LOCUS pBS-TarHom-Tol2- 7302 bp DNA linear 01-MAR-2016 FEATURES Location/Qualifiers misc_feature complement(5166..5189) /note="M13Rev" misc_feature complement(5112..5131) /note="T3" misc_feature 94..109 /note="M13F(-20)" misc_feature 120..139 /note="T7" misc_feature 399..1218 /note="Tol2 Exon4" misc_feature 153..382 /note="5 prime arm of homology for pTARBAC" misc_feature 153..173 /note="forward primer for amplification of targeting cassette" misc_feature 2546..3099 /note="Tol2 Exon1" misc_feature 3106..3825 /note="cryaa promoter" misc_feature 3826..4545 /note="sfGFP" misc_feature 4833..5080 /note="3 prime arm of homology for pTARBAC" misc_feature 4607..4791 /note="SV40 polyA" misc_feature complement(5060..5080) /note="rev primer for amplification of targeting cassette" misc_feature 1225..2539 /note="kana resistance cassette" source 1..7302 /dnas_title="pBS-TarHom-Tol2-FRT-GalK-cryaa-sfGFP-correcte d" ORIGIN 1 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT 61 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT 121 AATACGACTC ACTATAGGGC GAATTGGAGC TCggcggccg ccaggcctac ccactagtca 181 attcgggagg atcgaaacgg cagatcgcaa aaaacagtac atacagaagg agacatgaac 241 atgaacatca aaaaaattgt aaaacaagcc acagttctga cttttacgac tgcacttctg 301 gcaggaggag cgactcaagc cttcgcgaaa gaaaataacc aaaaagcata caaagaaacg 361 tacgcgtctc tcatattaca cgCCGCGGTG GCGGCCGCAT CTGGCCTTGT TTCAGACACC 421 AGGGAGTCTC TGCTCACGTT TCCTGCTATT TGCAGCCTCT CTATCAAGAC TAATACACCT 481 CTTCCCGCAT CGGCTGCCTG TGAGAGGCTT TTCAGCACTG CGGGATTGCT TTTCAGCCCC 541 AAAAGAGCTA GGCTTGACAC TAACAATTTT GAGAATCAGC TTCTACTGAA GTTAAATCTG 601 AGGTTTTACA ACTTTGAGTA GCGTGTACTG GCgTTAGATT GTCTGTCTTA TAGTTTGATA 661 ATTAAATACA AACAGTTCTA AAGCAGGATA AAACCTTGTA TGCATTTCAT TTAATGTTTT 721 TTGAGATTAA AAGCTTAAAC AAGAATCTCT AGTTTTCTTT CTTGCTTTTA CTTTTACTTC 781 CTTAATACTC AAGTACAATT TTAATGGAGT ACTTTTTTAC TTTTACTCAA GTAAGATTCT 841 AGCCAGATAC TTTTACTTTT AATTGAGTAA AATTTTCCCT AAGTACTTGT ACTTTCACTT 901 GAGTAAAATT TTTGAGTACT TTTTACACCT CTGTCAAGAA CTCCTGGACA AACCTCTGAC 961 CTGTGTGGAA CAGAGTGGAT ATGGGTGTCT GAACAGATAT TCACGTCTTT TGCAGATCAG 1021 AGGGCATTTC TGGTGTTGTG CAATGACCTG GGTCCAACAG GTAGGCGTAC TCGTATCCAA 1081 GAGCCTTGTT GGACAGGAGG TAGTCTCCAT TCCTATAAAG AGGAAGGAAT GGCACCATGT 1141 AGTAGCCGTC ATTGTGGCCA ATTGGTGCAT TGGTACGTGG GTAGATGGAC CGGGGAGGCT 1201 GATGAGTTCT TAGCCAGCGG ATCTgattac cctgttatcc ctagatttaa atgatatcgg 1261 atcctagtaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat 1321 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg 1381 agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct 1441 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat 1501 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt 1561 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt 1621 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc 1681 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt 1741 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt 1801 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt 1861 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa 1921 atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt 1981 gataacctta tttttgacga ggggGaatta aCaggttgta ttgatgttgg acgagtcgga 2041 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct 2101 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg 2161 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac 2221 tggcagagca ttacgctgac ttgacgggac ggcggctttg ttgaataaat cgaactcttg 2281 ctgagttgaa ggatcGgatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc 2341 aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc 2401 tcactttctg gctggATGAT Ggggcgattc aggcctggta tgagtcagca acaccttctt 2461 cacgaggcag acctcagcgg cggccggatc cgatatcgtt tggcgcgcca aactcgctac 2521 cttaggaccg ttatagttaA GATCCGATCT GCTGGGCTTG CTGAAGGTAG GGGGTCAAGA 2581 ACCAGAGGTG TAAAGTACTT GAGTAATTTT ACTTGATTAC TGTACTTAAG TATTATTTTT 2641 GGGGATTTTT ACTTTACTTG AGTACAATTA AAAATCAATA CTTTTACTTT TACTTAATTA 2701 CATTTTTTTA GAAAAAAAAG TACTTTTTAC TCCTTACAAT TTTATTTACA GTCAAAAAGT 2761 ACTTATTTTT TGGAGATCAC TTCATTCTAT TTTCCCTTGC TATTACCAAA CCAATTGAAT 2821 TGCGCTGATG CCCAGTTTAA TTTAAATGTT ATTTATTCTG CCTATGAAAA TCGTTTTCAC 2881 ATTATATGAA ATTGGTCAGA CATGTTCATT GGTCCTTTGG AAGTGACGTC ATGTCACATC 2941 TATTACCACA ATGCACAGCA CCTTGACCTG GAAATTAGGG AAATTATAAC AGTCAATCAG 3001 TGGAAGAAAA TGGAGGAAGT ATGTGATTCA TCAGCAGCTG CGAGCAGCAC AGTCCAAAAT 3061 CAGCCACAGG ATCAAGAGCA CCCGTGGCCG TATCTTCGCC CCGGGagctt cgaattctaa 3121 tatggcaata aacgatcttc agagaaactt gcattggtaa atagtttcag cctatgcata 3181 tgtagttatt caaactgtaa tgactatctt acagtattat atgtttataa tagtttgatc 3241 caataaatcc agatttatat gcttttcaac ataaaattgt gaaatgcaca gaatgtacat 3301 atacaataca tttcctacaa taatgctctc ttttctatta atagtgtgca ttcagtgcag 3361 gatgttatgt atttattttc tgtagtgcaa actttttcaa agaattttcc agtctaagct 3421 tttcagtcaa gaaaaaaaaa agcgattgat tatctgtgat atttaaaaaa attagctctt 3481 aaatctggtg aaccaagtgg ccacaacaca ttaaatactg acgttctaca gccatgtagc 3541 ttcatctggt ctggtttgtt ttggcaggca cttattttcc ggtgatagtt ggccacaaga 3601 gtattgtctg agccattcag tgctagactg tcattctcag gtcagagtcc accccgctga 3661 ttctgctgac aacactgttc ccaccatgag ataatgccat tccagagaga tccatttgta 3721 agcccctctt tctgcagcac aggtatataa ccaggggtct gcctccacta aggccggcac 3781 acatcatttg gggatctttg tactgtggat ccaccggtcg ccaccATGGT GAGCAAGGGC 3841 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC 3901 CACAAGTTCA GCGTGCGCGG CGAGGGCGAG GGCGATGCCA CCAACGGCAA GCTGACCCTG 3961 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCtTGGC CCACCCTCGT GACCACCCTG 4021 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCGCCA CGACTTCTTC 4081 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCAGCTTCAA GGACGACGGC 4141 ACCTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG 4201 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC 4261 TTCAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC 4321 TTCAAGATCC GCCACAACGT GGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG 4381 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG 4441 TCCGTGCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTgct GGAgttCGTG 4501 ACCGCCGCCG GGATCACTCA CGGCATGGAC GAGCTGTACA AGTAGcggcc ggccgcgact 4561 ctagatcata atcagccata ccaTatttgt agaggtttta cttgctttaa aaaacctccc 4621 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat 4681 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 4741 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aatcgagggg 4801 gggcccggta cccaattcgc cctatagtga gtCTCGAGcc aagtgcctca attcgatcaa 4861 tcaacgatta aaaatattga gtctgcaaaa ggacttgatg tgtgggacag ctggccgctg 4921 caaaacgctg acggaacagt agctgaatac aacggctatc acgttgtgtt tgctcttgcg 4981 ggaagcccga aagacgctga tgacacatca atctacatgt tttatcaaaa ggtcggcgac 5041 aactcaatcg acagctggaa aaacgcgggc cgtgtcttta CTCGAGgggg ggcccggtac 5101 CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC 5161 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 5221 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 5281 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 5341 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 5401 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 5461 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 5521 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 5581 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 5641 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 5701 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 5761 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 5821 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 5881 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 5941 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG 6001 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 6061 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 6121 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 6181 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 6241 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 6301 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 6361 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 6421 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 6481 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 6541 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 6601 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 6661 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 6721 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 6781 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 6841 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 6901 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 6961 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 7021 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 7081 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 7141 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 7201 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 7261 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC AC //