LOCUS       pMLS235         6538 bp ds-DNA     circular     03-FEB-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1601..2214
                     /label=GFP w/ syntrons
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1568..1600
                     /label=Flexible Linker
                     /ApEinfo_fwdcolor=#a5a9a4
                     /ApEinfo_revcolor=#a5a9a4
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2215..2248
                     /label=LoxP
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1401..1418
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1769..1819
                     /label=syntron1
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2189..2214
                     /label=syntron3
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1974..2024
                     /label=syntron2
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4406..4655
                     /label=GFP w/ syntrons(1)
                     /ApEinfo_label=GFP w/ syntrons
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     join(1435..1480,1482..1516)
                     /label=attP1
                     /ApEinfo_fwdcolor=plum
                     /ApEinfo_revcolor=plum
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4406..4430
                     /label=syntron3(1)
                     /ApEinfo_label=syntron3
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        142..640
                     /label=U6 promoter
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4372..4405
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    668..750
                     /label=sgRNA scaffold
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(2254..4371)
                     /label=C.Briggsae unc-119(+)
                     /ApEinfo_fwdcolor=#b8b5ff
                     /ApEinfo_revcolor=#b8b5ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(751..987)
                     /label=U6 downstream
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2538..3383)
                     /label=CB-unc-119(+)
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    641..667
                     /label=SapI Insertion Site
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3073..3115)
                     /label=Intron
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2645..2695)
                     /label=Intron(1)
                     /ApEinfo_label=Intron
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1539..1564
                     /label=SapI Cloning Site
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3232..3326)
                     /label=Intron(2)
                     /ApEinfo_label=Intron
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(4825..4845)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(4805..4826)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      5834..6520
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             4958..5764
                     /label=KanR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(4710..4787)
                     /label=attP2
                     /ApEinfo_fwdcolor=plum
                     /ApEinfo_revcolor=plum
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4659..4684
                     /label=SapI Cloning Site(1)
                     /ApEinfo_label=SapI Cloning Site
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
       61 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
      121 GAGTCAGTGA GCGAGGAAGC GTCCAAGAAC TCGTACAAAA ATGCTCTGAA GTAGGTCTCG
      181 AGATCAATAC TGGGAAGACG AAAGTCTTGC GAAACCGATT CGCTGACCCC AGTAAAGTCT
      241 ACTTCGGTAG CCCTTCCCCC ACCACCCAGC TCGACGACGT CGACGAGTAC ATCTACCTCG
      301 GTCGTCAAAT CAACGCCCAA AACAACTTGA TGCCGGAAAT CCACCGAAGA CGTCGAGCAG
      361 CCTGGGCTGC ATTCAATGGA ATCAAGAATG CCACCGACTC CATCACCGAC AAGAAGATTC
      421 GTGCGAATCT GTTCGACTCA ATTGTCCTTC CAGCGCTCAC CTACGTTTCA GAAGCCTGGA
      481 CATTCACCAA AGCTCTATCC GAACGAGTAC GAATCACACA TGCCTCCCCC ACAAATTATT
      541 GATCTACATC CCCATTTGGT ATAGTGTCTT ACAGGACTCT CAATATGTCA GCTGCATGAT
      601 AAATGGTCCC TATATAATTG AATTGCAAAT CTAAATGTTT GCGAAGAGCg gatccatggG
      661 CTCTTCGGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG
      721 AAAAAGTGGC ACCGAGTCGG TGCTTTTTTT CAATAATATT CTAGATATCC CTTTTTGAAA
      781 CTAAAATGAA ATACTCTAAA TTTTTGAGTT CAATGTTTCA ACTAATCTTG GTTAAAGGTA
      841 AATTCCCGGA GGGACGATTT TAAAGTTGCC AGAGTTGATT AAATTGGTCC AAAAAATTTT
      901 ATTAGGAAAC TCGAAAAAAA ATTGATTTTT TTTGACAATA TTCCAAAGAA AATTACAAAA
      961 TCTCGACGCC AAACATAGTC GGCTGTGGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
     1021 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC
     1081 GCAACGCAAT TAATACGCGT ACCGCTAGCC AGGAAGAGTT TGTAGAAACG CAAAAAGGCC
     1141 ATCCGTCAGG ATGGCCTTCT GCTTAGTTTG ATGCCTGGCA GTTTATGGCG GGCGTCCTGC
     1201 CCGCCACCCT CCGGGCCGTT GCTTCACAAC GTTCAAATCC GCTCCCGGCG GATTTGTCCT
     1261 ACTCAGGAGA GCGTTCACCG ACAAACAACA GATAAAACGA AAGGCCCAGT CTTCCGACTG
     1321 AGCCTTTCGT TTTATTTGAT GCCTGGCAGT TCCCTACTCT CGCGTTAACG CTAGCATGGA
     1381 TGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTCT TAAGCTCGGG CCCCAAATAA
     1441 TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATGAG CAATGCTTTT
     1501 TTATAATGCC AACTTTgtac aaaaaagcag gctcgCCGCG AAGAGCggat ccatggGCTC
     1561 TTCGGGCAGC GGTGGCAGTG GAGGTACCGG CGGAAGCGGT AGCAAAGGAG AAGAACTTTT
     1621 CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC ACAAATTTTC
     1681 TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA AATTTATTTG
     1741 CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac atatatatac taactaaccc
     1801 tgattattta aattttcagC CAACACTTGT CACTACTTTC TGTTATGGTG TTCAATGCTT
     1861 CTCGAGATAC CCAGATCATA TGAAACGGCA TGACTTTTTC AAGAGTGCCA TGCCCGAAGG
     1921 TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG AACTACAAGA CACgtaagtt
     1981 taaacagttc ggtactaact aaccatacat atttaaattt tcagGTGCTG AAGTCAAGTT
     2041 TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG
     2101 AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC
     2161 AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac atgattttac taacATAACT
     2221 TCGTATAATG TATGCTATAC GAAGTTATtt ctagacattc tctaatgaaa aaatctttca
     2281 gttgaaattg aaaatgagtt aaagttggag tttttattga aaacagattt ccgtgtgatt
     2341 agtgttttta gcgagtgtga caggacagcg aaaaaatata gaaacaaggg gggaactgaa
     2401 aagcttagga atgcattgaa catgagaagg ggaaggggaa ggaacaaact agacaggaat
     2461 tattggaatt taatcacatt tggagttttt tttctattcg acagaataat tatccagaac
     2521 atttttgtat taaatatTTA TGCATCATAT GAGTAGTCGG CTTTGTTGTG CATGACGAGT
     2581 TTGTTATCGA CGAAATAGAA GCTGTCAGAA CGAGTCTCGT TTGGATTGTT GATCATGTCG
     2641 TCCActgaaa aagagattag tcttttgaat tgtacttttt agaataatga ctcacTGAGT
     2701 TGTTGAGAGA GTTGAGGGAA CTCATAGATA TGTTCACAGT TGTTTCGTGA ATTCGGAATA
     2761 CAGAATCCGA ATTCAAAGTC AAAACACTTC AGAAGGCGAT CTTTGAAGAA GTGACGTTCG
     2821 ATCATTCGGA AATGATGGAT TGGGATGTCT CCTACTTTGA ATTCCACAGT TGCTCCGACC
     2881 GTTTTGAGTT TCAAAAAGTT TGGAGCAAAT CTATAACGCA CGTATCTTGC CGACTCCTGT
     2941 GGCGACTCAT CATTCTCCTG ATCGTTCTCC GGTTTGGCGA TCTCGAAAAG CACTTGCTCA
     3001 GTGTCCAGAT CACGGATTTG GAACTTGGTG AACTCGATGT TATAGATGTT CGCAGATGGG
     3061 GAGCATAAGA ATcctaaatt tatgttttaa actgaaatcc aaagggagca agataCCTTG
     3121 AGTGATTCCC GGAAGTGCTA AAACGTCGTT CGGAGTGATT TGAGCTTTCT TCGCAAGCTC
     3181 CGATTCCGTT GTGATTCCTT GTTCGGTGCT TGGTGGTGGC CGTGGCATCT Ggaaatatgg
     3241 aaaagttcaa caaaaagaaa agagaaaaga atgaaatcgg atatcaagag ttagttgagc
     3301 ggtttctcta gttttctgag tctcacCTGC GACGGGAAGG TCGCCGAGCC GGGTGGAATC
     3361 GATTGTTGTT GCTCGGCTTT CATatcggtt tggttggaag cggctgaaaa cggaaagaag
     3421 tggaagaagg aaaagagtgt ggtgtgacag gaaaatggta attagagggt gccaaataac
     3481 cagctatatt ttgttttttt ttgaaaacat ttttaaaaag aaaaatacga taatgatatc
     3541 agatggattt ccggaaaact ggtatgaaaa atttcaacct ttttgagtac atgtaatcaa
     3601 aatacacttt gtaaattatc atttttattg aaactccacc atttttctat ttataacgct
     3661 aataatttga aaaagaaacc tgttgcgaac cgcggggtga atcccaaaaa cgaatgcgtt
     3721 ttggtggagt gattgattcg aatcgaagaa gaaaaagaag aagacgtgga atagagagct
     3781 cactcttaac cgagcagcac acaccgacag aaaaaaaaat gaaatgaatg agggtcttct
     3841 tcttcttctt cttcgaatga ttgacagaaa tgggaaaaag aggaagattg agaagggaaa
     3901 aaggaaggag aaaagaagca gaagaagacg tcagagagga gaggaacgag cggaaaagca
     3961 gcgggcgcaa gtcatagaag tagcagagct ggggagaaga agacactatc caagaaagga
     4021 atgacgagag agtatgcaaa ggggtatagg gtgcagacag aataggaaca gaataacaga
     4081 tgatgagcca agaagagttg aaaagggcga tgaatttgtc atgtaactta atttgggtca
     4141 atttgagcat gatgaattga aatcatccct tgttgggagt taataaccgg tttgttatca
     4201 gaaaccctgt aatagaaggg cgccctaact ttgagccaat tcatcccggt ttctgtcaaa
     4261 tatatcaaaa agtggtcaac tgacaaattg tttttgatat tataataaac attttatccg
     4321 ttaacaattt tcgaatactt tttacaagga cttggataaa ttggctcaaa gATAACTTCG
     4381 TATAATGTAT GCTATACGAA GTTATtaact aatctgattt aaattttcag AACTTCAAAA
     4441 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC
     4501 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC
     4561 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG
     4621 CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATGACG AAGAGCggat ccatggGCTC
     4681 TTCGACTgac ccagctttCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT
     4741 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA
     4801 TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT
     4861 GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAATA ATATCATCAT GAACAATAAA
     4921 ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC
     4981 GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG
     5041 CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC
     5101 AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT
     5161 CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC
     5221 TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT
     5281 AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG
     5341 GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC
     5401 TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG
     5461 TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC
     5521 GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA
     5581 ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC
     5641 CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA
     5701 ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT
     5761 TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG
     5821 GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC
     5881 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT
     5941 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA
     6001 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
     6061 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA
     6121 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC
     6181 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG
     6241 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG
     6301 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
     6361 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT
     6421 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
     6481 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCC
//