LOCUS       pDRBFC-B2-YN                10892 bp    DNA     linear       01-APR-2016
FEATURES             Location/Qualifiers
     misc_feature    2229..2649
                     /note="35S promoter"
     misc_feature    3652..4378
                     /note="OCS terminator"
     CDS             2737..2766
                     /note="HA"
                     /translation="MYPYDVPDYA"
     misc_feature    5..25
                     /note="LB"
     CDS             complement(373..924)
                     /note="Bar"
                     /translation=""
     misc_feature    4615..4639
                     /note="RB"
     CDS             2800..3018
                     /note="B2"
                     /translation="MPSKLALIQELPDRIQTAVEAAMGMSYQDAPNNVRRDLDNLHAC
                     LNKAKLTVSRMVTSLLEKPSVVAYLEGKA"
     CDS             3064..3585
                     /note="YN"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIED"
     CDS             2737..3585
                     /note="HA-B2-YN fusion protein"
                     /translation="MYPYDVPDYAITSLYKKAGCMMPSKLALIQELPDRIQTAVEAAM
                     GMSYQDAPNNVRRDLDNLHACLNKAKLTVSRMVTSLLEKPSVVAYLEGKADPAFLYKV
                     VPRSIATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT
                     GKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKT
                     RAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFK
                     IRHNIED"
     source          1..10892
                     /dnas_title=" pDRBFC-B2-YN vector"
ORIGIN      
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcgGATCT GATAATTTAT
      121 TTGAAAATTC ATAAGAAAAG CAAACGTTAC ATGAATTGAT GAAACAATAC AAAGACAGAT
      181 AAAGCCACGC ACATTTAGGA TATTGGCCGA GATTACTGAA TATTGAGTAA GATCACGGAA
      241 TTTCTGACAG GAGCATGTCT TCAATTCAGC CCAAATGGCA GTTGAAATAC TCAAACCGCC
      301 CCATATGCAG GAGCGGATCA TTCATTGTTT GTTTGGTTGC CTTTGCCAAC ATGGGAGTCC
      361 AAGATTCTGC AGTCAAATCT CGGTGACGGG CAGGACCGGA CGGGGCGGTA CCGGCAGGCT
      421 GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG
      481 CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG
      541 CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGGTG
      601 GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGGAGACGT ACACGGTCGA
      661 CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGG CCCGCGTAGG CGATGCCGGC
      721 GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC
      781 GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT
      841 GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC
      901 GGCCGGGCGT CGTTCTGGGC TCATCGATTC GATTTGGTGT ATCGAGATTG GTTATGAAAT
      961 TCAGATGCTA GTGTAATGTA TTGGTAATTT GGGAAGATAT AATAGGAAGC AAGGCTATTT
     1021 ATCCATTTCT GAAAAGGCGA AATGGCGTCA CCGCGAGCGT CACGCGCATT CCGTTCTTGC
     1081 TGTAAAGCGT TGTTTGGTAC ACTTTTGACT AGCGAGGCTT GGCGTGTCAG CGTATCTATT
     1141 CAAAAGTCGT TAATGGCTGC GGATCAAGAA AAAGTTGGAA TAGAAACAGA ATACCCGCGA
     1201 AATTCAGGCC CGGTTGCCAT GTCCTACACG CCGAAATAAA CGACCAAATT AGTAGAAAAA
     1261 TAAAAACTGA CTCGGATACT TACGTCACGT CTTGCGCACT GATTTGAAAA ATCTCAGaat
     1321 TCCAATCCCA CAAAAAtCTG AgCtTAaCAG caCAGTTGCT CCTCTCAGAG caGAATCGGG
     1381 TATTCAACAC CCTCATAtCA ACTACTACGT tGTGTATAAC GGtCCaCATG CCGGTATATA
     1441 CGATGACTGG GGTTGTACAA AGGCgGCAAC AAACGGcGTT CCCGGAGTTG CaCAcAAGAA
     1501 aTTTGCCACT ATTACAGAGG CAAGAGCAGC AGCTGACGCG TAcACAACAA GTCAGCAAAC
     1561 AGAcAGGTTG AACTTCATCC CCAAAGGAGA AGCTCAACTC AAGCCCAAGA GCTTTGCtAA
     1621 GGCCCTAACA AGCCCACCAA AGCAAAAAGC CCACTGgCTC ACGCTAGGAA CCAAAAGGCC
     1681 CAGCAGTGAT CCAGCCCCAA AAGAGAtCTC CTTTGCCCCG GAGATTACAA TGGACGATTT
     1741 CCTCTATCTT TACGATCTAG GAAGGAAGTT CGAAGGTGAA GGTGACGACA CTATGTTCAC
     1801 CACTGATAAT GAGAAGGTTA GCCTCTTCAA TTTCAGAAAG AATGCTGACC CACAGATGGT
     1861 TAGAGAGGCC TACGCAGCAG GTCTCATCAA GACGATCTAC CCGAGTAACA ATCTCCAGGA
     1921 GATCAAATAC CTTCCCAAGA AGGTTAAAGA TGCAGTCAAA AGATTCAGGA CTAATTGCAT
     1981 CAAGAACACA GAGAAAGACA TATTTCTCAA GATCAGAAGT ACTATTCCAG TATGGACGAT
     2041 TCAAGGCTTG CTTCATAAAC CAAGGCAAGT AATAGAGATT GGAGTCTCTA AAAAGGTAGT
     2101 TCCTACTGAA TCTAAGGCCA TGCATGGAGT CTAAGATTCA AATCGAGGAT CTAACAGAAC
     2161 TCGCCGTGAA GACTGGCGAA CAGTTCATAC AGAGTCTTTT ACGACTCAAT GACAAGAAGA
     2221 AAATCTTCGT CAACATGGTG GAGCACGACA CTCTGGTCTA CTCCAAAAAT GTCAAAGATA
     2281 CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGATAATT TCGGGAAACC
     2341 TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG
     2401 GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCATTCAA GATCTCTCTG
     2461 CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG
     2521 TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG
     2581 ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT
     2641 TGGAGAGGAC ACGctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
     2701 acaacaaaca acattacaat tacatttaca attaccatgt acccatacga tgttccagat
     2761 tacgctATCA CAAGTTTGTA CAAAAAAGCA GGCTGCATGA TGCCAAGCAA ACTCGCGCTA
     2821 ATCCAGGAAC TTCCCGACCG CATTCAAACG GCGGTGGAAG CAGCCATGGG AATGAGCTAC
     2881 CAAGACGCAC CGAACAACGT GCGCAGGGAC CTCGACAACC TGCACGCTTG CCTAAACAAG
     2941 GCAAAACTAA CGGTAAGTCG GATGGTAACA TCACTGCTGG AGAAACCCAG CGTGGTGGCA
     3001 TACCTAGAGG GAAAGGCCGA CCCAGCTTTC TTGTACAAAG TGGTGCCTAG GAGTATCGCT
     3061 ACAATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG
     3121 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC
     3181 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC
     3241 ACCCTCGTGA CCACCTTCGG CTACGGCCTG CAGTGCTTCG CCCGCTACCC CGACCACATG
     3301 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC
     3361 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC
     3421 CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG
     3481 CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG
     3541 AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACTGACC TAGGTGAGTC
     3601 TAGAGAGTTA ATTCTAGGTG AGTCTAGAGA GTTAATTAAG ACCCGGGACT Agtccctaga
     3661 gtcctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct
     3721 gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg
     3781 ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt
     3841 caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt
     3901 gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt
     3961 tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg
     4021 attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc
     4081 gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg
     4141 tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc
     4201 aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt
     4261 tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat
     4321 cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcag
     4381 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4441 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4501 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
     4561 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
     4621 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa
     4681 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     4741 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
     4801 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
     4861 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
     4921 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
     4981 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
     5041 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
     5101 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
     5161 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     5221 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     5281 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     5341 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
     5401 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     5461 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
     5521 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
     5581 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
     5641 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
     5701 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
     5761 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
     5821 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
     5881 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
     5941 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
     6001 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
     6061 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
     6121 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     6181 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     6241 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     6301 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     6361 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     6421 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     6481 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     6541 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     6601 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     6661 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     6721 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     6781 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     6841 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     6901 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg
     6961 caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc
     7021 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
     7081 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
     7141 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
     7201 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
     7261 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
     7321 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
     7381 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
     7441 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
     7501 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
     7561 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
     7621 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
     7681 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
     7741 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
     7801 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
     7861 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
     7921 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
     7981 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
     8041 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
     8101 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc
     8161 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
     8221 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
     8281 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
     8341 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
     8401 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
     8461 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
     8521 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     8581 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8641 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     8701 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     8761 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     8821 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8881 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8941 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9001 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9061 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9121 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9181 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9241 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9301 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9361 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9421 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9481 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9541 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9601 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9661 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
     9721 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
     9781 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
     9841 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
     9901 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
     9961 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
    10021 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
    10081 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
    10141 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
    10201 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
    10261 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
    10321 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
    10381 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
    10441 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
    10501 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
    10561 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
    10621 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
    10681 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
    10741 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
    10801 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
    10861 agctgccggt cggggagctg ttggctggct gg
//

