LOCUS       Exported                8696 bp ds-DNA     circular SYN 02-MAY-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    74449
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 2, 2016 from SnapGene 3.1.2
            http://www.snapgene.com
COMMENT     [hS4c-ectodom.txt.xdna - 1989 bp] Fragment extracted from p 
            CRII-hS4C-ectodom.xdna [between344and 2332] -  -  - Plasmid 74449: 
            pEF-hSEMA4C-Fc- Our sequencing results using primer G -CSF-R 
            [5'-GATGGGGAACACTGCTGTTTA-3'] found 4 single nucleoti de mismatches 
            in the human Fc tag.  - When compared to the p rovided annotated 
            Genbank file, the mismatches were located at bp6792 (T>G), bp6796 
            (C>A), bp6945 (C>T), and bp7056 (T>C ). I was able to find one 
            Genbank reference seqeunce that do es contain these mutations- 
            ABI97155.1. -
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..3736
                     /note="source [*]"
                     /note="organism=""pEF-BOS"" - mol_type=""other DNA"" - 
                     1..5685"
     source          7782..8696
                     /note="source [*] [3']"
                     /note="organism=""pEF-BOS"" - mol_type=""other DNA"" - 
                     1..5685"
     promoter        131..159
                     /note="AmpR_promoter"
                     /note="label=""AmpR_promoter"" - 131..159"
     gene            201..1061
                     /note="Ampicillin_Ampicillin"
                     /note="label=""Ampicillin"" - gene=""Ampicillin"" - 
                     201..1061"
     CDS             201..1061
                     /note="ORF frame 3_Ampicillin_Ampicillin"
                     /note="label=""Ampicillin"" - gene=""Ampicillin"" - 
                     note=""ORF frame 3"" - 
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY 
                     - 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     - 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     - DRWEPELNEAI"
     rep_origin      1216..1835
                     /direction=RIGHT
                     /note="pBR322_origin"
                     /note="label=""pBR322_origin"" - 1216..1835"
     promoter        2144..2173
                     /note="lac_promoter"
                     /note="label=""lac_promoter"" - 2144..2173"
     misc_feature    2187..2209
                     /note="M13_pUC_rev_primer"
                     /note="label=""M13_pUC_rev_primer"" - 2187..2209"
     misc_feature    2208..2226
                     /note="M13_reverse_primer"
                     /note="label=""M13_reverse_primer"" - 2208..2226"
     promoter        2273..2475
                     /note="SV40_promoter"
                     /note="label=""SV40_promoter"" - 2273..2475"
     rep_origin      2320..2397
                     /direction=RIGHT
                     /note="SV40_origin"
                     /note="label=""SV40_origin"" - 2320..2397"
     misc_feature    2382..2401
                     /note="SV40pro_F_primer"
                     /note="label=""SV40pro_F_primer"" - 2382..2401"
     promoter        2553..3736
                     /note="EF1a_promoter"
                     /note="label=""EF1a_promoter"" - 2553..3736"
     CDS             complement(2856..3476)
                     /note="ORF frame 3"
                     /note="label=""ORF frame 3"" - 
                     translation=""MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL 
                     - 
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP 
                     - 
                     RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR 
                     - GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*"" -"
     primer_bind     3679..3702
                     /note="pEF-F"
     misc_feature    3684..3704
                     /note="EF1a_fwd_primer"
                     /note="label=""EF1a_fwd_primer"" - 3684..3704"
     misc_feature    3737..3740
                     /note="F sequence read [5'] [*] [5']"
     misc_feature    3742..3806
                     /note="F sequence read [5'] [*] [3']"
     misc_feature    3806
                     /note="sequence read seq2 [5'] [*]"
     misc_feature    3808..5790
                     /note="hS4c ectodom"
     misc_feature    5795..5817
                     /note="intron synthetic"
                     /note="note=""direct strand 
                     translation"";translation=""VSRIH*"""
     misc_feature    5818..7068
                     /note="Fc portion"
     misc_feature    5818..6155
                     /note="intron"
     misc_feature    6158
                     /note="frame 1st base"
                     /note="note=""direct strand 
                     translation"";translation=""X"""
     misc_feature    6201..6318
                     /note="intron"
     misc_feature    6321
                     /note="frame 1st base"
                     /note="note=""direct strand 
                     translation"";translation=""X"""
     misc_feature    6649..6745
                     /note="intron"
     misc_feature    6748
                     /note="frame 1st base"
                     /note="note=""direct strand 
                     translation"";translation=""X"""
     misc_feature    7833..7988
                     /note="lacZ_a"
                     /note="label=""lacZ_a"" - 4812..4967"
     misc_feature    complement(7836..7852)
                     /note="M13_forward20_primer"
                     /note="label=""M13_forward20_primer"" - 
                     complement(4815..4831)"
     misc_feature    complement(7845..7867)
                     /note="M13_pUC_fwd_primer"
                     /note="label=""M13_pUC_fwd_primer"" - 
                     complement(4824..4846)"
     rep_origin      complement(8177..8483)
                     /direction=LEFT
                     /note="f1_origin"
                     /note="label=""f1_origin"" - complement(5156..5462)"
     misc_feature    8656..8678
                     /note="pGEX_3_primer"
                     /note="label=""pGEX_3_primer"" - 5635..5657"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2281 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     2341 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2401 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     2461 ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     2521 aatggacctt ctaggtcttg aaaggagtgc ctcgtgaggc tccggtgccc gtcagtgggc
     2581 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     2641 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     2701 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     2761 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     2821 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     2881 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     2941 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     3001 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     3061 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     3121 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     3181 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     3241 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     3301 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     3361 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     3421 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     3481 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     3541 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     3601 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     3661 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     3721 atttcaggtg tcgtgaggaa ttctctagag atccctcgac ctcgagatcc atcgggcccc
     3781 ccctcgaggt cgactcgcct ctacctgatg gccccacact gggctgtctg gctgctggca
     3841 gcaaggctgt ggggcctggg cattggggct gaggtgtggt ggaaccttgt gccgcgtaag
     3901 acagtgtctt ctggggagct ggccacggta gtacggcggt tctcccagac cggcatccag
     3961 gacttcctga cactgacgct gacggagccc actgggcttc tgtacgtggg cgcccgagag
     4021 gccctgtttg ccttcagcat ggaggccctg gagctgcaag gagcgatctc ctgggaggcc
     4081 cccgtggaga agaagactga gtgtatccag aaagggaaga acaaccagac cgagtgcttc
     4141 aacttcatcc gcttcctgca gccctacaat gcctcccacc tgtacgtctg tggcacctac
     4201 gccttccagc ccaagtgcac ctacgtcaac atgctcacct tcactttgga gcatggagag
     4261 tttgaagatg ggaagggcaa gtgtccctat gacccagcta agggccatgc tggccttctt
     4321 gtggatggtg agctgtactc ggccacactc aacaacttcc tgggcacgga acccattatc
     4381 ctgcgtaaca tggggcccca ccactccatg aagacagagt acctggcctt ttggctcaac
     4441 gaacctcact ttgtaggctc tgcctatgta cctgagagtg tgggcagctt cacgggggac
     4501 gacgacaagg tctacttctt cttcagggag cgggcagtgg agtccgactg ctatgccgag
     4561 caggtggtgg ctcgtgtggc ccgtgtctgc aagggcgata tggggggcgc acggaccctg
     4621 cagaggaagt ggaccacgtt cctgaaggcg cggctggcat gctctgcccc gaactggcag
     4681 ctctacttca accagctgca ggcgatgcac accctgcagg acacctcctg gcacaacacc
     4741 accttctttg gggtttttca agcacagtgg ggtgacatgt acctgtcggc catctgtgag
     4801 taccagttgg aagagatcca gcgggtgttt gagggcccct ataaggagta ccatgaggaa
     4861 gcccagaagt gggaccgcta cactgaccct gtacccagcc ctcggcctgg ctcgtgcatt
     4921 aacaactggc atcggcgcca cggctacacc agctccctgg agctacccga caacatcctc
     4981 aacttcgtca agaagcaccc gctgatggag gagcaggtgg ggcctcggtg gagccgcccc
     5041 ctgctcgtga agaagggcac caacttcacc cacctggtgg ccgaccgggt tacaggactt
     5101 gatggagcca cctatacagt gctgttcatt ggcacaggag acggctggct gctcaaggct
     5161 gtgagcctgg ggccctgggt tcacctgatt gaggagctgc agctgtttga ccaggagccc
     5221 atgagaagcc tggtgctatc tcagagcaag aagctgctct ttgccggctc ccgctctcag
     5281 ctggtgcagc tgcccgtggc cgactgcatg aagtatcgct cctgtgcaga ctgtgtcctc
     5341 gcccgggacc cctattgcgc ctggagcgtc aacaccagcc gctgtgtggc cgtgggtggc
     5401 cactctggat ctctactgat ccagcatgtg atgacctcgg acacttcagg catctgcaac
     5461 ctccgtggca gtaagaaagt caggcccact cccaaaaaca tcacggtggt ggcgggcaca
     5521 gacctggtgc tgccctgcca cctctcctcc aacttggccc atgcccgctg gacctttggg
     5581 ggccgggacc tgcctgcgga acagcccggg tccttcctct acgatgcccg gctccaggcc
     5641 ctggttgtga tggctgccca gccccgccat gccggggcct accactgctt ttcagaggag
     5701 cagggggcgc ggctggctgc tgaaggctac cttgtggctg tcgtggcagg cccgtcggtg
     5761 accttggagg cccgggcccc cctggaaaac caaagtaagt aggatccact agttctatcc
     5821 tgcctggacg catcccggct atgcagtccc agtccagggc agcaaggcag gccccgtctg
     5881 cctcttcacc cggaggcctc tgcccgcccc actcatgctc agggagaggg tcttctggct
     5941 ttttccccag gctctgggca ggcacaggct aggtgcccct aacccaggcc ctgcacacaa
     6001 aggggcaggt gctgggctca gacctgccaa gagccatatc cgggaggacc ctgcccctga
     6061 cctaagccca ccccaaaggc caaactctcc actccctcag ctcggacacc ttctctcctc
     6121 ccagattcca gtaactccca atcttctctc tgcagagccc aaatcttgtg acaaaactca
     6181 cacatgccca ccgtgcccag gtaagccagc ccaggcctcg ccctccagct caaggcggga
     6241 caggtgccct agagtagcct gcatccaggg acaggcccca gccgggtgct gacacgtcca
     6301 cctccatctc ttcctcagca cctgaactcc tggggggacc gtcagtcttc ctcttccccc
     6361 caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc gtggtggtgg
     6421 acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc gtggaggtgc
     6481 ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt gtggtcagcg
     6541 tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc aaggtctcca
     6601 acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggt gggacccgtg
     6661 gggtgcgagg gccacatgga cagaggccgg ctcggcccac cctctgccct gagagtgacc
     6721 gctgtaccaa cctctgtccc tacagggcag ccccgagaac cacaggtgta caccctgccc
     6781 ccatcccggg aggagatgac caagaaccag gtcagcctga cctgcctggt caaaggcttc
     6841 tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag
     6901 accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctatagcaa gctcaccgtg
     6961 gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg
     7021 cacaaccact acacgcagaa gagcctctcc ctgtccccgg gtaaatgagt gcgacggccg
     7081 gcaagccccc gctccccggg ctctcgcggt cgcacgagga tgcttggcac gtaccccgtc
     7141 tacatacttc ccaggcaccc agcatggaaa taaagcaccc accactgccc tagagcggcc
     7201 gcgactctag agtgagggtc cccacctggg acccttgaga gtatcaggtc tcccacgtgg
     7261 gagacaagaa atccctgttt aatatttaaa cagcagtgtt ccccatctgg gtccttgcac
     7321 ccctcactct ggcctcagcc gactgcacag cggcccctgc atccccttgg ctgtgaggcc
     7381 cctggacaag cagaggtggc cagagctggg aggcatggcc ctggggtccc acgaatttgc
     7441 tggggaatct cgtttttctt cttaagactt ttgggacatg gtttgactcc cgaacatcac
     7501 cgacgcgtct cctgtttttc tgggtggcct cgggacacct gccctgcccc cacgagggtc
     7561 aggactgtga ctctttttag ggccaggcag gtgcctggac atttgccttg ctggacgggg
     7621 actggggatg tgggagggag cagacaggag gaatcatgtc aggcctgtgt gtgaaaggaa
     7681 gctccactgt caccctccac ctcttcaccc cccactcacc agtgtcccct ccactgtcac
     7741 attgtaactg aacttcagga taataaagtg cttgcctcca aaaaaaaaaa aaaaaaaaaa
     7801 aaaaaaaaaa aaaaaaaaag aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7861 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7921 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7981 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaaaacgt
     8041 taatattttg ttaaaattcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
     8101 atggtggttc cgaaatcggc aaaatccctt ataaatcaaa agaatagccc gagatagggt
     8161 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     8221 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa
     8281 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     8341 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     8401 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     8461 ccgcgcttaa tgcgccgcta cagggcgcgt actatggttg ctttgacgag catggtgcac
     8521 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     8581 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     8641 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
