LOCUS pDRBFC-NS1-YC 10586 bp DNA linear 01-APR-2016 FEATURES Location/Qualifiers misc_feature 3346..4072 /note="OCS terminator" misc_feature 4309..4333 /note="RB" misc_feature 5..25 /note="LB" CDS complement(373..924) /note="Bar" /translation="" misc_feature 2229..2649 /note="35S promoter" CDS 2737..2763 /note="FLAG" /translation="MDYKDDDDK" CDS 2794..3003 /note="NS1" /translation="MDPNTVSSFQVDCFLWHVRKRVADQELGDAPFLDRLRRDQKSLR GRGSTLGLDIETATRAGKQIVERILK" CDS 3085..3279 /note="YC" /translation="GSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK" CDS 2737..3279 /note="FLAG-NS1-YC fusion protein" /translation="MDYKDDDDKITSLYKKAGFMDPNTVSSFQVDCFLWHVRKRVADQ ELGDAPFLDRLRRDQKSLRGRGSTLGLDIETATRAGKQIVERILKDPAFLYKVVPRPA CKIPNDLKQKVMNHGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK" source 1..10586 /dnas_title="pDRBFC-NS1-YC vector" ORIGIN 1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg 61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcgGATCT GATAATTTAT 121 TTGAAAATTC ATAAGAAAAG CAAACGTTAC ATGAATTGAT GAAACAATAC AAAGACAGAT 181 AAAGCCACGC ACATTTAGGA TATTGGCCGA GATTACTGAA TATTGAGTAA GATCACGGAA 241 TTTCTGACAG GAGCATGTCT TCAATTCAGC CCAAATGGCA GTTGAAATAC TCAAACCGCC 301 CCATATGCAG GAGCGGATCA TTCATTGTTT GTTTGGTTGC CTTTGCCAAC ATGGGAGTCC 361 AAGATTCTGC AGTCAAATCT CGGTGACGGG CAGGACCGGA CGGGGCGGTA CCGGCAGGCT 421 GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG 481 CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG 541 CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGGTG 601 GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGGAGACGT ACACGGTCGA 661 CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGG CCCGCGTAGG CGATGCCGGC 721 GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC 781 GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT 841 GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC 901 GGCCGGGCGT CGTTCTGGGC TCATCGATTC GATTTGGTGT ATCGAGATTG GTTATGAAAT 961 TCAGATGCTA GTGTAATGTA TTGGTAATTT GGGAAGATAT AATAGGAAGC AAGGCTATTT 1021 ATCCATTTCT GAAAAGGCGA AATGGCGTCA CCGCGAGCGT CACGCGCATT CCGTTCTTGC 1081 TGTAAAGCGT TGTTTGGTAC ACTTTTGACT AGCGAGGCTT GGCGTGTCAG CGTATCTATT 1141 CAAAAGTCGT TAATGGCTGC GGATCAAGAA AAAGTTGGAA TAGAAACAGA ATACCCGCGA 1201 AATTCAGGCC CGGTTGCCAT GTCCTACACG CCGAAATAAA CGACCAAATT AGTAGAAAAA 1261 TAAAAACTGA CTCGGATACT TACGTCACGT CTTGCGCACT GATTTGAAAA ATCTCAGaat 1321 TCCAATCCCA CAAAAAtCTG AgCtTAaCAG caCAGTTGCT CCTCTCAGAG caGAATCGGG 1381 TATTCAACAC CCTCATAtCA ACTACTACGT tGTGTATAAC GGtCCaCATG CCGGTATATA 1441 CGATGACTGG GGTTGTACAA AGGCgGCAAC AAACGGcGTT CCCGGAGTTG CaCAcAAGAA 1501 aTTTGCCACT ATTACAGAGG CAAGAGCAGC AGCTGACGCG TAcACAACAA GTCAGCAAAC 1561 AGAcAGGTTG AACTTCATCC CCAAAGGAGA AGCTCAACTC AAGCCCAAGA GCTTTGCtAA 1621 GGCCCTAACA AGCCCACCAA AGCAAAAAGC CCACTGgCTC ACGCTAGGAA CCAAAAGGCC 1681 CAGCAGTGAT CCAGCCCCAA AAGAGAtCTC CTTTGCCCCG GAGATTACAA TGGACGATTT 1741 CCTCTATCTT TACGATCTAG GAAGGAAGTT CGAAGGTGAA GGTGACGACA CTATGTTCAC 1801 CACTGATAAT GAGAAGGTTA GCCTCTTCAA TTTCAGAAAG AATGCTGACC CACAGATGGT 1861 TAGAGAGGCC TACGCAGCAG GTCTCATCAA GACGATCTAC CCGAGTAACA ATCTCCAGGA 1921 GATCAAATAC CTTCCCAAGA AGGTTAAAGA TGCAGTCAAA AGATTCAGGA CTAATTGCAT 1981 CAAGAACACA GAGAAAGACA TATTTCTCAA GATCAGAAGT ACTATTCCAG TATGGACGAT 2041 TCAAGGCTTG CTTCATAAAC CAAGGCAAGT AATAGAGATT GGAGTCTCTA AAAAGGTAGT 2101 TCCTACTGAA TCTAAGGCCA TGCATGGAGT CTAAGATTCA AATCGAGGAT CTAACAGAAC 2161 TCGCCGTGAA GACTGGCGAA CAGTTCATAC AGAGTCTTTT ACGACTCAAT GACAAGAAGA 2221 AAATCTTCGT CAACATGGTG GAGCACGACA CTCTGGTCTA CTCCAAAAAT GTCAAAGATA 2281 CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGATAATT TCGGGAAACC 2341 TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG 2401 GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCATTCAA GATCTCTCTG 2461 CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG 2521 TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG 2581 ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT 2641 TGGAGAGGAC ACGCTCGAGT ATAAGAGCTC TATTTTTACA ACAATTACCA ACAACAACAA 2701 ACAACAAACA ACATTACAAT TACATTTACA ATTACCATGG ACTACAAAGA CGATGACGAC 2761 AAAATCACAA GTTTGTACAA AAAAGCAGGC TTCATGGATC CAAACACTGT GTCAAGCTTT 2821 CAGGTAGATT GCTTTCTTTG GCATGTCCGC AAACGAGTTG CAGACCAAGA ACTAGGTGAT 2881 GCCCCATTCC TTGATCGGCT TCGCCGAGAT CAGAAATCCC TAAGAGGAAG GGGCAGCACC 2941 CTCGGTCTGG ACATCGAGAC AGCCACACGT GCTGGAAAGC AGATAGTGGA GCGGATTCTG 3001 AAAGACCCAG CTTTCTTGTA CAAAGTGGTG CCTAGGCCTG CTTGTAAGAT CCCTAACGAC 3061 CTCAAGCAGA AAGTCATGAA CCACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC 3121 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC 3181 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC 3241 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT GACCTAGGTG AGTCTAGAGA 3301 GTTAATTCTA GGTGAGTCTA GAGAGTTAAT TAAGACCCGG GACTAgtccc tagagtcctg 3361 ctttaatgag atatgcgaga cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg 3421 cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt tcggttcatt 3481 ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc cgttcaattt 3541 actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata tattgtgctg 3601 aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt gcgttttatt 3661 attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag actgattaca 3721 taaatcttat tcaaatttca aaagtgcccc aggggctagt atctacgaca caccgagcgg 3781 cgaactaata acgctcactg aagggaactc cggttccccg ccggcgcgca tgggtgagat 3841 tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc attcaacccg 3901 gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag catttttttg 3961 gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac aaatcgttgg 4021 gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggacct gcaggcatgc 4081 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 4141 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 4201 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag 4261 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata 4321 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg 4381 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg 4441 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca 4501 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc 4561 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact 4621 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc 4681 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg 4741 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag 4801 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac 4861 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc 4921 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg 4981 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc 5041 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc 5101 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa 5161 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc 5221 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc 5281 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc 5341 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg 5401 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc 5461 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa 5521 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg 5581 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt 5641 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc 5701 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag 5761 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg 5821 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg 5881 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat 5941 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa 6001 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat 6061 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat 6121 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac 6181 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga 6241 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac 6301 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg 6361 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag 6421 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc 6481 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa 6541 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg 6601 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg 6661 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac 6721 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc 6781 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc 6841 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc 6901 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg 6961 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg 7021 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg 7081 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat 7141 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg 7201 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg 7261 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca 7321 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg 7381 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc 7441 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga 7501 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag 7561 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga 7621 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga 7681 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg 7741 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag 7801 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc 7861 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt 7921 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt 7981 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca 8041 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg 8101 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca 8161 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag 8221 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 8281 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 8341 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga 8401 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 8461 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt 8521 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 8581 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 8641 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 8701 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 8761 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 8821 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 8881 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 8941 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 9001 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 9061 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 9121 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 9181 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 9241 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 9301 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt 9361 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct 9421 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga 9481 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat 9541 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt 9601 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg 9661 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat 9721 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg 9781 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag 9841 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca 9901 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt 9961 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc 10021 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag 10081 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg 10141 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat 10201 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa 10261 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt 10321 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta 10381 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc 10441 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg 10501 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc 10561 cggtcgggga gctgttggct ggctgg //