LOCUS       pHIS8_4_FF_STX_c        6414 bp ds-DNA     circular     20-AUG-2015
DEFINITION  Complementary copy of pET-28a(+).
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|532134159|
COMMENT     VNTDBDATE|532134296|
COMMENT     VNTNAME|pHIS8-3|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             2920..2944
                     /dnas_title="lac operator"
                     /vntifkey="4"
                     /label=lac operator
                     /ApEinfo_fwdcolor=#ffa249
                     /ApEinfo_revcolor=#4f86ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      4343..4798
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(4894..5706)
                     /dnas_title="kan sequence"
                     /vntifkey="4"
                     /label=kan sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      1..1
                     /dnas_title="ColE1 pBR322 origin"
                     /vntifkey="33"
                     /label=ColE1 pBR322 origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(1435..2514)
                     /dnas_title="lac I"
                     /vntifkey="4"
                     /label=lac I
                     /ApEinfo_fwdcolor=#ff8d89
                     /ApEinfo_revcolor=#ff728a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      4260..4306
                     /dnas_title="T7 terminator"
                     /vntifkey="43"
                     /label=T7 terminator
                     /ApEinfo_fwdcolor=#ff1ece
                     /ApEinfo_revcolor=#34ff2b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    4175..4192
                     /dnas_title="His tag"
                     /vntifkey="31"
                     /label=His tag
                     /ApEinfo_fwdcolor=#1db0ff
                     /ApEinfo_revcolor=#ff2d37
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2901..2917
                     /dnas_title="T7 promoter"
                     /vntifkey="30"
                     /label=T7 promoter
                     /ApEinfo_fwdcolor=#f9ff4a
                     /ApEinfo_revcolor=#5769ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     RBS             2975..2980
                     /dnas_title="rbs"
                     /vntifkey="32"
                     /label=ribosomal binding site (rbs)
                     /ApEinfo_fwdcolor=#5dff56
                     /ApEinfo_revcolor=#ff5ff3
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2994..3017
                     /dnas_title="N-His tag"
                     /vntifkey="21"
                     /label=N-His tag
                     /ApEinfo_fwdcolor=#43c1ff
                     /ApEinfo_revcolor=#ff6561
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2988..2990
                     /note="Start Codon"
                     /label=Start Codon
                     /ApEinfo_fwdcolor=#39ff33
                     /ApEinfo_revcolor=#ff48ea
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3024..3044
                     /note="TE Protease Site"
                     /label=TE Protease Site
                     /ApEinfo_fwdcolor=#7fffb5
                     /ApEinfo_revcolor=#ff65f5
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4122..4122
                     /note="TE Protease Site"
                     /label=TE Protease Site(1)
                     /ApEinfo_label=TE Protease Site
                     /ApEinfo_fwdcolor=#7fffb5
                     /ApEinfo_revcolor=#ff65f5
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3044..3049
                     /note="NcoI Diagnostic Digest Site"
                     /label=NcoI Diagnostic Digest Site
                     /ApEinfo_fwdcolor=#ff000a
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4122..4127
                     /note="NcoI Diagnostic Digest Site"
                     /label=NcoI Diagnostic Digest Site(1)
                     /ApEinfo_label=NcoI Diagnostic Digest Site
                     /ApEinfo_fwdcolor=#ff000a
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3051..4121
                     /label=Firefly sulfotransferase Ctl 2 TR12749_c0_g2_i1_m.9
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
       61 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      181 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
      241 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
      301 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
      361 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
      421 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
      481 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
      541 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
      601 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
      661 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
      721 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
      781 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
      841 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
      901 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
      961 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     1021 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     1081 cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
     1141 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     1201 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     1261 gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata
     1321 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     1381 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
     1441 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1501 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
     1561 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     1621 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     1681 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
     1741 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     1801 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     1861 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
     1921 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
     1981 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
     2041 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
     2101 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
     2161 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
     2221 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     2281 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     2341 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     2401 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     2461 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     2521 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
     2581 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
     2641 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
     2701 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     2761 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     2821 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
     2881 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
     2941 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg aaacaccacc
     3001 accaccacca ccaccacggt ggtGAAAACT TGTACTTCCA GGCCcatggc ATGCCACATA
     3061 ACATTCAAAT TGGGGATATT TTAAATAATA GAGATGAAAA TAGTAAGAGG AAATTTCCTT
     3121 ACGAAGTTAA AATTGTAGAT CCTGACACAA ATAAGGAGCT ATTGAATGTT TTTACAGGGG
     3181 AGCATACAGG GTTCGTACAA GTAGGCCCTC AAAAATATTT CTTTCCTAGC GGCTATGAAA
     3241 AGGTAGCAGA AAAATATTAC AATTTTGAAA TACGACCCGA CGATGTCTGG ATTGTTACAT
     3301 TTCCAAGATC AGGTACAACG TGGACGCAAG AAATGGTGTG GTTAATTGCA AACGAATTTG
     3361 ACTACAATAC GGCCGCTAGA GTACCATTAA ACGAAAGATT TCCTTTTTTA GAATTCAGCA
     3421 TGTGCGTCCA TCCGAAAATG AAGGCACAAT TCTTACAAGA AAATAGTCAC AGTATTGAAC
     3481 GACAAAATAT GATCGAAGAC GTCTGCACAG AAGCATGGAA GATTTTGGAA AATTGGAAGG
     3541 GGAGGCGTTT CATCAAAACA CACCTGCCAT TCAGCCTGTT GCCACCTAAT TTGTTAACCT
     3601 CTGGCTGTAA GGTAATTTAC GTTGCAAGAA ACCCGAAGGA TGTTATGGTC TCGTACTACT
     3661 ATTTGAACAG ATTATTTCGA ACCCAAGGGT ATCGGGGTGA CTTTCCGACA TTCGCGGAGT
     3721 ACTTCCTAAA GGATATGGTA CATTGGACGC CGTTTTGGTC TCATTTACAA GAAGGCTGGG
     3781 ATTTGCGAAG CTCCAAGAAT CTTCTTTTTC TGTTCTACGA CCAAATGAAT AAATTGCAGA
     3841 CAATCCAAAA GGTGTCGAAC TTTTTGGGGA AGGAAATTGA TGACGATCAA TTAACGAAAA
     3901 TACAGAATCA TTTACAAATT GAGAACTTTA AGAATAATAA GTCGCTCAAC GCTAATGATT
     3961 TATTAGAATT GGGAATATTA TATGGAGGCG AAGAGGGTTT TGTAAGGAAG GGAAAGACGG
     4021 GATCACATAC GGACTTATCC AATGTCGAAT TAGACGAGAA GATTGACAAG TGGGTCAAAA
     4081 AGAATTTACA GATGACGAAT ATACCGTTTG AACGAATGTA ACcatggcgg atccgaattc
     4141 gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
     4201 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     4261 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     4321 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4381 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4441 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4501 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4561 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4621 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4681 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4741 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttca
     4801 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4861 tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa
     4921 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     4981 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     5041 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     5101 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     5161 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     5221 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     5281 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     5341 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     5401 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     5461 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     5521 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     5581 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     5641 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     5701 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgacca
     5761 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5821 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5881 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5941 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     6001 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6061 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6121 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6181 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6241 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6301 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6361 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tgga
//
