LOCUS       pCas9-VRER_2A_GF        9271 bp    DNA     circular     13-JUL-2015
DEFINITION  1.13.
SOURCE      .
  ORGANISM  .
COMMENT     
            ApEinfo:methylated:1
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|738430519|
COMMENT     VNTDBDATE|739366106|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCas9-VRER_2A_GFP|
COMMENT     VNTAUTHORNAME|DP|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTUDF|Freezer Position|1|F23|
FEATURES             Location/Qualifiers
     primer_bind     complement(5135..5155)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="28"
                     /label=M13-rev
     CDS             1..4137
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="4"
                     /label=Cas9
     misc_feature    4138..4206
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=T2A
     CDS             4207..4926
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="4"
                     /label=EGFP
     promoter        7549..9162
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="29"
                     /label=CAG\promoter
     rep_origin      complement(5523..6205)
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="33"
                     /label=ColE1\origin
     misc_binding    complement(5161..5183)
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="20"
                     /label=LacO
     CDS             complement(6303..6962)
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="4"
                     /label=AmpR
     polyA_signal    4971..5092
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="25"
                     /label=SV40PolyA
     misc_feature    4105..4137
                     /vntifkey="21"
                     /label=NLS
     misc_feature    4003..4005
                     /vntifkey="21"
                     /label=R1335E
     misc_feature    4009..4011
                     /vntifkey="21"
                     /label=T1337R
     misc_feature    3652..3654
                     /vntifkey="21"
                     /label=G1218R
     misc_feature    3652..3652
                     /vntifkey="21"
                     /label=G>C
     misc_feature    4003..4004
                     /vntifkey="21"
                     /label=CG>GA
     misc_feature    4009..4010
                     /vntifkey="21"
                     /label=AC>CG
     misc_feature    3403..3405
                     /vntifkey="21"
                     /label=D1135V
     misc_feature    3404..3404
                     /vntifkey="21"
                     /label=A>T
BASE COUNT     2369 a      2384 c      2512 g      2006 t 
ORIGIN
        1 atggacaaga agtactccat tgggctcgat atcggcacaa acagcgtcgg ctgggccgtc 
       61 attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc 
      121 cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa 
      181 gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc 
      241 tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg 
      301 ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc 
      361 aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag 
      421 aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat 
      481 atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat 
      541 gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg 
      601 atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg 
      661 cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat 
      721 cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa 
      781 gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc 
      841 cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt 
      901 ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt 
      961 atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga 
     1021 cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc 
     1081 ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg 
     1141 gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc 
     1201 aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac 
     1261 gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt 
     1321 gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc 
     1381 agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa 
     1441 gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa 
     1501 aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt 
     1561 tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg 
     1621 tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc 
     1681 gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc 
     1741 agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc 
     1801 attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc 
     1861 ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct 
     1921 catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg 
     1981 cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg 
     2041 gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac 
     2101 tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt 
     2161 cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc 
     2221 gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt 
     2281 atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg 
     2341 atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca 
     2401 gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg 
     2461 gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggatcat 
     2521 atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc 
     2581 gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa 
     2641 aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg 
     2701 actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag 
     2761 cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac 
     2821 accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct 
     2881 aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat 
     2941 taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa 
     3001 tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa 
     3061 atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc 
     3121 aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga 
     3181 ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc 
     3241 gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta 
     3301 cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc 
     3361 gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgtttctcc tacagtcgct 
     3421 tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc 
     3481 aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac 
     3541 tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag 
     3601 tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gcgcgagctg 
     3661 cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc 
     3721 cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa 
     3781 caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg 
     3841 atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag 
     3901 cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg 
     3961 cctgcagcct tcaagtactt cgacaccacc atagacagaa aggagtaccg ctctacaaag 
     4021 gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc 
     4081 gacctctctc agctcggtgg agacagcagg gctgacccca agaagaagag gaaggtgagg 
     4141 tccggcggcg gagagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 
     4201 ggcccaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 
     4261 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 
     4321 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 
     4381 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac 
     4441 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 
     4501 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 
     4561 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 
     4621 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 
     4681 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 
     4741 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 
     4801 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 
     4861 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 
     4921 aagtaactgc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 
     4981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     5041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 
     5101 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 
     5161 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 
     5221 tagggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 
     5281 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 
     5341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
     5401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
     5461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     5521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     5581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     5641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     5701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
     5761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     5821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     5881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     5941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 
     6001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     6061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     6121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     6181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     6241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     6301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     6361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     6421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     6481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     6541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     6601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     6661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     6721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     6781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     6841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     6901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     6961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     7021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     7081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     7141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     7201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     7261 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc 
     7321 gatcccctag ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat 
     7381 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca 
     7441 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg 
     7501 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa 
     7561 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa 
     7621 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata 
     7681 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggac 
     7741 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc 
     7801 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta 
     7861 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catgggtcga 
     7921 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt 
     7981 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggcgcg 
     8041 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg 
     8101 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc 
     8161 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg 
     8221 ccccgctccg cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac 
     8281 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac 
     8341 ggctcgtttc ttttctgtgg ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt 
     8401 gcggggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc 
     8461 ggcccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc 
     8521 gcgtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg gggctgcgag 
     8581 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg 
     8641 gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc acggcccggc 
     8701 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg 
     8761 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga 
     8821 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg 
     8881 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga 
     8941 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg 
     9001 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct 
     9061 tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg ggggacgggg 
     9121 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agtgcctctg ctaaccatgt 
     9181 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca 
     9241 tcattttggc aaagaattcg cggccgccac c 
//
