LOCUS       pZA21MCS                4822 bp ss-DNA     circular SYN 02-Feb-2011
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     variation       1..6
                     /note="XhoI"
     promoter        7..292
                     /note="PBAD promoter"
     prim_transcript 292
                     /note="+1 transcription start site"
     variation       293..298
                     /note="EcoRI"
     variation       314..319
                     /note="KpnI"
     CDS             320..2683
                     /note="mf-lon"
     variation       2684..2689
                     /note="HindIII"
     terminator      2715..2818
                     /note="T1 terminator"
     variation       2819..2824
                     /note="AvrII"
     rep_origin      2825..3620
                     /note="P15A origin"
     variation       3621..3626
                     /note="SpeI"
     terminator      3627..3732
                     /note="T0 terminator"
     variation       3733..3738
                     /note="SacI"
     gene            complement(3739..4747)
                     /note="ampR with promoter"
     variation       4748..4753
                     /note="AatII"
BASE COUNT      1630 A       918 C       962 G      1310 T         0 OTHER
ORIGIN      ?
        1 CTCGAGAAGA AACCAATTGT CCATATTGCA TCAGACATTG CCGTCACTGC GTCTTTTACT
       61 GGCTCTTCTC GCTAACCAAA CCGGTAACCC CGCTTATTAA AAGCATTCTG TAACAAAGCG
      121 GGACCAAAGC CATGACAAAA ACGCGTAACA AAAGTGTCTA TAATCACGGC AGAAAAGTCC
      181 ACATTGATTA TTTGCACGGC GTCACACTTT GCTATGCCAT AGCATTTTTA TCCATAAGAT
      241 TAGCGGATCC TACCTGACGC TTTTTATCGC AACTCTCTAC TGTTTCTCCA TAGAATTCAT
      301 TAAAGAGGAG AAAGGTACCA TGAGTAAAAA AATAAAACTA CCTATTTTCC AAATACGCGG
      361 TTCATTTATT GTTCCTGGAA TTAAAGAGAA TTTAGAAGTT GGAAGAAAAA ACACATTAGC
      421 GAGCGTTAAT TATGCGATTA AAAATTCAAA TAATCAAATG ATCGCAATAC CGCAGATTGA
      481 TGCATCTGTT GAAAAACCTG AATTTTCAGA TTTACATGAA TTTGGAATTT TAATTGATTT
      541 TGAAGTAATC AAAGAATGGA AAGATAATTC ATTAACTATT AGCACAAACC CTATTCAACG
      601 ATGTAAAGTA ATTAGCTTTT TTGAAAATGA AGATCAAGTG CCGTATGCGG AAGTTGAATT
      661 AATTGAATCA ATTAATGATT TTAGTGATGA AGAGTTAAAA GAATTAATTG AAAAAATCTC
      721 TGATGCTATC AAAACTAAAG CTAGTTTAGT AACAAAACAA ATTAAACAGT TAATATCAGG
      781 TGAATCTGAT GATTTAAGTT TAGCTTTTGA TTCAATTATG TTTAAACTGG CTCCGTCAAA
      841 AATATTGACA AATCCGGAAT ACATAACATC TCCGTCATTA AAAACACGAT GGTCAATTAT
      901 TGAAAAAATA ATTTTTGCTG AAGATGGAAT CATAACAAGA AATGCAGAAT CAATTGATGC
      961 AGCACGACAA AAGAATGAAA TTGAACAAGA ACTGAACCAT AAATTAAAAG AAAAAATGGA
     1021 TAAACAACAA AAAGAATATT ATCTGCGAGA AAAAATGCGA ATTATCAAAG ATGAATTAGA
     1081 AGATGAAGAT GATTCTGATG ACAGTTCATT AGAAAAATAT AAAGAACGTT TAGCTAAAGA
     1141 ACCTTTCCCT GAAGAAGTCA AACGAAAAAT TATGGCTTCT ATCAAACGAG TTGAAGCACT
     1201 TCAATCAGGA ACTCCTGAAT GGAATACTGA GAAAAATTAC ATTGATTGGA TGATGAGTAT
     1261 TCCCTGGTGG GAAGAAACTG AAGACTTAAC TGATTTAAAA TATGCTAAAA AAATTTTAGA
     1321 CAAACATCAT TATGGTATGA AAAAAGTTAA AGAAAGAATT ATTGAGTACT TAGCTGTTAA
     1381 AACTAAAACA AAATCACTAA AAGCACCAAT TATTACATTA GTTGGTCCCC CAGGTGTAGG
     1441 TAAAACAAGT TTAGCAAAAT CAATTGCAGA AGCAGTTGGA AAAAACTTTG TTAAAGTAAG
     1501 TTTAGGTGGA GTTAAAGATG AATCAGAAAT TCGTGGACAT AGAAAAACTT ATGTTGGTTC
     1561 AATGCCAGGA AGAATTATTC AAACAATGAA AAGAGCAAAA GTAAAAAACC CATTATTCTT
     1621 GCTTGATGAA ATTGATAAAA TGGCATCTGA TCACAGAGGT GATCCAGCAA GTGCAATGTT
     1681 AGAGGTTCTT GACCCAGAAC AAAACAAAGA GTTTTCAGAT CATTATATTG AAGAACCATA
     1741 TGATCTAAGC CAAGTTATGT TTATAGCAAC TGCTAACTAT CCTGAAGATA TTCCTGAAGC
     1801 CTTATATGAC CGTATGGAAA TCATAAATTT ATCTAGCTAT ACTGAAATTG AAAAAGTTAA
     1861 GATAGCACAA GATTATTTAG TTCCAAAAGC AATAGAACAA CATGAATTAA CTTCAGAAGA
     1921 AATATCATTT ACTGAAGGTG CAATTAATGA AATTATTAAA TATTACACTA GAGAAGCTGG
     1981 TGTGCGTCAA TTAGAAAGAC ATATTAATTC TATTATTAGA AAGTATATTG TTAAAAATCT
     2041 AAATGGTGAA ATGGATAAAA TTGTTATTGA TGAAAAACAA GTAAATGATT TATTAGGTAA
     2101 AAGAATTTTT GATCACACTG AAAAACAAGA AGAATCTCAA ATTGGTGTGG TTACAGGTTT
     2161 AGCATATACA CAATTTGGTG GAGATATTTT ACCAATTGAA GTAAGTTTAT ACCCTGGTAA
     2221 AGGAAATTTA ATTCTTACAG GTAAACTTGG TGAAGTTATG AAAGAATCTG CAACAATTGC
     2281 GTTAACTTAT GTTAAATCAA ACTTTGAAAA ATTTGGAGTA GATAAAAAAG TATTTGAAGA
     2341 AAATGATATT CATGTTCACG TTCCTGAAGG AGCTGTTCCA AAAGATGGAC CAAGTGCTGG
     2401 TATCACAATT ACAACTGCTT TAATTTCTGC ACTTTCAGAT AAACCAGTTT CAAAAGAAAT
     2461 TGGAATGACT GGTGAAATCA CACTAAGAGG AAATGTATTG CCAATTGGTG GATTAAGAGA
     2521 AAAATCAATT TCTGCTTCAA GAAGTGGATT GAAAACAATC ATTATTCCAA AGAAAAATGA
     2581 AAGAGACTTA GACGAAATTC CAGATGAAGT AAAAGCAAAA CTAAAAATTA TACCTGCTGA
     2641 AAAATATGAA GAGGTATTTG CAATAGTTTT TAAAACAAAA TAATAAGCTTG ATGGGGGATC
     2701 CCATGGTACG CGTGCTAGAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT
     2761 TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC GCCCTAGACC
     2821 TAGGGATATA TTCCGCTTCC TCGCTCACTG ACTCGCTACG CTCGGTCGTT CGACTGCGGC
     2881 GAGCGGAAAT GGCTTACGAA CGGGGCGGAG ATTTCCTGGA AGATGCCAGG AAGATACTTA
     2941 ACAGGGAAGT GAGAGGGCCG CGGCAAAGCC GTTTTTCCAT AGGCTCCGCC CCCCTGACAA
     3001 GCATCACGAA ATCTGACGCT CAAATCAGTG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     3061 CCAGGCGTTT CCCCCTGGCG GCTCCCTCGT GCGCTCTCCT GTTCCTGCCT TTCGGTTTAC
     3121 CGGTGTCATT CCGCTGTTAT GGCCGCGTTT GTCTCATTCC ACGCCTGACA CTCAGTTCCG
     3181 GGTAGGCAGT TCGCTCCAAG CTGGACTGTA TGCACGAACC CCCCGTTCAG TCCGACCGCT
     3241 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGA AAGACATGCA AAAGCACCAC
     3301 TGGCAGCAGC CACTGGTAAT TGATTTAGAG GAGTTAGTCT TGAAGTCATG CGCCGGTTAA
     3361 GGCTAAACTG AAAGGACAAG TTTTGGTGAC TGCGCTCCTC CAAGCCAGTT ACCTCGGTTC
     3421 AAAGAGTTGG TAGCTCAGAG AACCTTCGAA AAACCGCCCT GCAAGGCGGT TTTTTCGTTT
     3481 TCAGAGCAAG AGATTACGCG CAGACCAAAA CGATCTCAAG AAGATCATCT TATTAATCAG
     3541 ATAAAATATT ACTAGATTTC AGTGCAATTT ATCTCTTCAA ATGTAGCACC TGAAGTCAGC
     3601 CCCATACGAT ATAAGTTGTT ACTAGTGCTT GGATTCTCAC CAATAAAAAA CGCCCGGCGG
     3661 CAACCGAGCG TTCTGAACAA ATCCAGATGG AGTTCTGAGG TCATTACTGG ATCTATCAAC
     3721 AGGAGTCCAA GCGAGCTCGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     3781 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
     3841 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     3901 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     3961 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     4021 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     4081 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     4141 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     4201 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     4261 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     4321 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     4381 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     4441 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     4501 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     4561 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     4621 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     4681 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
     4741 GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT
     4801 CACGAGGCCC TTTCGTCTTC AC
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