LOCUS       pDRBFC-B2-YC           10595 bp    DNA     linear       01-APR-2016
FEATURES             Location/Qualifiers
     misc_feature    3355..4081
                     /note="OCS terminator"
     misc_feature    4318..4342
                     /note="RB"
     misc_feature    5..25
                     /note="LB"
     CDS             complement(373..924)
                     /note="Bar"
                     /translation=""
     misc_feature    2229..2649
                     /note="35S promoter"
     CDS             2794..3012
                     /note="B2"
                     /translation="MPSKLALIQELPDRIQTAVEAAMGMSYQDAPNNVRRDLDNLHAC
                     LNKAKLTVSRMVTSLLEKPSVVAYLEGKA"
     CDS             2737..2763
                     /note="FLAG"
                     /translation="MDYKDDDDK"
     CDS             3094..3288
                     /note="YC"
                     /translation="GSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     CDS             2737..3288
                     /note="FLAG-B2-YC fusion protein"
                     /translation="MDYKDDDDKITSLYKKAGFMPSKLALIQELPDRIQTAVEAAMGM
                     SYQDAPNNVRRDLDNLHACLNKAKLTVSRMVTSLLEKPSVVAYLEGKADPAFLYKVVP
                     RPACKIPNDLKQKVMNHGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEK
                     RDHMVLLEFVTAAGITLGMDELYK"
     source          1..10595
                     /dnas_title=" pDRBFC-B2-YC vector"
ORIGIN      
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcgGATCT GATAATTTAT
      121 TTGAAAATTC ATAAGAAAAG CAAACGTTAC ATGAATTGAT GAAACAATAC AAAGACAGAT
      181 AAAGCCACGC ACATTTAGGA TATTGGCCGA GATTACTGAA TATTGAGTAA GATCACGGAA
      241 TTTCTGACAG GAGCATGTCT TCAATTCAGC CCAAATGGCA GTTGAAATAC TCAAACCGCC
      301 CCATATGCAG GAGCGGATCA TTCATTGTTT GTTTGGTTGC CTTTGCCAAC ATGGGAGTCC
      361 AAGATTCTGC AGTCAAATCT CGGTGACGGG CAGGACCGGA CGGGGCGGTA CCGGCAGGCT
      421 GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG
      481 CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG
      541 CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGGTG
      601 GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGGAGACGT ACACGGTCGA
      661 CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGG CCCGCGTAGG CGATGCCGGC
      721 GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC
      781 GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT
      841 GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC
      901 GGCCGGGCGT CGTTCTGGGC TCATCGATTC GATTTGGTGT ATCGAGATTG GTTATGAAAT
      961 TCAGATGCTA GTGTAATGTA TTGGTAATTT GGGAAGATAT AATAGGAAGC AAGGCTATTT
     1021 ATCCATTTCT GAAAAGGCGA AATGGCGTCA CCGCGAGCGT CACGCGCATT CCGTTCTTGC
     1081 TGTAAAGCGT TGTTTGGTAC ACTTTTGACT AGCGAGGCTT GGCGTGTCAG CGTATCTATT
     1141 CAAAAGTCGT TAATGGCTGC GGATCAAGAA AAAGTTGGAA TAGAAACAGA ATACCCGCGA
     1201 AATTCAGGCC CGGTTGCCAT GTCCTACACG CCGAAATAAA CGACCAAATT AGTAGAAAAA
     1261 TAAAAACTGA CTCGGATACT TACGTCACGT CTTGCGCACT GATTTGAAAA ATCTCAGaat
     1321 TCCAATCCCA CAAAAAtCTG AgCtTAaCAG caCAGTTGCT CCTCTCAGAG caGAATCGGG
     1381 TATTCAACAC CCTCATAtCA ACTACTACGT tGTGTATAAC GGtCCaCATG CCGGTATATA
     1441 CGATGACTGG GGTTGTACAA AGGCgGCAAC AAACGGcGTT CCCGGAGTTG CaCAcAAGAA
     1501 aTTTGCCACT ATTACAGAGG CAAGAGCAGC AGCTGACGCG TAcACAACAA GTCAGCAAAC
     1561 AGAcAGGTTG AACTTCATCC CCAAAGGAGA AGCTCAACTC AAGCCCAAGA GCTTTGCtAA
     1621 GGCCCTAACA AGCCCACCAA AGCAAAAAGC CCACTGgCTC ACGCTAGGAA CCAAAAGGCC
     1681 CAGCAGTGAT CCAGCCCCAA AAGAGAtCTC CTTTGCCCCG GAGATTACAA TGGACGATTT
     1741 CCTCTATCTT TACGATCTAG GAAGGAAGTT CGAAGGTGAA GGTGACGACA CTATGTTCAC
     1801 CACTGATAAT GAGAAGGTTA GCCTCTTCAA TTTCAGAAAG AATGCTGACC CACAGATGGT
     1861 TAGAGAGGCC TACGCAGCAG GTCTCATCAA GACGATCTAC CCGAGTAACA ATCTCCAGGA
     1921 GATCAAATAC CTTCCCAAGA AGGTTAAAGA TGCAGTCAAA AGATTCAGGA CTAATTGCAT
     1981 CAAGAACACA GAGAAAGACA TATTTCTCAA GATCAGAAGT ACTATTCCAG TATGGACGAT
     2041 TCAAGGCTTG CTTCATAAAC CAAGGCAAGT AATAGAGATT GGAGTCTCTA AAAAGGTAGT
     2101 TCCTACTGAA TCTAAGGCCA TGCATGGAGT CTAAGATTCA AATCGAGGAT CTAACAGAAC
     2161 TCGCCGTGAA GACTGGCGAA CAGTTCATAC AGAGTCTTTT ACGACTCAAT GACAAGAAGA
     2221 AAATCTTCGT CAACATGGTG GAGCACGACA CTCTGGTCTA CTCCAAAAAT GTCAAAGATA
     2281 CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGATAATT TCGGGAAACC
     2341 TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG
     2401 GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCATTCAA GATCTCTCTG
     2461 CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG
     2521 TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG
     2581 ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT
     2641 TGGAGAGGAC ACGCTCGAGT ATAAGAGCTC TATTTTTACA ACAATTACCA ACAACAACAA
     2701 ACAACAAACA ACATTACAAT TACATTTACA ATTACCATGG ACTACAAAGA CGATGACGAC
     2761 AAAATCACAA GTTTGTACAA AAAAGCAGGC TTCATGCCAA GCAAACTCGC GCTAATCCAG
     2821 GAACTTCCCG ACCGCATTCA AACGGCGGTG GAAGCAGCCA TGGGAATGAG CTACCAAGAC
     2881 GCACCGAACA ACGTGCGCAG GGACCTCGAC AACCTGCACG CTTGCCTAAA CAAGGCAAAA
     2941 CTAACGGTAA GTCGGATGGT AACATCACTG CTGGAGAAAC CCAGCGTGGT GGCATACCTA
     3001 GAGGGAAAGG CCGACCCAGC TTTCTTGTAC AAAGTGGTGC CTAGGCCTGC TTGTAAGATC
     3061 CCTAACGACC TCAAGCAGAA AGTCATGAAC CACGGCAGCG TGCAGCTCGC CGACCACTAC
     3121 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
     3181 TACCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
     3241 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTG ACCTAGGTGA
     3301 GTCTAGAGAG TTAATTCTAG GTGAGTCTAG AGAGTTAATT AAGACCCGGG ACTAgtccct
     3361 agagtcctgc tttaatgaga tatgcgagac gcctatgatc gcatgatatt tgctttcaat
     3421 tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta ccgccggttt
     3481 cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat aatattctcc
     3541 gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg aaaacaatat
     3601 attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa caaacaattg
     3661 cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa acctaaaaga
     3721 ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta tctacgacac
     3781 accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc cggcgcgcat
     3841 gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt acgggcacca
     3901 ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga attatgcagc
     3961 atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac agtgacgaca
     4021 aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca gcaggacctg
     4081 caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4141 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4201 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag
     4261 cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
     4321 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt
     4381 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     4441 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
     4501 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
     4561 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
     4621 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
     4681 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
     4741 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
     4801 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
     4861 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     4921 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     4981 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     5041 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
     5101 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     5161 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     5221 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     5281 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     5341 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     5401 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     5461 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     5521 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     5581 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     5641 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     5701 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     5761 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     5821 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     5881 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     5941 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     6001 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     6061 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     6121 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     6181 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     6241 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     6301 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     6361 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     6421 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     6481 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     6541 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     6601 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     6661 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg
     6721 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     6781 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     6841 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     6901 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     6961 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     7021 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     7081 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     7141 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     7201 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     7261 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     7321 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     7381 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     7441 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     7501 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     7561 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     7621 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     7681 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     7741 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     7801 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     7861 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     7921 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     7981 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     8041 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     8101 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     8161 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     8221 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     8281 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     8341 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     8401 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8461 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     8521 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8581 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8641 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8701 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8761 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8821 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8881 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8941 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9001 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9061 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9121 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9181 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9241 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9301 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9361 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
     9421 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
     9481 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
     9541 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
     9601 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
     9661 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
     9721 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
     9781 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
     9841 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
     9901 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
     9961 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
    10021 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
    10081 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
    10141 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
    10201 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    10261 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    10321 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
    10381 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
    10441 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
    10501 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
    10561 ccgagctgcc ggtcggggag ctgttggctg gctgg
//

