LOCUS       Bxbi_GFP_BAC_Rep        8040 bp    DNA     circular UNA 04-JUN-2015
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...3d-4qxbpd8
VERSION     urn.local...3d-4qxbpd8
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     complement(564..608)
                     /label="71.7"
     primer_bind     585..608
                     /label="pBAC-step2-transf-bp9125-9148f (D1276)"
     misc_feature    670..>675
                     /note="Geneious type: Verified by sequencing"
                     /label="12/21/11"
     misc_feature    672..675
                     /note="Geneious type: ligation"
                     /label="Ligation"
     primer_bind     672..701
                     /label="54.7"
     primer_bind     746..770
                     /label="63.2"
     promoter        752..895
                     /label="proD"
     misc_feature    895^896
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    896..902
                     /note="Geneious type: Editing History Replacement"
                     /label="CTCG"
     misc_feature    904..952
                     /note="Geneious type: Recombinase Site"
                     /label="BxbiB"
     misc_feature    953..958
                     /note="Geneious type: Editing History Insertion"
                     /label
     primer_bind     953..970
                     /label="57.3"
     terminator      complement(959..1063)
                     /label="TermT1"
     misc_feature    1063^1064
                     /note="Geneious type: Editing History Deletion"
                     /label="T"
     CDS             complement(1118..1834)
                     /label="gfp"
     misc_feature    1127
                     /note="Geneious type: Editing History Replacement"
                     /label="T"
     misc_feature    1130
                     /note="Geneious type: Editing History Replacement"
                     /label="T"
     primer_bind     complement(1807..1846)
                     /label="61.4"
     primer_bind     complement(1807..1834)
                     /label="55.1"
     misc_feature    1831
                     /note="Geneious type: Editing History Replacement"
                     /label="G"
     RBS             complement(1835..1860)
                     /label="RBS"
     primer_bind     1847..1891
                     /label="60.7"
     misc_feature    complement(1861..1935)
                     /note="Geneious type: Ribozyme"
                     /label="RiboJ"
     mRNA            complement(1861..1928)
                     /label="mRNA"
     primer_bind     1861..1891
                     /label="55.1"
     misc_feature    complement(1942..1999)
                     /note="Geneious type: Recombinase Site"
                     /label="BxbiP"
     primer_bind     complement(1942..1999)
                     /label="(BxbiP) rev, 72.9"
     primer_bind     complement(1978..1999)
                     /label
     misc_feature    2000
                     /note="Geneious type: Editing History Replacement"
                     /label="GC"
     misc_feature    2001..2004
                     /note="Geneious type: ligation"
                     /label="Ligation"
     primer_bind     2009..2038
                     /label="62.1"
     primer_bind     2009..2034
                     /label=61
     primer_bind     2010..2069
                     /label="3' (BxbiP) fwd, 69.8"
     misc_feature    complement(2020..2537)
                     /label="sopC/parC"
     misc_feature    complement(2061..2084)
                     /note="Geneious type: primer_bind_reverse"
                     /label="pBAC-step2-transf-bp2584-2561r  (D1277)"
     misc_feature    complement(2610..3581)
                     /label="sopB/parB"
     misc_feature    complement(3582..4747)
                     /label="sopA/parA"
     misc_feature    complement(5082..5332)
                     /gene="incC"
                     /note="incC; incompatibility region of the bacterial F
                     plasmid"
                     /label="incC"
     misc_feature    complement(5335..6090)
                     /label="BAC/F/repE"
     rep_origin      complement(6181..6400)
                     /note="also known as oriS; ori2; secondary origin of
                     replication for the bacterial F  plasmid"
                     /label="ori2 rep origin"
     rep_origin      6476..7090
                     /note="oriV; origin of replication for the bacterial F
                     plasmid"
                     /label="oriV rep origin"
     misc_feature    7925..680
                     /note="Geneious type: antibiotic resistance"
                     /label="CmR"
ORIGIN      
        1 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
       61 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      121 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      181 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
      241 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      301 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      361 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      421 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
      481 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
      541 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
      601 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
      661 cggggcgtaa gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
      721 tatcacgagg ccctttcgtc ttcacctcga gcacagctaa caccacgtcg tccctatctg
      781 ctgccctagg tctatgagtg gttgctggat aactttacgg gcatgcataa ggctcgtata
      841 atatattcag ggagaccaca acggtttccc tctacaaata attttgttta actttttaat
      901 taacggccgg cttgtcgacg acggcggtct ccgtcgtcag gatcatccgg gcatcgattc
      961 tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     1021 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctctagca cgcgtaccat
     1081 gggatccccc gggctgcagg aattcgatat caagctttta tttgtagaga tcatccatgc
     1141 catgtgtaat cccagcagct gttacaaact caagaaggac catgtggtct ctcttttcgt
     1201 tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt aaaaggacag
     1261 ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg cttccatctt
     1321 caatgttgtg tctaattttg aagttaactt tgattccatt cttttgtttg tctgccatga
     1381 tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg tttccatctt
     1441 ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct tcaaacttga
     1501 cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat
     1561 aaccttcggg catggcactc ttgaaaaagt catgctgttt catatgatct gggtatctcg
     1621 caaagcattg aacaccataa ccgaaagtag tgacaagtgt tggccatgga acaggtagtt
     1681 ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct
     1741 ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga
     1801 caactccagt gaaaagttct tctcctttac tcatcttaaa cctccttacc tcgtaaacta
     1861 ttaaacaaaa ttatttgtag aggctgtttc gtcctcacgg actcatcaga ccggaaagca
     1921 catccggtga cagctgtgca cgtcggggtt tgtaccgtac accactgaga ccgcggtggt
     1981 tgaccagaca aaccacgaca catgtcaata cttgcccttg acaggcattg atggaatcgt
     2041 agtctcacgc tgatagtctg atcgacaata caagtgggac cgtggtccca gaccgataat
     2101 cagaccgaca acacgagtgg gatcgtggtc ccagactaat aatcagaccg acgatacgag
     2161 tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc gtggttccag
     2221 actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga
     2281 cgatacgagt gggaccatgg tcccagacta ataatcagac cgacgatacg agtgggaccg
     2341 tggtcccagt ctgattatca gaccgacgat acgagtggga ccgtggtccc agactaataa
     2401 tcagaccgac gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga
     2461 gtgggaccgt ggtcccagtc tgattatcag accgacgata caagtggaac agtgggccca
     2521 gagagaatat tcaggccagt tatgctttct ggcctgtaac aaaggacatt aagtaaagac
     2581 agataaacgt agactaaaac gtggtcgcat cagggtgctg gcttttcaag ttccttaaga
     2641 atggcctcaa ttttctctat acactcagtt ggaacacggg acctgtccag gttaagcacc
     2701 attttatcgc ccttatacaa tactgtcgct ccaggagcaa actgatgtcg tgagcttaaa
     2761 ctagttcttg atgcagatga cgttttaagc acagaagtta aaagagtgat aacttcttca
     2821 gcttcaaata tcaccccagc ttttttctgc tcatgaaggt tagatgcctg ctgcttaagt
     2881 aattcctctt tatctgtaaa ggctttttga agtgcatcac ctgaccgggc agatagttca
     2941 ccggggtgag aaaaaagagc aacaactgat ttaggcaatt tggcggtgtt gatacagcgg
     3001 gtaataatct tacgtgaaat attttccgca tcagccagcg cagaaatatt tccagcaaat
     3061 tcattctgca atcggcttgc ataacgctga ccacgttcat aagcacttgt tgggcgataa
     3121 tcgttaccca atctggataa tgcagccatc tgctcatcat ccagctcgcc aaccagaaca
     3181 cgataatcac tttcggtaag tgcagcagct ttacgacggc gactcccatc ggcaatttct
     3241 atgacaccag atactcttcg accgaacgcc ggtgtctgtt gaccagtcag tagaaaagaa
     3301 gggatgagat catccagtgc gtcctcagta agcagctcct ggtcacgttc attacctgac
     3361 catacccgag aggtcttctc aacactatca ccccggagca cttcaagagt aaacttcaca
     3421 tcccgaccac atacaggcaa agtaatggca ttaccgcgag ccattactcc tacgcgcgca
     3481 attaacgaat ccaccatcgg ggcagctggt gtcgataacg aagtatcttc aaccggttga
     3541 gtattgagcg tatgttttgg aataacaggc gcacgcttca ttatctaatc tcccagcgtg
     3601 gtttaatcag acgatcgaaa atttcattgc agacaggttc ccaaatagaa agagcatttc
     3661 tccaggcacc agttgaagag cgttgatcaa tggcctgttc aaaaacagtt ctcatccgga
     3721 tctgaccttt accaacttca tccgtttcac gtacaacatt ttttagaacc atgcttcccc
     3781 aggcatcccg aatttgctcc tccatccacg gggactgaga gccattacta ttgctgtatt
     3841 tggtaagcaa aatacgtaca tcaggctcga accctttaag atcaacgttc ttgagcagat
     3901 cacgaagcat atcgaaaaac tgcagtgcgg aggtgtagtc aaacaactca gcaggcgtgg
     3961 gaacaatcag cacatcagca gcacatacga cattaatcgt gccgataccc aggttaggcg
     4021 cgctgtcaat aactatgaca tcatagtcat gagcaacagt ttcaatggcc agtcggagca
     4081 tcaggtgtgg atcggtgggc agtttacctt catcaaattt gcccattaac tcagtttcaa
     4141 tacggtgcag agccagacag gaaggaataa tgtcaagccc cggccagcaa gtgggcttta
     4201 ttgcataagt gacatcgtcc ttttccccaa gatagaaagg caggagagtg tcttctgcat
     4261 gaatatgaag atctggtacc catccgtgat acattgaggc tgttccctgg gggtcgttac
     4321 cttccacgag caaaacacgt agccccttca gagccagatc ctgagcaaga tgaacagaaa
     4381 ctgaggtttt gtaaacgcca cctttatggg cagcaacccc gatcaccggt ggaaatacgt
     4441 cttcagcacg tcgcaatcgc gtaccaaaca catcacgcat atgattaatt tgttcaattg
     4501 tataaccaac acgttgctca acccgtcctc gaatttccat atccgggtgc ggtagtcgcc
     4561 ctgctttctc ggcatctctg atagcctgag aagaaacccc aactaaatcc gctgcttcac
     4621 ctattctcca gcgccgggtt attttcctcg cttccgggct gtcatcatta aactgtgcaa
     4681 tggcgatagc cttcgtcatt tcatgaccag cgtttatgca ctggttaagt gtttccatga
     4741 gtttcattct gaacatcctt taatcattgc tttgcgtttt tttattaaat cttgcaattt
     4801 actgcaaagc aacaacaaaa tcgcaaagtc atcaaaaaac cgcaaagttg tttaaaataa
     4861 gagcaacact acaaaaggag ataagaagag cacatacctc agtcacttat tatcactagc
     4921 gctcgccgca gccgtgtaac cgagcatagc gagcgaactg gcgaggaagc aaagaagaac
     4981 tgttctgtca gatagctctt acgctcagcg caagaagaaa tatccaccgt gggaaaaact
     5041 ccaggtagag gtacacacgc ggatagccaa ttcagagtaa taaactgtga taatcaaccc
     5101 tcatcaatga tgacgaacta acccccgata tcaggtcaca tgacgaaggg aaagagaagg
     5161 aaatcaactg tgacaaactg ccctcaaatt tggcttcctt aaaaattaca gttcaaaaag
     5221 tatgagaaaa tccatgcagg ctgaaggaaa cagcaaaact gtgacaaatt accctcagta
     5281 ggtcagaaca aatgtgacga accaccctca aatctgtgac agataaccct cagactatcc
     5341 tgtcgtcatg gaagtgatat cgcggaagga aaatacgata tgagtcgtct ggcggccttt
     5401 ctttttctca atgtatgaga ggcgcattgg agttctgctg ttgatctcat taacacagac
     5461 ctgcaggaag cggcggcgga agtcaggcat acgctggtaa ctttgaggca gctggtaacg
     5521 ctctatgatc cagtcgattt tcagagagac gatgcctgag ccatccggct tacgatactg
     5581 acacagggat tcgtataaac gcatggcata cggattggtg atttcttttg tttcactaag
     5641 ccgaaactgc gtaaaccggt tctgtaaccc gataaagaag ggaatgagat atgggttgat
     5701 atgtacactg taaagccctc tggatggact gtgcgcacgt ttgataaacc aaggaaaaga
     5761 ttcatagcct ttttcatcgc cggcatcctc ttcagggcga taaaaaacca cttccttccc
     5821 cgcgaaactc ttcaatgcct gccgtatatc cttactggct tccgcagagg tcaatccgaa
     5881 tatttcagca tatttagcaa catggatctc gcagataccg tcatgttcct gtagggtgcc
     5941 atcagatttt ctgatctggt caacgaacag atacagcata cgtttttgat cccgggagag
     6001 actatatgcc gcctcagtga ggtcgtttga ctggacgatt cgcgggctat ttttacgttt
     6061 cttgtgattg ataaccgctg tttccgccat gacagatcca tgtgaagtgt gacaagtttt
     6121 tagattgtca cactaaataa aaaagagtca ataagcaggg ataactttgt gaaaaaacag
     6181 cttcttctga gggcaatttg tcacagggtt aagggcaatt tgtcacagac aggactgtca
     6241 tttgagggtg atttgtcaca ctgaaagggc aatttgtcac aacaccttct ctagaaccag
     6301 catggataaa ggcctacaag gcgctctaaa aaagaagatc taaaaactat aaaaaaaata
     6361 attataaaaa tatccccgtg gataagtgga taaccccaag ggaagttttt tcaggcatcg
     6421 tgtgtaagca gaatatataa gtgctgttcc ctggtgcttc ctcgctcact cgaccgggag
     6481 ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc
     6541 ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg
     6601 gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc
     6661 ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga
     6721 tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat
     6781 ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat
     6841 ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa
     6901 cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag
     6961 ggccaagttt tccgcgaggt atccacaacg ccggcggccg gccgcggtgt ctcgcacacg
     7021 gcttcgacgg cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga
     7081 gggcaaccag ccgagggctt cgccctgtcg ctcgactgcg gcgagcacta ctggctgtaa
     7141 aaggacagac cacatcatgg ttctgtgttc attaggttgt tctgtccatt gctgacataa
     7201 tccgctccac ttcaacgtaa caccgcacga agatttctat tgttcctgaa ggcatattca
     7261 aatcgttttc gttaccgctt gcaggcatca tgacagaaca ctacttccta taaacgctac
     7321 acaggctcct gagattaata atgcggatct ctacgataat gggagatttt cccgactgtt
     7381 tcgttcgctt ctcagtggat aacagccagc ttctctgttt aacagacaaa aacagcatat
     7441 ccactcagtt ccacatttcc atataaaggc caaggcattt attctcagga taattgtttc
     7501 agcatcgcaa ccgcatcaga ctccggcatc gcaaactgca cccggtgccg ggcagccaca
     7561 tccagcgcaa aaaccttcgt gtagacttcc gttgaactga tggacttatg tcccatcagg
     7621 ctttgcagaa ctttcagcgg tataccggca tacagcatgt gcatcgcata ggaatggcgg
     7681 aacgtatgtg gtgtgaccgg aacagagaac gtcacaccgt cagcagcagc ggcggcaacc
     7741 gcctccccaa tccaggtcct gaccgttctg tccgtcactt cccagatccg cgctttctct
     7801 gtccttcctg tgcgacggtt acgccgctcc atgagcttat cgcgaataaa tacctgtgac
     7861 ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg
     7921 ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca
     7981 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
//
