LOCUS       J07\pCL-V001-pAAV-HDR\Fah2\template        4989 bp    DNA     circular      9-APR-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|730709073|
COMMENT     LSOWNER|
COMMENT     VNTNAME|J07 pCL-V001-pAAV-HDR Fah2 template|
COMMENT     VNTAUTHORNAME|mit|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /dnas_title="L-ITR"
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    2252..2392
                     /dnas_title="R-ITR"
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2484..2790
                     /dnas_title="f1 Origin"
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3309..4166
                     /dnas_title="AmpR"
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    4024..4029
                     /vntifkey="21"
                     /label=Misc_Feature_2
                     /note="BsaI"
     rep_origin      4317..4984
                     /dnas_title="pUC ori"
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    148..153
                     /vntifkey="21"
                     /label=Misc_Feature_3
                     /note="XbaI"
     source          1..4989
                     /dnas_title="pCL-V001-pAAV-U6-BbsIBB"
                     /vntifkey="98"
     primer          2453..2472
                     /vntifkey="27"
                     /label=AAVSeqF
     primer_bind     4977..7
                     /vntifkey="28"
                     /label=AAVSeqR
     misc_feature    154..579
                     /vntifkey="21"
                     /label=U6\sgRNA
     misc_feature    580..1399
                     /vntifkey="21"
                     /label=5'\arm-genomic\PCR
     CDS             1400..2243
                     /vntifkey="4"
                     /label=3'\arm-gblock
     misc_feature    1417..1417
                     /vntifkey="21"
                     /label=A->G\repair
     mRNA            403..423
                     /vntifkey="54"
                     /label=sgFah
     misc_feature    1426..1428
                     /vntifkey="21"
                     /label=mutate\PAM2
BASE COUNT     1194 a      1233 c      1248 g      1314 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggcctct agagagggcc tatttcccat gattccttca 
      181 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac 
      241 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca 
      301 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc 
      361 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgacgactg gagcagtaat 
      421 gccgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa 
      481 agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt aaaataaggc 
      541 tagtccgttt ttagcgcgtg cgccaattct gcagacaaaa agacccacac taaggctcca 
      601 ctctttattt aaattaagca ctttattttg agagtgctga agactcatat gtagttgtga 
      661 gaaatgaggt ggagatcccc tgaatttgaa agagatgggg tccttcatgc ctcggtgccc 
      721 tcagctctct ctctctctct ctctctctct ctctctctct gtgtgtgtgt gtgtgtgtgt 
      781 gtgtgtgtat cttgaagatt ttgcactgtt taaaccctat ctcaaggaca aataaaacag 
      841 tgaccctagc catgaacgta gatgattctg tactgctcaa ggaattcctt ctcccccgca 
      901 cttagtttcc cttagacatg attgctagat gttctcttgg tccatggcta ttggacagat 
      961 gccattcctt ttgggatagg tagggctgtg aagtcaactt gtcaatcctc ctggaatgag 
     1021 cacccctgat gttttcattc tttacaactg tcatatgctg tcagatgtga gggtatggag 
     1081 cctggaatac agaggaagga tcagatcaga aaggcctggg gtgccctggg gctggggggg 
     1141 ggggaaaggg ctcatgtgag tagatttccc accttagaga cccagggaag taatgccagg 
     1201 tcctcaggca gccctagtcc ctggttgaac tttgaaaata ttttcccttt gctctgtaag 
     1261 ccacagtgac ccagagcatc gggtcatcta gattcttacc aactttctcc atggcaggct 
     1321 ttcttcgtag gccctgggaa cagattcgga gagccaatcc ccatttccaa agcccatgaa 
     1381 cacattttcg ggatggtcct catgaacgac tggagcggta atgcctcctg gcccagcttc 
     1441 ctctgatgtt ctgttcttag gggcacacac aggagttggg tatgggacag gaggcctaag 
     1501 tactacaggg gtgataccat gcagacttct gactctgtgg gtgtggggca gtcacagctt 
     1561 ccctgagtag ctttctcata agtggaagga tggagctgac agaacctaaa gctttatcaa 
     1621 gccctacaca ctccactcac tgttgccagc accatcagct gctgatgaag agctgtacat 
     1681 ggagggattt tagtcagtgg ctgaattgag ccttactatt catcagccca gcatctgagt 
     1741 cccagctcta caccctgata ccatgtgtaa gaggaagtgg gtccagaaat ctcaggagag 
     1801 ggccttcccc cattcaggcc cttggtaggg actgtcctgt catccttcat tgttccctag 
     1861 cacagcacct ccaaggacct ctgtcttcat actgaatacc tttctgtttg ttccctggga 
     1921 gatctggttg gccagagcca tgtagagagg tccatgtagg tcaaggacag gaagcagctt 
     1981 ggagtgagtc tgtaagccac ggtgagacct aagcttgctg tcttttcata gcacgagaca 
     2041 tccagcaatg ggagtacgtc ccacttgggc cattcctggg gaaaagcttt ggaaccacaa 
     2101 tctccccgtg ggtggtgcct atggatgccc tcatgccctt tgtggtgcca aacccaaagc 
     2161 aggtaagcac ccttctgctg aaaactctgc tctaagttcc ggcttccgcc ctttcctgtt 
     2221 gccctctgat actgagacat ggtgcggccg caggaacccc tagtgatgga gttggccact 
     2281 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg 
     2341 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg 
     2401 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac 
     2461 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 
     2521 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 
     2581 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 
     2641 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta 
     2701 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 
     2761 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg 
     2821 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 
     2881 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta 
     2941 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 
     3001 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 
     3061 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc 
     3121 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 
     3181 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     3241 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     3301 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     3361 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     3421 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     3481 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     3541 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     3601 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     3661 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     3721 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     3781 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     3841 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     3901 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     3961 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     4021 gtggaagccg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     4081 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     4141 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     4201 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     4261 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     4321 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     4381 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     4441 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 
     4501 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     4561 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     4621 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     4681 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     4741 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     4801 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     4861 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     4921 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     4981 ctcacatgt 
//
