LOCUS       pZS2katGp-RBS33-        6544 bp ds-DNA     circular     04-JUN-2015
DEFINITION  .
ACCESSION   urn.local...c-4qx8xg7
VERSION
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     katGp-RBS0033-TP901-Term1-ProD-OxyR-pSC101-KanR.
FEATURES             Location/Qualifiers
     gene            1..1455
                     /note="Geneious type: CDS"
                     /label="tp901"
     misc_feature    1..3
                     /label
     primer_bind     4..33
                     /label=55
     primer_bind     4..21
                     /label="primer bind on Nate's plasmid"
     primer_bind     236..258
                     /label="NR111"
     misc_feature    1163..1662
                     /label="gblock"
     primer_bind     complement(1421..1455)
                     /label="66.6"
     primer_bind     complement(1421..1455)
                     /label="55.3"
     misc_feature    1421..1455
                     /label="Ligation"
     primer_bind     1456..1485
                     /label="74.6"
     primer_bind     1456..1485
                     /label="66.7"
     CDS             1462..1497
                     /label="AAV+Stop"
     misc_feature    1552..1557
                     /label
     terminator      1558..1662
                     /label="TermT1"
     misc_feature    1664..1667
                     /label="Ligation"
     misc_feature    1664..1667
                     /label
     misc_feature    1667..1668
                     /label="CCTAG"
     misc_feature    1667..1668
                     /label="GA"
     promoter        1668..1811
                     /label="proD"
     RBS             1818..1832
                     /label="RBS29"
     CDS             1839..2756
                     /label="oxyR"
     misc_feature    2756..2757
                     /label="A"
     misc_feature    2763..2768
                     /label="BamHI"
     misc_feature    2775..2780
                     /label="MluI"
     terminator      2787..2891
                     /label="TermT1"
     rep_origin      2905..5125
                     /label="pSC101"
     misc_feature    complement(2905..5125)
                     /label="pSC101"
     misc_feature    complement(3040..3044)
                     /label="HgaI, CseI"
     misc_feature    3091..3101
                     /label="EcoNI, BstENI, XagI"
     misc_feature    3318..3323
                     /label="NdeI, FauNDI"
     misc_feature    3339..3344
                     /label="EcoRV, Eco32I"
     misc_feature    3415..3427
                     /label="AloI"
     misc_feature    3599..3604
                     /label="SnaBI, BstSNI, Eco105I"
     misc_feature    3970..3975
                     /label="PciI, PscI"
     misc_feature    4437..4442
                     /label="SpeI, AhlI, BcuI"
     misc_feature    complement(4694..4699)
                     /label="BseRI"
     misc_feature    4728..4733
                     /label="AflII, BfrI, BspTI, Bst98I, BstAFI, MspCI,Vha464I"
     misc_feature    4765..4770
                     /label="SspI"
     misc_feature    4817..4829
                     /label="ArsI"
     misc_feature    4997..5005
                     /label="AlwNI, CaiI, PstNI"
     terminator      complement(5126..5231)
                     /label="TermT0"
     misc_feature    5232..5237
                     /label="SacI, Psp124BI, SstI"
     misc_feature    5232..5237
                     /label="Ecl136II, Eco53kI, EcoICRI"
     misc_feature    complement(5238..6187)
                     /note="Geneious type: Antibiotic Resistance"
                     /label="KanR"
     misc_feature    complement(5238..6187)
                     /label="KanR"
     misc_feature    5248..5258
                     /label="BplI"
     misc_feature    5402..5408
                     /label="RsrII, CpoI, CspI, Rsr2I"
     misc_feature    5403..5407
                     /label="AvaII, Bme18I, Eco47I, SinI, VpaK11BI"
     misc_feature    5521..5526
                     /label="BsePI, BssHII, PauI, PteI"
     misc_feature    5533..5537
                     /label="EcoRII, AjnI, Psp6I, PspGI"
     misc_feature    5533..5537
                     /label="BseBI, Bst2UI, BstNI, BstOI, MvaI"
     misc_feature    5596..5606
                     /label="FalI"
     misc_feature    complement(5692..5697)
                     /label="BsrDI, Bse3DI, BseMI"
     misc_feature    complement(5719..5724)
                     /label="NmeAIII"
     misc_feature    5800..5808
                     /label="Tth111I, AspI, PflFI, PsyI"
     misc_feature    5818..5823
                     /label="Acc16I, AviII, FspI, NsbI"
     misc_feature    5838..5843
                     /label="BalI, MlsI, MluNI, MscI, Msp20I"
     misc_feature    5869..5874
                     /label="PstI"
     misc_feature    5891..5902
                     /label="DrdI, AasI, DseDI"
     misc_feature    6012..6017
                     /label="BseX3I, BstZI, EagI, EclXI, Eco52I"
     misc_feature    6077..6082
                     /label="BclI, FbaI, Ksp22I"
     misc_feature    6082..6087
                     /label="BglII"
     misc_feature    6100..6114
                     /label="XcmI"
     misc_feature    6162..6193
                     /label=65
     misc_feature    6188..6193
                     /label="AatII"
     misc_feature    6188..6193
                     /label="ZraI"
     CDS             6194..6520
                     /note="Geneious type: promoter"
                     /label="katGp"
     RBS             6528..6538
                     /label="RBS33"
     misc_feature    6528..6538
                     /label="RBS 0033"
ORIGIN      
        1 atgactaaga aagtagcaat ctatacacga gtatccacta ctaaccaagc agaggaaggc
       61 ttctcaattg atgagcaaat tgaccgttta acaaaatatg ctgaagcaat ggggtggcaa
      121 gtatctgata cttatactga tgctggtttt tcaggggcca aacttgaacg cccagcaatg
      181 caaagattaa tcaacgatat cgagaataaa gcttttgata cagttcttgt atataagcta
      241 gaccgccttt cacgtagtgt aagagatact ctttatcttg ttaaggatgt gttcacaaaa
      301 aataaaatag actttatctc gcttaatgaa agtattgata cttcttctgc tatgggtagc
      361 ttgtttctca ctattctttc tgcaattaat gagtttgaaa gagagaatat aaaagaacgc
      421 atgactatgg gtaaactagg gcgagcgaaa tctggtaagt ctatgatgtg gactaagaca
      481 gcttttgggt attaccacaa cagaaagaca ggtatattag aaattgttcc tttacaagct
      541 acaatagttg aacaaatatt cactgattat ttatcaggaa tatcacttac aaaattaaga
      601 gataaactca atgaatctgg acacatcggt aaagatatac cgtggtctta tcgtacccta
      661 agacaaacac ttgataatcc agtttactgt ggttatatca aatttaagga cagcctattt
      721 gaaggtatgc acaaaccaat tatcccttat gagacttatt taaaagttca aaaagagcta
      781 gaagaaagac aacagcagac ttatgaaaga aataacaacc ctagaccttt ccaagctaaa
      841 tatatgctgt cagggatggc aaggtgcggt tactgtggag cacctttaaa aattgttctt
      901 ggccacaaaa gaaaagatgg aagccgcact atgaaatatc actgtgcaaa tagatttcct
      961 cgaaaaacaa aaggaattac agtatataat gacaataaaa agtgtgattc aggaacttat
     1021 gatttaagta atttagaaaa tactgttatt gacaacctga ttggatttca agaaaataat
     1081 gactccttat tgaaaattat caatggcaac aaccaaccta ttcttgatac ttcgtcattt
     1141 aaaaagcaaa tttcacagat cgataaaaaa atacaaaaga actctgattt gtacctaaat
     1201 gattttatca ctatggatga gttgaaagat cgtactgatt cccttcaggc tgagaaaaag
     1261 ctgcttaaag ctaagattag cgaaaataaa tttaatgact ctactgatgt ttttgagtta
     1321 gttaaaactc agttgggctc aattccgatt aatgaactat catatgataa taaaaagaaa
     1381 atcgtcaaca accttgtatc aaaggttgat gttactgctg ataatgtaga tatcatattt
     1441 aaattccaac tcgctaggcc tgcagcaaac gacgaaaact acgctgcagc agtttagaca
     1501 catggcatgg atgaactata caaataaccc gggggatccc atggtacgcg tgctagaggc
     1561 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1621 cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacctagcac agctaacacc
     1681 acgtcgtccc tatctgctgc cctaggtcta tgagtggttg ctggataact ttacgggcat
     1741 gcataaggct cgtataatat attcagggag accacaacgg tttccctcta caaataattt
     1801 tgtttaactt tgaattcttc acacaggaaa ccggtaccat gaatattcgt gatcttgagt
     1861 acctggtggc attggctgaa caccgccatt ttcggcgtgc ggcagattcc tgccacgtta
     1921 gccagccgac gcttagcggg caaattcgta agctggaaga tgagctgggc gtgatgttgc
     1981 tggagcggac cagccgtaaa gtgttgttca cccaggcggg aatgctgctg gtggatcagg
     2041 cgcgtaccgt gctgcgtgag gtgaaagtcc ttaaagagat ggcaagccag cagggcgaga
     2101 cgatgtccgg accgctgcac attggtttga ttcccacagt tggaccgtac ctgctaccgc
     2161 atattatccc tatgctgcac cagacctttc caaagctgga aatgtatctg catgaagcac
     2221 agacccacca gttactggcg caactggaca gcggcaaact cgattgcgtg atcctcgcgc
     2281 tggtgaaaga gagcgaagca ttcattgaag tgccgttgtt tgatgagcca atgttgctgg
     2341 ctatctatga agatcacccg tgggcgaacc gcgaatgcgt accgatggcc gatctggcag
     2401 gggaaaaact gctgatgctg gaagatggtc actgtttgcg cgatcaggca atgggtttct
     2461 gttttgaagc cggggcggat gaagatacac acttccgcgc gaccagcctg gaaactctgc
     2521 gcaacatggt ggcggcaggt agcgggatca ctttactgcc agcgctggct gtgccgccgg
     2581 agcgcaaacg cgatggggtt gtttatctgc cgtgcattaa gccggaacca cgccgcacta
     2641 ttggcctggt ttatcgtcct ggctcaccgc tgcgcagccg ctatgagcag ctggcagagg
     2701 ccatccgcgc aagaatggat ggccatttcg ataaagtttt aaaacaggcg gtttaacccg
     2761 ggggatccca tggtacgcgt gctagaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2821 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     2881 gccgccctag acctagggcc tagggtacgg gttttgctgc ccgcaaacgg gctgttctgg
     2941 tgttgctagt ttgttatcag aatcgcagat ccggcttcag gtttgccggc tgaaagcgct
     3001 atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg
     3061 tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt
     3121 gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg
     3181 tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat
     3241 ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta
     3301 caccgttttc atctgtgcat atggacagtt ttccctttga tatctaacgg tgaacagttg
     3361 ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct
     3421 cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc
     3481 catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa ttttgcctca
     3541 aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta
     3601 cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg
     3661 ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac
     3721 gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa
     3781 tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt
     3841 tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt
     3901 ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca
     3961 aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa aagataaggc
     4021 aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc
     4081 cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc
     4141 agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc
     4201 tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc
     4261 gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca
     4321 tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat
     4381 tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta
     4441 gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc tggaaaactt
     4501 gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat
     4561 attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat
     4621 cccagccctg tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg
     4681 caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc
     4741 ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag
     4801 tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg
     4861 taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga
     4921 aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg
     4981 actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat
     5041 cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag
     5101 gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     5161 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     5221 ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     5281 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     5341 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     5401 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     5461 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     5521 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     5581 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     5641 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     5701 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     5761 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     5821 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     5881 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     5941 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     6001 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     6061 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     6121 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     6181 caattccgac gtctgtggct tttatgaaaa tcacacagtg atcacaaatt ttaaacagag
     6241 cacaaaatgc tgcctcgaaa tgagggcggg aaaataaggt tatcagcctt gttttctccc
     6301 tcattacttg aaggatatga agctaaaacc cttttttata aagcatttgt ccgaattcgg
     6361 acataatcaa aaaagcttaa ttaagatcaa tttgatctac atctctttaa ccaacaatat
     6421 gtaagatctc aactatcgca tccgtggatt aattcaatta taacttctct ctaacgctgt
     6481 gtatcgtaac ggtaacactg tagaggggag cacattgatg cgaattctca cacaggacgg
     6541 tacc
//
