LOCUS       FDHDNF_7_DD4182        6191 bp DNA     circular SYN 12-JUN-2024
DEFINITION  DD4182.
ACCESSION   DD4182
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2024 from SnapGene Viewer 6.2.0
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..6191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     CDS             772..824
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=4.9"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="APNTQTASPRALADSLM"
     LTR             complement(890..1359)
                     /label=LTR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: LEFT"
     LTR             complement(1360..1481)
                     /label=3' LTR (Delta-U3) (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.6"
                     /note="/match_length=28.9"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: LEFT"
     CDS             complement(1530..2249)
                     /codon_start=1
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(1533..2249)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="This feature has 3 segments:
                        1: 1533 .. 2243 / #05fc14
                        2: 2244 .. 2246 / #05fc14 / 1a
                        3: 2247 .. 2249 / #05fc14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    2250..2837
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 2250 .. 2258 / #a6acb3
                        2: 2259 .. 2261 / #a6acb3 / ATG
                        3: 2262 .. 2837 / #a6acb3"
     misc_feature    complement(2263..2725)
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        2834..2850
                     /label=chicken beta-actin promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.6"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     CDS             complement(2872..3093)
                     /codon_start=1
                     /label=UBIQ_CERCA
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=91.9"
                     /note="/match_length=97.4"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MLIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLAF
                     AGKSLEDGRTLSDYNILKDSKLHLVLRLR"
     CDS             complement(3103..3129)
                     /codon_start=1
                     /label=3xHA (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=33.3"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="YPYDVPDYA"
     misc_feature    complement(3161..3535)
                     /label=pol region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(3545..3961)
                     /codon_start=1
                     /label=gag (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    4020..4377
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
                     /note="color: #ffe4c4"
     misc_feature    complement(4038..4126)
                     /label=MESV Psi (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.9"
                     /note="/match_length=26.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(4178..4377)
                     /label=MMLV Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     LTR             complement(4440..5033)
                     /label=LTR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: LEFT"
     promoter        5063..5167
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5168..6028
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg gccatttaat
      841 gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt gaaagacccc
      901 cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga gaccacaagt
      961 cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact cagtcaatcg
     1021 gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg ggagcagaag
     1081 cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca cagggtcatt
     1141 tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc tgagggctgg
     1201 accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg cttaccacag
     1261 atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag ccggggcagg
     1321 aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct gttcctgacc
     1381 ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa aatggcgtta
     1441 cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt ttctggagac
     1501 taaataaaat cttttatttt atcgtcgact tacttgtaca gctcgtccat gccgagagtg
     1561 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     1621 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     1681 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
     1741 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
     1801 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     1861 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     1921 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     1981 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
     2041 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     2101 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
     2161 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     2221 gtgaacagct cctcgccctt gctcaccatg gttgtggcca tattatcatc gtgtttttca
     2281 aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc tcgactaaac
     2341 acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg gtaccttctg
     2401 ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc tttccacaac
     2461 tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat cttatacacg
     2521 tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt
     2581 cgctacagac gttgtttgtc ttcaagaagc ttccagagga actgcttcct tcacgacatt
     2641 caacagacct tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa
     2701 gacagggcca ggtttccggg ccctcacatt gccaaaagac ggcaatatgg tggaaaataa
     2761 catatagaca aacgcacacc ggccttattc caagcggctt cggccagtaa cgttaggggg
     2821 ggggggaggg agaggggggg gggggggcgg aatttacgta gcggccgctc aacgaagtct
     2881 caacactaga tgaagtttag agtcctttag aatattgtag tcagacaaag tacgtccatc
     2941 ttccagcgat ttgccagcaa aggccagtct ctgctgatca ggaggaattc cttccttatc
     3001 ctggatcttg gcctttacat tttctatcgt atccgagggt tcaacctcga gggtgatggt
     3061 tttcccggta agggttttca cgaaaatcaa cattgcagct gcagcgtaat ctggaacatc
     3121 gtatgggtac atggtggctt aattaactag ctagatccgg cagtctagag gatggtccac
     3181 ccccggggtc ggcagccttc acgtgggcgg cgtgtatcca agctgcgatg ccgtctactt
     3241 tgagggcggt gggggtggtc agcaggactg tgtaaggtcc tttccagcga ggttctaggt
     3301 tcttagtctg gtgtcggcgg acccacactg tgtcgccgac tcggtaaggg tgaggtacca
     3361 ccggtcggtc cagttgttct tggtaggctg ccgccagagg tctccagact tcgtgctgga
     3421 ctaagtagag agcctgtaag tgagcttgga gagaggggct gttagtaact cttgtcatgt
     3481 cagggtcagg gaagtttaca aggggcgggg gtgccccata taagatctca tatggccata
     3541 tgggggcgcc tagagaagga gtgagggctg gataaaggga ggatcgaggc ggggtcgaac
     3601 gaggaggttc aagggggaga gacggggcgg atggaggaag aggaggcgga ggcttagggt
     3661 gtacaaaggg cttgacccag ggaggggggt caaaagccaa ggcttcccag gtcacgatgt
     3721 aggggacctg gtctgggtgt ccatgcgggc caggtgaaaa gaccttgatc ttaacctggg
     3781 tgatgaggtc tcggttaaag gtgccgtctc gcggccatcc gacgttaaag gttggccatt
     3841 ctgcagagca gaaggtaacc caacgtctct tcttgacatc taccgactgg ttgtgagcga
     3901 tccgctcgac atctttccag tgacctaagg tcaaacttaa gggagtggta acagtctggc
     3961 cctaattttc agacaaatac agaaacacag tcagacagag acaacacaga acgatgctgc
     4021 agcagacaag acgcgcggcg cggcttcggt cccaaaccga aagcaaaaat tcagacggag
     4081 gcgggaactg ttttaggttc tcgtctccta ccagaaccac atatccctcc tattaggggg
     4141 gtgcaccaaa gagtccaaaa cgatcgggat ttttggactc aggtcgggcc acaaaaacgg
     4201 cccccgaagt ccctgggacg tctcccaggg ttgcggccgg gtgttccgaa ctcgtcagtt
     4261 ccaccacggg tccgccagat acagagctag ttagctaact agtaccgacg caggcgcata
     4321 aaatcagtca tagacactag acaatcggac agacacagat aagttgctgg ccagcttacc
     4381 tcccggtggt gggtcggtgg tccctgggca ggggtctccc gatcccggac gagcccccaa
     4441 atgaaagacc cccgctgacg ggtagtcaat cactcagagg agaccctccc aaggaacagc
     4501 gagaccacaa gtcggatgca actgcaagag ggtttattgg atacacgggt acccgggcga
     4561 ctcagtcaat cggaggactg gcgcgccgag tgaggggttg tgggctcttt tattgagctc
     4621 ggggagcaga agcgcgcgaa cagaagcgag aagcgaactg attggttagt tcaaataagg
     4681 cacagggtca tttcaggtcc ttggggcacc ctggaaacat ctgatggttc tctagaaact
     4741 gctgagggct ggaccgcatc tggggaccat ctgttcttgg ccctgagccg gggcaggaac
     4801 tgcttaccac agatatcctg tttggcccat attcagctgt tccatctgtt cttggccctg
     4861 agccggggca ggaactgctt accacagata tcctgtttgg cccatattca gctgttccat
     4921 ctgttcctga ccttgatctg aacttttcta ttctcagtta tgtatttttc catgccttgc
     4981 aaaatggcgt tacttaagct agcttgccaa acctacaggt ggggtctttc attatgcagg
     5041 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5101 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5161 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5221 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5281 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5341 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5401 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5461 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5521 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5581 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5641 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5701 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5761 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5821 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5881 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5941 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6001 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6061 gattgatttg cggccggccg caaaaggatc taggtgaaga tcctttttga taatctcatg
     6121 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6181 aaaggatctt c
//
