LOCUS       Exported                7800 bp ds-DNA     circular SYN 17-MAY-2016
DEFINITION  Thorold W. Theunissen.
ACCESSION   .
VERSION     .
KEYWORDS    74910_Genbank
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 17, 2016 from SnapGene 3.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..1724
                     /note="CAG promoter"
     misc_feature    1748..1772
                     /note="attB1"
     misc_feature    1778..2029
                     /note="zNanog HD"
                     /note="Homeodomain of zebrafish Nanog"
     misc_feature    2031..2055
                     /note="attB2"
     misc_feature    2256..2478
                     /note="polyA"
     misc_feature    2557
                     /note="loxP"
     misc_feature    complement(2593..2798)
                     /note="Piggy 3'"
     misc_feature    complement(3586..4446)
                     /note="AmpR"
     misc_feature    complement(4447..5387)
                     /note="ColE1 ori"
     misc_feature    complement(5665..5974)
                     /note="Piggy 5'"
     misc_feature    complement(5975..6180)
                     /note="PGK polyA"
     misc_feature    complement(6186..7211)
                     /note="Hygro"
     misc_feature    complement(7232..7738)
                     /note="PGK"
     misc_feature    7752
                     /note="loxP"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      421 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      481 atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg
      721 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      781 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct
      841 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt
      901 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg
      961 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt
     1021 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1081 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct
     1141 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg
     1201 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     1261 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc
     1321 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1381 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     1441 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1501 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgac ggtatcgata
     1741 agctatcaca agtttgtaca aaaaagcagg cttcaccatg cggcctccga ccctgccccg
     1801 caagacccgg gctgcttttt ctgaagaaca gatgaatgct ttggtcaatc ggtttaatgt
     1861 ccagagatac ctcacgcctg cagagatgaa aacgcttgct ggagcaacag gattaacata
     1921 caagcaggta aaaacatggt ttcagaaccg caggatgaaa ctcaagaggc atcagagaga
     1981 cagcagttgg atgactgaga gatatgttgt caatgctgta cccaactaag acccagcttt
     2041 cttgtacaaa gtggtgatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     2101 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2161 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2221 tcgggaaacc tgtcgtgcca gcggatcagc ctcgactgtg ccttctagtt gccagccatc
     2281 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     2341 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     2401 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     2461 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgagatccac
     2521 tagttctagc ctcgaggcta gagcggccgc gtcgagataa cttcgtataa tgtatgctat
     2581 acgaagttat gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     2641 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     2701 ataaaactta atatctattc aaattaataa ataaacgtca attttacgca tgattatctt
     2761 taacgtacgt cacaatatga ttatctttct agggttaaag agctccaatt cgccctatag
     2821 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     2881 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     2941 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     3001 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3061 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3121 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3181 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     3241 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3301 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3361 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3421 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     3481 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3541 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3601 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3661 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3721 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3781 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3841 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3901 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3961 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4021 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4081 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4141 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4201 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4261 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4321 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4381 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4441 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4501 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4561 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4621 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4681 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4741 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4801 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4861 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4921 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4981 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5041 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5101 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5161 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5221 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5281 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5341 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5401 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     5461 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     5521 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     5581 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     5641 taaagggaac aaaagctggg taccttaacc ctagaaagat agtctgcgta aaattgacgc
     5701 atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg ctgtgcattt
     5761 aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt
     5821 cactgatttt gaactataac gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt
     5881 gacttttaag atttaactca tacgataatt atattgttat ttcatgttct acttacgtga
     5941 taacttatta tatatatatt ttcttgttat agatatccaa ctatgaaaca ttatcataaa
     6001 gcaatagtaa agagccttca gtaaagagca ggcatttatc taatcccacc ccacccccac
     6061 ccccgtagct ccaatccttc cattcaaaat gtaggtactc tgttctcacc cttcttaaca
     6121 aagtatgaca ggaaaaaact tccatgtgga catctttatt gtttaataga tcatcaattt
     6181 ctcgactatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     6241 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     6301 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     6361 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     6421 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     6481 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     6541 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     6601 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     6661 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     6721 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     6781 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     6841 gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca
     6901 ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg
     6961 ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
     7021 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
     7081 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
     7141 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
     7201 ggctttttca tggtggcggc tggatcggtc gaaaggcccg gagatgagga agaggagaac
     7261 agcgcggcag acgtgcgctt ttgaagcgtg cgagaatgcc gggcctccgg aggaccttcg
     7321 ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag
     7381 cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac
     7441 ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc
     7501 catttgtcac gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg
     7561 aggagtagaa ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt
     7621 ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc cagcggggct
     7681 gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg gtagaattca
     7741 tcgatgatat cataacttcg tataatgtat gctatacgaa gttatgcggc cctcgaggtc
//
