LOCUS       pJJR82        10481 bp ds-DNA     circular     16-MAR-2016
DEFINITION  Cloning vector pUC19, complete sequence.
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19 other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     pDD282 backbone, GFP-C1 replaced with worm codon-optimized eGFP
COMMENT     To prepare vector for cloning: 
COMMENT     -Digest with ClaI and SpeI to fuse 5' homology arm directly to the
            tag, or 
COMMENT     -Digest with AvrII and SpeI to include a flexible linker (for
            C-terminal tags).
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    404..1010
                     /label=This sequence will be replaced by the homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(2772..2912,2960..3793)
                     /label=SQT-1
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6102..7127
                     /note="hygromycinR"
                     /label=hygR
                     /ApEinfo_fwdcolor=#006666
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(1038..1340,1394..1468,1520..1639,1691..1906)
                     /label=eGFP cai 1.0
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1988..2017
                     /label=myc
                     /ApEinfo_fwdcolor=#6666ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        complement(8662..9214)
                     /note="RNAII transcript (complementary strand)"
                     /label=RNAII transcript (complementary strand)
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        9104..9211
                     /note="RNAI transcript"
                     /label=RNAI transcript
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(join(4166..4407,4459..4829,4881..5296))
                     /label=Cre
                     /ApEinfo_fwdcolor=#6699cc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8662..9250)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /label=pMB1 origin of replication (counter-clockwise) (RN
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      9068..9073
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /label=RNAI promoter (clockwise); TTGAAG
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      complement(9245..9250)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /label=RNAII promoter (counter-clockwise); TTGAGA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     378..395
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1934..1944
                     /label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      9090..9095
                     /note="RNAI promoter (clockwise); GCTACA"
                     /label=RNAI promoter (clockwise); GCTACA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1945..1978
                     /label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      complement(9224..9229)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /label=RNAII promoter (counter-clockwise); CGTAAT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9424..10083)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1985..1987
                     /label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 3023..3023
                     /label=e1350
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9421..10281)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2021..2401
                     /label=let-858 Terminator
                     /ApEinfo_fwdcolor=#cc0000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1979..1984
                     /label=intron(1)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(8256..8276)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     5'UTR           join(2686..2713,2770..2771)
                     /label=sqt-1 5'UTR
                     /ApEinfo_fwdcolor=#ccffcc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1907..1927
                     /label=TEV site
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    661..966
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8644..9326)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           3794..3830
                     /label=sqt-1 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(5297..5696)
                     /label=hsp16-41 promoter
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7611..7637
                     /label=Flag
                     /ApEinfo_fwdcolor=#ff6699
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2402..2771
                     /label=Psqt1
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             754..963
                     /label=ccdB(1)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7584..7610
                     /label=Flag(1)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7545..7556
                     /label=spacer(1)
                     /ApEinfo_label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7557..7583
                     /label=Flag(2)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#cc3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           complement(3831..4162)
                     /label=tbb-2 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#993333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          7539..7544
                     /label=intron(2)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           7153..7504
                     /label=unc-54 3'UTR
                     /ApEinfo_fwdcolor=#990000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7505..7538
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        5697..6059
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;
                     /ApEinfo_revcolor=pink; /ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
                     /label=Prps-0
                     /ApEinfo_fwdcolor=#339933
                     /ApEinfo_revcolor=#339933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8245..8251
                     /label=This sequence will be deleted if ClaI is used
                     /ApEinfo_fwdcolor=#ffcc99
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7638..8244
                     /label=This sequence will be replaced by the homology
                     arm(1)
                     /ApEinfo_label=This sequence will be replaced by the
                     homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(7682..7987)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB(2)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(7685..7894)
                     /label=ccdB(3)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_structure  1011..1037
                     /label=Flexible Linker (will be deleted if ClaI is used)
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtTA CCCGAAGTAT
      421 GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT
      481 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTTC GGTAAGCACA ACCATGCAGA
      541 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG
      601 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCCGA CGAGAACAGG GGCTGGTGAA
      661 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA
      721 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT
      781 CTGCTGTCAG ATAAAGTCCC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC
      841 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG
      901 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA
      961 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGAcctag GGAGCATCGG
     1021 GAGCCTCAGG AGCATCGATG TCCAAGGGAG AGGAGCTCTT CACCGGAGTC GTCCCAATCC
     1081 TCGTCGAGCT CGACGGAGAC GTCAACGGAC ACAAGTTCTC CGTCTCAGGA GAGGGAGAGG
     1141 GAGACGCCAC CTACGGAAAG CTCACCCTCA AGTTCATCTG CACCACCGGA AAGCTCCCAG
     1201 TCCCATGGCC AACCCTCGTC ACCACCTTCA CTTACGGAGT CCAATGCTTC TCCCGTTACC
     1261 CAGACCACAT GAAGCGTCAC GACTTCTTCA AGTCCGCCAT GCCAGAGGGA TACGTCCAAG
     1321 AGCGTACCAT CTTCTTCAAG gtaagtttaa acattaatta atactaacta accctgatta
     1381 tttaaatttt cagGACGACG GAAACTACAA GACCCGTGCC GAGGTCAAGT TCGAGGGAGA
     1441 CACCCTCGTC AACCGTATCG AGCTCAAGgt aagtttaaac agttcggtac taactaacca
     1501 tacatattta aattttcagG GAATCGACTT CAAGGAGGAC GGAAACATCC TCGGACACAA
     1561 GCTCGAATAC AACTACAACT CCCACAACGT CTACATCATG GCCGACAAGC AAAAGAACGG
     1621 AATCAAGGTC AACTTCAAGg taagtttaaa catgatttta ctaactaact aatctgattt
     1681 aaattttcag ATCCGTCACA ACATCGAGGA CGGATCTGTC CAACTCGCCG ACCACTACCA
     1741 ACAAAACACC CCAATCGGAG ACGGACCAGT CCTCCTCCCA GACAACCACT ACCTCTCCAC
     1801 CCAATCCGCC CTCTCCAAGG ACCCAAACGA GAAGCGTGAC CACATGGTCC TCAAGGAGTT
     1861 CGTCACCGCT GCCGGAATCA CCCACGGAAT GGACGAGCTC TACAAGGAGA ATCTGTACTT
     1921 TCAATCCGGA AAGgtaagtt taaaATAACT TCGTATAGCA TACATTATAC GAAGTTATtt
     1981 tcagGGAGAG CAAAAGCTTA TCTCCGAGGA GGACCTCTAA GGATGATCGA CGCCaACGTC
     2041 GTTGAATTTT CAAATTTTAA ATACTGAATA TTTGTTTTTT TTCCTATTAT TTATTTATTC
     2101 TCTTTGTGTT TTTTTTCTTG CTTTCTAAAA AATTAATTCA ATCCAAATCT AAacattttt
     2161 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag
     2221 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa
     2281 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc
     2341 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaacta
     2401 gctgccctcg attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt
     2461 cttctctggt tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca
     2521 cattcctcac ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc
     2581 tgcgtctcgc acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact
     2641 tgcggaaaga tttcctaata aaaggagacg aatccggtag gaaatcgaca agtctctgct
     2701 tctcgaagac taggtgagtt acatttcttt ggccgaatta ttttgaaatc ctaaatttga
     2761 taatttcaga gATGTCTGTA AAACTTGCGT GTTATGTGAC GGCTTCGGTC ACTGTCGCCA
     2821 CTCTTATGGT ATGCTTCATG ACCATGTCAA CCATCTACTC GGAGGTTGAT GGATTCAGAG
     2881 AGAAGCTTGA TACCGAGATG AATGTGTTCA GAgtgagtta aattatattt tgaattttaa
     2941 ataattttta attttctagC AATCTACCAA TGGATTGTGG AAGGACATAG TTGTCATCGG
     3001 AAGATCTAGC AAGCGTGTCC GTTGTCAATA TGAAGAGACC AACGCTACCC CAACTCCACA
     3061 TGCTGATGGA TCCCCATCTG CTCCACCAGG TCAACCACCA GCAGTTCCAC CAGTCTTCAA
     3121 CCAGCCAAAG ACTCCAAATG GAGCCAATGG AAATGGACCA ACCTGCAACT GCAATGCTGA
     3181 TAACAAGTGC CCAGCTGGAC CATCCGGACC AAAGGGAGTT CCAGGAGTTC CAGGACTCGA
     3241 CGGAGTTCCA GGACTTGACG GTGTTCCAGG AGTTGGAGCT GATGATATCG CTCCACAACG
     3301 CGAGTCTGTC GGATGCTTCA CTTGCCCACA AGGACCAGTT GGACCACCAG GAGCTCTTGG
     3361 AAGACCAGGA CCACGTGGAC TTCCAGGACC AAGAGGACAA AATGGAAACC CAGGAAGAGA
     3421 TGGACAACCA GGACATCCAG GAGAGCAAGG ATCATCCGGT CAAATCGGAA AGATCGGAGA
     3481 GCCAGGACCA CCAGGAGAGA AGGGACGCGA CGCCGAGCAT CCAATCGGAA GACCAGGACC
     3541 AAAGGGACCA AGAGGAGATC AAGGACCAAC AGGACCAGCT GGACAGAACG GTCTTCACGG
     3601 ACCACCAGGA GAGCCAGGAA CCGTTGGACC AGAAGGACCA TCTGGAAAGC AAGGACGTCA
     3661 AGGACCAGAC GGAACCCAGG GAGAGACTGG ACCAGACGGA AGACCAGGAA AGGATGCCGA
     3721 GTACTGCCAG TGCCCAGACA AGTCTCCACC ATCAGAGGCT GTCAACGCCA ACCGTGGATA
     3781 CAGAAATATC TAAattgttg gtgttttcta ataaaatatt tgagattcag tgagactttt
     3841 ttcttggcgg cacaataaag tttcctcttt ttgttgggtc actcaacgaa cacaatacaa
     3901 atatttgagc gctcgcattt ttctcaacct ttcgggttta aagcacttca aaatggaatt
     3961 gaagagagat tgtgaaagta catcagacag aatcacattt tgatagcatt cacttcactc
     4021 agatgcaaac ttttcagtga ctataaaaag tcataaaata ataacattaa aaaagcaaat
     4081 aaattagcga gagaattttt tgacaaaaag aaagaagagt gatagagaag aagggaatgc
     4141 ttgaaaggat tttgcattta tcttaATCAC CGTCTTCGAG AAGACGAACC ATGGCGCCAG
     4201 TTTCAGAATC CAAGTTTCTA ATATAGTTCA TCACAATGTT GACGTTGGTC CAACCTCCGG
     4261 CTTGCATAAT CTCTGGGATG GAAACTCCAG CGCGAGCCAT GTCACGGGCA GCTCCGACAC
     4321 GGGCGGAATG TCCAGACCAA GCCAAGTAAC GTTGTCCGGA ATCATCCTTG GCTCCGTAGA
     4381 TGAGACGGTG GGTAGCTTCG AAGATTCctg aaaatttatt ttcagcttat tcttagctaa
     4441 tatgttgttt aaacttacCT TCAAGAGCTC GAGTCGAGAG TTGTGATGTT GCACTCGGGG
     4501 CAGCAACTCC GTTCTTGCGA ACACGACAGA ACAGGTAGTT GTTTGGATCA TCGGCGACTC
     4561 CGGAAACCGA AATCCAGCGC TCAACGAGCT TGGTAACTCC AAGGGAGAGC GCTTTTTCGA
     4621 CTCCGGCAGT CGAGACCAGC GTTTTTGTAC GTCCGATGTG GATGAGCATT CTTCCTCCGT
     4681 CTGTGCGACT AATATCCTTG ACGCGAATGC GGGCAATTTC GGCGATACGG AGAAGTGTGT
     4741 TGTAAGCAAT TCCGAGGAAG GCGAGGTTTC GGATGTCCTG GCAACGATCG GAATTTTCCA
     4801 TAAGAGAACG GACCTGATCG AAGTCGGTTc tgaaaatttc gtcaagtctt aggattgaat
     4861 acattaatat ttaaacttac CTTTCGAAGG CGAGGGCTTG CTTGGCTCTC TCTCCAGCAT
     4921 CAACATTTTC CTTGCGGATA CGTCTCATTA CGAGAGATAC GGCATTAGAA TCTGATGGGC
     4981 GTGGAAGTCC AGATCGGCGA TGGAGCATAT TGAGCTGTCC GAGATGTTGT TGGATCGTCT
     5041 TCACCGCCAA TCCACGAGCT TGGAGGTAGA GCAAGTAGTC ACGAACGTCT TCTGGCTCGG
     5101 CTGGGAACCA CTTGCGGTTG TTAAGCTTGC ACCAGGCAGC CCAAGAACGG CACACTGAGA
     5161 GAAGCATCTT CCAGGTGTGC TCGGAGAAGG CTTGTCTGTC ACGGAACATA TCCATGAGGT
     5221 TCTTGCGAAC TTCATCACTT GTCGCGTCAA CTGGAAGTGC TGGAAGATTT TGATGAACAG
     5281 TGAGAAGATT TGACATattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct
     5341 tcctctgagc ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa
     5401 ggtcctagat gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa
     5461 atgtatctgg gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc
     5521 tccttttgca acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg
     5581 ttgaaaataa ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaaactat
     5641 aatcatgtca ctttaccact atttccgtcc agctcaacgt tccgtttttg gtgatcATTT
     5701 TTGCTTTCGT CGTAAATCTA CACACGCGTC TCTTCCGTGC GAGAGTCCAA GCCAGCAGCC
     5761 AAATTCGTTG ACTGAGTATT CAACGTTTAT ACGTTGTCGG CAACGAGAAA TAGGAAAATG
     5821 CATCGGGAAA TGTTCTTTTT TCGATTTTTT CCAAGGTTTT GACAAATTTT ACCACGAATT
     5881 TTGCTATGTT TTCAATTAAA AAATATGTTA TTCAACTGTT TCTATGAGGA AAATAAGGCT
     5941 TTGCATGTAA TTTTCTTATT CAGCATAATT TTTAATTAAT TTGAATTTTC TGTCCTAACG
     6001 TTTATTTTGT TTTCTTGGTT ATGACTGATC TGAAATTAAT TTTTGAATTT TAAGGTAATA
     6061 TGTCAGGCGG TGCCGCAAGT TTGTACAAAA AAGCAGGCTC CATGAAAAAG CCTGAACTCA
     6121 CCGCGACGTC TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC
     6181 AGCTCTCGGA GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG
     6241 TCCTGCGGGT AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT
     6301 TTGCATCGGC CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC
     6361 TGACCTATTG CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG
     6421 AACTGCCCGC TGTTCTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC
     6481 TTAGCCAGAC GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT
     6541 GGCGTGATTT CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG
     6601 ACGACACCGT CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG
     6661 ACTGCCCCGA AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG
     6721 ACAATGGCCG CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT
     6781 ACGAGGTCGC CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC
     6841 GCTACTTCGA GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC
     6901 TCCGCATTGG TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG
     6961 CTTGGGCGCA GGGTCGATGC GACGCAATCG TCCGATCCGG AGCCGGGACT GTCGGGCGTA
     7021 CACAAATCGC CCGCAGAAGC GCGGCCGTCT GGACCGATGG CTGTGTAGAA GTACTCGCCG
     7081 ATAGTGGAAA CCGACGCCCC AGCACTCGTC CGAGGGCAAA GGAATAGACC CAGCTTTCTT
     7141 GTACAAAGTG GGTCCAATTA CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC
     7201 ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT
     7261 TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA
     7321 AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA
     7381 CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT
     7441 AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT
     7501 TTTAATAACT TCGTATAGCA TACATTATAC GAAGTTATtt tcagGGAGCC GGATCTGATT
     7561 ATAAAGACGA TGACGATAAG CGTGACTACA AGGACGACGA CGACAAGCGT GATTACAAGG
     7621 ATGACGATGA CAAGAGActa gtTCGACCTG CAGACTGGCT GTGTATAAGG GAGCCTGACA
     7681 TTTATATTCC CCAGAACATC AGGTTAATGG CGTTTTTGAT GTCATTTTCG CGGTGGCTGA
     7741 GATCAGCCAC TTCTTCCCCG ATAACGGAGA CCGGCACACT GGCCATATCG GTGGTCATCA
     7801 TGCGCCAGCT TTCATCCCCG ATATGCACCA CCGGGTAAAG TTCACGGGGG ACTTTATCTG
     7861 ACAGCAGACG TGCACTGGCC AGGGGGATCA CCATCCGTCG CCCGGGCGTG TCAATAATAT
     7921 CACTCTGTAC ATCCACAAAC AGACGATAAC GGCTCTCTCT TTTATAGGTG TAAACCTTAA
     7981 ACTGCATTTC ACCAGCCCCT GTTCTCGTCG GCAAAAGAGC CGTTCATTTC AATAAACCGG
     8041 GCGACCTCAG CCATCCCTTC CTGATTTTCC GCTTTCCAGC GTTCGGCACG CAGACGACGG
     8101 GCTTCATTCT GCATGGTTGT GCTTACCGAA CCGGAGATAT TGACATCATA TATGCCTTGA
     8161 GCAACTGATA GCTGTCGCTG TCAACTGTCA CTGTAATACG CTGCTTCATA GCATACCTCT
     8221 TTTTGACATA CTTCGGGTtc ctaggaaatc gataacatgg tcatagctgt ttcctgtgtg
     8281 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8341 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8401 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8461 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8521 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8581 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8641 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8701 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8761 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8821 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8881 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8941 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9001 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9061 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9121 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9181 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9241 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9301 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9361 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9421 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9481 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9541 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9601 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9661 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9721 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9781 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9841 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9901 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9961 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10021 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10081 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10141 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10201 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10261 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10321 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10381 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    10441 attaacctat aaaaataggc gtatcacgag gccctttcgt c 
//
