LOCUS       pZE21MCS                2988 bp ss-DNA     circular SYN 01-Feb-2011
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     variation       1..6
                     /note="XhoI"
     promoter        7..63
                     /note="placIQ prom"
     -35_signal      9..14
                     /note="-35 region"
     -10_signal      32..37
                     /note="-10 region"
     variation       64..69
                     /note="EcoRI"
     variation       85..90
                     /note="KpnI"
     CDS             91..804
                     /note="gfp (mut3b)"
     variation       805..885
                     /note="pdt#3"
     variation       886..888
                     /note="stop codon"
     variation       890..895
                     /note="HindIII"
     variation       902..907
                     /note="BamHI"
     terminator      927..1030
                     /note="T1 terminator"
     variation       1031..1036
                     /note="AvrII"
     rep_origin      1037..1845
                     /note="colE1"
     variation       1846..1851
                     /note="SpeI"
     terminator      1852..1957
                     /note="T0 terminator"
     variation       1958..1963
                     /note="SacI"
     CDS             complement(1983..2777)
                     /note="kanR (kanamycin resistance)"
     variation       2914..2919
                     /note="AatII"
BASE COUNT       804 A       776 C       747 G       659 T         0 OTHER
ORIGIN      ?
        1 CTCGAGTGGT GCAAAACCTT TCGCGGTATG GCATGATAGC GCCCGGAAGA GAGTCAATTC
       61 AGGGAATTCA TTAAAGAGGA CAAAGGTACC ATGCGTAAAG GAGAAGAACT TTTCACTGGA
      121 GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT
      181 GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT
      241 GGAAAACTAC CTGTTCCATG GCCAACACTT GTCACTACTT TCGGTTATGG TGTTCAATGC
      301 TTTGCGAGAT ACCCAGATCA TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA
      361 GGTTACGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACGTGCT
      421 GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG TATTGATTTT
      481 AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC ACACAATGTA
      541 TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTA ACTTCAAAAT TAGACACAAC
      601 ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT
      661 GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT
      721 CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA
      781 CATGGCATGG ATGAACTATA CAAAGCGGCG AACAAAAACG AAGAAAACAC CAACGAAGTG
      841 CCGACCTTTA TGCTGAACGC GGGCCAGGCG AACAGAAGAC GAGTTTAATA AGCTTGATGG
      901 GGGATCCCAT GGTACGCGTG CTAGAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC
      961 TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG
     1021 CCGCCCTAGA CCTAGGGCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA
     1081 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
     1141 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
     1201 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
     1261 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
     1321 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
     1381 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
     1441 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
     1501 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
     1561 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
     1621 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
     1681 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
     1741 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
     1801 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGACTAG TGCTTGGATT
     1861 CTCACCAATA AAAAACGCCC GGCGGCAACC GAGCGTTCTG AACAAATCCA GATGGAGTTC
     1921 TGAGGTCATT ACTGGATCTA TCAACAGGAG TCCAAGCGAG CTCTCGAACC CCAGAGTCCC
     1981 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG
     2041 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA
     2101 CGGGTAGCCA ACGCTATGTC CTGATAGCGG TCCGCCACAC CCAGCCGGCC ACAGTCGATG
     2161 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC
     2221 ACGACGAGAT CCTCGCCGTC GGGCATGCGC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC
     2281 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA
     2341 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA
     2401 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG
     2461 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT
     2521 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC
     2581 CGCGCTGCCT CGTCCTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA
     2641 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT
     2701 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT
     2761 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCAGA TCTTGATCCC
     2821 CTGCGCCATC AGATCCTTGG CGGCAAGAAA GCCATCCAGT TTACTTTGCA GGGCTTCCCA
     2881 ACCTTACCAG AGGGCGCCCC AGCTGGCAAT TCCGACGTCT AAGAAACCAT TATTATCATG
     2941 ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTTCAC
//
