LOCUS Exported 5293 bp ds-DNA circular SYN 16-NOV-2016 DEFINITION Cloning vector pESC-LEU, complete sequence. ACCESSION AF063849 VERSION . KEYWORDS 73282_Genbank SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5293) AUTHORS Lu Q. TITLE pESC-LEU JOURNAL Unpublished REFERENCE 2 (bases 1 to 5293) AUTHORS Lu Q. TITLE Direct Submission JOURNAL Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA REFERENCE 3 (bases 1 to 5293) AUTHORS Addgene TITLE Direct Submission JOURNAL Exported Wednesday, Nov 16, 2016 from SnapGene 3.2.1 http://www.snapgene.com COMMENT ApEinfo:methylated:1 ORIGDB|GenBank FEATURES Location/Qualifiers source 1..5293 /organism="synthetic DNA construct" /mol_type="other DNA" source 131..1340 /organism="unspecified" /mol_type="genomic DNA" source join(1452..1740,1761..1879) /organism="p426-SNR52p-gRNA.CAN1.Y-SUP4t" /mol_type="other DNA" misc_feature 1..20 /note="TJOS-101R" misc_feature 29..48 /note="TJOS-101F" misc_feature 99..118 /note="TJOS-102R" misc_signal 131..1340 /gene="(null)" /note="(null)" misc_feature 131..517 /gene="TEF1p" /product="TEF1 promoter of A. gossypii" /note="TEF1 promoter of A. gossypii" CDS 519..1091 /codon_start=1 /gene="nat" /product="Nourseothricin acetyltransferase" /note="Nourseothricin acetyltransferase" /note="nat" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTTTGDGF TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT LCGLDTALYDGTASDGEQALYMSMPCP" misc_feature 1092..1340 /gene="TEF1t" /product="TEF1 terminator of A. gossypii" /note="TEF1 terminator of A. gossypii" misc_feature 1349..1368 /note="TJOS-102F" misc_feature 1419..1438 /note="TJOS-97R" misc_feature 1433..1438 /note="PvuII" misc_feature join(1439..1451,1882..1892) /note="USER site" misc_feature 1452..1471 /note="Forward primer for USER integration" promoter 1452..1455 /note="T3_promoter" promoter 1472..1740 /note="SNR52 Promoter" misc_feature 1714..1740 /note="TJOS20" misc_feature 1761..1839 /note="Structural guiding RNA" misc_feature 1840..1859 /note="SUP4 Terminator" misc_feature 1860..1879 /note="Reverse primer for USER integration" misc_feature 1893..1912 /note="TJOS-97F" misc_feature 1893..1898 /note="PvuII(1)" rep_origin 2076..2743 /direction=RIGHT /note="pUC ori" CDS complement(2894..3751) /codon_start=1 /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3885..5040 /direction=RIGHT /note="2 micron ori" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggct 121 gcatttaaat gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 181 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 241 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 301 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 361 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 421 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 481 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaccact cttgacgaca 541 cggcttaccg gtaccgcacc agtgtcccgg gggacgccga ggccatcgag gcactggatg 601 ggtccttcac caccgacacc gttttccgcg tcaccaccac cggggacggc ttcaccctgc 661 gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa tcggacgacg 721 aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg tacggggacg 781 acggcgacct ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg 841 tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg 901 ggctcgcgac ggagttcgcc cgcgagcggg gcgccgggca cctctggctg gaggtcacca 961 acgtcaacgc accggcgatc cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg 1021 acaccgccct gtacgacggc accgcctcgg acggcgagca ggcgctctac atgagcatgc 1081 cctgccccta atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt 1141 atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt 1201 ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg 1261 cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca 1321 tccagtgtcg aaaacgagct gcggccgcct acagggcgcg tcgcgccatt cgccattcag 1381 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctgga 1441 atgcgtgcga tagggaacaa aagctggagc ttctttgaaa agataatgta tgattatgct 1501 ttcactcata tttatacaga aacttgatgt tttctttcga gtatatacaa ggtgattaca 1561 tgtacgtttg aagtacaact ctagattttg tagtgccctc ttgggctagc ggtaaaggtg 1621 cgcatttttt cacaccctac aatgttctgt tcaaaagatt ttggtcaaac gctgtagaag 1681 tgaaagttgg tgcgcatgtt tcggcgttcg aaacttctcc gcagtgaaag ataaatgatc 1741 ctctcgaagt ggtcacgtgc gttttagagc tagaaatagc aagttaaaat aaggctagtc 1801 cgttatcaac ttgaaaaagt ggcaccgagt cggtggtgct ttttttgttt tttatgtctt 1861 cgagtcatgt aattagttaa tcgcgtgcat tccagctgca ttaatgaatc ggccaacgcg 1921 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 1981 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 2041 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 2101 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 2161 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 2221 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 2281 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 2341 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 2401 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 2461 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 2521 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 2581 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 2641 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 2701 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 2761 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 2821 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 2881 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 2941 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 3001 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 3061 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 3121 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 3181 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 3241 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 3301 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 3361 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 3421 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 3481 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 3541 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 3601 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 3661 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 3721 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 3781 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 3841 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt 3901 cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc 3961 tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt 4021 gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc 4081 tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa 4141 tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag 4201 catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt 4261 ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca 4321 taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat 4381 tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt 4441 gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc 4501 ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga 4561 ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa 4621 acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt 4681 aggttatata gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt 4741 gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt 4801 ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt 4861 tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc 4921 gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg 4981 tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg 5041 cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt 5101 atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata 5161 tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt 5221 ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 5281 aggccctttc gtc //