LOCUS       pMLS252                 5621 bp ds-DNA     circular     03-FEB-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Insert from GFP PCR 13 to 3116
COMMENT     Backbone from pDONR 221 2897 to 651
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    680..1293
                     /label=GFP w/ syntrons
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     536..553
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1294..1327
                     /label=LoxP
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(3882..3902)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    848..898
                     /label=syntron1
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(3862..3883)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1268..1293
                     /label=syntron3
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      4891..5577
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1053..1103
                     /label=syntron2
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             4015..4821
                     /label=KanR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3485..3734
                     /label=GFP w/ syntrons(1)
                     /ApEinfo_label=GFP w/ syntrons
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3485..3509
                     /label=syntron3(1)
                     /ApEinfo_label=syntron3
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     join(570..615,617..651)
                     /label=attP1
                     /ApEinfo_fwdcolor=plum
                     /ApEinfo_revcolor=plum
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3451..3484
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(3756..3844)
                     /label=attP2
                     /ApEinfo_fwdcolor=plum
                     /ApEinfo_revcolor=plum
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(1333..3450)
                     /label=C.Briggsae unc-119(+)
                     /ApEinfo_fwdcolor=#b8b5ff
                     /ApEinfo_revcolor=#b8b5ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(1617..2462)
                     /label=CB-unc-119(+)
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2152..2194)
                     /label=Intron
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1724..1774)
                     /label=Intron(1)
                     /ApEinfo_label=Intron
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2311..2405)
                     /label=Intron(2)
                     /ApEinfo_label=Intron
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    669..675
                     /label=SapI
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    677..679
                     /label=ATG Overlap
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3739..3745
                     /label=SapI(1)
                     /ApEinfo_label=SapI
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3735..3737
                     /label=GGT Overlap
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT Tgtacaaaaa
      661 agcaggctGC TCTTCgATGA GCAAAGGAGA AGAACTTTTC ACTGGAGTTG TCCCAATTCT
      721 TGTTGAATTA GATGGTGATG TTAATGGGCA CAAATTTTCT GTCAGTGGAG AGGGTGAAGG
      781 TGATGCAACA TACGGAAAAC TTACCCTTAA ATTTATTTGC ACTACTGGAA AACTACCTGT
      841 TCCATGGgta agtttaaaca tatatatact aactaaccct gattatttaa attttcagCC
      901 AACACTTGTC ACTACTTTCT GTTATGGTGT TCAATGCTTC TCGAGATACC CAGATCATAT
      961 GAAACGGCAT GACTTTTTCA AGAGTGCCAT GCCCGAAGGT TATGTACAGG AAAGAACTAT
     1021 ATTTTTCAAA GATGACGGGA ACTACAAGAC ACgtaagttt aaacagttcg gtactaacta
     1081 accatacata tttaaatttt cagGTGCTGA AGTCAAGTTT GAAGGTGATA CCCTTGTTAA
     1141 TAGAATCGAG TTAAAAGGTA TTGATTTTAA AGAAGATGGA AACATTCTTG GACACAAATT
     1201 GGAATACAAC TATAACTCAC ACAATGTATA CATCATGGCA GACAAACAAA AGAATGGAAT
     1261 CAAAGTTgta agtttaaaca tgattttact aacATAACTT CGTATAATGT ATGCTATACG
     1321 AAGTTATttc tagacattct ctaatgaaaa aatctttcag ttgaaattga aaatgagtta
     1381 aagttggagt ttttattgaa aacagatttc cgtgtgatta gtgtttttag cgagtgtgac
     1441 aggacagcga aaaaatatag aaacaagggg ggaactgaaa agcttaggaa tgcattgaac
     1501 atgagaaggg gaaggggaag gaacaaacta gacaggaatt attggaattt aatcacattt
     1561 ggagtttttt ttctattcga cagaataatt atccagaaca tttttgtatt aaatatTTAT
     1621 GCATCATATG AGTAGTCGGC TTTGTTGTGC ATGACGAGTT TGTTATCGAC GAAATAGAAG
     1681 CTGTCAGAAC GAGTCTCGTT TGGATTGTTG ATCATGTCGT CCActgaaaa agagattagt
     1741 cttttgaatt gtacttttta gaataatgac tcacTGAGTT GTTGAGAGAG TTGAGGGAAC
     1801 TCATAGATAT GTTCACAGTT GTTTCGTGAA TTCGGAATAC AGAATCCGAA TTCAAAGTCA
     1861 AAACACTTCA GAAGGCGATC TTTGAAGAAG TGACGTTCGA TCATTCGGAA ATGATGGATT
     1921 GGGATGTCTC CTACTTTGAA TTCCACAGTT GCTCCGACCG TTTTGAGTTT CAAAAAGTTT
     1981 GGAGCAAATC TATAACGCAC GTATCTTGCC GACTCCTGTG GCGACTCATC ATTCTCCTGA
     2041 TCGTTCTCCG GTTTGGCGAT CTCGAAAAGC ACTTGCTCAG TGTCCAGATC ACGGATTTGG
     2101 AACTTGGTGA ACTCGATGTT ATAGATGTTC GCAGATGGGG AGCATAAGAA Tcctaaattt
     2161 atgttttaaa ctgaaatcca aagggagcaa gataCCTTGA GTGATTCCCG GAAGTGCTAA
     2221 AACGTCGTTC GGAGTGATTT GAGCTTTCTT CGCAAGCTCC GATTCCGTTG TGATTCCTTG
     2281 TTCGGTGCTT GGTGGTGGCC GTGGCATCTG gaaatatgga aaagttcaac aaaaagaaaa
     2341 gagaaaagaa tgaaatcgga tatcaagagt tagttgagcg gtttctctag ttttctgagt
     2401 ctcacCTGCG ACGGGAAGGT CGCCGAGCCG GGTGGAATCG ATTGTTGTTG CTCGGCTTTC
     2461 ATatcggttt ggttggaagc ggctgaaaac ggaaagaagt ggaagaagga aaagagtgtg
     2521 gtgtgacagg aaaatggtaa ttagagggtg ccaaataacc agctatattt tgtttttttt
     2581 tgaaaacatt tttaaaaaga aaaatacgat aatgatatca gatggatttc cggaaaactg
     2641 gtatgaaaaa tttcaacctt tttgagtaca tgtaatcaaa atacactttg taaattatca
     2701 tttttattga aactccacca tttttctatt tataacgcta ataatttgaa aaagaaacct
     2761 gttgcgaacc gcggggtgaa tcccaaaaac gaatgcgttt tggtggagtg attgattcga
     2821 atcgaagaag aaaaagaaga agacgtggaa tagagagctc actcttaacc gagcagcaca
     2881 caccgacaga aaaaaaaatg aaatgaatga gggtcttctt cttcttcttc ttcgaatgat
     2941 tgacagaaat gggaaaaaga ggaagattga gaagggaaaa aggaaggaga aaagaagcag
     3001 aagaagacgt cagagaggag aggaacgagc ggaaaagcag cgggcgcaag tcatagaagt
     3061 agcagagctg gggagaagaa gacactatcc aagaaaggaa tgacgagaga gtatgcaaag
     3121 gggtataggg tgcagacaga ataggaacag aataacagat gatgagccaa gaagagttga
     3181 aaagggcgat gaatttgtca tgtaacttaa tttgggtcaa tttgagcatg atgaattgaa
     3241 atcatccctt gttgggagtt aataaccggt ttgttatcag aaaccctgta atagaagggc
     3301 gccctaactt tgagccaatt catcccggtt tctgtcaaat atatcaaaaa gtggtcaact
     3361 gacaaattgt ttttgatatt ataataaaca ttttatccgt taacaatttt cgaatacttt
     3421 ttacaaggac ttggataaat tggctcaaag ATAACTTCGT ATAATGTATG CTATACGAAG
     3481 TTATtaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC ATTGAAGATG
     3541 GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC
     3601 TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA
     3661 AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG
     3721 ATGAACTATA CAAAGGTcGA AGAGCaccca gctttCTTGT ACAAAGTTGG CATTATAAGA
     3781 AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT
     3841 ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC
     3901 TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAATAATA
     3961 TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC
     4021 CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT
     4081 GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT
     4141 GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT
     4201 GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC
     4261 AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA
     4321 ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG
     4381 GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT
     4441 CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT
     4501 GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA
     4561 CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT
     4621 ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC
     4681 CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG
     4741 AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT
     4801 TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC
     4861 ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTAC
     4921 GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT
     4981 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT
     5041 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG
     5101 CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT
     5161 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC
     5221 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG
     5281 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA
     5341 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG
     5401 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG
     5461 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA
     5521 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT
     5581 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T 
//