LOCUS       pICPIS-CB        3992 bp    DNA     circular      7-JAN-2016
DEFINITION  Complementary copy of pL-ICPI.
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11052887|
COMMENT     VNTDBDATE|-11052887|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pICPIS-CB|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pICPIS-CB" 0 3992 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatII" "AscI" "BamHI" "ClaI" "EcoRI" "HindIII" "MfeI" 
COMMENT         "MluI" "NcoI" "NdeI" "NheI" "NotI" "PacI" "PstI" "PvuI" "SalI" "ScaI" 
COMMENT         "SmaI" "SwaI" "XbaI" "XhoI" "XmaI" "ApaI" "PmeI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3992 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AatII" "gacgtc" 6 0 3 3018 0 3071 0 3154 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 2 2896 0 3972 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 3746 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 3786 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3778 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "MfeI" "caattg" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "MluI" "acgcgt" 2 0 2 2937 0 3919 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 3127 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 3752 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3767 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "PstI" "ctgcag" 6 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 142 0 1238 0 3550 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 1126 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "SwaI" "atttaaat" 5 0 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 3233 0 3773 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 3983 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 1 3761 0 0 "")) 
COMMENT       (CObList 
COMMENT        #24=(CFSignal (CObList) "I-CeuI" 21 0 0 2902 2927 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2902..2927") 
COMMENT        #25=(CFSignal (CObList) "PI-SceI" 21 0 0 3931 3969 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3932..3970") 
COMMENT        #26=(CFSignal (CObList) "ampicillin resistance (ORF)" 21 0 0 819 1676 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "819..1676") 
COMMENT        #27=(CFSignal (CObList) "pUC origin" 25 0 0 1827 2494 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1827..2494") 
COMMENT        #28=(CFSignal (CObList) "lac promoter" 21 0 0 2714 2835 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2714..2835") 
COMMENT        #29=(CFSignal (CObList) "f1 origin" 21 0 0 198 504 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "198..504") 
COMMENT        #30=(CFSignal (CObList) "chicken beta actin promoter" 29 0 0 3239 3511 0 
COMMENT             (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#800000" 
COMMENT              "/ApEinfo_revcolor=#800000") 1 1 1 1 "3240..3512") 
COMMENT        #31=(CFSignal (CObList) "CMV promoter" 9 0 0 2954 3238 0 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=cyan" "/ApEinfo_revcolor=#80ffff") 
COMMENT             1 1 1 1 "2955..3239") 
COMMENT        #32=(CFSignal (CObList) "SV40 polyA" 21 0 0 3791 3917 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3792..3918") 
COMMENT        #33=(CFSignal (CObList) "M13 Foward Primer (-40)" 21 0 1 44 60 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #34=(CFSignal (CObList) "M13 Reverse Primer" 21 0 0 2829 2844 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
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COMMENT                   "" 0 4) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
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COMMENT                          "DNA 'pICPIS-CB'" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Complementary copy of pL-ICPI." 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 01/07/16 01:02PM" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/07/16 01:02PM" 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 3992 bp" 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 4-DEC-2013" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #49=(CRefLinePar 
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COMMENT                   0 0) 
COMMENT              #50=(CFolderPar 
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COMMENT                   "Annotations") 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2955..3239" 1) 
COMMENT                                   #57=(CLinePar 
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COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /ApEinfo_fwdcolor=cyan" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /ApEinfo_revcolor=#80ffff" 1))) 
COMMENT                                 "CMV promoter"))) "Enhancer (1 total)") 
COMMENT                     #60=(CFolderPar 
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COMMENT                                        "Start: 44    End: 60   (Complementary)" 
COMMENT                                        1))) "M13 Foward Primer (-40)") 
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COMMENT                                 "ampicillin resistance (ORF)") 
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COMMENT                                   #73=(CLinePar 
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COMMENT                                   #74=(CLinePar 
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COMMENT                                        "   2714..2835" 1))) "lac promoter") 
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COMMENT                                 "M13 Reverse Primer") 
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COMMENT                                   #79=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2902..2927" 1))) "I-CeuI") 
COMMENT                            #81=(CFolderPar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
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COMMENT                                        "   3792..3918" 1))) "SV40 polyA") 
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COMMENT                                   #88=(CLinePar 
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COMMENT                          "Misc. Feature (8 total)") 
COMMENT                     #89=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #90=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                        "Start: 1827  End: 2494" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1827..2494" 1))) "pUC origin"))) 
COMMENT                          "PolyA Signal (1 total)") 
COMMENT                     #94=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #95=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                        "Start: 3239  End: 3511" 1) 
COMMENT                                   #97=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #98=(CLinePar 
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COMMENT                                        "   3240..3512" 1) 
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COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   /ApEinfo_fwdcolor=#800000" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   /ApEinfo_revcolor=#800000" 1))) 
COMMENT                                 "chicken beta actin promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)"))) "Feature Map") 
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COMMENT                     (CObjectList 
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COMMENT                                            " N2: 3071 " 1) 
COMMENT                                      #107=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3154 " 1))))) "AatII: 3 sites") 
COMMENT                            5 1 "GACGTC" "CTGCAG") 
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COMMENT                            2 6 "GGCGCGCC" "CCGCGCGG") 
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COMMENT                                            " N1: 3786 " 1))))) "ClaI: 1 site") 
COMMENT                            2 4 "ATCGAT" "TAGCTA") 
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COMMENT                            "TTCGAA") 
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COMMENT                            1 5 "ACGCGT" "TGCGCA") 
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COMMENT                            "GGTACC") 
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COMMENT                            2 4 "CATATG" "GTATAC") 
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COMMENT                            1 5 "GCTAGC" "CGATCG") 
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COMMENT                              (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" 
COMMENT                            "AATTAATT") 
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FEATURES             Location/Qualifiers
     misc_feature    2902..2927
                     /vntifkey="21"
                     /label=I-CeuI
     misc_feature    3931..3969
                     /vntifkey="21"
                     /label=PI-SceI
     misc_feature    819..1676
                     /vntifkey="21"
                     /label=ampicillin\resistance\(ORF)
     polyA_signal    1827..2494
                     /vntifkey="25"
                     /label=pUC\origin
     misc_feature    2714..2835
                     /vntifkey="21"
                     /label=lac\promoter
     misc_feature    198..504
                     /vntifkey="21"
                     /label=f1\origin
     promoter        3239..3511
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=#800000
                     /vntifkey="29"
                     /label=chicken\beta\actin\promoter
     enhancer        2954..3238
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#80ffff
                     /vntifkey="9"
                     /label=CMV\promoter
     misc_feature    3791..3917
                     /vntifkey="21"
                     /label=SV40\polyA
     misc_feature    complement(44..60)
                     /vntifkey="21"
                     /label=M13\Foward\Primer\(-40)
     misc_feature    2829..2844
                     /vntifkey="21"
                     /label=M13\Reverse\Primer
BASE COUNT      951 a      1006 c      1024 g      1011 t 
ORIGIN
        1 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac 
       61 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 
      121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 
      181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
      241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
      301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 
      361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
      421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
      481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
      541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
      601 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac 
      661 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa 
      721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 
      781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 
      841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 
      901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 
      961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 
     1021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 
     1081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 
     1141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 
     1201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 
     1261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 
     1321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 
     1381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 
     1441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 
     1501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 
     1561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 
     1621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 
     1681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 
     1741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 
     1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 
     1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 
     1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 
     1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 
     2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 
     2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 
     2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 
     2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 
     2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 
     2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 
     2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 
     2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 
     2521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 
     2581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 
     2641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 
     2701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 
     2761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 
     2821 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac 
     2881 tcaagctatg catggcgcgc ctaactataa cggtcctaag gtagcgacgc gccgaacgcg 
     2941 tggtacctct ggtcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
     3001 accccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 
     3061 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 
     3121 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 
     3181 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct actcgaggcc 
     3241 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt 
     3301 attttttaat tattttgtgc agcgatgggg gcgggggggg gggggggggg ggcgcgcgcc 
     3361 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc 
     3421 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc 
     3481 ctataaaaag cgaagcgcgc ggcgggcggg agcgggatca gccaccgcgg tggcggccct 
     3541 agagtcgatc gaggaactga aaaaccagaa agttaactgg taagtttagt ctttttgtct 
     3601 tttatttcag gtccccgatc cggtggtggt gcaaatcaaa gaactgctcc tcagtggatg 
     3661 ttgcctttac ttctaggcct gtacggaagt gttacttctg ctctaaaagc tgcggaattg 
     3721 tacccgcggc cgatccaccg gtcgggatcc gctagcgttt aaacgcggcc gctcgagaat 
     3781 tctatcgata agtaagtgac tagaacttgt ttattgcagc ttataatggt tacaaataaa 
     3841 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     3901 tgtccaaact catctgcacg cgtggcgcgt atctatgtcg ggtgcggaga aagaggtaat 
     3961 gaaatggcag gcgcgccggg cccaattcgc cc 
//
