LOCUS       pBS-TarHom-Tol2-        7301 bp    DNA     linear       01-MAR-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(5165..5188)
                     /note="M13Rev"
     misc_feature    complement(5111..5130)
                     /note="T3"
     misc_feature    94..109
                     /note="M13F(-20)"
     misc_feature    120..139
                     /note="T7"
     misc_feature    399..1218
                     /note="Tol2 Exon4"
     misc_feature    153..382
                     /note="5 prime arm of homology for pTARBAC"
     misc_feature    153..173
                     /note="forward primer for amplification of targeting
                     cassette"
     misc_feature    2545..3098
                     /note="Tol2 Exon1"
     misc_feature    3105..3824
                     /note="cryaa promoter"
     misc_feature    3825..4543
                     /note="Citrine"
     misc_feature    4832..5079
                     /note="3 prime arm of homology for pTARBAC"
     misc_feature    4606..4790
                     /note="SV40 polyA"
     misc_feature    complement(5059..5079)
                     /note="rev primer for amplification of targeting cassette"
     misc_feature    1225..2538
                     /note="kana resistance cassette"
     source          1..7301
                     /dnas_title="pBS-TarHom-Tol2-FRT-GalK-cryaa-YFP-corrected"
ORIGIN      
        1 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT
       61 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT
      121 AATACGACTC ACTATAGGGC GAATTGGAGC TCggcggccg ccaggcctac ccactagtca
      181 attcgggagg atcgaaacgg cagatcgcaa aaaacagtac atacagaagg agacatgaac
      241 atgaacatca aaaaaattgt aaaacaagcc acagttctga cttttacgac tgcacttctg
      301 gcaggaggag cgactcaagc cttcgcgaaa gaaaataacc aaaaagcata caaagaaacg
      361 tacgcgtctc tcatattaca cgCCGCGGTG GCGGCCGCAT CTGGCCTTGT TTCAGACACC
      421 AGGGAGTCTC TGCTCACGTT TCCTGCTATT TGCAGCCTCT CTATCAAGAC TAATACACCT
      481 CTTCCCGCAT CGGCTGCCTG TGAGAGGCTT TTCAGCACTG CgGGATTGCT TTTCAGCCCC
      541 AAAAGAGCTA GGCTTGACAC TAACAATTTT GAGAATCAGC TTCTACTGAA GTTAAATCTG
      601 AGGTTTTACA ACTTTGAGTA GCGTGTACTG GCgTTAGATT GTCTGTCTTA TAGTTTGATA
      661 ATTAAATACA AACAGTTCTA AAGCAGGATA AAACCTTGTA TGCATTTCAT TTAATGTTTT
      721 TTGAGATTAA AAGCTTAAAC AAGAATCTCT AGTTTTCTTT CTTGCTTTTA CTTTTACTTC
      781 CTTAATACTC AAGTACAATT TTAATGGAGT ACTTTTTTAC TTTTACTCAA GTAAGATTCT
      841 AGCCAGATAC TTTTACTTTT AATTGAGTAA AATTTTCCCT AAGTACTTGT ACTTTCACTT
      901 GAGTAAAATT TTTGAGTACT TTTTACACCT CTGTCAAGAA CTCCTGGACA AACCTCTGAC
      961 CTGTGTGGAA CAGAGTGGAT ATGGGTGTCT GAACAGATAT TCACGTCTTT TGCAGATCAG
     1021 AGGGCATTTC TGGTGTTGTG CAATGACCTG GGTCCAACAG GTAGGCGTAC TCGTATCCAA
     1081 GAGCaTTGTT GGACAGGAGG TAGTCTCCAT TCCTATAAAG AGGAAGGAAT GGCACCATGT
     1141 AGTAGCCGTC ATTGTGGCCA ATTGGTGCAT TGGTACGTGG GTAGATGGAC CGGGGAGGCT
     1201 GATGAGTTCT TAGCCAGCGG ATCTgattac cctttatccc tagatttaaa tgatatcgga
     1261 tcctagtaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     1321 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     1381 gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg
     1441 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     1501 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     1561 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     1621 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     1681 ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     1741 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     1801 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     1861 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     1921 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     1981 ataaccttat ttttgacgag gggGaattaa Caggttgtat tgatgttgga cgagtcggaa
     2041 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     2101 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     2161 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact
     2221 ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc gaactcttgc
     2281 tgagttgaag gatcGgatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca
     2341 aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct
     2401 cactttctgg ctggATGATG gggcgattca ggcctggtat gagtcagcaa caccttcttc
     2461 acgaggcaga cctcagcggc ggccggatcc gatatcgttt ggcgcgccaa actcgctacc
     2521 ttaggaccgt tatagttaAG ATCCGATCTG CTGGGCTTGC TGAAGGTAGG GGGTCAAGAA
     2581 CCAGAGGTGT AAAGTACTTG AGTAATTTTA CTTGATTACT GTACTTAAGT ATTATTTTTG
     2641 GGGATTTTTA CTTTACTTGA GTACAATTAA AAATCAATAC TTTTACTTTT ACTTAATTAC
     2701 ATTTTTTTAG AAAAAAAAGT ACTTTTTACT CCTTACAATT TTATTTACAG TCAAAAAGTA
     2761 CTTATTTTTT GGAGATCACT TCATTCTATT TTCCCTTGCT ATTACCAAAC CAATTGAATT
     2821 GCGCTGATGC CCAGTTTAAT TTAAATGTTA TTTATTCTGC CTATGAAAAT CGTTTTCACA
     2881 TTATATGAAA TTGGTCAGAC ATGTTCATTG GTCCTTTGGA AGTGACGTCA TGTCACATCT
     2941 ATTACCACAA TGCACAGCAC CTTGACCTGG AAATTAGGGA AATTATAtCA GTCAATCAGT
     3001 GGAAGAAAAT GGAGGAAGTA TGTGATTCAT CAGCAGCTGC GAGCAGCACA GTCCAAAATC
     3061 AGCCACAGGA TCAAGAGCAC CCGTGGCCGT ATCTTCGCCC CGGGagcttc gaattctaat
     3121 atggcaataa acgatcttca gagaaacttg cattggtaaa tagtttcagc ctatgcatat
     3181 gtagttattc aaactgtaat gactatctta cagtattata tgtttataat agtttgatcc
     3241 aataaatcca gatttatatg cttttcaaca taaaattgtg aaatgcacag aatgtacata
     3301 tacaatacat ttcctacaat aatgctctct tttctattaa tagtgtgcat tcagtgcagg
     3361 atgttatgta tttattttct gtagtgcaaa ctttttcaaa gaattttcca gtctaagctt
     3421 ttcagtcaag aaaaaaaaaa gcgattgatt atctgtgata tttaaaaaaa ttagctctta
     3481 aatctggtga accaagtggc cacaacacat taaatactga cgttctacag ccatgtagct
     3541 tcatctggtc tggtttgctt tggcaggcac ttattttccg gtgatagttg gccacaagag
     3601 tattgtctga gccattcagt gctagactgt cattctcagg tcagagtcca ccccgctgat
     3661 tctgctgaca acactgttcc caccatgaga taatgccatt ccagagagat ccatttgtaa
     3721 gcccctcttt ctgcagcaca ggtatataac caggggtctg cctccactaa ggccggcaca
     3781 catcatttgg ggatctttgt actgtggatc caccggtcgc caccatggtg agcaagggcg
     3841 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     3901 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     3961 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     4021 gctacggcct gatgtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     4081 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     4141 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     4201 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     4261 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     4321 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     4381 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     4441 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4501 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gTAGcggccg gccgcgactc
     4561 tagatcataa tcagccatac catatttgta gaggttttac ttgctttaaa aaacctccca
     4621 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     4681 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4741 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta atcgaggggg
     4801 ggcccggtac ccaattcgcc ctatagtgag tCTCGAGcca agtgcctcaa ttcgatcaat
     4861 caacgattaa aaatattgag tctgcaaaag gacttgatgt gtgggacagc tggccgctgc
     4921 aaaacgctga cggaacagta gctgaataca acggctatca cgttgtgttt gctcttgcgg
     4981 gaagcccgaa agacgctgat gacacatcaa tctacatgtt ttatcaaaag gtcggcgaca
     5041 actcaatcga cagctggaaa aacgcgggcc gtgtctttaC TCGAGggggg gcccggtacC
     5101 CAGCTTTTGT TCCCTTTAGT GAGGGTTAAT TGCGCGCTTG GCGTAATCAT GGTCATAGCT
     5161 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT
     5221 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC
     5281 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
     5341 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
     5401 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     5461 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     5521 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     5581 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     5641 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     5701 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     5761 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     5821 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     5881 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     5941 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
     6001 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     6061 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     6121 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     6181 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     6241 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     6301 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     6361 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     6421 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT
     6481 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     6541 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     6601 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     6661 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     6721 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     6781 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     6841 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     6901 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     6961 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     7021 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     7081 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     7141 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
     7201 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
     7261 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA C
//

