LOCUS       pBS-TarHom-Tol2-        7259 bp    DNA     linear       01-MAR-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(5123..5146)
                     /note="M13Rev"
     misc_feature    complement(5069..5088)
                     /note="T3"
     misc_feature    94..109
                     /note="M13F(-20)"
     misc_feature    120..139
                     /note="T7"
     misc_feature    399..1218
                     /note="Tol2 Exon4"
     misc_feature    153..382
                     /note="5 prime arm of homology for pTARBAC"
     misc_feature    153..173
                     /note="forward primer for amplification of targeting
                     cassette"
     misc_feature    2546..3099
                     /note="Tol2 Exon1"
     misc_feature    3106..3825
                     /note="cryaa promoter"
     misc_feature    3826..4503
                     /note="dsRed"
     misc_feature    4791..5038
                     /note="3 prime arm of homology for pTARBAC"
     misc_feature    4565..4749
                     /note="SV40 polyA"
     misc_feature    complement(5018..5038)
                     /note="rev primer for amplification of targeting cassette"
     misc_feature    1225..2539
                     /note="kana resistance cassette"
     source          1..7259
                     /dnas_title="pBS-TarHom-Tol2-FRT-GalK-cryaa-dsRed-correcte
                     d"
ORIGIN      
        1 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT
       61 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT
      121 AATACGACTC ACTATAGGGC GAATTGGAGC TCggcggccg ccaggcctac ccactagtca
      181 attcgggagg atcgaaacgg cagatcgcaa aaaacagtac atacagaagg agacatgaac
      241 atgaacatca aaaaaattgt aaaacaagcc acagttctga cttttacgac tgcacttctg
      301 gcaggaggag cgactcaagc cttcgcgaaa gaaaataacc aaaaagcata caaagaaacg
      361 tacgcgtctc tcatattaca cgCCGCGGTG GCGGCCGCAT CTGGCCTTGT TTCAGACACC
      421 AGGGAGTCTC TGCTCACGTT TCCTGCTATT TGCAGCCTCT CTATCAAGAC TAATACACCT
      481 CTTCCCGCAT CGGCTGCCTG TGAGAGGCTT TTCAGCACTG CgGGATTGCT TTTCAGCCCC
      541 AAAAGAGCTA GGCTTGACAC TAACAATTTT GAGAATCAGC TTCTACTGAA GTTAAATCTG
      601 AGGTTTTACA ACTTTGAGTA GCGTGTACTG GCgTTAGATT GTCTGTCTTA TAGTTTGATA
      661 ATTAAATACA AACAGTTCTA AAGCAGGATA AAACCTTGTA TGCATTTCAT TTAATGTTTT
      721 TTGAGATTAA AAGCTTAAAC AAGAATCTCT AGTTTTCTTT CTTGCTTTTA CTTTTACTTC
      781 CTTAATACTC AAGTACAATT TTAATGGAGT ACTTTTTTAC TTTTACTCAA GTAAGATTCT
      841 AGCCAGATAC TTTTACTTTT AATTGAGTAA AATTTTCCCT AAGTACTTGT ACTTTCACTT
      901 GAGTAAAATT TTTGAGTACT TTTTACACCT CTGTCAAGAA CTCCTGGACA AACCTCTGAC
      961 CTGTGTGGAA CAGAGTGGAT ATGGGTGTCT GAACAGATAT TCACGTCTTT TGCAGATCAG
     1021 AGGGCATTTC TGGTGTTGTG CAATGACCTG GGTCCAACAG GTAGGCGTAC TCGTATCCAA
     1081 GAGCCTTGTT GGACAGGAGG TAGTCTCCAT TCCTATAAAG AGGAAGGAAT GGCACCATGT
     1141 AGTAGCCGTC ATTGTGGCCA ATTGGTGCAT TGGTACGTGG GTAGATGGAC CGGGGAGGCT
     1201 GATGAGTTCT TAGCCAGCGG ATCTgattac cctgttatcc ctagatttaa atgatatcgg
     1261 atcctagtaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1321 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     1381 agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct
     1441 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     1501 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     1561 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     1621 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     1681 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     1741 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     1801 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     1861 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     1921 atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     1981 gataacctta tttttgacga ggggGaatta aCaggttgta ttgatgttgg acgagtcgga
     2041 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2101 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2161 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     2221 tggcagagca ttacgctgac ttgacgggac ggcggctttg ttgaataaat cgaactcttg
     2281 ctgagttgaa ggatcGgatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc
     2341 aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc
     2401 tcactttctg gctggATGAT Ggggcgattc aggcctggta tgagtcagca acaccttctt
     2461 cacgaggcag acctcagcgg cggccggatc cgatatcgtt tggcgcgcca aactcgctac
     2521 cttaggaccg ttatagttaA GATCCGATCT GCTGGGCTTG CTGAAGGTAG GGGGTCAAGA
     2581 ACCAGAGGTG TAAAGTACTT GAGTAATTTT ACTTGATTAC TGTACTTAAG TATTATTTTT
     2641 GGGGATTTTT ACTTTACTTG AGTACAATTA AAAATCAATA CTTTTACTTT TACTTAATTA
     2701 CATTTTTTTA GAAAAAAAAG TACTTTTTAC TCCTTACAAT TTTATTTACA GTCAAAAAGT
     2761 ACTTATTTTT TGGAGATCAC TTCATTCTAT TTTCCCTTGC TATTACCAAA CCAATTGAAT
     2821 TGCGCTGATG CCCAGTTTAA TTTAAATGTT ATTTATTCTG CCTATGAAAA TCGTTTTCAC
     2881 ATTATATGAA ATTGGTCAGA CATGTTCATT GGTCCTTTGG AAGTGACGTC ATGTCACATC
     2941 TATTACCACA ATGCACAGCA CCTTGACCTG GAAATTAGGG AAATTATAAC AGTCAATCAG
     3001 TGGAAGAAAA TGGAGGAAGT ATGTGATTCA TCAGCAGCTG CGAGCAGCAC AGTCCAAAAT
     3061 CAGCCACAGG ATCAAGAGCA CCCGTGGCCG TATCTTCGCC CCGGGagctt cgaattctaa
     3121 tatggcaata aacgatcttc agagaaactt gcattggtaa atagtttcag cctatgcata
     3181 tgtagttatt caaactgtaa tgactatctt acagtattat atgtttataa tagtttgatc
     3241 caataaatcc agatttatat gcttttcaac ataaaattgt gaaatgcaca gaatgtacat
     3301 atacaataca tttcctacaa taatgctctc ttttctatta atagtgtgca ttcagtgcag
     3361 gatgttatgt atttattttc tgtagtgcaa actttttcaa agaattttcc agtctaagct
     3421 tttcagtcaa gaaaaaaaaa agcgattgat tatctgtgat atttaaaaaa attagctctt
     3481 aaatctggtg aaccaagtgg ccacaacaca ttaaatactg acgttctaca gccatgtagc
     3541 ttcatctggt ctggtttgtt ttggcaggca cttattttcc ggtgatagtt ggccacaaga
     3601 gtattgtctg agccattcag tgctagactg tcattctcag gtcagagtcc accccgctga
     3661 ttctgctgac aacactgttc ccaccatgag ataatgccat tccagagaga tccatttgta
     3721 agcccctctt tctgcagcac aggtatataa ccaggggtct gcctccacta aggccggcac
     3781 acatcatttg gggatctttg tactgtggat ccaccggtcg ccaccatggc cCcctccgag
     3841 gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac
     3901 gaAttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     3961 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     4021 cagtacggct ccaaggtgta cgtgaagcac cccgccgaAa tccccgacta caagaagctg
     4081 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     4141 accgtgaccc aggactcctc cctgcaggac ggctccttca tctacaaggt gaagttcatc
     4201 ggcgtgaact tcccctccga cggccccgta atgcagaaga agactatggg ctgggaggcc
     4261 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca caaggccctg
     4321 aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag
     4381 cccgtgcagc tgcccggcta ctactacgtg gactccaagc tggacatcac ctcccacaac
     4441 gaggactaca ccatcgtgga gcagtacgag cgcgccgagg gccgccacca cctgttcctg
     4501 tagcggccgg ccgcgactct agatcataat cagccatacc aTatttgtag aggttttact
     4561 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     4621 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4681 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4741 tgtatcttaa tcgagggggg gcccggtacc caattcgccc tatagtgagt CTCGAGccaa
     4801 gtgcctcaat tcgatcaatc aacgattaaa aatattgagt ctgcaaaagg acttgatgtg
     4861 tgggacagct ggccgctgca aaacgctgac ggaacagtag ctgaatacaa cggctatcac
     4921 gttgtgtttg ctcttgcggg aagcccgaaa gacgctgatg acacatcaat ctacatgttt
     4981 tatcaaaagg tcggcgacaa ctcaatcgac agctggaaaa acgcgggccg tgtctttaCT
     5041 CGAGgggggg cccggtacCA GCTTTTGTTC CCTTTAGTGA GGGTTAATTG CGCGCTTGGC
     5101 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
     5161 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
     5221 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
     5281 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC
     5341 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
     5401 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
     5461 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
     5521 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     5581 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     5641 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     5701 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     5761 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     5821 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     5881 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     5941 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     6001 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     6061 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     6121 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     6181 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     6241 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     6301 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     6361 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG
     6421 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     6481 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     6541 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     6601 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     6661 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     6721 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     6781 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     6841 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     6901 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     6961 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     7021 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     7081 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     7141 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     7201 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCAC
//

