LOCUS       pBS-TarHom-Tol2-        7302 bp    DNA     linear       01-MAR-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(5166..5189)
                     /note="M13Rev"
     misc_feature    complement(5112..5131)
                     /note="T3"
     misc_feature    94..109
                     /note="M13F(-20)"
     misc_feature    120..139
                     /note="T7"
     misc_feature    399..1218
                     /note="Tol2 Exon4"
     misc_feature    153..382
                     /note="5 prime arm of homology for pTARBAC"
     misc_feature    153..173
                     /note="forward primer for amplification of targeting
                     cassette"
     misc_feature    2546..3099
                     /note="Tol2 Exon1"
     misc_feature    3106..3825
                     /note="cryaa promoter"
     misc_feature    3826..4545
                     /note="sfGFP"
     misc_feature    4833..5080
                     /note="3 prime arm of homology for pTARBAC"
     misc_feature    4607..4791
                     /note="SV40 polyA"
     misc_feature    complement(5060..5080)
                     /note="rev primer for amplification of targeting cassette"
     misc_feature    1225..2539
                     /note="kana resistance cassette"
     source          1..7302
                     /dnas_title="pBS-TarHom-Tol2-FRT-GalK-cryaa-sfGFP-correcte
                     d"
ORIGIN      
        1 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT
       61 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT
      121 AATACGACTC ACTATAGGGC GAATTGGAGC TCggcggccg ccaggcctac ccactagtca
      181 attcgggagg atcgaaacgg cagatcgcaa aaaacagtac atacagaagg agacatgaac
      241 atgaacatca aaaaaattgt aaaacaagcc acagttctga cttttacgac tgcacttctg
      301 gcaggaggag cgactcaagc cttcgcgaaa gaaaataacc aaaaagcata caaagaaacg
      361 tacgcgtctc tcatattaca cgCCGCGGTG GCGGCCGCAT CTGGCCTTGT TTCAGACACC
      421 AGGGAGTCTC TGCTCACGTT TCCTGCTATT TGCAGCCTCT CTATCAAGAC TAATACACCT
      481 CTTCCCGCAT CGGCTGCCTG TGAGAGGCTT TTCAGCACTG CGGGATTGCT TTTCAGCCCC
      541 AAAAGAGCTA GGCTTGACAC TAACAATTTT GAGAATCAGC TTCTACTGAA GTTAAATCTG
      601 AGGTTTTACA ACTTTGAGTA GCGTGTACTG GCgTTAGATT GTCTGTCTTA TAGTTTGATA
      661 ATTAAATACA AACAGTTCTA AAGCAGGATA AAACCTTGTA TGCATTTCAT TTAATGTTTT
      721 TTGAGATTAA AAGCTTAAAC AAGAATCTCT AGTTTTCTTT CTTGCTTTTA CTTTTACTTC
      781 CTTAATACTC AAGTACAATT TTAATGGAGT ACTTTTTTAC TTTTACTCAA GTAAGATTCT
      841 AGCCAGATAC TTTTACTTTT AATTGAGTAA AATTTTCCCT AAGTACTTGT ACTTTCACTT
      901 GAGTAAAATT TTTGAGTACT TTTTACACCT CTGTCAAGAA CTCCTGGACA AACCTCTGAC
      961 CTGTGTGGAA CAGAGTGGAT ATGGGTGTCT GAACAGATAT TCACGTCTTT TGCAGATCAG
     1021 AGGGCATTTC TGGTGTTGTG CAATGACCTG GGTCCAACAG GTAGGCGTAC TCGTATCCAA
     1081 GAGCCTTGTT GGACAGGAGG TAGTCTCCAT TCCTATAAAG AGGAAGGAAT GGCACCATGT
     1141 AGTAGCCGTC ATTGTGGCCA ATTGGTGCAT TGGTACGTGG GTAGATGGAC CGGGGAGGCT
     1201 GATGAGTTCT TAGCCAGCGG ATCTgattac cctgttatcc ctagatttaa atgatatcgg
     1261 atcctagtaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1321 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     1381 agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct
     1441 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     1501 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     1561 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     1621 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     1681 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     1741 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     1801 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     1861 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     1921 atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     1981 gataacctta tttttgacga ggggGaatta aCaggttgta ttgatgttgg acgagtcgga
     2041 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2101 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2161 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     2221 tggcagagca ttacgctgac ttgacgggac ggcggctttg ttgaataaat cgaactcttg
     2281 ctgagttgaa ggatcGgatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc
     2341 aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc
     2401 tcactttctg gctggATGAT Ggggcgattc aggcctggta tgagtcagca acaccttctt
     2461 cacgaggcag acctcagcgg cggccggatc cgatatcgtt tggcgcgcca aactcgctac
     2521 cttaggaccg ttatagttaA GATCCGATCT GCTGGGCTTG CTGAAGGTAG GGGGTCAAGA
     2581 ACCAGAGGTG TAAAGTACTT GAGTAATTTT ACTTGATTAC TGTACTTAAG TATTATTTTT
     2641 GGGGATTTTT ACTTTACTTG AGTACAATTA AAAATCAATA CTTTTACTTT TACTTAATTA
     2701 CATTTTTTTA GAAAAAAAAG TACTTTTTAC TCCTTACAAT TTTATTTACA GTCAAAAAGT
     2761 ACTTATTTTT TGGAGATCAC TTCATTCTAT TTTCCCTTGC TATTACCAAA CCAATTGAAT
     2821 TGCGCTGATG CCCAGTTTAA TTTAAATGTT ATTTATTCTG CCTATGAAAA TCGTTTTCAC
     2881 ATTATATGAA ATTGGTCAGA CATGTTCATT GGTCCTTTGG AAGTGACGTC ATGTCACATC
     2941 TATTACCACA ATGCACAGCA CCTTGACCTG GAAATTAGGG AAATTATAAC AGTCAATCAG
     3001 TGGAAGAAAA TGGAGGAAGT ATGTGATTCA TCAGCAGCTG CGAGCAGCAC AGTCCAAAAT
     3061 CAGCCACAGG ATCAAGAGCA CCCGTGGCCG TATCTTCGCC CCGGGagctt cgaattctaa
     3121 tatggcaata aacgatcttc agagaaactt gcattggtaa atagtttcag cctatgcata
     3181 tgtagttatt caaactgtaa tgactatctt acagtattat atgtttataa tagtttgatc
     3241 caataaatcc agatttatat gcttttcaac ataaaattgt gaaatgcaca gaatgtacat
     3301 atacaataca tttcctacaa taatgctctc ttttctatta atagtgtgca ttcagtgcag
     3361 gatgttatgt atttattttc tgtagtgcaa actttttcaa agaattttcc agtctaagct
     3421 tttcagtcaa gaaaaaaaaa agcgattgat tatctgtgat atttaaaaaa attagctctt
     3481 aaatctggtg aaccaagtgg ccacaacaca ttaaatactg acgttctaca gccatgtagc
     3541 ttcatctggt ctggtttgtt ttggcaggca cttattttcc ggtgatagtt ggccacaaga
     3601 gtattgtctg agccattcag tgctagactg tcattctcag gtcagagtcc accccgctga
     3661 ttctgctgac aacactgttc ccaccatgag ataatgccat tccagagaga tccatttgta
     3721 agcccctctt tctgcagcac aggtatataa ccaggggtct gcctccacta aggccggcac
     3781 acatcatttg gggatctttg tactgtggat ccaccggtcg ccaccATGGT GAGCAAGGGC
     3841 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
     3901 CACAAGTTCA GCGTGCGCGG CGAGGGCGAG GGCGATGCCA CCAACGGCAA GCTGACCCTG
     3961 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCtTGGC CCACCCTCGT GACCACCCTG
     4021 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCGCCA CGACTTCTTC
     4081 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCAGCTTCAA GGACGACGGC
     4141 ACCTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
     4201 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
     4261 TTCAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC
     4321 TTCAAGATCC GCCACAACGT GGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
     4381 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
     4441 TCCGTGCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTgct GGAgttCGTG
     4501 ACCGCCGCCG GGATCACTCA CGGCATGGAC GAGCTGTACA AGTAGcggcc ggccgcgact
     4561 ctagatcata atcagccata ccaTatttgt agaggtttta cttgctttaa aaaacctccc
     4621 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     4681 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4741 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aatcgagggg
     4801 gggcccggta cccaattcgc cctatagtga gtCTCGAGcc aagtgcctca attcgatcaa
     4861 tcaacgatta aaaatattga gtctgcaaaa ggacttgatg tgtgggacag ctggccgctg
     4921 caaaacgctg acggaacagt agctgaatac aacggctatc acgttgtgtt tgctcttgcg
     4981 ggaagcccga aagacgctga tgacacatca atctacatgt tttatcaaaa ggtcggcgac
     5041 aactcaatcg acagctggaa aaacgcgggc cgtgtcttta CTCGAGgggg ggcccggtac
     5101 CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC
     5161 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA
     5221 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT
     5281 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC
     5341 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
     5401 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
     5461 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
     5521 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG
     5581 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
     5641 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
     5701 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT
     5761 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
     5821 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
     5881 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
     5941 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG
     6001 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
     6061 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
     6121 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
     6181 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA
     6241 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
     6301 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT
     6361 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT
     6421 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT
     6481 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT
     6541 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
     6601 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG
     6661 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT
     6721 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG
     6781 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG
     6841 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
     6901 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA
     6961 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC
     7021 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT
     7081 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG
     7141 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
     7201 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA
     7261 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC AC
//

