LOCUS       TP901_GFP_BAC_Re        8036 bp    DNA     circular UNA 04-JUN-2015
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...5a-4qxcowz
VERSION     urn.local...5a-4qxcowz
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     complement(564..608)
                     /label="71.7"
     misc_feature    584..>956
                     /note="Geneious type: try to seq"
                     /label=185
     primer_bind     585..608
                     /label="pBAC-step2-transf-bp9125-9148f (D1276)"
     misc_feature    666..1616
                     /note="Geneious type: Verified by sequencing"
                     /label="A1"
     misc_feature    670..>675
                     /note="Geneious type: Verified by sequencing"
                     /label="12/21/11"
     misc_feature    672..675
                     /note="Geneious type: ligation"
                     /label="Ligation"
     primer_bind     672..701
                     /label="54.7"
     primer_bind     746..770
                     /label="63.2"
     promoter        752..895
                     /label="proD"
     primer_bind     complement(859..948)
                     /label=68
     primer_bind     complement(859..903)
                     /label="58.9"
     misc_feature    895^896
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    904..956
                     /note="Geneious type: Recombinase Site"
                     /label="TP901B"
     primer_bind     957..974
                     /label="57.3"
     terminator      complement(963..1067)
                     /label="TermT1"
     primer_bind     complement(1044..1067)
                     /label="TermT1 5' Forward"
     misc_feature    1068..1863
                     /note="Geneious type: Editing History Replacement"
                     /label="ACGCGTACCATGGGATCCCCCGGGTTATTTGTACAATTCATCCATACCAT
                     GGGTAATACCAGCAGCAGTCCTAAATTCTAACAGGACCATGTGGTCTCTCTTTTCGTT
                     TGGATCTTTGGATAAGGCTGATTGGGTGGATAAGTAATGGTTGTCTGGTAACAAGACT
                     GGACCATCACCAATTGGAGTATTTTGTTGATAATGGTCAGCTAATTGAACAGAACCAT
                     CTTCAATGTTGTGTCTAATTTTGAAGTTCACTTTGATACCATTCTTTTGTTTGTCAGC
                     CATGATGTATATATTGTGAGAGTTGAAGTTGTATTCCAATTTGTGACCTAAAATGTTA
                     CCATCTTCTTTAAAATCAATACCTTTTAATTCGATTCTATTAACTAAGGTATCACCTT
                     CAAACTTGACTTCAGCTCTGGTCTTGTAGTTACCGTCATCTTTGAAAAAAATAGTTCT
                     TTCTTGAACATAACCTTCTGGCATGGCAGACTTGAAAAAGTCATGTTGTTTCATATGA
                     TCTGGGTATCTAGAAAAACATTGAACACCATGGCTCAAAGTAGTTACTAAGGTTGGCC
                     ATGGAACTGGCAATTTACCAGTAGTACAAATAAATTTTAAGGTCAATTTACCGTACGT
                     AGCATCACCTTCACCTTCACCGGAGACAGAAAATTTGTGACCATTAACATCACCATCT
                     AATTCAACCAAAATTGGGACAACACCAGTGAATAATTCTTCACCTTTAGACATTTTTA
                     ACCTCCTCCCTACGTACTC"
     CDS             complement(1122..1838)
                     /label="gfp"
     misc_feature    1278..2252
                     /note="Geneious type: Verified by sequencing"
                     /label="A1"
     primer_bind     complement(1811..1850)
                     /label="61.4"
     primer_bind     complement(1811..1838)
                     /label="55.1"
     RBS             complement(1839..1864)
                     /label="RBS"
     primer_bind     1851..1895
                     /label="60.7"
     misc_feature    complement(1865..1939)
                     /note="Geneious type: Ribozyme"
                     /label="RiboJ"
     mRNA            complement(1865..1932)
                     /label="mRNA"
     primer_bind     1865..1895
                     /label="55.1"
     misc_feature    complement(1946..1995)
                     /note="Geneious type: Recombinase Site"
                     /label="TP901P"
     misc_feature    <1946..2033
                     /note="Geneious type: try to seq"
                     /label=104
     misc_feature    <1946..2080
                     /note="Geneious type: Verified by sequencing"
                     /label="F2"
     primer_bind     complement(<1946..1995)
                     /label="67.7"
     primer_bind     1996..2020
                     /label="63.4"
     primer_bind     2001..2030
                     /label="65.1"
     primer_bind     2005..2034
                     /label="62.1"
     primer_bind     2005..2030
                     /label=61
     primer_bind     2006..2065
                     /label="3' (BxbiP) fwd, 69.8"
     misc_feature    complement(2016..2533)
                     /label="sopC/parC"
     misc_feature    complement(2057..2080)
                     /note="Geneious type: primer_bind_reverse"
                     /label="pBAC-step2-transf-bp2584-2561r  (D1277)"
     misc_feature    complement(2606..3577)
                     /label="sopB/parB"
     misc_feature    complement(3578..4743)
                     /label="sopA/parA"
     misc_feature    complement(5078..5328)
                     /gene="incC"
                     /note="incC; incompatibility region of the bacterial F
                     plasmid"
                     /label="incC"
     misc_feature    complement(5331..6086)
                     /label="BAC/F/repE"
     rep_origin      complement(6177..6396)
                     /note="also known as oriS; ori2; secondary origin of
                     replication for the bacterial F  plasmid"
                     /label="ori2 rep origin"
     rep_origin      6472..7086
                     /note="oriV; origin of replication for the bacterial F
                     plasmid"
                     /label="oriV rep origin"
     misc_feature    7921..680
                     /note="Geneious type: antibiotic resistance"
                     /label="CmR"
ORIGIN      
        1 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
       61 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      121 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      181 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
      241 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      301 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      361 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      421 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
      481 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
      541 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
      601 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
      661 cggggcgtaa gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
      721 tatcacgagg ccctttcgtc ttcacctcga gcacagctaa caccacgtcg tccctatctg
      781 ctgccctagg tctatgagtg gttgctggat aactttacgg gcatgcataa ggctcgtata
      841 atatattcag ggagaccaca acggtttccc tctacaaata attttgttta actttttaat
      901 taaatgccaa cacaattaac atctcaatca aggtaaatgc tttttgcttt ttttgcatcg
      961 attctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     1021 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctct agcacgcgta
     1081 ccatgggatc ccccgggctg caggaattcg atatcaagct tttatttgta gagatcatcc
     1141 atgccatgtg taatcccagc agctgttaca aactcaagaa ggaccatgtg gtctctcttt
     1201 tcgttgggat ctttcgaaag ggcagattgt gtggacaggt aatggttgtc tggtaaaagg
     1261 acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg aacgcttcca
     1321 tcttcaatgt tgtgtctaat tttgaagtta actttgattc cattcttttg tttgtctgcc
     1381 atgatgtata cattgtgtga gttatagttg tattccaatt tgtgtccaag aatgtttcca
     1441 tcttctttaa aatcaatacc ttttaactcg attctattaa caagggtatc accttcaaac
     1501 ttgacttcag cacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt
     1561 acataacctt cgggcatggc actcttgaaa aagtcatgct gtttcatatg atctgggtat
     1621 ctcgcaaagc attgaacacc ataaccgaaa gtagtgacaa gtgttggcca tggaacaggt
     1681 agttttccag tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca
     1741 ccctctccac tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt
     1801 gggacaactc cagtgaaaag ttcttctcct ttactcatct taaacctcct tacctcgtaa
     1861 actattaaac aaaattattt gtagaggctg tttcgtcctc acggactcat cagaccggaa
     1921 agcacatccg gtgacagctg tgtttaaagg agttttttag ttaccttaat tgaaataaac
     1981 gaaataaaaa ctcgccgagc accgtacttg cccttgacag gcattgatgg aatcgtagtc
     2041 tcacgctgat agtctgatcg acaatacaag tgggaccgtg gtcccagacc gataatcaga
     2101 ccgacaacac gagtgggatc gtggtcccag actaataatc agaccgacga tacgagtggg
     2161 accgtggtcc cagactaata atcagaccga cgatacgagt gggaccgtgg ttccagacta
     2221 ataatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat
     2281 acgagtggga ccatggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt
     2341 cccagtctga ttatcagacc gacgatacga gtgggaccgt ggtcccagac taataatcag
     2401 accgacgata cgagtgggac cgtggtccca gactaataat cagaccgacg atacgagtgg
     2461 gaccgtggtc ccagtctgat tatcagaccg acgatacaag tggaacagtg ggcccagaga
     2521 gaatattcag gccagttatg ctttctggcc tgtaacaaag gacattaagt aaagacagat
     2581 aaacgtagac taaaacgtgg tcgcatcagg gtgctggctt ttcaagttcc ttaagaatgg
     2641 cctcaatttt ctctatacac tcagttggaa cacgggacct gtccaggtta agcaccattt
     2701 tatcgccctt atacaatact gtcgctccag gagcaaactg atgtcgtgag cttaaactag
     2761 ttcttgatgc agatgacgtt ttaagcacag aagttaaaag agtgataact tcttcagctt
     2821 caaatatcac cccagctttt ttctgctcat gaaggttaga tgcctgctgc ttaagtaatt
     2881 cctctttatc tgtaaaggct ttttgaagtg catcacctga ccgggcagat agttcaccgg
     2941 ggtgagaaaa aagagcaaca actgatttag gcaatttggc ggtgttgata cagcgggtaa
     3001 taatcttacg tgaaatattt tccgcatcag ccagcgcaga aatatttcca gcaaattcat
     3061 tctgcaatcg gcttgcataa cgctgaccac gttcataagc acttgttggg cgataatcgt
     3121 tacccaatct ggataatgca gccatctgct catcatccag ctcgccaacc agaacacgat
     3181 aatcactttc ggtaagtgca gcagctttac gacggcgact cccatcggca atttctatga
     3241 caccagatac tcttcgaccg aacgccggtg tctgttgacc agtcagtaga aaagaaggga
     3301 tgagatcatc cagtgcgtcc tcagtaagca gctcctggtc acgttcatta cctgaccata
     3361 cccgagaggt cttctcaaca ctatcacccc ggagcacttc aagagtaaac ttcacatccc
     3421 gaccacatac aggcaaagta atggcattac cgcgagccat tactcctacg cgcgcaatta
     3481 acgaatccac catcggggca gctggtgtcg ataacgaagt atcttcaacc ggttgagtat
     3541 tgagcgtatg ttttggaata acaggcgcac gcttcattat ctaatctccc agcgtggttt
     3601 aatcagacga tcgaaaattt cattgcagac aggttcccaa atagaaagag catttctcca
     3661 ggcaccagtt gaagagcgtt gatcaatggc ctgttcaaaa acagttctca tccggatctg
     3721 acctttacca acttcatccg tttcacgtac aacatttttt agaaccatgc ttccccaggc
     3781 atcccgaatt tgctcctcca tccacgggga ctgagagcca ttactattgc tgtatttggt
     3841 aagcaaaata cgtacatcag gctcgaaccc tttaagatca acgttcttga gcagatcacg
     3901 aagcatatcg aaaaactgca gtgcggaggt gtagtcaaac aactcagcag gcgtgggaac
     3961 aatcagcaca tcagcagcac atacgacatt aatcgtgccg atacccaggt taggcgcgct
     4021 gtcaataact atgacatcat agtcatgagc aacagtttca atggccagtc ggagcatcag
     4081 gtgtggatcg gtgggcagtt taccttcatc aaatttgccc attaactcag tttcaatacg
     4141 gtgcagagcc agacaggaag gaataatgtc aagccccggc cagcaagtgg gctttattgc
     4201 ataagtgaca tcgtcctttt ccccaagata gaaaggcagg agagtgtctt ctgcatgaat
     4261 atgaagatct ggtacccatc cgtgatacat tgaggctgtt ccctgggggt cgttaccttc
     4321 cacgagcaaa acacgtagcc ccttcagagc cagatcctga gcaagatgaa cagaaactga
     4381 ggttttgtaa acgccacctt tatgggcagc aaccccgatc accggtggaa atacgtcttc
     4441 agcacgtcgc aatcgcgtac caaacacatc acgcatatga ttaatttgtt caattgtata
     4501 accaacacgt tgctcaaccc gtcctcgaat ttccatatcc gggtgcggta gtcgccctgc
     4561 tttctcggca tctctgatag cctgagaaga aaccccaact aaatccgctg cttcacctat
     4621 tctccagcgc cgggttattt tcctcgcttc cgggctgtca tcattaaact gtgcaatggc
     4681 gatagccttc gtcatttcat gaccagcgtt tatgcactgg ttaagtgttt ccatgagttt
     4741 cattctgaac atcctttaat cattgctttg cgttttttta ttaaatcttg caatttactg
     4801 caaagcaaca acaaaatcgc aaagtcatca aaaaaccgca aagttgttta aaataagagc
     4861 aacactacaa aaggagataa gaagagcaca tacctcagtc acttattatc actagcgctc
     4921 gccgcagccg tgtaaccgag catagcgagc gaactggcga ggaagcaaag aagaactgtt
     4981 ctgtcagata gctcttacgc tcagcgcaag aagaaatatc caccgtggga aaaactccag
     5041 gtagaggtac acacgcggat agccaattca gagtaataaa ctgtgataat caaccctcat
     5101 caatgatgac gaactaaccc ccgatatcag gtcacatgac gaagggaaag agaaggaaat
     5161 caactgtgac aaactgccct caaatttggc ttccttaaaa attacagttc aaaaagtatg
     5221 agaaaatcca tgcaggctga aggaaacagc aaaactgtga caaattaccc tcagtaggtc
     5281 agaacaaatg tgacgaacca ccctcaaatc tgtgacagat aaccctcaga ctatcctgtc
     5341 gtcatggaag tgatatcgcg gaaggaaaat acgatatgag tcgtctggcg gcctttcttt
     5401 ttctcaatgt atgagaggcg cattggagtt ctgctgttga tctcattaac acagacctgc
     5461 aggaagcggc ggcggaagtc aggcatacgc tggtaacttt gaggcagctg gtaacgctct
     5521 atgatccagt cgattttcag agagacgatg cctgagccat ccggcttacg atactgacac
     5581 agggattcgt ataaacgcat ggcatacgga ttggtgattt cttttgtttc actaagccga
     5641 aactgcgtaa accggttctg taacccgata aagaagggaa tgagatatgg gttgatatgt
     5701 acactgtaaa gccctctgga tggactgtgc gcacgtttga taaaccaagg aaaagattca
     5761 tagccttttt catcgccggc atcctcttca gggcgataaa aaaccacttc cttccccgcg
     5821 aaactcttca atgcctgccg tatatcctta ctggcttccg cagaggtcaa tccgaatatt
     5881 tcagcatatt tagcaacatg gatctcgcag ataccgtcat gttcctgtag ggtgccatca
     5941 gattttctga tctggtcaac gaacagatac agcatacgtt tttgatcccg ggagagacta
     6001 tatgccgcct cagtgaggtc gtttgactgg acgattcgcg ggctattttt acgtttcttg
     6061 tgattgataa ccgctgtttc cgccatgaca gatccatgtg aagtgtgaca agtttttaga
     6121 ttgtcacact aaataaaaaa gagtcaataa gcagggataa ctttgtgaaa aaacagcttc
     6181 ttctgagggc aatttgtcac agggttaagg gcaatttgtc acagacagga ctgtcatttg
     6241 agggtgattt gtcacactga aagggcaatt tgtcacaaca ccttctctag aaccagcatg
     6301 gataaaggcc tacaaggcgc tctaaaaaag aagatctaaa aactataaaa aaaataatta
     6361 taaaaatatc cccgtggata agtggataac cccaagggaa gttttttcag gcatcgtgtg
     6421 taagcagaat atataagtgc tgttccctgg tgcttcctcg ctcactcgac cgggagggtt
     6481 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg
     6541 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
     6601 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
     6661 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
     6721 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
     6781 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
     6841 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
     6901 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
     6961 aagttttccg cgaggtatcc acaacgccgg cggccggccg cggtgtctcg cacacggctt
     7021 cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc
     7081 aaccagccga gggcttcgcc ctgtcgctcg actgcggcga gcactactgg ctgtaaaagg
     7141 acagaccaca tcatggttct gtgttcatta ggttgttctg tccattgctg acataatccg
     7201 ctccacttca acgtaacacc gcacgaagat ttctattgtt cctgaaggca tattcaaatc
     7261 gttttcgtta ccgcttgcag gcatcatgac agaacactac ttcctataaa cgctacacag
     7321 gctcctgaga ttaataatgc ggatctctac gataatggga gattttcccg actgtttcgt
     7381 tcgcttctca gtggataaca gccagcttct ctgtttaaca gacaaaaaca gcatatccac
     7441 tcagttccac atttccatat aaaggccaag gcatttattc tcaggataat tgtttcagca
     7501 tcgcaaccgc atcagactcc ggcatcgcaa actgcacccg gtgccgggca gccacatcca
     7561 gcgcaaaaac cttcgtgtag acttccgttg aactgatgga cttatgtccc atcaggcttt
     7621 gcagaacttt cagcggtata ccggcataca gcatgtgcat cgcataggaa tggcggaacg
     7681 tatgtggtgt gaccggaaca gagaacgtca caccgtcagc agcagcggcg gcaaccgcct
     7741 ccccaatcca ggtcctgacc gttctgtccg tcacttccca gatccgcgct ttctctgtcc
     7801 ttcctgtgcg acggttacgc cgctccatga gcttatcgcg aataaatacc tgtgacggaa
     7861 gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa gccctgggcc
     7921 aacttttggc gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat
     7981 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaa
//
