LOCUS       pDRBFC-NS1-YC           10586 bp    DNA     linear       01-APR-2016
FEATURES             Location/Qualifiers
     misc_feature    3346..4072
                     /note="OCS terminator"
     misc_feature    4309..4333
                     /note="RB"
     misc_feature    5..25
                     /note="LB"
     CDS             complement(373..924)
                     /note="Bar"
                     /translation=""
     misc_feature    2229..2649
                     /note="35S promoter"
     CDS             2737..2763
                     /note="FLAG"
                     /translation="MDYKDDDDK"
     CDS             2794..3003
                     /note="NS1"
                     /translation="MDPNTVSSFQVDCFLWHVRKRVADQELGDAPFLDRLRRDQKSLR
                     GRGSTLGLDIETATRAGKQIVERILK"
     CDS             3085..3279
                     /note="YC"
                     /translation="GSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     CDS             2737..3279
                     /note="FLAG-NS1-YC fusion protein"
                     /translation="MDYKDDDDKITSLYKKAGFMDPNTVSSFQVDCFLWHVRKRVADQ
                     ELGDAPFLDRLRRDQKSLRGRGSTLGLDIETATRAGKQIVERILKDPAFLYKVVPRPA
                     CKIPNDLKQKVMNHGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     source          1..10586
                     /dnas_title="pDRBFC-NS1-YC vector"
ORIGIN      
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcgGATCT GATAATTTAT
      121 TTGAAAATTC ATAAGAAAAG CAAACGTTAC ATGAATTGAT GAAACAATAC AAAGACAGAT
      181 AAAGCCACGC ACATTTAGGA TATTGGCCGA GATTACTGAA TATTGAGTAA GATCACGGAA
      241 TTTCTGACAG GAGCATGTCT TCAATTCAGC CCAAATGGCA GTTGAAATAC TCAAACCGCC
      301 CCATATGCAG GAGCGGATCA TTCATTGTTT GTTTGGTTGC CTTTGCCAAC ATGGGAGTCC
      361 AAGATTCTGC AGTCAAATCT CGGTGACGGG CAGGACCGGA CGGGGCGGTA CCGGCAGGCT
      421 GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG
      481 CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG
      541 CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGGTG
      601 GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGGAGACGT ACACGGTCGA
      661 CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGG CCCGCGTAGG CGATGCCGGC
      721 GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC
      781 GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT
      841 GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC
      901 GGCCGGGCGT CGTTCTGGGC TCATCGATTC GATTTGGTGT ATCGAGATTG GTTATGAAAT
      961 TCAGATGCTA GTGTAATGTA TTGGTAATTT GGGAAGATAT AATAGGAAGC AAGGCTATTT
     1021 ATCCATTTCT GAAAAGGCGA AATGGCGTCA CCGCGAGCGT CACGCGCATT CCGTTCTTGC
     1081 TGTAAAGCGT TGTTTGGTAC ACTTTTGACT AGCGAGGCTT GGCGTGTCAG CGTATCTATT
     1141 CAAAAGTCGT TAATGGCTGC GGATCAAGAA AAAGTTGGAA TAGAAACAGA ATACCCGCGA
     1201 AATTCAGGCC CGGTTGCCAT GTCCTACACG CCGAAATAAA CGACCAAATT AGTAGAAAAA
     1261 TAAAAACTGA CTCGGATACT TACGTCACGT CTTGCGCACT GATTTGAAAA ATCTCAGaat
     1321 TCCAATCCCA CAAAAAtCTG AgCtTAaCAG caCAGTTGCT CCTCTCAGAG caGAATCGGG
     1381 TATTCAACAC CCTCATAtCA ACTACTACGT tGTGTATAAC GGtCCaCATG CCGGTATATA
     1441 CGATGACTGG GGTTGTACAA AGGCgGCAAC AAACGGcGTT CCCGGAGTTG CaCAcAAGAA
     1501 aTTTGCCACT ATTACAGAGG CAAGAGCAGC AGCTGACGCG TAcACAACAA GTCAGCAAAC
     1561 AGAcAGGTTG AACTTCATCC CCAAAGGAGA AGCTCAACTC AAGCCCAAGA GCTTTGCtAA
     1621 GGCCCTAACA AGCCCACCAA AGCAAAAAGC CCACTGgCTC ACGCTAGGAA CCAAAAGGCC
     1681 CAGCAGTGAT CCAGCCCCAA AAGAGAtCTC CTTTGCCCCG GAGATTACAA TGGACGATTT
     1741 CCTCTATCTT TACGATCTAG GAAGGAAGTT CGAAGGTGAA GGTGACGACA CTATGTTCAC
     1801 CACTGATAAT GAGAAGGTTA GCCTCTTCAA TTTCAGAAAG AATGCTGACC CACAGATGGT
     1861 TAGAGAGGCC TACGCAGCAG GTCTCATCAA GACGATCTAC CCGAGTAACA ATCTCCAGGA
     1921 GATCAAATAC CTTCCCAAGA AGGTTAAAGA TGCAGTCAAA AGATTCAGGA CTAATTGCAT
     1981 CAAGAACACA GAGAAAGACA TATTTCTCAA GATCAGAAGT ACTATTCCAG TATGGACGAT
     2041 TCAAGGCTTG CTTCATAAAC CAAGGCAAGT AATAGAGATT GGAGTCTCTA AAAAGGTAGT
     2101 TCCTACTGAA TCTAAGGCCA TGCATGGAGT CTAAGATTCA AATCGAGGAT CTAACAGAAC
     2161 TCGCCGTGAA GACTGGCGAA CAGTTCATAC AGAGTCTTTT ACGACTCAAT GACAAGAAGA
     2221 AAATCTTCGT CAACATGGTG GAGCACGACA CTCTGGTCTA CTCCAAAAAT GTCAAAGATA
     2281 CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGATAATT TCGGGAAACC
     2341 TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG
     2401 GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCATTCAA GATCTCTCTG
     2461 CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG
     2521 TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG
     2581 ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT
     2641 TGGAGAGGAC ACGCTCGAGT ATAAGAGCTC TATTTTTACA ACAATTACCA ACAACAACAA
     2701 ACAACAAACA ACATTACAAT TACATTTACA ATTACCATGG ACTACAAAGA CGATGACGAC
     2761 AAAATCACAA GTTTGTACAA AAAAGCAGGC TTCATGGATC CAAACACTGT GTCAAGCTTT
     2821 CAGGTAGATT GCTTTCTTTG GCATGTCCGC AAACGAGTTG CAGACCAAGA ACTAGGTGAT
     2881 GCCCCATTCC TTGATCGGCT TCGCCGAGAT CAGAAATCCC TAAGAGGAAG GGGCAGCACC
     2941 CTCGGTCTGG ACATCGAGAC AGCCACACGT GCTGGAAAGC AGATAGTGGA GCGGATTCTG
     3001 AAAGACCCAG CTTTCTTGTA CAAAGTGGTG CCTAGGCCTG CTTGTAAGAT CCCTAACGAC
     3061 CTCAAGCAGA AAGTCATGAA CCACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
     3121 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC
     3181 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
     3241 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT GACCTAGGTG AGTCTAGAGA
     3301 GTTAATTCTA GGTGAGTCTA GAGAGTTAAT TAAGACCCGG GACTAgtccc tagagtcctg
     3361 ctttaatgag atatgcgaga cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg
     3421 cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt tcggttcatt
     3481 ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc cgttcaattt
     3541 actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata tattgtgctg
     3601 aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt gcgttttatt
     3661 attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag actgattaca
     3721 taaatcttat tcaaatttca aaagtgcccc aggggctagt atctacgaca caccgagcgg
     3781 cgaactaata acgctcactg aagggaactc cggttccccg ccggcgcgca tgggtgagat
     3841 tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc attcaacccg
     3901 gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag catttttttg
     3961 gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac aaatcgttgg
     4021 gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggacct gcaggcatgc
     4081 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4141 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4201 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag
     4261 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     4321 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
     4381 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     4441 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     4501 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     4561 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     4621 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     4681 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     4741 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     4801 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     4861 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     4921 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     4981 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     5041 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     5101 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     5161 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     5221 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     5281 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     5341 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     5401 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
     5461 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     5521 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     5581 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     5641 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     5701 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     5761 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     5821 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     5881 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     5941 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     6001 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     6061 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     6121 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     6181 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     6241 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     6301 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     6361 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     6421 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     6481 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     6541 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     6601 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg
     6661 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac
     6721 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
     6781 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     6841 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     6901 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     6961 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     7021 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     7081 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     7141 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     7201 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     7261 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     7321 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     7381 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     7441 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     7501 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     7561 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     7621 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     7681 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     7741 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     7801 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     7861 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     7921 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     7981 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     8041 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
     8101 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
     8161 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
     8221 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8281 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     8341 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     8401 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     8461 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     8521 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8581 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8641 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8701 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8761 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8821 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8881 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8941 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9001 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9061 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9121 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9181 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9241 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9301 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt
     9361 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
     9421 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
     9481 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
     9541 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
     9601 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
     9661 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
     9721 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
     9781 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
     9841 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
     9901 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
     9961 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
    10021 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
    10081 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
    10141 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
    10201 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
    10261 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
    10321 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta
    10381 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
    10441 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
    10501 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
    10561 cggtcgggga gctgttggct ggctgg
//

