LOCUS       pJJR83       10470 bp ds-DNA     circular     16-MAR-2016
DEFINITION  Cloning vector pUC19, complete sequence.
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19 other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     pDD282 backbone, GFP-C1 replaced with worm codon-optimized mCherry
COMMENT     To prepare vector for cloning: 
COMMENT     -Digest with ClaI and SpeI to fuse 5' homology arm directly to the
            tag, or 
COMMENT     -Digest with AvrII and SpeI to include a flexible linker (for
            C-terminal tags).
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    404..1010
                     /label=This sequence will be replaced by the homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(2761..2901,2949..3782)
                     /label=SQT-1
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1038..1895
                     /label=mCherry_cai_0.99
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6091..7116
                     /note="hygromycinR"
                     /label=hygR
                     /ApEinfo_fwdcolor=#006666
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1191..1241
                     /label=GFP_intr-1
                     /ApEinfo_fwdcolor=#ffdb8f
                     /ApEinfo_revcolor=#ffdb8f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1977..2006
                     /label=myc
                     /ApEinfo_fwdcolor=#6666ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1464..1514
                     /label=GFP_intr-2
                     /ApEinfo_fwdcolor=#ffdb8f
                     /ApEinfo_revcolor=#ffdb8f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        complement(8651..9203)
                     /note="RNAII transcript (complementary strand)"
                     /label=RNAII transcript (complementary strand)
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1723..1773
                     /label=GFP-intr-3
                     /ApEinfo_fwdcolor=#ffdb8f
                     /ApEinfo_revcolor=#ffdb8f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        9093..9200
                     /note="RNAI transcript"
                     /label=RNAI transcript
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(join(4155..4396,4448..4818,4870..5285))
                     /label=Cre
                     /ApEinfo_fwdcolor=#6699cc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1076..1095
                     /label=mCherry seq R 
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff9224
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8651..9239)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /label=pMB1 origin of replication (counter-clockwise) (RN
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1810..1829
                     /label=mCherry seq F
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff9224
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      9057..9062
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /label=RNAI promoter (clockwise); TTGAAG
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      complement(9234..9239)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /label=RNAII promoter (counter-clockwise); TTGAGA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     378..395
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1923..1933
                     /label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      9079..9084
                     /note="RNAI promoter (clockwise); GCTACA"
                     /label=RNAI promoter (clockwise); GCTACA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1934..1967
                     /label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      complement(9213..9218)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /label=RNAII promoter (counter-clockwise); CGTAAT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9413..10072)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1974..1976
                     /label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 3012..3012
                     /label=e1350
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9410..10270)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2010..2390
                     /label=let-858 Terminator
                     /ApEinfo_fwdcolor=#cc0000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1968..1973
                     /label=intron(1)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(8245..8265)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     5'UTR           join(2675..2702,2759..2760)
                     /label=sqt-1 5'UTR
                     /ApEinfo_fwdcolor=#ccffcc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1896..1916
                     /label=TEV site
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    661..966
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8633..9315)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           3783..3819
                     /label=sqt-1 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(5286..5685)
                     /label=hsp16-41 promoter
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7600..7626
                     /label=Flag
                     /ApEinfo_fwdcolor=#ff6699
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2391..2760
                     /label=Psqt1
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             754..963
                     /label=ccdB(1)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7573..7599
                     /label=Flag(1)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7534..7545
                     /label=spacer(1)
                     /ApEinfo_label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7546..7572
                     /label=Flag(2)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#cc3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           complement(3820..4151)
                     /label=tbb-2 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#993333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          7528..7533
                     /label=intron(2)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           7142..7493
                     /label=unc-54 3'UTR
                     /ApEinfo_fwdcolor=#990000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7494..7527
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        5686..6048
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;
                     /ApEinfo_revcolor=pink; /ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
                     /label=Prps-0
                     /ApEinfo_fwdcolor=#339933
                     /ApEinfo_revcolor=#339933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8234..8240
                     /label=This sequence will be deleted if ClaI is used
                     /ApEinfo_fwdcolor=#ffcc99
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7627..8233
                     /label=This sequence will be replaced by the homology
                     arm(1)
                     /ApEinfo_label=This sequence will be replaced by the
                     homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(7671..7976)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB(2)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(7674..7883)
                     /label=ccdB(3)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_structure  1011..1037
                     /label=Flexible Linker (will be deleted if ClaI is used)
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtTA CCCGAAGTAT
      421 GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT
      481 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTTC GGTAAGCACA ACCATGCAGA
      541 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG
      601 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCCGA CGAGAACAGG GGCTGGTGAA
      661 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA
      721 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT
      781 CTGCTGTCAG ATAAAGTCCC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC
      841 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG
      901 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA
      961 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGAcctag GGAGCATCGG
     1021 GAGCCTCAGG AGCATCGATG TCCAAGGGAG AGGAGGACAA CATGGCCATC ATCAAGGAGT
     1081 TCATGCGTTT CAAGGTCCAC ATGGAGGGAT CAGTCAACGG ACACGAGTTC GAGATCGAGG
     1141 GAGAGGGAGA GGGACGTCCA TACGAGGGAA CCCAAACCGC CAAGCTCAAG gtaagtttaa
     1201 acatatatat actaactaac cctgattatt taaattttca gGTCACCAAG GGAGGACCAC
     1261 TCCCATTCGC CTGGGACATC CTCTCCCCAC AATTCATGTA CGGATCAAAG GCCTACGTCA
     1321 AGCACCCAGC CGACATCCCA GACTACCTCA AGCTCTCCTT CCCAGAGGGA TTCAAGTGGG
     1381 AGCGTGTCAT GAACTTCGAG GACGGAGGAG TCGTCACCGT CACCCAAGAC TCCTCCCTCC
     1441 AAGACGGAGA GTTCATCTAC AAGgtaagtt taaacagttc ggtactaact aaccatacat
     1501 atttaaattt tcagGTCAAG CTCCGTGGAA CCAACTTCCC ATCCGACGGA CCAGTCATGC
     1561 AAAAGAAGAC CATGGGATGG GAGGCCTCCT CCGAGCGTAT GTACCCAGAG GACGGAGCCC
     1621 TCAAGGGAGA GATCAAGCAA CGTCTCAAGC TCAAGGACGG AGGACACTAC GACGCCGAGG
     1681 TCAAGACCAC CTACAAGGCC AAGAAGCCAG TCCAACTCCC AGgtaagttt aaacatgatt
     1741 ttactaacta actaatctga tttaaatttt cagGAGCCTA CAACGTCAAC ATCAAGCTCG
     1801 ACATCACCTC CCACAACGAG GACTACACCA TCGTCGAGCA ATACGAGCGT GCCGAGGGAC
     1861 GTCACTCCAC CGGAGGAATG GACGAGCTCT ACAAGGAGAA TCTGTACTTT CAATCCGGAA
     1921 AGgtaagttt aaaATAACTT CGTATAGCAT ACATTATACG AAGTTATttt cagGGAGAGC
     1981 AAAAGCTTAT CTCCGAGGAG GACCTCTAAG GATGATCGAC GCCaACGTCG TTGAATTTTC
     2041 AAATTTTAAA TACTGAATAT TTGTTTTTTT TCCTATTATT TATTTATTCT CTTTGTGTTT
     2101 TTTTTCTTGC TTTCTAAAAA ATTAATTCAA TCCAAATCTA Aacatttttt tttctctttc
     2161 cgtctcccaa ttcgtattcc gctcctctca tctgaacaca atgtgcaagt ttatttatct
     2221 tctcgctttc atttcattag gacgtggggg gaattggtgg aagggggaaa cacacaaaag
     2281 gatgatggaa atgaaataag gacacacaat atgcaacaac attcaattca gaaatatgga
     2341 ggaaggttta aaagaaaaca taaaaatata tagaggagga aggaaactag ctgccctcga
     2401 ttgtctatct tccattctca ccaagtttca acaactttat tcagtcattc ttctctggtt
     2461 catttcccaa ccaatcttcc cgaataaagc ccatctgtcc tttcggccac attcctcacc
     2521 tacatccgta ctccttatct cccgttctta tccagtcttt tatcgtctct gcgtctcgca
     2581 cactgtctcg tcggtaatgt aaaaaaaaaa caaaaaaaac ttgaaaactt gcggaaagat
     2641 ttcctaataa aaggagacga atccggtagg aaatcgacaa gtctctgctt ctcgaagact
     2701 aggtgagtta catttctttg gccgaattat tttgaaatcc taaatttgat aatttcagag
     2761 ATGTCTGTAA AACTTGCGTG TTATGTGACG GCTTCGGTCA CTGTCGCCAC TCTTATGGTA
     2821 TGCTTCATGA CCATGTCAAC CATCTACTCG GAGGTTGATG GATTCAGAGA GAAGCTTGAT
     2881 ACCGAGATGA ATGTGTTCAG Agtgagttaa attatatttt gaattttaaa taatttttaa
     2941 ttttctagCA ATCTACCAAT GGATTGTGGA AGGACATAGT TGTCATCGGA AGATCTAGCA
     3001 AGCGTGTCCG TTGTCAATAT GAAGAGACCA ACGCTACCCC AACTCCACAT GCTGATGGAT
     3061 CCCCATCTGC TCCACCAGGT CAACCACCAG CAGTTCCACC AGTCTTCAAC CAGCCAAAGA
     3121 CTCCAAATGG AGCCAATGGA AATGGACCAA CCTGCAACTG CAATGCTGAT AACAAGTGCC
     3181 CAGCTGGACC ATCCGGACCA AAGGGAGTTC CAGGAGTTCC AGGACTCGAC GGAGTTCCAG
     3241 GACTTGACGG TGTTCCAGGA GTTGGAGCTG ATGATATCGC TCCACAACGC GAGTCTGTCG
     3301 GATGCTTCAC TTGCCCACAA GGACCAGTTG GACCACCAGG AGCTCTTGGA AGACCAGGAC
     3361 CACGTGGACT TCCAGGACCA AGAGGACAAA ATGGAAACCC AGGAAGAGAT GGACAACCAG
     3421 GACATCCAGG AGAGCAAGGA TCATCCGGTC AAATCGGAAA GATCGGAGAG CCAGGACCAC
     3481 CAGGAGAGAA GGGACGCGAC GCCGAGCATC CAATCGGAAG ACCAGGACCA AAGGGACCAA
     3541 GAGGAGATCA AGGACCAACA GGACCAGCTG GACAGAACGG TCTTCACGGA CCACCAGGAG
     3601 AGCCAGGAAC CGTTGGACCA GAAGGACCAT CTGGAAAGCA AGGACGTCAA GGACCAGACG
     3661 GAACCCAGGG AGAGACTGGA CCAGACGGAA GACCAGGAAA GGATGCCGAG TACTGCCAGT
     3721 GCCCAGACAA GTCTCCACCA TCAGAGGCTG TCAACGCCAA CCGTGGATAC AGAAATATCT
     3781 AAattgttgg tgttttctaa taaaatattt gagattcagt gagacttttt tcttggcggc
     3841 acaataaagt ttcctctttt tgttgggtca ctcaacgaac acaatacaaa tatttgagcg
     3901 ctcgcatttt tctcaacctt tcgggtttaa agcacttcaa aatggaattg aagagagatt
     3961 gtgaaagtac atcagacaga atcacatttt gatagcattc acttcactca gatgcaaact
     4021 tttcagtgac tataaaaagt cataaaataa taacattaaa aaagcaaata aattagcgag
     4081 agaatttttt gacaaaaaga aagaagagtg atagagaaga agggaatgct tgaaaggatt
     4141 ttgcatttat cttaATCACC GTCTTCGAGA AGACGAACCA TGGCGCCAGT TTCAGAATCC
     4201 AAGTTTCTAA TATAGTTCAT CACAATGTTG ACGTTGGTCC AACCTCCGGC TTGCATAATC
     4261 TCTGGGATGG AAACTCCAGC GCGAGCCATG TCACGGGCAG CTCCGACACG GGCGGAATGT
     4321 CCAGACCAAG CCAAGTAACG TTGTCCGGAA TCATCCTTGG CTCCGTAGAT GAGACGGTGG
     4381 GTAGCTTCGA AGATTCctga aaatttattt tcagcttatt cttagctaat atgttgttta
     4441 aacttacCTT CAAGAGCTCG AGTCGAGAGT TGTGATGTTG CACTCGGGGC AGCAACTCCG
     4501 TTCTTGCGAA CACGACAGAA CAGGTAGTTG TTTGGATCAT CGGCGACTCC GGAAACCGAA
     4561 ATCCAGCGCT CAACGAGCTT GGTAACTCCA AGGGAGAGCG CTTTTTCGAC TCCGGCAGTC
     4621 GAGACCAGCG TTTTTGTACG TCCGATGTGG ATGAGCATTC TTCCTCCGTC TGTGCGACTA
     4681 ATATCCTTGA CGCGAATGCG GGCAATTTCG GCGATACGGA GAAGTGTGTT GTAAGCAATT
     4741 CCGAGGAAGG CGAGGTTTCG GATGTCCTGG CAACGATCGG AATTTTCCAT AAGAGAACGG
     4801 ACCTGATCGA AGTCGGTTct gaaaatttcg tcaagtctta ggattgaata cattaatatt
     4861 taaacttacC TTTCGAAGGC GAGGGCTTGC TTGGCTCTCT CTCCAGCATC AACATTTTCC
     4921 TTGCGGATAC GTCTCATTAC GAGAGATACG GCATTAGAAT CTGATGGGCG TGGAAGTCCA
     4981 GATCGGCGAT GGAGCATATT GAGCTGTCCG AGATGTTGTT GGATCGTCTT CACCGCCAAT
     5041 CCACGAGCTT GGAGGTAGAG CAAGTAGTCA CGAACGTCTT CTGGCTCGGC TGGGAACCAC
     5101 TTGCGGTTGT TAAGCTTGCA CCAGGCAGCC CAAGAACGGC ACACTGAGAG AAGCATCTTC
     5161 CAGGTGTGCT CGGAGAAGGC TTGTCTGTCA CGGAACATAT CCATGAGGTT CTTGCGAACT
     5221 TCATCACTTG TCGCGTCAAC TGGAAGTGCT GGAAGATTTT GATGAACAGT GAGAAGATTT
     5281 GACATatttt cgaagttttt tagatgcact agaacaaagc gtgttggctt cctctgagcc
     5341 cgctttcctt atatacccgc attctgcagc cttacagaat gttctagaag gtcctagatg
     5401 cattcgtttg aaaatactcc cggtgggtgc aaagagacgc agacggaaaa tgtatctggg
     5461 tctctttatt gtgtacacta cttttccatg taccgaatgt gagtcgccct ccttttgcaa
     5521 caagcagctc gaatgttcta gaaaaaggtg gaaaatagta taaataccgt tgaaaataaa
     5581 taccgaacaa catttgctct aattgtgaaa ttagaaatct tcaaactata atcatgtcac
     5641 tttaccacta tttccgtcca gctcaacgtt ccgtttttgg tgatcATTTT TGCTTTCGTC
     5701 GTAAATCTAC ACACGCGTCT CTTCCGTGCG AGAGTCCAAG CCAGCAGCCA AATTCGTTGA
     5761 CTGAGTATTC AACGTTTATA CGTTGTCGGC AACGAGAAAT AGGAAAATGC ATCGGGAAAT
     5821 GTTCTTTTTT CGATTTTTTC CAAGGTTTTG ACAAATTTTA CCACGAATTT TGCTATGTTT
     5881 TCAATTAAAA AATATGTTAT TCAACTGTTT CTATGAGGAA AATAAGGCTT TGCATGTAAT
     5941 TTTCTTATTC AGCATAATTT TTAATTAATT TGAATTTTCT GTCCTAACGT TTATTTTGTT
     6001 TTCTTGGTTA TGACTGATCT GAAATTAATT TTTGAATTTT AAGGTAATAT GTCAGGCGGT
     6061 GCCGCAAGTT TGTACAAAAA AGCAGGCTCC ATGAAAAAGC CTGAACTCAC CGCGACGTCT
     6121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG
     6181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA
     6241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC
     6301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC
     6361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT
     6421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG
     6481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC
     6541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC
     6601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA
     6661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC
     6721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC
     6781 AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG CTACTTCGAG
     6841 CGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT CCGCATTGGT
     6901 CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG ATGATGCAGC TTGGGCGCAG
     6961 GGTCGATGCG ACGCAATCGT CCGATCCGGA GCCGGGACTG TCGGGCGTAC ACAAATCGCC
     7021 CGCAGAAGCG CGGCCGTCTG GACCGATGGC TGTGTAGAAG TACTCGCCGA TAGTGGAAAC
     7081 CGACGCCCCA GCACTCGTCC GAGGGCAAAG GAATAGACCC AGCTTTCTTG TACAAAGTGG
     7141 GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT
     7201 TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT
     7261 CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA
     7321 AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT
     7381 GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG
     7441 CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTAATAACTT
     7501 CGTATAGCAT ACATTATACG AAGTTATttt cagGGAGCCG GATCTGATTA TAAAGACGAT
     7561 GACGATAAGC GTGACTACAA GGACGACGAC GACAAGCGTG ATTACAAGGA TGACGATGAC
     7621 AAGAGActag tTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC
     7681 CAGAACATCA GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT
     7741 TCTTCCCCGA TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT
     7801 TCATCCCCGA TATGCACCAC CGGGTAAAGT TCACGGGGGA CTTTATCTGA CAGCAGACGT
     7861 GCACTGGCCA GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA
     7921 TCCACAAACA GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA
     7981 CCAGCCCCTG TTCTCGTCGG CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC
     8041 CATCCCTTCC TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG
     8101 CATGGTTGTG CTTACCGAAC CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG
     8161 CTGTCGCTGT CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC
     8221 TTCGGGTtcc taggaaatcg ataacatggt catagctgtt tcctgtgtga aattgttatc
     8281 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8341 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8401 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8461 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8521 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8581 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8641 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8701 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8761 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8821 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8881 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8941 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9001 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9061 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9121 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9181 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9241 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9301 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9361 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9421 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9481 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9541 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9601 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9661 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9721 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9781 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9841 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9901 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9961 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10021 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10081 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10141 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10201 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10261 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10321 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10381 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    10441 aaaataggcg tatcacgagg ccctttcgtc   
//
