901 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3961 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4021 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4081 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtgggct
     4141 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4201 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4261 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4321 atcttttcta cggggtctga cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga
     4381 ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta atgctctgct ttt
//
