LOCUS       Exported               12566 bp ds-DNA     circular SYN 29-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    prs416-dcas9-mxi1-prpr1teto-grna-tetr-short-name-prs416gt-mxi1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 29, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12566
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="CEN/ARS"
     promoter        610..714
                     /label=AmpR promoter
                     /note="AmpR promoter"
     CDS             715..783
                     /label=AmpR
                     /note="AmpR"
     CDS             784..1575
                     /label=AmpR
                     /note="AmpR"
     rep_origin      1746..2334
                     /label=ori
                     /note="ori"
     misc_feature    2365..2400
                     /label=Pci1-UP
                     /note="Pci1-UP"
     promoter        2401..2812
                     /label=pRPR1
                     /note="pRPR1 promoter"
     misc_feature    2728..2812
                     /label=RNAse P leader sequence
                     /note="RNAse P leader sequence"
     misc_feature    2728
                     /label=TSS
                     /note="TSS"
     misc_feature    2729..2747
                     /label=TetO
                     /note="TetO"
     misc_feature    2820..2901
                     /label=sgRNA
                     /note="sgRNA"
     terminator      2908..3345
                     /label=RPR1t
                     /note="RPR1t terminator"
     promoter        3354..4097
                     /label=pGPM1
                     /note="pGPM1 promoter"
     misc_feature    3973..3998
                     /label=497_GPM1_FwdSeqPrime
                     /note="497_GPM1_FwdSeqPrime"
     CDS             4105..4728
                     /label=TetR
                     /note="TetR"
     terminator      4729..5126
                     /label=tGPM1
                     /note="tGPM1 terminator"
     misc_feature    complement(4804..4832)
                     /label=498_GPM1t_RevSeqPrime
                     /note="498_GPM1t_RevSeqPrime"
     primer_bind     complement(5097..5126)
                     /label=211_tGPM1_RevPrime
                     /note="211_tGPM1_RevPrime"
     promoter        5395..5400
                     /label=lac promoter
                     /note="lac promoter"
     promoter        5401..5418
                     /label=lac promoter
                     /note="lac promoter"
     promoter        5419..5425
                     /label=lac promoter
                     /note="lac promoter"
     protein_bind    5433..5449
                     /label=lac operator
                     /note="lac operator"
     misc_feature    5452..5460
                     /label=M13_pUC_rev_primer
                     /note="M13_pUC_rev_primer"
     primer_bind     5457..5477
                     /label=M13-rev
                     /note="M13-rev"
     misc_feature    5459..5477
                     /label=M13_reverse_primer
                     /note="M13_reverse_primer"
     promoter        5494..5513
                     /label=T3_promoter
                     /note="T3_promoter"
     primer_bind     5495..5514
                     /label=T3
                     /note="T3"
     misc_feature    5530..5936
                     /label=TEF Promoter
                     /note="TEF Promoter"
     misc_feature    complement(5530..5568)
                     /label=M13_pUC_rev_primer
                     /note="M13_pUC_rev_primer"
     CDS             5939..5980
                     /label=dCas9_VP64m
                     /note="dCas9_VP64m"
     misc_feature    5948..5974
                     /label=NLS
                     /note="NLS"
     misc_feature    5981..10096
                     /label=dCas9(Church)
                     /note="dCas9(Church)"
     misc_feature    10103..10126
                     /label=NLS
                     /note="NLS"
     misc_feature    10161..10282
                     /label=MxiI Domain
                     /note="MxiI Domain"
     misc_feature    10304
                     /label=XhoI
                     /note="XhoI"
     misc_feature    10305..10306
                     /label=CYC1 Terminator
                     /note="CYC1 Terminator"
     misc_feature    10307..10562
                     /label=CYC1 Terminator
                     /note="CYC1 Terminator"
     primer_bind     complement(10571..10591)
                     /label=T7
                     /note="T7"
     promoter        complement(10572..10590)
                     /label=T7_promoter
                     /note="T7_promoter"
     primer_bind     complement(10600..10617)
                     /label=M13-fwd
                     /note="M13-fwd"
     misc_feature    complement(10600..10616)
                     /label=M13_forward20_primer
                     /note="M13_forward20_primer"
     misc_feature    10602..10617
                     /label=lacZ_a
                     /note="lacZ_a"
     misc_feature    complement(10609..10617)
                     /label=M13_pUC_fwd_primer
                     /note="M13_pUC_fwd_primer"
     CDS             10618..10978
                     /label=lacZ-alpha
                     /note="lacZ-alpha"
     rep_origin      10761..11216
                     /label=f1 ori
                     /note="f1 ori"
     CDS             complement(11347..12150)
                     /label=URA3
                     /note="URA3"
     promoter        complement(12151..12371)
                     /label=URA3 promoter
                     /note="URA3 promoter"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctgg ccttttgctc
     2401 gggggatctg ccaattgaac ataacatggt agttacatat actagtaata tggttcggca
     2461 cacattaaaa gtataaaaac tatctgaatt acgaattaca tatattggtc ataaaaatca
     2521 atcaatcatc gtgtgtttta tatgtctctt atctaagtat aagaatatcc atagttaata
     2581 ttcacttacg ctacctttta acctgtaatc attgtcaaca ggatatgtta acgacccaca
     2641 ttgataaacg ctagtatttc tttttcctct tcttattggc cggctgtctc tatactcccc
     2701 tatagtctgt ttcttttcgt ttcgattgtc cctatcagtg atagagatgg cgcacatggt
     2761 acgctgtggt gctcgcggct gggaacgaaa ctctgggagc tgcgattggc agcggccgcg
     2821 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     2881 gcaccgagtc ggtgcttttt tctcgagcca tatccaactt ccaatttaat ctttcttttt
     2941 taattttcac ttatttgcga tacagaaaga aaaaagcgat agtaactatt gaattttgtt
     3001 tggatttggt tagattagat atggtttctc tttatattta catgctaaaa atgggctaca
     3061 ccagagatac ataattagat atatatacgc cagtacacct tatcggccca agccttgtcc
     3121 caaggcagcg ttttgttctt ggaaacgctg ccctacacgt tcgctatgct tcaagaactt
     3181 ttctgagcac ttcatgatgc atgtttgttc cttattggtt agctttgatg ttgtgaagtc
     3241 attgacacag tctgtgaaac atctttctac cagattagag tacaaacgca tgaaatcctt
     3301 catttgcttt tgttccacta ctttttggaa ctcttgttgt tctttcctgc aggtagtcgt
     3361 gcaatgtatg actttaagat ttgtgagcag gaagaaaagg gagaatcttc taacgataaa
     3421 cccttgaaaa actgggtaga ctacgctatg ttgagttgct acgcaggctg cacaattaca
     3481 cgagaatgct cccgcctagg atttaaggct aagggacgtg caatgcagac gacagatcta
     3541 aatgaccgtg tcggtgaagt gttcgccaaa cttttcggtt aacacatgca gtgatgcacg
     3601 cgcgatggtg ctaagttaca tatatatata tatgtatagc catagtgatg tctaagtaac
     3661 ctttatggta tatttcttaa tgtggaaaga tactagcgcg cgcacccaca cacaagcttc
     3721 gtcttttctt gaagaaaaga ggaagctcgc taaatgggat tccactttcc gttccctgcc
     3781 agctgatgga aaaaggttag tggaacgatg aagaataaaa agagagatcc actgaggtga
     3841 aatttcagct gacagcgagt ttcatgatcg tgatgaacaa tggtaacgag ttgtggctgt
     3901 tgccagggag ggtggttctc aacttttaat gtatggccaa atcgctactt gggtttgtta
     3961 tataacaaag aagaaataat gaactgattc tcttcctcct tcttgtcctt tcttaattct
     4021 gttgtaatta ccttcctttg taattttttt tgtaattatt cttcttaata atccaaacaa
     4081 acacacatat tacaatatat taaaatgtct agattagata aaagtaaagt gattaacagc
     4141 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     4201 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
     4261 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     4321 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     4381 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     4441 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     4501 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     4561 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     4621 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     4681 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaagt ctgaagaatg
     4741 aatgatttga tgatttcttt ttccctccat ttttcttact gaatatatca atgatataga
     4801 cttgtatagt ttattatttc aaattaagta gctatatata gtcaagataa cgtttgtttg
     4861 acacgattac attattcgtc gacatctttt ttcagcctgt cgtggtagca atttgaggag
     4921 tattattaat tgaataggtt cattttgcgc tcgcataaac agttttcgtc agggacagta
     4981 tgttggaatg agtggtaatt aatggtgaca tgacatgtta tagcaataac cttgatgttt
     5041 acatcgtagt ttaatgtaca ccccgcgaat tcgttcaagt aggagtgcac caattgcaaa
     5101 gggaaaagct gaatgggcag ttcgaaccgc ggtctttcct gcgttatccc ctgattctgt
     5161 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5221 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5281 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5341 cagtgagcgc aacgcaatta atgtgagtta cctcactcat taggcacccc aggctttaca
     5401 ctttatgctt ccggctccta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     5461 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     5521 gctggagctc atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg
     5581 actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa atttcccctc
     5641 tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaga
     5701 ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt
     5761 tcttgaaaat tttttttttg atttttttct ctttcgatga cctcccattg atatttaagt
     5821 taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt
     5881 tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt ttctagagat
     5941 gtctagagcc ccaaagaaga agagaaaagt tagacccggg atggacaaga agtactccat
     6001 tgggctcgct atcggcacaa acagcgtcgg ctgggccgtc attacggacg agtacaaggt
     6061 gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc cacagcataa agaagaacct
     6121 cattggcgcc ctcctgttcg actccgggga gacggccgaa gccacgcggc tcaaaagaac
     6181 agcacggcgc agatataccc gcagaaagaa tcggatctgc tacctgcagg agatctttag
     6241 taatgagatg gctaaggtgg atgactcttt cttccatagg ctggaggagt cctttttggt
     6301 ggaggaggat aaaaagcacg agcgccaccc aatctttggc aatatcgtgg acgaggtggc
     6361 gtaccatgaa aagtacccaa ccatatatca tctgaggaag aagcttgtag acagtactga
     6421 taaggctgac ttgcggttga tctatctcgc gctggcgcat atgatcaaat ttcggggaca
     6481 cttcctcatc gagggggacc tgaacccaga caacagcgat gtcgacaaac tctttatcca
     6541 actggttcag acttacaatc agcttttcga agagaacccg atcaacgcat ccggagttga
     6601 cgccaaagca atcctgagcg ctaggctgtc caaatcccgg cggctcgaaa acctcatcgc
     6661 acagctccct ggggagaaga agaacggcct gtttggtaat cttatcgccc tgtcactcgg
     6721 gctgaccccc aactttaaat ctaacttcga cctggccgaa gatgccaagc ttcaactgag
     6781 caaagacacc tacgatgatg atctcgacaa tctgctggcc cagatcggcg accagtacgc
     6841 agaccttttt ttggcggcaa agaacctgtc agacgccatt ctgctgagtg atattctgcg
     6901 agtgaacacg gagatcacca aagctccgct gagcgctagt atgatcaagc gctatgatga
     6961 gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga cagcaactgc ctgagaagta
     7021 caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc ggatacattg acggcggagc
     7081 aagccaggag gaattttaca aatttattaa gcccatcttg gaaaaaatgg acggcaccga
     7141 ggagctgctg gtaaagctta acagagaaga tctgttgcgc aaacagcgca ctttcgacaa
     7201 tggaagcatc ccccaccaga ttcacctggg cgaactgcac gctatcctca ggcggcaaga
     7261 ggatttctac ccctttttga aagataacag ggaaaagatt gagaaaatcc tcacatttcg
     7321 gataccctac tatgtaggcc ccctcgcccg gggaaattcc agattcgcgt ggatgactcg
     7381 caaatcagaa gagaccatca ctccctggaa cttcgaggaa gtcgtggata agggggcctc
     7441 tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa aatctgccta acgaaaaggt
     7501 gcttcctaaa cactctctgc tgtacgagta cttcacagtt tataacgagc tcaccaaggt
     7561 caaatacgtc acagaaggga tgagaaagcc agcattcctg tctggagagc agaagaaagc
     7621 tatcgtggac ctcctcttca agacgaaccg gaaagttacc gtgaaacagc tcaaagaaga
     7681 ctatttcaaa aagattgaat gtttcgactc tgttgaaatc agcggagtgg aggatcgctt
     7741 caacgcatcc ctgggaacgt atcacgatct cctgaaaatc attaaagaca aggacttcct
     7801 ggacaatgag gagaacgagg acattcttga ggacattgtc ctcaccctta cgttgtttga
     7861 agatagggag atgattgaag aacgcttgaa aacttacgct catctcttcg acgacaaagt
     7921 catgaaacag ctcaagaggc gccgatatac aggatggggg cggctgtcaa gaaaactgat
     7981 caatgggatc cgagacaagc agagtggaaa gacaatcctg gattttctta agtccgatgg
     8041 atttgccaac cggaacttca tgcagttgat ccatgatgac tctctcacct ttaaggagga
     8101 catccagaaa gcacaagttt ctggccaggg ggacagtctt cacgagcaca tcgctaatct
     8161 tgcaggtagc ccagctatca aaaagggaat actgcagacc gttaaggtcg tggatgaact
     8221 cgtcaaagta atgggaaggc ataagcccga gaatatcgtt atcgagatgg cccgagagaa
     8281 ccaaactacc cagaagggac agaagaacag tagggaaagg atgaagagga ttgaagaggg
     8341 tataaaagaa ctggggtccc aaatccttaa ggaacaccca gttgaaaaca cccagcttca
     8401 gaatgagaag ctctacctgt actacctgca gaacggcagg gacatgtacg tggatcagga
     8461 actggacatc aatcggctct ccgactacga cgtggatgct atcgtgcccc agtcttttct
     8521 caaagatgat tctattgata ataaagtgtt gacaagatcc gataaaaata gagggaagag
     8581 tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa aattattggc ggcagctgct
     8641 gaacgccaaa ctgatcacac aacggaagtt cgataatctg actaaggctg aacgaggtgg
     8701 cctgtctgag ttggataaag ccggcttcat caaaaggcag cttgttgaga cacgccagat
     8761 caccaagcac gtggcccaaa ttctcgattc acgcatgaac accaagtacg atgaaaatga
     8821 caaactgatt cgagaggtga aagttattac tctgaagtct aagctggtct cagatttcag
     8881 aaaggacttt cagttttata aggtgagaga gatcaacaat taccaccatg cgcatgatgc
     8941 ctacctgaat gcagtggtag gcactgcact tatcaaaaaa tatcccaagc ttgaatctga
     9001 atttgtttac ggagactata aagtgtacga tgttaggaaa atgatcgcaa agtctgagca
     9061 ggaaataggc aaggccaccg ctaagtactt cttttacagc aatattatga attttttcaa
     9121 gaccgagatt acactggcca atggagagat tcggaagcga ccacttatcg aaacaaacgg
     9181 agaaacagga gaaatcgtgt gggacaaggg tagggatttc gcgacagtcc ggaaggtcct
     9241 gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta cagaccggag gcttctccaa
     9301 ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc gcacgcaaaa aagattggga
     9361 ccccaagaaa tacggcggat tcgattctcc tacagtcgct tacagtgtac tggttgtggc
     9421 caaagtggag aaagggaagt ctaaaaaact caaaagcgtc aaggaactgc tgggcatcac
     9481 aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac tttctcgagg cgaaaggata
     9541 taaagaggtc aaaaaagacc tcatcattaa gcttcccaag tactctctct ttgagcttga
     9601 aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg cagaaaggta acgagctggc
     9661 actgccctct aaatacgtta atttcttgta tctggccagc cactatgaaa agctcaaagg
     9721 gtctcccgaa gataatgagc agaagcagct gttcgtggaa caacacaaac actaccttga
     9781 tgagatcatc gagcaaataa gcgaattctc caaaagagtg atcctcgccg acgctaacct
     9841 cgataaggtg ctttctgctt acaataagca cagggataag cccatcaggg agcaggcaga
     9901 aaacattatc cacttgttta ctctgaccaa cttgggcgcg cctgcagcct tcaagtactt
     9961 cgacaccacc atagacagaa agcggtacac ctctacaaag gaggtcctgg acgccacact
    10021 gattcatcag tcaattacgg ggctctatga aacaagaatc gacctctctc agctcggtgg
    10081 agacagcagg gctgacgagg gagctcccaa gaaaaagcgc aaggtaggta gttccaagct
    10141 tggcggcagc ggcggcagca tggaacgtgt gagaatgatt aatgtgcaaa ggctgttaga
    10201 agccgcagag tttttagaaa gaagagaaag agaatgcgaa cacgggtatg ccagttcttt
    10261 ccctagcatg ccctctccca gaggctaact cgagctcttc tcgagtcatg taattagtta
    10321 tgtcacgctt acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta
    10381 gacaacctga agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt
    10441 tatttatatt tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta
    10501 tactgaaaac cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcggccggt
    10561 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
    10621 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
    10681 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
    10741 cctgaatggc gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    10801 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    10861 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    10921 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    10981 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    11041 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    11101 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    11161 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcctg
    11221 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg gtaataactg
    11281 atataattaa attgaagctc taatttgtga gtttagtata catgcattta cttataatac
    11341 agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta
    11401 acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat
    11461 aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc
    11521 tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct
    11581 tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat
    11641 gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg acaattctgc
    11701 taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct tcaaaccgct
    11761 aacaatacct gggcccacca caccgtgtgc attcgtaatg tctgcccatt ctgctattct
    11821 gtatacaccc gcagagtact gcaatttgac tgtattacca atgtcagcaa attttctgtc
    11881 ttcgaagagt aaaaaattgt acttggcgga taatgccttt agcggcttaa ctgtgccctc
    11941 catggaaaaa tcagtcaaga tatccacatg tgtttttagt aaacaaattt tgggacctaa
    12001 tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag cacacaagtt
    12061 tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag gatgagtagc
    12121 agcacgttcc ttatatgtag ctttcgacat gatttatctt cgtttcctgc aggtttttgt
    12181 tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg
    12241 tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac
    12301 cgaatcaaaa aaatttcaaa gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga
    12361 attgaattga aaagctgtgg tatggtgcac tctcagtaca atctgctctg atgccgcata
    12421 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
    12481 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
    12541 ttcaccgtca tcaccgaaac gcgcga
//
