LOCUS       Exported                5285 bp ds-DNA     circular SYN 16-NOV-2016
DEFINITION  Cloning vector pESC-LEU, complete sequence.
ACCESSION   AF063849
VERSION     .
KEYWORDS    73286_Genbank
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Lu Q.
  TITLE     pESC-LEU
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 5285)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 16, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     ApEinfo:methylated:1
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          127..1336
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..20
                     /note="TJOS-101R"
     misc_feature    29..48
                     /note="TJOS-101F"
     misc_feature    99..118
                     /note="TJOS-102R"
     misc_signal     127..1336
                     /gene="(null)"
                     /note="(null)"
     misc_feature    127..513
                     /gene="TEF1p"
                     /product="TEF1 promoter of A. gossypii"
                     /note="TEF1 promoter of A. gossypii"
     CDS             515..1087
                     /codon_start=1
                     /gene="nat"
                     /product="Nourseothricin acetyltransferase"
                     /note="Nourseothricin acetyltransferase"
                     /note="nat"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     misc_feature    1088..1336
                     /gene="TEF1t"
                     /product="TEF1 terminator of A. gossypii"
                     /note="TEF1 terminator of A. gossypii"
     misc_feature    1345..1360
                     /note="TJOS-102F"
     misc_feature    1411..1430
                     /note="TJOS-97R"
     misc_feature    1425..1430
                     /note="PvuII"
     misc_feature    1444..1463
                     /note="Forward primer for USER integration"
     promoter        1444..1447
                     /note="T3_promoter"
     promoter        1464..1732
                     /note="SNR52 Promoter"
     misc_feature    1706..1732
                     /note="TJOS20"
     misc_feature    1753..1831
                     /note="Structural guiding RNA"
     misc_feature    1832..1851
                     /note="SUP4 Terminator"
     misc_feature    1852..1871
                     /note="Reverse primer for USER integration"
     misc_feature    1885..1904
                     /note="TJOS-97F"
     misc_feature    1885..1890
                     /note="PvuII(1)"
     rep_origin      2068..2735
                     /direction=RIGHT
                     /note="pUC ori"
     CDS             complement(2886..3743)
                     /codon_start=1
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3877..5032
                     /direction=RIGHT
                     /note="2 micron ori"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggat
      121 ttaaatgatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
      181 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
      241 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
      301 gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg
      361 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
      421 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
      481 acagttctca catcacatcc gaacataaac aaccatgggt accactcttg acgacacggc
      541 ttaccggtac cgcaccagtg tcccggggga cgccgaggcc atcgaggcac tggatgggtc
      601 cttcaccacc gacaccgttt tccgcgtcac cgccaccggg gacggcttca ccctgcggga
      661 ggtgccggtg gacccgcccc tgaccaaggt gttccccgac gacgaatcgg acgacgaatc
      721 ggacgacggg gaggacggcg acccggactc ccggacgttc gtcgcgtacg gggacgacgg
      781 cgacctggcg ggcttcgtgg tcgtctcgta ctccggctgg aaccgccggc tgaccgtcga
      841 ggacatcgag gtcgccccgg agcaccgggg gcacggggtc gggcgcgcgt tgatggggct
      901 cgcgacggag ttcgcccgcg agcggggcgc cgggcacctc tggctggagg tcaccaacgt
      961 caacgcaccg gcgatccacg cgtaccggcg gatggggttc accctctgcg gcctggacac
     1021 cgccctgtac gacggcaccg cctcggacgg cgagcaggcg ctctacatga gcatgccctg
     1081 cccctaatca gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag
     1141 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt
     1201 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc
     1261 aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca
     1321 gtgtcgaaaa cgagctgcgg ccgcctacag ggcgcgtcca ttcgccattc aggctgcgca
     1381 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gaatgcgtgc
     1441 gatagggaac aaaagctgga gcttctttga aaagataatg tatgattatg ctttcactca
     1501 tatttataca gaaacttgat gttttctttc gagtatatac aaggtgatta catgtacgtt
     1561 tgaagtacaa ctctagattt tgtagtgccc tcttgggcta gcggtaaagg tgcgcatttt
     1621 ttcacaccct acaatgttct gttcaaaaga ttttggtcaa acgctgtaga agtgaaagtt
     1681 ggtgcgcatg tttcggcgtt cgaaacttct ccgcagtgaa agataaatga tcgttgacca
     1741 gttgatcagt tggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     1801 acttgaaaaa gtggcaccga gtcggtggtg ctttttttgt tttttatgtc ttcgagtcat
     1861 gtaattagtt aatcgcgtgc attccagctg cattaatgaa tcggccaacg cgcggggaga
     1921 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1981 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2041 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2101 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2161 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2221 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2281 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2341 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2401 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2461 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2521 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     2581 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2641 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2701 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2761 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2821 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2881 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3181 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3481 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3781 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3841 gttccgcgca catttccccg aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt
     3901 agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
     3961 tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt
     4021 tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     4081 atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact
     4141 tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag
     4201 attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg
     4261 taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa
     4321 gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa
     4381 gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag
     4441 aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt
     4501 tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct
     4561 atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa
     4621 aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata
     4681 tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc
     4741 ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt
     4801 gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga
     4861 ataggaactt cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc
     4921 aacgcgagct gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct
     4981 gtatatatat atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta
     5041 tatgcgtcta tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt
     5101 ccatgcgggg tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca
     5161 ctcctcaatt ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata
     5221 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     5281 tcgtc
//
