LOCUS       ADC_BAC_Reporter       10630 bp    DNA     circular UNA 04-JUN-2015
DEFINITION  Gibson Assembly of
            pBAC-proD-PhiCB-(termT1)-(mCherry)-(RBS)-(RiboJ)-(PhiCP) extraction
            into pBAC-proD-BxbiB-(termT1)-(GFP)-(RBS_11_6_13B)-(RiboJ)-(BxbiP)
            extraction.
ACCESSION   urn.local...7e-4r2llbt
VERSION     urn.local...7e-4r2llbt
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     21..45
                     /label="63.2"
     promoter        27..170
                     /label="proD"
     primer_bind     27..66
                     /label="ProD Fwd, ORDER"
     primer_bind     complement(134..178)
                     /label="58.9"
     misc_feature    179..227
                     /note="Geneious type: Recombinase Site"
                     /label="BxbiB"
     primer_bind     complement(179..227)
                     /label="BxbiB rev, 76.6"
     primer_bind     179..195
                     /label="Bxbi Fwd, 62.6"
     primer_bind     228..245
                     /label="57.3"
     terminator      complement(234..338)
                     /label="TermT1"
     misc_feature    order(complement(<8278..>8382),complement(<234..>338))
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of
                     pZE2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 extraction extraction (concatenated sequence
                     1)"
     misc_feature    complement(<234..363)
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1)"
     misc_feature    complement(<234..>338)
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE11G - From Allen - Fragment 1 (concatenated sequence
                     1); Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1)"
     primer_bind     234..253
                     /label="59.7"
     primer_bind     complement(315..338)
                     /label="TermT1 5' Forward"
     CDS             complement(393..1109)
                     /label="gfp"
     misc_feature    494..1403
                     /note="Geneious type: try to seq"
                     /label=131
     misc_feature    536..1439
                     /note="Geneious type: Verified by sequencing"
                     /label="D18"
     misc_feature    547..1146
                     /note="Geneious type: Verified by sequencing"
                     /label="D26"
     misc_feature    548..1491
                     /note="Geneious type: verified by sequencing"
                     /label="F1"
     misc_feature    552..1327
                     /note="Geneious type: try to seq"
                     /label=131
     misc_feature    709..1481
                     /note="Geneious type: verified by sequencing"
                     /label="A14"
     misc_feature    878..1710
                     /note="Geneious type: Verified by sequencing"
                     /label="D18"
     misc_feature    898..1344
                     /note="Geneious type: geneblock"
                     /label="GENEBLOCK"
     primer_bind     complement(924..947)
                     /label="Primer 17 USE"
     primer_bind     complement(1082..1121)
                     /label="61.4"
     primer_bind     complement(1082..1109)
                     /label="55.1"
     RBS             complement(1110..1135)
                     /label="RBS"
     primer_bind     1122..1166
                     /label="60.7"
     misc_feature    complement(1136..1210)
                     /note="Geneious type: Ribozyme"
                     /label="RiboJ"
     mRNA            complement(1136..1203)
                     /label="mRNA"
     primer_bind     1136..1166
                     /label="55.1"
     misc_feature    complement(1217..1274)
                     /note="Geneious type: Recombinase Site"
                     /label="BxbiP"
     primer_bind     1217..1274
                     /label="754, 72.9"
     primer_bind     complement(1217..1274)
                     /label="(BxbiP) rev, 72.9"
     primer_bind     complement(1253..1274)
                     /label
     misc_feature    1275..1283
                     /note="Geneious type: Verified by sequencing"
                     /label="KEEP"
     misc_feature    1283^1284
                     /note="Geneious type: Editing History Deletion"
                     /label="CCTTTGGTCGAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTCTG
                     TGTTTCCCTCGAGCACAGCTAACACCACGTCGTCCCTATCTGCTGCCCTAGGTCTATG
                     AGTGGTTGCTGGATAACTTTACGGGCATGCATAAGGCTCGTATAATATATTCAGGGAG
                     ACCACAACGGTTTCCCTCTACAAATAATTTTGTTTAACTTTTTAATTAAATGCCAACA
                     CAATTAACATCTCAATCAAGGTAAATGCTTTTTGCTTTTTTTGCATCGATTCTAGGGC
                     GGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGG
                     CCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCACGCGTACCATGGGATCCC
                     CCGGGTTATTTGTACAATTCATCCATACCATGGGTAATACCAGCAGCAGTCCTAAATT
                     CTAACAGGACCATGTGGTCTCTCTTTTCGTTTGGATCTTTGGATAAGGCTGATTGGGT
                     GGATAAGTAATGGTTGTCTGGTAACAAGACTGGACCATCACCAATTGGAGTATTTTGT
                     TGATAATGGTCAGCTAATTGAACAGAACCATCTTCAATGTTGTGTCTAATTTTGAAGT
                     TCACTTTGATACCATTCTTTTGTTTGTCAGCCATGATGTATATATTGTGAGAGTTGAA
                     GTTGTATTCCAATTTGTGACCTAAAATGTTACCATCTTCTTTAAAATCAATACCTTTT
                     AATTCGATTCTATTAACTAAGGTATCACCTTCAAACTTGACTTCAGCTCTGGTCTTGT
                     AGTTACCGTCATCTTTGAAAAAAATAGTTCTTTCTTGAACATAACCTTCTGGCATGGC
                     AGACTTGAAAAAGTCATGTTGTTTCATATGATCTGGGTATCTAGAAAAACATTGAACA
                     CCATGGCTCAAAGTAGTTACTAAGGTTGGCCATGGAACTGGCAATTTACCAGTAGTAC
                     AAATAAATTTTAAGGTCAATTTACCGTACGTAGCATCACCTTCACCTTCACCGGAGAC
                     AGAAAATTTGTGACCATTAACATCACCATCTAATTCAACCAAAATTGGGACAACACCA
                     GTGAATAATTCTTCACCTTTAGACATTTTTAACCTCCTCCCTACGTACTCATTAAACA
                     AAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCC
                     GGTGACAGCTGTGTTTAAAGGAGTTTTTTAGTTACCTTAATTGAAATAAACGAAATAA
                     AAACTCGCCGAGCACCG"
     misc_feature    1284..1307
                     /note="Geneious type: Verified by sequencing"
                     /label="KEEP"
     primer_bind     complement(<1284..1334)
                     /label="3' (TP901P) rev, 70.9"
     primer_bind     1284..1313
                     /label="62.1"
     primer_bind     1284..1309
                     /label=61
     primer_bind     1285..1344
                     /label="3' (BxbiP) fwd, 69.8"
     primer_bind     1285..1344
                     /label="PRIMER (have)"
     misc_feature    complement(1295..1812)
                     /label="sopC/parC"
     misc_feature    complement(1336..1359)
                     /note="Geneious type: primer_bind_reverse"
                     /label="pBAC-step2-transf-bp2584-2561r  (D1277)"
     misc_feature    complement(1885..2856)
                     /label="sopB/parB"
     misc_feature    complement(2857..4022)
                     /label="sopA/parA"
     misc_feature    complement(4357..4607)
                     /gene="incC"
                     /note="incC; incompatibility region of the bacterial F
                     plasmid"
                     /label="incC"
     misc_feature    complement(4610..5365)
                     /label="BAC/F/repE"
     rep_origin      complement(5456..5675)
                     /note="also known as oriS; ori2; secondary origin of
                     replication for the bacterial F  plasmid"
                     /label="ori2 rep origin"
     rep_origin      5751..6365
                     /note="oriV; origin of replication for the bacterial F
                     plasmid"
                     /label="oriV rep origin"
     misc_feature    7200..7995
                     /note="Geneious type: antibiotic resistance"
                     /label="CmR"
     misc_feature    7315^7316
                     /note="Geneious type: origin"
                     /label="origin"
     primer_bind     complement(7879..7923)
                     /label="71.7"
     misc_feature    7899..>8271
                     /note="Geneious type: try to seq"
                     /label=185
                     7900..8004
     primer_bind     7900..7923
                     /label="pBAC-step2-transf-bp9125-9148f (D1276)"
     misc_feature    7923^7924
                     /note="Geneious type: Editing History Deletion"
                     /label="ATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGC"
     misc_feature    7952..8769
                     /note="Geneious type: Verified by sequencing"
                     /label="D18"
     misc_feature    7952..8769
                     /note="Geneious type: Verified by sequencing"
                     /label="D26"
     primer_bind     complement(7965..8004)
                     /label="69.1"
     primer_bind     7965..7989
                     /label="5' (termT1)-(mCherry)-(RBS)-(RiboJ) geneblock fwd,
                     69.2"
     primer_bind     complement(7969..8004)
                     /label=533
     primer_bind     complement(7980..8004)
                     /label="54.8"
     primer_bind     7987..8016
                     /label="54.7"
     primer_bind     complement(8047..8106)
                     /label="Ran-ProD Rev"
     primer_bind     8061..8085
                     /label="63.2"
     promoter        8067..8210
                     /label="proD"
     primer_bind     8067..8106
                     /label="ProD Fwd, ORDER"
     primer_bind     complement(8174..8263)
                     /label=68
     primer_bind     complement(8174..8218)
                     /label="58.9"
     misc_feature    8210^8211
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    8219..8271
                     /note="Geneious type: attB1"
                     /label="TP901B-TC"
     misc_feature    8219..8271
                     /note="Geneious type: recombinase site"
                     /label="TP901B"
     primer_bind     8219..8271
                     /label="TP901B fwd, 64.9"
     primer_bind     complement(8219..8271)
                     /label="TP901B rev"
     primer_bind     8272..8289
                     /label="57.3"
     terminator      complement(8278..8382)
                     /label="TermT1"
     misc_feature    order(complement(<9555..>9659),complement(<8278..>8382))
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of
                     pZE2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 extraction extraction (concatenated sequence
                     1)"
     misc_feature    complement(<8278..>8382)
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE11G - From Allen - Fragment 1 (concatenated sequence
                     1); Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1)"
     primer_bind     8278..8297
                     /label="59.7"
     primer_bind     complement(8359..8382)
                     /label="TermT1 5' Forward"
     misc_feature    8406
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of araC-Azurite extraction - Fragment 2
                     extraction (concatenated sequence 2)"
     CDS             complement(8407..9123)
                     /label="azurite"
     misc_feature    complement(8610..9488)
                     /note="Geneious type: Verified by sequencing"
                     /label="D18"
     RBS             complement(9124..9148)
                     /label="RBS"
     misc_feature    complement(9149..9223)
                     /note="Geneious type: Ribozyme"
                     /label="RiboJ"
     mRNA            complement(9149..9216)
                     /label="mRNA"
     primer_bind     9149..9179
                     /label="55.1"
     misc_feature    complement(9230..9279)
                     /note="Geneious type: attP1"
                     /label="TP901P-TC"
     misc_feature    complement(9230..9279)
                     /note="Geneious type: recombinase site"
                     /label="TP901P"
     primer_bind     complement(9230..9279)
                     /label="TP901P Fwd, order"
     primer_bind     complement(<9230..9279)
                     /label="67.7"
     misc_feature    9231..9730
                     /note="Geneious type: geneblock"
                     /label="Geneblcok"
     terminator      complement(9289..9345)
                     /label="ECK120033737"
     primer_bind     9346..9370
                     /label="63.2"
     promoter        9352..9495
                     /label="proD"
     primer_bind     9352..9391
                     /label="ProD Fwd, ORDER"
     primer_bind     complement(9459..9503)
                     /label="58.9"
     primer_bind     9493..9566
                     /label=76
     misc_feature    9504..9548
                     /note="Geneious type: Recombinase Site"
                     /label="PhiCB"
     primer_bind     9504..9533
                     /label="76.7"
     misc_feature    9545..10505
                     /note="Geneious type: Verified by sequencing"
                     /label="D26"
     misc_feature    9545..10485
                     /note="Geneious type: Verified by sequencing"
                     /label="D18"
     primer_bind     9549..9566
                     /label="57.3"
     terminator      complement(9555..9659)
                     /label="TermT1"
     misc_feature    complement(<9555..9684)
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1)"
     misc_feature    complement(<9555..>9659)
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE11G - From Allen - Fragment 1 (concatenated sequence
                     1); Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1)"
     primer_bind     9555..9574
                     /label="59.7"
     primer_bind     complement(9636..9659)
                     /label="TermT1 5' Forward"
     CDS             complement(9690..10403)
                     /label="mKate"
     misc_feature    9689^9690
                     /note="Geneious type: origin"
                     /label="origin"
     primer_bind     9690..9730
                     /label="mKate 3' Rev ORDER"
     misc_feature    complement(10346..10516)
                     /note="Geneious type: Verified by sequencing"
                     /label="D18"
     RBS             complement(10404..10431)
                     /label="RBS"
     misc_feature    complement(10432..10506)
                     /note="Geneious type: Ribozyme"
                     /label="RiboJ"
     mRNA            complement(10432..10499)
                     /label="mRNA"
     primer_bind     10432..10462
                     /label="55.1"
     misc_feature    complement(10513..10554)
                     /note="Geneious type: Recombinase Site"
                     /label="PhiCP"
     misc_feature    complement(10517..536)
                     /note="Geneious type: Verified by sequencing"
                     /label="D18"
     misc_feature    complement(10535..10554)
                     /note="Geneious type: primer_bind_reverse"
                     /label=61
     primer_bind     complement(10535..10559)
                     /label="67.6"
     primer_bind     complement(10541..10560)
                     /label="65.3"
     terminator      complement(10561..20)
                     /label="ECK120029600"
ORIGIN      
        1 tcttaagttt tttggctgaa ctcgagcaca gctaacacca cgtcgtccct atctgctgcc
       61 ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc gtataatata
      121 ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt ttaattaacg
      181 gccggcttgt cgacgacggc ggtctccgtc gtcaggatca tccgggcatc gattctaggg
      241 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
      301 ccagtctttc gactgagcct ttcgttttat ttgatgcctc tagcacgcgt accatgggat
      361 cccccgggct gcaggaattc gatatcaagc ttttatttgt agagatcatc catgccatgt
      421 gtaatcccag cagctgttac aaactcaaga aggaccatgt ggtctctctt ttcgttggga
      481 tctttcgaaa gggcagattg tgtggacagg taatggttgt ctggtaaaag gacagggcca
      541 tcgccaattg gagtattttg ttgataatgg tctgctagtt gaacgcttcc atcttcaatg
      601 ttgtgtctaa ttttgaagtt aactttgatt ccattctttt gtttgtctgc catgatgtat
      661 acattgtgtg agttatagtt gtattccaat ttgtgtccaa gaatgtttcc atcttcttta
      721 aaatcaatac cttttaactc gattctatta acaagggtat caccttcaaa cttgacttca
      781 gcacgtgtct tgtagttccc gtcatctttg aaaaatatag ttctttcctg tacataacct
      841 tcgggcatgg cactcttgaa aaagtcatgc tgtttcatat gatctgggta tctcgcaaag
      901 cattgaacac cataaccgaa agtagtgaca agtgttggcc atggaacagg tagttttcca
      961 gtagtgcaaa taaatttaag ggtaagtttt ccgtatgttg catcaccttc accctctcca
     1021 ctgacagaaa atttgtgccc attaacatca ccatctaatt caacaagaat tgggacaact
     1081 ccagtgaaaa gttcttctcc tttactcatc ttaaacctcc ttacctcgta aactattaaa
     1141 caaaattatt tgtagaggct gtttcgtcct cacggactca tcagaccgga aagcacatcc
     1201 ggtgacagct gtgcacgtcg gggtttgtac cgtacaccac tgagaccgcg gtggttgacc
     1261 agacaaacca cgacacatgt caatacttgc ccttgacagg cattgatgga atcgtagtct
     1321 cacgctgata gtctgatcga caatacaagt gggaccgtgg tcccagaccg ataatcagac
     1381 cgacaacacg agtgggatcg tggtcccaga ctaataatca gaccgacgat acgagtggga
     1441 ccgtggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt tccagactaa
     1501 taatcagacc gacgatacga gtgggaccgt ggtcccagac taataatcag accgacgata
     1561 cgagtgggac catggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtc
     1621 ccagtctgat tatcagaccg acgatacgag tgggaccgtg gtcccagact aataatcaga
     1681 ccgacgatac gagtgggacc gtggtcccag actaataatc agaccgacga tacgagtggg
     1741 accgtggtcc cagtctgatt atcagaccga cgatacaagt ggaacagtgg gcccagagag
     1801 aatattcagg ccagttatgc tttctggcct gtaacaaagg acattaagta aagacagata
     1861 aacgtagact aaaacgtggt cgcatcaggg tgctggcttt tcaagttcct taagaatggc
     1921 ctcaattttc tctatacact cagttggaac acgggacctg tccaggttaa gcaccatttt
     1981 atcgccctta tacaatactg tcgctccagg agcaaactga tgtcgtgagc ttaaactagt
     2041 tcttgatgca gatgacgttt taagcacaga agttaaaaga gtgataactt cttcagcttc
     2101 aaatatcacc ccagcttttt tctgctcatg aaggttagat gcctgctgct taagtaattc
     2161 ctctttatct gtaaaggctt tttgaagtgc atcacctgac cgggcagata gttcaccggg
     2221 gtgagaaaaa agagcaacaa ctgatttagg caatttggcg gtgttgatac agcgggtaat
     2281 aatcttacgt gaaatatttt ccgcatcagc cagcgcagaa atatttccag caaattcatt
     2341 ctgcaatcgg cttgcataac gctgaccacg ttcataagca cttgttgggc gataatcgtt
     2401 acccaatctg gataatgcag ccatctgctc atcatccagc tcgccaacca gaacacgata
     2461 atcactttcg gtaagtgcag cagctttacg acggcgactc ccatcggcaa tttctatgac
     2521 accagatact cttcgaccga acgccggtgt ctgttgacca gtcagtagaa aagaagggat
     2581 gagatcatcc agtgcgtcct cagtaagcag ctcctggtca cgttcattac ctgaccatac
     2641 ccgagaggtc ttctcaacac tatcaccccg gagcacttca agagtaaact tcacatcccg
     2701 accacataca ggcaaagtaa tggcattacc gcgagccatt actcctacgc gcgcaattaa
     2761 cgaatccacc atcggggcag ctggtgtcga taacgaagta tcttcaaccg gttgagtatt
     2821 gagcgtatgt tttggaataa caggcgcacg cttcattatc taatctccca gcgtggttta
     2881 atcagacgat cgaaaatttc attgcagaca ggttcccaaa tagaaagagc atttctccag
     2941 gcaccagttg aagagcgttg atcaatggcc tgttcaaaaa cagttctcat ccggatctga
     3001 cctttaccaa cttcatccgt ttcacgtaca acatttttta gaaccatgct tccccaggca
     3061 tcccgaattt gctcctccat ccacggggac tgagagccat tactattgct gtatttggta
     3121 agcaaaatac gtacatcagg ctcgaaccct ttaagatcaa cgttcttgag cagatcacga
     3181 agcatatcga aaaactgcag tgcggaggtg tagtcaaaca actcagcagg cgtgggaaca
     3241 atcagcacat cagcagcaca tacgacatta atcgtgccga tacccaggtt aggcgcgctg
     3301 tcaataacta tgacatcata gtcatgagca acagtttcaa tggccagtcg gagcatcagg
     3361 tgtggatcgg tgggcagttt accttcatca aatttgccca ttaactcagt ttcaatacgg
     3421 tgcagagcca gacaggaagg aataatgtca agccccggcc agcaagtggg ctttattgca
     3481 taagtgacat cgtccttttc cccaagatag aaaggcagga gagtgtcttc tgcatgaata
     3541 tgaagatctg gtacccatcc gtgatacatt gaggctgttc cctgggggtc gttaccttcc
     3601 acgagcaaaa cacgtagccc cttcagagcc agatcctgag caagatgaac agaaactgag
     3661 gttttgtaaa cgccaccttt atgggcagca accccgatca ccggtggaaa tacgtcttca
     3721 gcacgtcgca atcgcgtacc aaacacatca cgcatatgat taatttgttc aattgtataa
     3781 ccaacacgtt gctcaacccg tcctcgaatt tccatatccg ggtgcggtag tcgccctgct
     3841 ttctcggcat ctctgatagc ctgagaagaa accccaacta aatccgctgc ttcacctatt
     3901 ctccagcgcc gggttatttt cctcgcttcc gggctgtcat cattaaactg tgcaatggcg
     3961 atagccttcg tcatttcatg accagcgttt atgcactggt taagtgtttc catgagtttc
     4021 attctgaaca tcctttaatc attgctttgc gtttttttat taaatcttgc aatttactgc
     4081 aaagcaacaa caaaatcgca aagtcatcaa aaaaccgcaa agttgtttaa aataagagca
     4141 acactacaaa aggagataag aagagcacat acctcagtca cttattatca ctagcgctcg
     4201 ccgcagccgt gtaaccgagc atagcgagcg aactggcgag gaagcaaaga agaactgttc
     4261 tgtcagatag ctcttacgct cagcgcaaga agaaatatcc accgtgggaa aaactccagg
     4321 tagaggtaca cacgcggata gccaattcag agtaataaac tgtgataatc aaccctcatc
     4381 aatgatgacg aactaacccc cgatatcagg tcacatgacg aagggaaaga gaaggaaatc
     4441 aactgtgaca aactgccctc aaatttggct tccttaaaaa ttacagttca aaaagtatga
     4501 gaaaatccat gcaggctgaa ggaaacagca aaactgtgac aaattaccct cagtaggtca
     4561 gaacaaatgt gacgaaccac cctcaaatct gtgacagata accctcagac tatcctgtcg
     4621 tcatggaagt gatatcgcgg aaggaaaata cgatatgagt cgtctggcgg cctttctttt
     4681 tctcaatgta tgagaggcgc attggagttc tgctgttgat ctcattaaca cagacctgca
     4741 ggaagcggcg gcggaagtca ggcatacgct ggtaactttg aggcagctgg taacgctcta
     4801 tgatccagtc gattttcaga gagacgatgc ctgagccatc cggcttacga tactgacaca
     4861 gggattcgta taaacgcatg gcatacggat tggtgatttc ttttgtttca ctaagccgaa
     4921 actgcgtaaa ccggttctgt aacccgataa agaagggaat gagatatggg ttgatatgta
     4981 cactgtaaag ccctctggat ggactgtgcg cacgtttgat aaaccaagga aaagattcat
     5041 agcctttttc atcgccggca tcctcttcag ggcgataaaa aaccacttcc ttccccgcga
     5101 aactcttcaa tgcctgccgt atatccttac tggcttccgc agaggtcaat ccgaatattt
     5161 cagcatattt agcaacatgg atctcgcaga taccgtcatg ttcctgtagg gtgccatcag
     5221 attttctgat ctggtcaacg aacagataca gcatacgttt ttgatcccgg gagagactat
     5281 atgccgcctc agtgaggtcg tttgactgga cgattcgcgg gctattttta cgtttcttgt
     5341 gattgataac cgctgtttcc gccatgacag atccatgtga agtgtgacaa gtttttagat
     5401 tgtcacacta aataaaaaag agtcaataag cagggataac tttgtgaaaa aacagcttct
     5461 tctgagggca atttgtcaca gggttaaggg caatttgtca cagacaggac tgtcatttga
     5521 gggtgatttg tcacactgaa agggcaattt gtcacaacac cttctctaga accagcatgg
     5581 ataaaggcct acaaggcgct ctaaaaaaga agatctaaaa actataaaaa aaataattat
     5641 aaaaatatcc ccgtggataa gtggataacc ccaagggaag ttttttcagg catcgtgtgt
     5701 aagcagaata tataagtgct gttccctggt gcttcctcgc tcactcgacc gggagggttc
     5761 gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt
     5821 taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc
     5881 cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa
     5941 atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg
     6001 cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca
     6061 ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg
     6121 aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg
     6181 cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca
     6241 agttttccgc gaggtatcca caacgccggc ggccggccgc ggtgtctcgc acacggcttc
     6301 gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca
     6361 accagccgag ggcttcgccc tgtcgctcga ctgcggcgag cactactggc tgtaaaagga
     6421 cagaccacat catggttctg tgttcattag gttgttctgt ccattgctga cataatccgc
     6481 tccacttcaa cgtaacaccg cacgaagatt tctattgttc ctgaaggcat attcaaatcg
     6541 ttttcgttac cgcttgcagg catcatgaca gaacactact tcctataaac gctacacagg
     6601 ctcctgagat taataatgcg gatctctacg ataatgggag attttcccga ctgtttcgtt
     6661 cgcttctcag tggataacag ccagcttctc tgtttaacag acaaaaacag catatccact
     6721 cagttccaca tttccatata aaggccaagg catttattct caggataatt gtttcagcat
     6781 cgcaaccgca tcagactccg gcatcgcaaa ctgcacccgg tgccgggcag ccacatccag
     6841 cgcaaaaacc ttcgtgtaga cttccgttga actgatggac ttatgtccca tcaggctttg
     6901 cagaactttc agcggtatac cggcatacag catgtgcatc gcataggaat ggcggaacgt
     6961 atgtggtgtg accggaacag agaacgtcac accgtcagca gcagcggcgg caaccgcctc
     7021 cccaatccag gtcctgaccg ttctgtccgt cacttcccag atccgcgctt tctctgtcct
     7081 tcctgtgcga cggttacgcc gctccatgag cttatcgcga ataaatacct gtgacggaag
     7141 atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
     7201 acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
     7261 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     7321 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     7381 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     7441 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     7501 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag
     7561 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     7621 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     7681 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     7741 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     7801 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     7861 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     7921 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     7981 cgtaagacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8041 cgaggccctt tcgtcttcac ctcgagcaca gctaacacca cgtcgtccct atctgctgcc
     8101 ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc gtataatata
     8161 ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt ttaattaaat
     8221 gccaacacaa ttaacatctc aatcaaggta aatgcttttt gctttttttg catcgattct
     8281 agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     8341 aggcccagtc tttcgactga gcctttcgtt ttatttgatg ccacgcgtac catgggatcc
     8401 cccgggttat ttgtacaatt catccatacc atgggtaata ccagcagcag tcctaaattc
     8461 taacaggacc atgtggtctc tcttttcgtt tggatctttg gataaggctg attgggtgga
     8521 taagtaatgg ttgtctggta acaagactgg accatcacca attggagtat tttgttgata
     8581 atggtcagct aattgaacag aaccatcttc aatgttgtgt ctaattttga agttcacttt
     8641 gataccattc ttttgtttgt cagccatgat gtatatattg tgagagttga agttgtattc
     8701 caatttgtga cctaaaatgt taccatcttc tttaaaatca atacctttta attcgattct
     8761 attaactaag gtatcacctt caaacttgac ttcagctctg gtcttgtagt taccgtcatc
     8821 tttgaaaaaa atagttcttt cttgaacata accttctggc atggcagact tgaaaaagtc
     8881 atgttgtttc atatgatctg ggtatctaga aaaacattga acaccatggc tcaaagtagt
     8941 tactaaggtt ggccatggaa ctggcaattt accagtagta caaataaatt ttaaggtcaa
     9001 tttaccgtac gtagcatcac cttcaccttc accggagaca gaaaatttgt gaccattaac
     9061 atcaccatct aattcaacca aaattgggac aacaccagtg aataattctt cacctttaga
     9121 catttttaac ctcctcccta cgtactcatt aaacaaaatt atttgtagag gctgtttcgt
     9181 cctcacggac tcatcagacc ggaaagcaca tccggtgaca gctgtgttta aaggagtttt
     9241 ttagttacct taattgaaat aaacgaaata aaaactcgcc gagcaccgcc tttggtcgaa
     9301 aaaaaaagcc cgcactgtca ggtgcgggct tttttctgtg tttccctcga gcacagctaa
     9361 caccacgtcg tccctatctg ctgccctagg tctatgagtg gttgctggat aactttacgg
     9421 gcatgcataa ggctcgtata atatattcag ggagaccaca acggtttccc tctacaaata
     9481 attttgttta actttttaat taatgcgggt gccagggcgt gcccttgggc tccccgggcg
     9541 cgtactccat cgattctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa
     9601 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     9661 ctagcacgcg taccatggga tcccccgggt tagttcagtt tgtggcccag tttggaaggc
     9721 agatcgcaat agcgtgccac tgccacttca tgctgttcga cataggtttc tttgtccgct
     9781 tctttgatac gttccagacg gcggtcgaca taatatacgc ccggcatttt caggttttta
     9841 gccggttttt tgctgcggta agtagttttc aggttacaga tcaggtggcc gccaccaacc
     9901 agtttcagcg ccatatcaga acggccttcc aggccgccgt cagccgggta cagcatttcg
     9961 gtggatgctt cccagcccag cgtttttttc tgcataaccg ggccgttgct cgggaaatta
    10021 acaccacgaa ttttaacgtt atagatcaga cagccatcct gcagagacgt gtcctgcgta
    10081 gcggtcagga cgccaccatc ttcatacgtc gtaacgcgtt cccaggtaaa gccctccggg
    10141 aagctctgtt tgaaaaaatc cgggatacct tgagtgtggt tgatgaaggt tttgctgccg
    10201 tacatgaagc tggtcgccag gatgtcgaat gcaaacggca gcggaccgcc ttccacaact
    10261 ttgatacgca tggtttgggt gccctcatac ggtttgcctt cgccttcgct ggtacatttg
    10321 aagtggtggt tgtttacagt accttccatg tacagcttca tgtgcatgtt ttctttaatc
    10381 agttcttcac ctttggaaac catttttacc tccttattaa ttttatgtcg tttaaacaaa
    10441 attatttgta gaggctgttt cgtcctcacg gactcatcag accggaaagc acatccggtg
    10501 acagctgtgc accccccaac tgagagaact caaaggttac cccagttggg gcaccgagca
    10561 ttgagaagag aaaagaaaac cgccgatcct gtccaccgca ttactgcaag gtagtggaca
    10621 agaccggcgg
//
