LOCUS       P81_LuxI_32             3833 bp ds-DNA     linear       15-DEC-2015
DEFINITION  pBR322 converted to BioBrick vector (formally BBa_I739201)
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc            complement(968..972)
                     /gene="gamma-delta insertion sequence"
                     /label=gamma-delta insertion sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            1089..1215
                     /gene="from pSC101"
                     /label=from pSC101
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1368..1559
                     /gene="rop"
                     /label=rop
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             84..668
                     /gene="LuxI"
                     /label=BBa_C0161 LuxI
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            1464..1620
                     /gene="H-strand Y effector site"
                     /label=H-strand Y effector site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(1804..1867)
                     /gene="L-strand Y effector site"
                     /label=L-strand Y effector site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(1972..2586)
                     /gene="rep pMB1"
                     /label=rep pMB1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(1987..2539)
                     /gene="RNAII transcript"
                     /label=RNAII transcript
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2393..2398
                     /label=RNAI transcript promoter -35 sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2415..2420
                     /label=RNAI transcript promoter -10 sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            2429..2536
                     /gene="RNAI transcript"
                     /label=RNAI transcript
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(2549..2554)
                     /label=RNAII transcript promoter -10 sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(2570..2575)
                     /label=RNAII transcript promoter -35 sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            2601..3814
                     /gene="Repeat region 1"
                     /label=Repeat region 1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2746..3606)
                     /gene="beta-lactamase (bla gene)"
                     /label=beta-lactamase (bla gene)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2749..3537)
                     /gene="beta-lactamase mature peptide"
                     /label=beta-lactamase mature peptide
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3538..3606)
                     /gene="beta-lactamase signal peptide"
                     /label=beta-lactamase signal peptide
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     RBS             complement(3614..3618)
                     /label=bla gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(3650..3655)
                     /label=bla(P3) promoter -10 sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(3671..3676)
                     /label=bla(P3) promoter -35 sequence 
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3812..3833
                     /label=DNA: BioBrick prefix
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    669..729
                     /label=Voigt terminator L3S2P21
                     /ApEinfo_fwdcolor=#ff2126
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    65..77
                     /label=RBS B0032
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1..55
                     /label=pLas81
                     /ApEinfo_fwdcolor=#ff0c0e
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ACCTaTAGGA TCGTAtAGGT TTACGCAAGA AAATGGTTTG TTATAGTCGA ATAAAatact
       61 agagtcacac aggaaagtac tagatgacta taatgataaa aaaatcggat tttttggcaa
      121 ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac
      181 ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat aactcaaatg
      241 cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg cgtttattac
      301 ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt caacagagtg
      361 ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa
      421 agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata tataaacacg
      481 ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata gagcgatttt
      541 taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat gtattaggtg
      601 atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa
      661 attaataaCT CGGTACCAAA TTCCAGAAAA GAGGCCTCCC GAAAGGGGGG CCTTTTTTCG
      721 TTTTGGTCCa tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag
      781 ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca
      841 tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc
      901 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
      961 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
     1021 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
     1081 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
     1141 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
     1201 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
     1261 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
     1321 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
     1381 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
     1441 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
     1501 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1561 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1621 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     1681 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     1741 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     1801 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1861 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1921 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1981 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2041 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2101 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2161 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2221 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2281 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2341 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2401 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2461 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2521 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2581 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2641 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2701 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2761 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2821 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2881 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2941 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3001 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3061 tgtaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3121 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3181 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3241 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3301 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3361 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3421 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3481 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3541 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3601 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3661 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3721 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     3781 taggcgtatc acgaggccct ttcgtcttca agaattcgcg gccgcttcta gag
//
