LOCUS       pMLS281                 3124 bp ds-DNA     circular     03-FEB-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Backbone from pDONR 221 2897 to 651
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    652..670
                     /label=attB1-F pimer site
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1176..1200
                     /label=syntron3
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     536..553
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1142..1155
                     /label=FRT site
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    671..677
                     /label=SapI Recognition Site
                     /ApEinfo_fwdcolor=#ff5375
                     /ApEinfo_revcolor=#ff5375
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1020..1024
                     /label=polyA site
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             773..892
                     /label=Mm ODC PEST
                     /ApEinfo_fwdcolor=#ff29a8
                     /ApEinfo_revcolor=#ff29a8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1156..1175
                     /label=FRT site(1)
                     /ApEinfo_label=FRT site
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(1385..1405)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     prim_transcript 896..1140
                     /label=Operon intergenic region
                     /ApEinfo_fwdcolor=#d1975f
                     /ApEinfo_revcolor=#d1975f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(1259..1347)
                     /label=attP2
                     /ApEinfo_fwdcolor=plum
                     /ApEinfo_revcolor=plum
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1115..1120
                     /label=gpd-2/3 SL2
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(679..681)
                     /label=Sap Trap gg Overlap
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1006..1011
                     /label=polyA signal
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(1365..1386)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    893..895
                     /label=Stop
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1248..1258
                     /label=attB2 primer site
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1204..1206
                     /label=New Feature
                     /ApEinfo_fwdcolor=#1bd525
                     /ApEinfo_revcolor=#1bd525
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      2394..3080
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    745..769
                     /label=syntron3(1)
                     /ApEinfo_label=syntron3
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1241..1247)
                     /label=SapI Recognition Site(1)
                     /ApEinfo_label=SapI Recognition Site
                     /ApEinfo_fwdcolor=#ff5375
                     /ApEinfo_revcolor=#ff5375
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    685..710
                     /label=syntron3(2)
                     /ApEinfo_label=syntron3
                     /ApEinfo_fwdcolor=#a9e4ff
                     /ApEinfo_revcolor=#a9e4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1518..2324
                     /label=KanR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    682..684
                     /label=Ser
                     /ApEinfo_fwdcolor=#a0a295
                     /ApEinfo_revcolor=#a0a295
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1237..1239
                     /label=Sap Trap gg Overlap(1)
                     /ApEinfo_label=Sap Trap gg Overlap
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    711..724
                     /label=FRT site(2)
                     /ApEinfo_label=FRT site
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1207..1236
                     /label=N-tag Linker
                     /ApEinfo_fwdcolor=#75756d
                     /ApEinfo_revcolor=#75756d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    770..772
                     /label=Gly
                     /ApEinfo_fwdcolor=#a0a295
                     /ApEinfo_revcolor=#a0a295
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     join(570..615,617..651)
                     /label=attP1
                     /ApEinfo_fwdcolor=plum
                     /ApEinfo_revcolor=plum
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    725..744
                     /label=FRT site(3)
                     /ApEinfo_label=FRT site
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT Tgtacaaaaa
      661 agcaggctat GCTCTTCgGG TTCGgtaagt ttaaacatga ttttactaac GAAGTTCCTA
      721 TTCTCTAGAA AGTATAGGAA CTTCtaacta atctgattta aattttcagG GTAGCCATGG
      781 CTTCCCGCCG GAGGTGGAGG AGCAGGATGA TGGCACGCTG CCCATGTCTT GTGCCCAGGA
      841 GAGCGGGATG GACCGTCACC CTGCAGCCTG TGCTTCTGCT AGGATCAATG TGTAAgctgt
      901 ctcatcctac tttcacctag ttaactgctt gtcttaaaat ctatgcttct ctttagtatc
      961 taaaattttc ctagaagctt acaagtatat aaatggtctc ttctcaataa aggttgtata
     1021 tttattcatc ttattgaatc tgccatttcc tcgtttttgc gagtttatat accttccaat
     1081 tttctttcta ttgtattttc aacttctaat tttaattcag ggaaactgct tcaacgcatc
     1141 aGAAGTTCCT ATTCTCTAGA AAGTATAGGA ACTTCtaact aatctgattt aaattttcag
     1201 GGTATGGGCA GCGGAGGTAC CGGCGGTAGT GGAGGCACGc GAAGAGCtac ccagctttCT
     1261 TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA
     1321 CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT
     1381 ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT
     1441 ACATTGCACA AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA
     1501 GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT
     1561 CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG
     1621 GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG
     1681 GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG
     1741 AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC
     1801 TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG
     1861 GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT
     1921 GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA
     1981 ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC
     2041 AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG
     2101 GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG
     2161 TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG
     2221 GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG
     2281 ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA
     2341 ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC
     2401 CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
     2461 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
     2521 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
     2581 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT
     2641 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
     2701 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
     2761 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
     2821 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
     2881 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
     2941 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
     3001 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
     3061 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
     3121 TGTT     
//