LOCUS       Ternary_BAC_Repo        9944 bp    DNA     circular UNA 04-JUN-2015
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...71-4r2l49u
VERSION     urn.local...71-4r2l49u
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     complement(564..608)
                     /label="71.7"
     misc_feature    583..1432
                     /note="Geneious type: try to seq"
                     /label=185
                     585..689
     primer_bind     585..608
                     /label="pBAC-step2-transf-bp9125-9148f (D1276)"
     misc_feature    637..1177
                     /note="Geneious type: Verified by sequencing"
                     /label="E2"
     primer_bind     complement(650..689)
                     /label="69.1"
     primer_bind     650..674
                     /label="5' (termT1)-(mCherry)-(RBS)-(RiboJ) geneblock fwd,
                     69.2"
     primer_bind     complement(654..689)
                     /label=533
     primer_bind     complement(665..689)
                     /label="54.8"
     misc_feature    672..675
                     /note="Geneious type: ligation"
                     /label="Ligation"
     misc_feature    690..1645
                     /note="Geneious type: Editing History Insertion"
                     /label
     terminator      complement(690..794)
                     /label="TermT1"
     misc_feature    complement(<690..819)
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1)"
     misc_feature    complement(<690..>794)
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE11G - From Allen - Fragment 1 (concatenated sequence
                     1); Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1)"
     primer_bind     690..709
                     /label="59.7"
     primer_bind     complement(771..794)
                     /label="TermT1 5' Forward"
     CDS             complement(825..1535)
                     /label="mCherry"
     misc_feature    885..1728
                     /note="Geneious type: try to seq"
                     /label=134
     misc_feature    939..1580
                     /note="Geneious type: Verified by sequencing"
                     /label="E2"
     misc_feature    1385..1826
                     /note="Geneious type: geneblock"
                     /label="geneblock"
     primer_bind     complement(1385..1424)
                     /label="78.5"
     misc_feature    complement(1527..2342)
                     /note="Geneious type: Verified by sequencing"
                     /label="E2"
     RBS             complement(1536..1564)
                     /label="RBS"
     misc_feature    complement(1543..2395)
                     /note="Geneious type: try to seq"
                     /label=580
     misc_feature    complement(1565..1639)
                     /note="Geneious type: Ribozyme"
                     /label="RiboJ"
     mRNA            complement(1565..1632)
                     /note="Geneious type: mrna"
                     /label="mRNA"
     primer_bind     1565..1595
                     /label="55.1"
     primer_bind     complement(1621..1680)
                     /label="79.1"
     primer_bind     complement(1621..1645)
                     /label="64.7"
     misc_feature    1646..1690
                     /note="Geneious type: Recombinase Site"
                     /label="PhiC AttB"
     primer_bind     1646..1719
                     /label="642, PhiC AttB-(ECK120029600) Fwd, 76.1"
     primer_bind     1646..1675
                     /label="76.7"
     primer_bind     1695..1719
                     /label="54.3"
     terminator      complement(1697..1786)
                     /note="Geneious type: Terminator"
                     /label="ECK120029600"
     primer_bind     1787..1826
                     /label="65.7"
     misc_feature    complement(2348..3239)
                     /note="Geneious type: try to seq"
                     /label=18
     misc_feature    complement(2382..3175)
                     /note="Geneious type: Verified by sequencing"
                     /label="E2"
     primer_bind     2529..2553
                     /label="63.2"
     promoter        2535..2678
                     /note="Geneious type: Promoter"
                     /label="ProD"
     primer_bind     complement(2652..2726)
                     /label="ProD-(PhiCP) Rev, 70.2"
     primer_bind     complement(2652..2684)
                     /label="55.6"
     misc_feature    complement(2685..2726)
                     /note="Geneious type: Recombinase Site"
                     /label="PhiC AttP"
     misc_feature    complement(2707..2726)
                     /note="Geneious type: primer_bind_reverse"
                     /label=61
     primer_bind     2727..2766
                     /label="65.8"
     misc_feature    2730..2806
                     /note="Geneious type: Editing History Replacement"
                     /label="TCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTT
                     CACCTCGAGCACAGCTAACACCACGTCGTCCCTATCTGCTGCCCTAGGTCTATGAGTG
                     GTTGCTGGATAACTTTACGGGCATGCATAAGGCTCGTATAATATATTCAGGGAGACCA
                     CAACGGTTTCCCTCTACAAATAATTTTGTTTAACTTTCTCG"
     misc_feature    2808..2856
                     /note="Geneious type: Recombinase Site"
                     /label="BxbiB"
     primer_bind     complement(2808..2856)
                     /label="BxbiB rev, 76.6"
     primer_bind     2808..2824
                     /label="Bxbi Fwd, 62.6"
     primer_bind     2827..2886
                     /label="BxbiB-RiboJ fwd, 67.5, 75.5"
     misc_feature    2857..3845
                     /note="Geneious type: Editing History Replacement"
                     /label="TCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAA
                     CAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTC
                     TAGCACGCGTACCATGGGATCCCCCGGGCTGCAGGAATTCGATATCAAGCTTTTATTT
                     GTATAGTTCATCCATGCCATGTGTAATCCCAGCAGCTGTTACAAACTCAAGAAGGACC
                     ATGTGGTCTCTCTTTTCGTTGGGATCTTTCGAAAGGGCAGATTGTGTGGACAGGTAAT
                     GGTTGTCTGGTAAAAGGACAGGGCCATCGCCAATTGGAGTATTTTGTTGATAATGGTC
                     TGCTAGTTGAACGCTTCCATCTTCAATGTTGTGTCTAATTTTGAAGTTAACTTTGATT
                     CCATTCTTTTGTTTGTCTGCCATGATGTATACATTGTGTGAGTTATAGTTGTATTCCA
                     ATTTGTGTCCAAGAATGTTTCCATCTTCTTTAAAATCAATACCTTTTAACTCGATTCT
                     ATTAACAAGGGTATCACCTTCAAACTTGACTTCAGCACGTGTCTTGTAGTTCCCGTCA
                     TCTTTGAAAAATATAGTTCTTTCCTGTACATAACCTTCGGGCATGGCACTCTTGAAAA
                     AGTCATGCTGTTTCATATGATCTGGGTATCTCGCAAAGCATTGAACACCATAACCGAA
                     AGTAGTGACAAGTGTTGGCCATGGAACAGGTAGTTTTCCAGTAGTGCAAATAAATTTA
                     AGGGTAAGTTTTCCGTATGTTGCATCACCTTCACCCTCTCCACTGACAGAAAATTTGT
                     GCCCATTAACATCACCATCTAATTCAACAAGAATTGGGACAACTCCAGTGAAAAGTTC
                     TTCTCCTTTACGCATCTTAAACCTCCTTACCTCGTAAACTATTAAACAAAATTATTTG
                     TAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCT
                     GTGCAC"
     misc_feature    2863..2937
                     /note="Geneious type: Ribozyme"
                     /label="RiboJ"
     mRNA            2870..2937
                     /label="mRNA"
     primer_bind     complement(2907..2951)
                     /label="60.7"
     primer_bind     complement(2907..2937)
                     /label="55.1"
     RBS             2938..2963
                     /label="RBS"
     primer_bind     2952..2991
                     /label="61.4"
     CDS             2964..3680
                     /label="gfp"
     primer_bind     2964..2991
                     /label="55.1"
     misc_feature    3129..3913
                     /note="Geneious type: try to seq"
                     /label=17
     misc_feature    3184..4120
                     /note="Geneious type: Verified by sequencing"
                     /label="E2"
     misc_feature    3710..>3839
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1)"
     terminator      3735..3839
                     /label="TermT1"
     misc_feature    <3735..>3839
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE11G - From Allen - Fragment 1 (concatenated sequence
                     1); Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1)"
     primer_bind     3735..3758
                     /label="TermT1 5' Forward"
     primer_bind     complement(3816..3875)
                     /label="(term)-(BxbiP), 64.3, 71.4"
     primer_bind     complement(3820..3839)
                     /label="59.7"
     primer_bind     complement(3828..3845)
                     /label="57.3"
     misc_feature    complement(3846..3903)
                     /note="Geneious type: Recombinase Site"
                     /label="BxbiP"
     primer_bind     3846..3903
                     /label="754, 72.9"
     primer_bind     complement(3846..3903)
                     /label="(BxbiP) rev, 72.9"
     primer_bind     complement(3882..3903)
                     /label
     misc_feature    3904
                     /note="Geneious type: Editing History Replacement"
                     /label="GC"
     primer_bind     3913..3942
                     /label="62.1"
     primer_bind     3913..3938
                     /label=61
     primer_bind     3914..3973
                     /label="3' (BxbiP) fwd, 69.8"
     misc_feature    complement(3924..4441)
                     /label="sopC/parC"
     misc_feature    complement(3965..3988)
                     /note="Geneious type: primer_bind_reverse"
                     /label="pBAC-step2-transf-bp2584-2561r  (D1277)"
     misc_feature    complement(4514..5485)
                     /label="sopB/parB"
     misc_feature    complement(5486..6651)
                     /label="sopA/parA"
     misc_feature    complement(6986..7236)
                     /gene="incC"
                     /note="incC; incompatibility region of the bacterial F
                     plasmid"
                     /label="incC"
     misc_feature    complement(7239..7994)
                     /label="BAC/F/repE"
     rep_origin      complement(8085..8304)
                     /note="also known as oriS; ori2; secondary origin of
                     replication for the bacterial F  plasmid"
                     /label="ori2 rep origin"
     rep_origin      8380..8994
                     /note="oriV; origin of replication for the bacterial F
                     plasmid"
                     /label="oriV rep origin"
     misc_feature    9829..680
                     /note="Geneious type: antibiotic resistance"
                     /label="CmR"
ORIGIN      
        1 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
       61 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      121 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      181 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
      241 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      301 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      361 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      421 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
      481 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
      541 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
      601 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
      661 cggggcgtaa gacgtctaag aaaccattat ctagggcggc ggatttgtcc tactcaggag
      721 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
      781 ttttatttga tgcctctagc acgcgtacca tgggatcccc cgggttactt gtacagctcg
      841 tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag
      901 tcctcgttgt gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc
      961 acgggcttct tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc
     1021 agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg
     1081 gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg
     1141 gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc
     1201 acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
     1261 aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg
     1321 tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc
     1381 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
     1441 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg
     1501 atggccatgt tatcttcttc gcccttgctc accattgtag acctccttat actttattgt
     1561 tagtttaaac aaaattattt gtagaggctg tttcgtcctc acggactcat cagaccggaa
     1621 agcacatccg gtgacagctg agctctgcgg gtgccagggc gtgcccttgg gctccccggg
     1681 cgcgtactcc atcgatttga gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac
     1741 tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaatag gtgaacatac
     1801 aaatgcggaa aagtttctgc cctcgcacgt agctgagcaa gtcgtagcca tgccccgcaa
     1861 acctggggta atcactgtat cgggctgtct aagagccgtt ggctgggcta gtcgtccatg
     1921 gcagtttgtc agttaggtca agatgcgggt ctctgtgtaa accggcccct ggggttttgg
     1981 cccaaactgc ctgatgctgc ataccgaata cacggtggtt aattgatgac tgcttgtgca
     2041 ctgctgcgcc ttgcggaggc cggtccggcg cgcgaagcgt ataaatgaag ctcgtcacat
     2101 ccacacagtt gcatcgtacg tcggtgtagg gatcgaactt ggcctctaat tccacatgcg
     2161 cgcgctgcag gtgtaagtgt gccaagtcac ctcgaccacg atgggctttg ccccaggtat
     2221 ccaaaaggaa atcctgatgc cgcgcttcga ttcaccgtag tgtggatgtt ccctttgtac
     2281 gggtttccga ctcctgttgt agcaggcgtc tttgttagct agcgctatcc ccaacgtgca
     2341 acaaccctta ccacgaagac aggattgtcc gatcctatat tacgactttg gcagggggtt
     2401 cgcaagtccc tgcaggaacg atgctgaagg ctcaggttac acaggcacaa gtactatata
     2461 tacgagatgc atctcttaac ctggatcgaa tgcagaatca tgaatcgtac cactgtgttc
     2521 gcctgcagct cgagcacagc taacaccacg tcgtccctat ctgctgccct aggtctatga
     2581 gtggttgctg gataacttta cgggcatgca taaggctcgt ataatatatt cagggagacc
     2641 acaacggttt ccctctacaa ataattttgt ttaactttgc tagcccccca actgagagaa
     2701 ctcaaaggtt accccagttg gggcacttag cccaatcttc aatacctcgt atgccgacaa
     2761 catggaccgg tcaccagcga gccctgtgcg gacgggaggt taattaacgg ccggcttgtc
     2821 gacgacggcg gtctccgtcg tcaggatcat ccgggcgtgc acagctgtca ccggatgtgc
     2881 tttccggtct gatgagtccg tgaggacgaa acagcctcta caaataattt tgtttaatag
     2941 tttacgaggt aaggaggttt aagatgagta aaggagaaga acttttcact ggagttgtcc
     3001 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     3061 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     3121 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
     3181 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
     3241 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     3301 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     3361 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     3421 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     3481 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     3541 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     3601 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     3661 tggatgatct ctacaaataa aagcttgata tcgaattcct gcagcccggg ggatcccatg
     3721 gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3781 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagaa
     3841 tcgatgtcgg ggtttgtacc gtacaccact gagaccgcgg tggttgacca gacaaaccac
     3901 gacacatgtc aatacttgcc cttgacaggc attgatggaa tcgtagtctc acgctgatag
     3961 tctgatcgac aatacaagtg ggaccgtggt cccagaccga taatcagacc gacaacacga
     4021 gtgggatcgt ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca
     4081 gactaataat cagaccgacg atacgagtgg gaccgtggtt ccagactaat aatcagaccg
     4141 acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc
     4201 atggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc cagtctgatt
     4261 atcagaccga cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg
     4321 agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc
     4381 agtctgatta tcagaccgac gatacaagtg gaacagtggg cccagagaga atattcaggc
     4441 cagttatgct ttctggcctg taacaaagga cattaagtaa agacagataa acgtagacta
     4501 aaacgtggtc gcatcagggt gctggctttt caagttcctt aagaatggcc tcaattttct
     4561 ctatacactc agttggaaca cgggacctgt ccaggttaag caccatttta tcgcccttat
     4621 acaatactgt cgctccagga gcaaactgat gtcgtgagct taaactagtt cttgatgcag
     4681 atgacgtttt aagcacagaa gttaaaagag tgataacttc ttcagcttca aatatcaccc
     4741 cagctttttt ctgctcatga aggttagatg cctgctgctt aagtaattcc tctttatctg
     4801 taaaggcttt ttgaagtgca tcacctgacc gggcagatag ttcaccgggg tgagaaaaaa
     4861 gagcaacaac tgatttaggc aatttggcgg tgttgataca gcgggtaata atcttacgtg
     4921 aaatattttc cgcatcagcc agcgcagaaa tatttccagc aaattcattc tgcaatcggc
     4981 ttgcataacg ctgaccacgt tcataagcac ttgttgggcg ataatcgtta cccaatctgg
     5041 ataatgcagc catctgctca tcatccagct cgccaaccag aacacgataa tcactttcgg
     5101 taagtgcagc agctttacga cggcgactcc catcggcaat ttctatgaca ccagatactc
     5161 ttcgaccgaa cgccggtgtc tgttgaccag tcagtagaaa agaagggatg agatcatcca
     5221 gtgcgtcctc agtaagcagc tcctggtcac gttcattacc tgaccatacc cgagaggtct
     5281 tctcaacact atcaccccgg agcacttcaa gagtaaactt cacatcccga ccacatacag
     5341 gcaaagtaat ggcattaccg cgagccatta ctcctacgcg cgcaattaac gaatccacca
     5401 tcggggcagc tggtgtcgat aacgaagtat cttcaaccgg ttgagtattg agcgtatgtt
     5461 ttggaataac aggcgcacgc ttcattatct aatctcccag cgtggtttaa tcagacgatc
     5521 gaaaatttca ttgcagacag gttcccaaat agaaagagca tttctccagg caccagttga
     5581 agagcgttga tcaatggcct gttcaaaaac agttctcatc cggatctgac ctttaccaac
     5641 ttcatccgtt tcacgtacaa cattttttag aaccatgctt ccccaggcat cccgaatttg
     5701 ctcctccatc cacggggact gagagccatt actattgctg tatttggtaa gcaaaatacg
     5761 tacatcaggc tcgaaccctt taagatcaac gttcttgagc agatcacgaa gcatatcgaa
     5821 aaactgcagt gcggaggtgt agtcaaacaa ctcagcaggc gtgggaacaa tcagcacatc
     5881 agcagcacat acgacattaa tcgtgccgat acccaggtta ggcgcgctgt caataactat
     5941 gacatcatag tcatgagcaa cagtttcaat ggccagtcgg agcatcaggt gtggatcggt
     6001 gggcagttta ccttcatcaa atttgcccat taactcagtt tcaatacggt gcagagccag
     6061 acaggaagga ataatgtcaa gccccggcca gcaagtgggc tttattgcat aagtgacatc
     6121 gtccttttcc ccaagataga aaggcaggag agtgtcttct gcatgaatat gaagatctgg
     6181 tacccatccg tgatacattg aggctgttcc ctgggggtcg ttaccttcca cgagcaaaac
     6241 acgtagcccc ttcagagcca gatcctgagc aagatgaaca gaaactgagg ttttgtaaac
     6301 gccaccttta tgggcagcaa ccccgatcac cggtggaaat acgtcttcag cacgtcgcaa
     6361 tcgcgtacca aacacatcac gcatatgatt aatttgttca attgtataac caacacgttg
     6421 ctcaacccgt cctcgaattt ccatatccgg gtgcggtagt cgccctgctt tctcggcatc
     6481 tctgatagcc tgagaagaaa ccccaactaa atccgctgct tcacctattc tccagcgccg
     6541 ggttattttc ctcgcttccg ggctgtcatc attaaactgt gcaatggcga tagccttcgt
     6601 catttcatga ccagcgttta tgcactggtt aagtgtttcc atgagtttca ttctgaacat
     6661 cctttaatca ttgctttgcg tttttttatt aaatcttgca atttactgca aagcaacaac
     6721 aaaatcgcaa agtcatcaaa aaaccgcaaa gttgtttaaa ataagagcaa cactacaaaa
     6781 ggagataaga agagcacata cctcagtcac ttattatcac tagcgctcgc cgcagccgtg
     6841 taaccgagca tagcgagcga actggcgagg aagcaaagaa gaactgttct gtcagatagc
     6901 tcttacgctc agcgcaagaa gaaatatcca ccgtgggaaa aactccaggt agaggtacac
     6961 acgcggatag ccaattcaga gtaataaact gtgataatca accctcatca atgatgacga
     7021 actaaccccc gatatcaggt cacatgacga agggaaagag aaggaaatca actgtgacaa
     7081 actgccctca aatttggctt ccttaaaaat tacagttcaa aaagtatgag aaaatccatg
     7141 caggctgaag gaaacagcaa aactgtgaca aattaccctc agtaggtcag aacaaatgtg
     7201 acgaaccacc ctcaaatctg tgacagataa ccctcagact atcctgtcgt catggaagtg
     7261 atatcgcgga aggaaaatac gatatgagtc gtctggcggc ctttcttttt ctcaatgtat
     7321 gagaggcgca ttggagttct gctgttgatc tcattaacac agacctgcag gaagcggcgg
     7381 cggaagtcag gcatacgctg gtaactttga ggcagctggt aacgctctat gatccagtcg
     7441 attttcagag agacgatgcc tgagccatcc ggcttacgat actgacacag ggattcgtat
     7501 aaacgcatgg catacggatt ggtgatttct tttgtttcac taagccgaaa ctgcgtaaac
     7561 cggttctgta acccgataaa gaagggaatg agatatgggt tgatatgtac actgtaaagc
     7621 cctctggatg gactgtgcgc acgtttgata aaccaaggaa aagattcata gcctttttca
     7681 tcgccggcat cctcttcagg gcgataaaaa accacttcct tccccgcgaa actcttcaat
     7741 gcctgccgta tatccttact ggcttccgca gaggtcaatc cgaatatttc agcatattta
     7801 gcaacatgga tctcgcagat accgtcatgt tcctgtaggg tgccatcaga ttttctgatc
     7861 tggtcaacga acagatacag catacgtttt tgatcccggg agagactata tgccgcctca
     7921 gtgaggtcgt ttgactggac gattcgcggg ctatttttac gtttcttgtg attgataacc
     7981 gctgtttccg ccatgacaga tccatgtgaa gtgtgacaag tttttagatt gtcacactaa
     8041 ataaaaaaga gtcaataagc agggataact ttgtgaaaaa acagcttctt ctgagggcaa
     8101 tttgtcacag ggttaagggc aatttgtcac agacaggact gtcatttgag ggtgatttgt
     8161 cacactgaaa gggcaatttg tcacaacacc ttctctagaa ccagcatgga taaaggccta
     8221 caaggcgctc taaaaaagaa gatctaaaaa ctataaaaaa aataattata aaaatatccc
     8281 cgtggataag tggataaccc caagggaagt tttttcaggc atcgtgtgta agcagaatat
     8341 ataagtgctg ttccctggtg cttcctcgct cactcgaccg ggagggttcg agaagggggg
     8401 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
     8461 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
     8521 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
     8581 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
     8641 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
     8701 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
     8761 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
     8821 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
     8881 aggtatccac aacgccggcg gccggccgcg gtgtctcgca cacggcttcg acggcgtttc
     8941 tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagccgagg
     9001 gcttcgccct gtcgctcgac tgcggcgagc actactggct gtaaaaggac agaccacatc
     9061 atggttctgt gttcattagg ttgttctgtc cattgctgac ataatccgct ccacttcaac
     9121 gtaacaccgc acgaagattt ctattgttcc tgaaggcata ttcaaatcgt tttcgttacc
     9181 gcttgcaggc atcatgacag aacactactt cctataaacg ctacacaggc tcctgagatt
     9241 aataatgcgg atctctacga taatgggaga ttttcccgac tgtttcgttc gcttctcagt
     9301 ggataacagc cagcttctct gtttaacaga caaaaacagc atatccactc agttccacat
     9361 ttccatataa aggccaaggc atttattctc aggataattg tttcagcatc gcaaccgcat
     9421 cagactccgg catcgcaaac tgcacccggt gccgggcagc cacatccagc gcaaaaacct
     9481 tcgtgtagac ttccgttgaa ctgatggact tatgtcccat caggctttgc agaactttca
     9541 gcggtatacc ggcatacagc atgtgcatcg cataggaatg gcggaacgta tgtggtgtga
     9601 ccggaacaga gaacgtcaca ccgtcagcag cagcggcggc aaccgcctcc ccaatccagg
     9661 tcctgaccgt tctgtccgtc acttcccaga tccgcgcttt ctctgtcctt cctgtgcgac
     9721 ggttacgccg ctccatgagc ttatcgcgaa taaatacctg tgacggaaga tcacttcgca
     9781 gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga
     9841 aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca
     9901 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaa
//
