LOCUS       Exported                6166 bp ds-DNA     circular SYN 21-NOV-2016
DEFINITION  Cloning vector pESC-LEU, complete sequence.
ACCESSION   AF063849
VERSION     .
KEYWORDS    73295_Genbank
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Lu Q.
  TITLE     pESC-LEU
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 6166)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 21, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     ApEinfo:methylated:1
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          131..1340
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..20
                     /note="TJOS-101R"
     misc_feature    29..48
                     /note="TJOS-101F"
     misc_feature    99..118
                     /note="TJOS-102R"
     misc_signal     131..1340
                     /gene="(null)"
                     /note="(null)"
     misc_feature    131..517
                     /gene="TEF1p"
                     /product="TEF1 promoter of A. gossypii"
                     /note="TEF1 promoter of A. gossypii"
     CDS             519..1091
                     /codon_start=1
                     /gene="nat"
                     /product="Nourseothricin acetyltransferase"
                     /note="Nourseothricin acetyltransferase"
                     /note="nat"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTTTGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     misc_feature    1092..1340
                     /gene="TEF1t"
                     /product="TEF1 terminator of A. gossypii"
                     /note="TEF1 terminator of A. gossypii"
     misc_feature    1349..1368
                     /note="TJOS-102F"
     misc_feature    1419..1438
                     /note="TJOS-97R"
     misc_feature    1433..1438
                     /note="PvuII"
     promoter        1472..1740
                     /note="SNR52 Promoter"
     misc_feature    1761..1839
                     /note="Structural Guiding RNA"
     misc_feature    1840..1859
                     /note="SUP4 Terminator"
     promoter        1909..2176
                     /note="SNR52 Promoter"
     misc_feature    2197..2275
                     /note="Structural Guiding RNA"
     misc_feature    2276..2295
                     /note="SUP4 Terminator"
     promoter        2345..2613
                     /note="SNR52 Promoter"
     misc_feature    2634..2712
                     /note="Structural Guiding RNA"
     misc_feature    2713..2732
                     /note="SUP 4 Terminator"
     misc_feature    2766..2785
                     /note="TJOS-97F"
     misc_feature    2766..2771
                     /note="PvuII(1)"
     rep_origin      2949..3616
                     /direction=RIGHT
                     /note="pUC ori"
     CDS             complement(3767..4624)
                     /codon_start=1
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4758..5913
                     /direction=RIGHT
                     /note="2 micron ori"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggct
      121 gcatttaaat gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
      181 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
      241 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
      301 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
      361 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
      421 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
      481 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaccact cttgacgaca
      541 cggcttaccg gtaccgcacc agtgtcccgg gggacgccga ggccatcgag gcactggatg
      601 ggtccttcac caccgacacc gttttccgcg tcaccaccac cggggacggc ttcaccctgc
      661 gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa tcggacgacg
      721 aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg tacggggacg
      781 acggcgacct ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg
      841 tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg
      901 ggctcgcgac ggagttcgcc cgcgagcggg gcgccgggca cctctggctg gaggtcacca
      961 acgtcaacgc accggcgatc cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg
     1021 acaccgccct gtacgacggc accgcctcgg acggcgagca ggcgctctac atgagcatgc
     1081 cctgccccta atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt
     1141 atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt
     1201 ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg
     1261 cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca
     1321 tccagtgtcg aaaacgagct gcggccgcct acagggcgcg tcgcgccatt cgccattcag
     1381 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctgga
     1441 atgcgtgcga tagggaacaa aagctggagc ttctttgaaa agataatgta tgattatgct
     1501 ttcactcata tttatacaga aacttgatgt tttctttcga gtatatacaa ggtgattaca
     1561 tgtacgtttg aagtacaact ctagattttg tagtgccctc ttgggctagc ggtaaaggtg
     1621 cgcatttttt cacaccctac aatgttctgt tcaaaagatt ttggtcaaac gctgtagaag
     1681 tgaaagttgg tgcgcatgtt tcggcgttcg aaacttctcc gcagtgaaag ataaatgatc
     1741 ctctcgaagt ggtcacgtgc gttttagagc tagaaatagc aagttaaaat aaggctagtc
     1801 cgttatcaac ttgaaaaagt ggcaccgagt cggtggtgct ttttttgttt tttatgtctt
     1861 cgagtcatgt aattagttaa gtgcaggtag ggaacaaaag ctggagcttc tttgaaaaga
     1921 taatgtatga ttatgctttc actcatattt atacagaaac ttgatgtttt ctttagagta
     1981 tatacaaggt gattacatgt acgtttgaag tacaactcta gattttgtag tgccctcttg
     2041 ggctagcggt aaaggtgcgc attttttcac accctacaat gttctgttca aaagattttg
     2101 gtcaaacgct gtagaagtaa agttggtgcg catgtttcgg cgttcgaaac ttctccgcag
     2161 tgaaagataa atgatctgag aatactgttg taaaacgttt tagagctaga aatagcaagt
     2221 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt ggtgcttttt
     2281 ttgtttttta tgtcttcgag tcatgtaatt agttaatctg tcatagggaa caaaagctgg
     2341 agcttctttg aaaagataat gtatgattat gctttcactc atatttatac agaaacttga
     2401 tgttttcttt cgagtatata caaggtgatt acatgtacgt ttgaagtaca actctagatt
     2461 ttgtagtgcc ctcttgggct agcggtaaat gtgcgcattt tttcacaccc tacaatgttc
     2521 tgttcaaaag attttggtca aacgctgtag aagtgaaagt tggtgcgcat gtttcggcgt
     2581 tcgaaacttc tccgcagtga aagataaatg atcgcttcaa gaattgagta aacgttttag
     2641 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     2701 agtcggtggt gctttttttg ttttttatgt cttcgagtca tgtaattagt taatcgcgtg
     2761 cattccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2821 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2881 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2941 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3001 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3061 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3121 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3181 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3241 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3301 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3361 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3421 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     3481 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3541 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3601 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3661 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3721 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3781 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3841 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3901 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3961 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4021 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4081 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4141 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4201 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4261 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4321 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4381 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4441 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4501 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4561 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4621 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4681 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4741 gaaaagtgcc acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg
     4801 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
     4861 gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca
     4921 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
     4981 gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa
     5041 atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt
     5101 gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga
     5161 agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc
     5221 gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt
     5281 atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga
     5341 ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta
     5401 taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca
     5461 atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta
     5521 gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga
     5581 tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag
     5641 tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt
     5701 ggttttcaaa agcgctctga agttcctata ctttctagag aataggaact tcggaatagg
     5761 aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata
     5821 cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag
     5881 aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag
     5941 gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg
     6001 cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc
     6061 tcatccttca atgctatcat ttcctttgat attggatcat actaagaaac cattattatc
     6121 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
