LOCUS       P76_LasI900             3877 bp ds-DNA     linear       15-DEC-2015
DEFINITION  pBR322 converted to BioBrick vector (formally BBa_I739201)
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    65..97
                     /label=RBS900
                     /ApEinfo_fwdcolor=#fff424
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            1133..1259
                     /gene="from pSC101"
                     /label=from pSC101
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1412..1603
                     /gene="rop"
                     /label=rop
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            1508..1664
                     /gene="H-strand Y effector site"
                     /label=H-strand Y effector site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(1848..1911)
                     /gene="L-strand Y effector site"
                     /label=L-strand Y effector site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(2016..2630)
                     /gene="rep pMB1"
                     /label=rep pMB1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(2031..2583)
                     /gene="RNAII transcript"
                     /label=RNAII transcript
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2437..2442
                     /label=RNAI transcript promoter -35 sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2459..2464
                     /label=RNAI transcript promoter -10 sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            2473..2580
                     /gene="RNAI transcript"
                     /label=RNAI transcript
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1..55
                     /label=pLux76
                     /ApEinfo_fwdcolor=#ff1515
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(2593..2598)
                     /label=RNAII transcript promoter -10 sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(2614..2619)
                     /label=RNAII transcript promoter -35 sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            2645..3858
                     /gene="Repeat region 1"
                     /label=Repeat region 1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2790..3650)
                     /gene="beta-lactamase (bla gene)"
                     /label=beta-lactamase (bla gene)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2793..3581)
                     /gene="beta-lactamase mature peptide"
                     /label=beta-lactamase mature peptide
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3582..3650)
                     /gene="beta-lactamase signal peptide"
                     /label=beta-lactamase signal peptide
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     RBS             complement(3658..3662)
                     /label=bla gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(3694..3699)
                     /label=bla(P3) promoter -10 sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(3715..3720)
                     /label=bla(P3) promoter -35 sequence 
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3856..3877
                     /label=DNA: BioBrick prefix
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    713..773
                     /label=Voigt terminator L3S2P21
                     /ApEinfo_fwdcolor=#ff2126
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    104..712
                     /label=Bba_C0178 LasI
                     /ApEinfo_fwdcolor=#ff23f0
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ACCTGTAGGA TCGTACAaGT TTACGCAAGA AAATGGTTTG TTATAGTCGA ATAAAatact
       61 agagCGCACG CTTAACCAAG TAAAAGGGTT CGTTTTTtac tagATGATtg ttcagattgg
      121 ccgtcgtgag gaatttgata aaaagctgct gggcgagatg cacaaactgc gtgcacaggt
      181 tttcaaggag cgtaaaggtt gggacgttag cgttattgac gaaatggaga ttgatggcta
      241 cgacgctctg tctccgtatt atatgctgat tcaggaggac accccggaag ctcaggtatt
      301 tggttgctgg cgtatcttcg atactacggg tccgtatatg ctgaaaaata ccttcccgga
      361 actgctgcac ggtaaagaag cgccttgtag cccgcacatc tgggaactgt ctcgtttcgc
      421 tatcaactcc ggccagaaag gttccctggg cttttccgat tgtaccctgg aagcaatgcg
      481 tgcgctggcg cgctattccc tgcaaaacga catccagact ctggtgaccg ttactactgt
      541 gggcgttgaa aagatgatga tccgtgcagg cctggacgtt agccgtttcg gtccacacct
      601 gaagatcggt atcgaacgcg cggtggccct gcgtatcgaa ctgaatgcaa aaacccagat
      661 cgcactgtat ggtggtgttc tggtggaaca acgcctggcc gtttcctaat aaCTCGGTAC
      721 CAAATTCCAG AAAAGAGGCC TCCCGAAAGG GGGGCCTTTT TTCGTTTTGG TCCatggaag
      781 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg
      841 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc
      901 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat
      961 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
     1021 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
     1081 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
     1141 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg
     1201 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
     1261 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
     1321 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat
     1381 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa
     1441 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
     1501 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
     1561 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     1621 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     1681 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     1741 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     1801 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     1861 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1921 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1981 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2041 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2101 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2161 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2221 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2281 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2341 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2401 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2461 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2521 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2581 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2641 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2701 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2761 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2821 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2881 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2941 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3001 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3061 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgtagg catcgtggtg
     3121 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3181 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3241 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3301 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3361 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     3421 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3481 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3541 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3601 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3661 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3721 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3781 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     3841 ccctttcgtc ttcaagaatt cgcggccgct tctagag  
//
