LOCUS       Mixed-signal_int        9097 bp    DNA     circular UNA 04-JUN-2015
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...84-4r2mgw3
VERSION     urn.local...84-4r2mgw3
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     complement(564..608)
                     /label="71.7"
                     585..689
     misc_feature    585..1441
                     /note="Geneious type: try to seq"
                     /label=185
     primer_bind     585..608
                     /label="pBAC-step2-transf-bp9125-9148f (D1276)"
     misc_feature    588..1454
                     /note="Geneious type: try to seq"
                     /label=185
     misc_feature    636..1478
                     /note="Geneious type: verified by sequencing"
                     /label="F1"
     misc_feature    640..1449
                     /note="Geneious type: Verified by sequencing"
                     /label="A4"
     misc_feature    650..1826
                     /note="Geneious type: geneblock"
                     /label="geneblock"
     primer_bind     complement(650..689)
                     /label="69.1"
     primer_bind     650..674
                     /label="5' (termT1)-(mCherry)-(RBS)-(RiboJ) geneblock fwd,
                     69.2"
     primer_bind     complement(654..689)
                     /label=533
     primer_bind     complement(665..689)
                     /label="54.8"
     misc_feature    672..675
                     /note="Geneious type: ligation"
                     /label="Ligation"
     misc_feature    690..742
                     /note="Geneious type: attB1"
                     /label="TP901B-TC"
     misc_feature    690..1786
                     /note="Geneious type: Editing History Insertion"
                     /label
     misc_feature    690..742
                     /note="Geneious type: recombinase site"
                     /label="TP901B"
     primer_bind     690..742
                     /label="TP901B fwd, 64.9"
     primer_bind     complement(690..742)
                     /label="TP901B rev"
     primer_bind     747..771
                     /label="54.3"
     terminator      complement(749..838)
                     /note="Geneious type: Terminator"
                     /label="ECK120029600"
     primer_bind     839..878
                     /label="65.7"
     misc_feature    complement(1402..2280)
                     /note="Geneious type: Verified by sequencing"
                     /label="A4"
     misc_feature    complement(1462..2321)
                     /note="Geneious type: try to seq"
                     /label=130
     primer_bind     1581..1605
                     /label="63.2"
     promoter        1587..1730
                     /note="Geneious type: Promoter"
                     /label="ProD"
     primer_bind     1587..1626
                     /label="ProD Fwd, ORDER"
     primer_bind     complement(1704..1736)
                     /label="55.6"
     misc_feature    complement(1737..1786)
                     /note="Geneious type: attP1"
                     /label="TP901P-TC"
     misc_feature    complement(1737..1786)
                     /note="Geneious type: recombinase site"
                     /label="TP901P"
     primer_bind     complement(<1737..1786)
                     /label="67.7"
     primer_bind     complement(1737..1786)
                     /label="TP901P Fwd, order"
     misc_feature    complement(1759..2637)
                     /note="Geneious type: Verified by sequencing"
                     /label="A4"
     primer_bind     1787..1826
                     /label="65.8"
     misc_feature    1790..1911
                     /note="Geneious type: Editing History Replacement"
                     /label="TCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTT
                     CACCTCGAGCACAGCTAACACCACGTCGTCCCTATCTGCTGCCCTAGGTCTATGAGTG
                     GTTGCTGGATAACTTTACGGGCATGCATAAGGCTCGTATAATATATTCAGGGAGACCA
                     CAACGGTTTCCCTCTACAAATAATTTTGTTTAACTTTCTCG"
     primer_bind     complement(1840..1899)
                     /label="82.3"
     primer_bind     complement(1840..1859)
                     /label="67.1"
     misc_feature    1860..1904
                     /note="Geneious type: Recombinase Site"
                     /label="PhiCB"
     primer_bind     1860..1889
                     /label="76.7"
     primer_bind     1870..1929
                     /label="76.9"
     misc_feature    1913..1961
                     /note="Geneious type: Recombinase Site"
                     /label="BxbiB"
     primer_bind     complement(1913..1961)
                     /label="BxbiB rev, 76.6"
     primer_bind     1913..1929
                     /label="Bxbi Fwd, 62.6"
     misc_feature    1962..1967
                     /note="Geneious type: Editing History Insertion"
                     /label
     primer_bind     1962..1979
                     /label="57.3"
     misc_feature    complement(<1968..2097)
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1)"
     misc_feature    complement(<1968..>2072)
                     /note="Geneious type: concatenated sequence"
                     /label="Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE11G - From Allen - Fragment 1 (concatenated sequence
                     1); Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE11G - From Allen - Fragment 1 (concatenated sequence
                     1); Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)
                      - Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE11G - From Allen - Fragment 1 (concatenated sequence
                     1); Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)
                      - Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of pZE11G - From
                     Allen - Fragment 1 (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE11G - From Allen - Fragment 1
                     (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of pZE11G
                     - From Allen - Fragment 1 (concatenated sequence 1);
                     Digestion of pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  -
                     Fragment 3 extraction extraction (concatenated sequence
                     1); Digestion of
                     pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1); Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1); Digestion
                     of pZS2pLtetO350-mCherry-trunc_pLtetO-RBS0029-tetR(V) -
                     Fragment 2 - Digestion of
                     pZE2pLtetO350-mCherry-proA-RS0029-tetR(V)  - Fragment 3
                     extraction extraction (concatenated sequence 1) extraction
                     extraction (concatenated sequence 1)"
     terminator      complement(1968..2072)
                     /label="Term"
     primer_bind     1968..1987
                     /label="59.7"
     primer_bind     complement(2049..2072)
                     /label="TermT1 5' Forward"
     misc_feature    2072^2073
                     /note="Geneious type: Editing History Deletion"
                     /label="T"
     CDS             complement(2127..2843)
                     /label="gfp"
     misc_feature    2136
                     /note="Geneious type: Editing History Replacement"
                     /label="T"
     misc_feature    2139
                     /note="Geneious type: Editing History Replacement"
                     /label="T"
     misc_feature    2281..2880
                     /note="Geneious type: verified by sequencing"
                     /label="D26"
     misc_feature    2568..3364
                     /note="Geneious type: try to seq"
                     /label=18
     misc_feature    2612..3566
                     /note="Geneious type: Verified by sequencing"
                     /label=18
     primer_bind     complement(2658..2681)
                     /label="Primer 17 USE"
     primer_bind     complement(2816..2855)
                     /label="61.4"
     primer_bind     complement(2816..2843)
                     /label="55.1"
     misc_feature    2840
                     /note="Geneious type: Editing History Replacement"
                     /label="G"
     RBS             complement(2844..2869)
                     /label="RBS"
     primer_bind     2856..2900
                     /label="60.7"
     misc_feature    complement(2870..2944)
                     /note="Geneious type: Ribozyme"
                     /label="RiboJ"
     mRNA            complement(2870..2937)
                     /label="mRNA"
     primer_bind     2870..2900
                     /label="55.1"
     misc_feature    complement(2951..3008)
                     /note="Geneious type: Recombinase Site"
                     /label="BxbiP"
     primer_bind     2951..3008
                     /label="754, 72.9"
     primer_bind     complement(2951..3008)
                     /label="(BxbiP) rev, 72.9"
     primer_bind     complement(2987..3008)
                     /label
     primer_bind     complement(2990..3049)
                     /label="71.3"
     primer_bind     complement(2990..3014)
                     /label="60.6"
     misc_feature    3009
                     /note="Geneious type: Editing History Replacement"
                     /label="GC"
     misc_feature    complement(3015..3056)
                     /note="Geneious type: Recombinase Site"
                     /label="PhiCP"
     misc_feature    3016..3065
                     /note="Geneious type: Editing History Replacement"
                     /label="AA"
     primer_bind     3029..3088
                     /label="73.6"
     primer_bind     complement(3036..3095)
                     /label=803
     primer_bind     complement(3036..3065)
                     /label="71.2"
     misc_feature    complement(3037..3056)
                     /note="Geneious type: primer_bind_reverse"
                     /label=61
     primer_bind     complement(3037..3061)
                     /label="67.6"
     primer_bind     complement(3043..3062)
                     /label="65.3"
     primer_bind     3057..3081
                     /label="63.4"
     primer_bind     3062..3091
                     /label="65.1"
     misc_feature    3066..3089
                     /note="Geneious type: verified by sequencing"
                     /label="KEEP"
     primer_bind     complement(<3066..3116)
                     /label="3' (TP901P) rev, 70.9"
     primer_bind     3066..3095
                     /label="62.1"
     primer_bind     3066..3091
                     /label=61
     primer_bind     3067..3126
                     /label="3' (BxbiP) fwd, 69.8; PRIMER (have)"
     misc_feature    complement(3077..3594)
                     /label="sopC/parC"
     misc_feature    complement(3118..3141)
                     /note="Geneious type: primer_bind_reverse"
                     /label="pBAC-step2-transf-bp2584-2561r  (D1277)"
     misc_feature    complement(3667..4638)
                     /label="sopB/parB"
     misc_feature    complement(4639..5804)
                     /label="sopA/parA"
     misc_feature    complement(6139..6389)
                     /gene="incC"
                     /note="incC; incompatibility region of the bacterial F
                     plasmid"
                     /label="incC"
     misc_feature    complement(6392..7147)
                     /label="BAC/F/repE"
     rep_origin      complement(7238..7457)
                     /note="also known as oriS; ori2; secondary origin of
                     replication for the bacterial F  plasmid"
                     /label="ori2 rep origin"
     rep_origin      7533..8147
                     /note="oriV; origin of replication for the bacterial F
                     plasmid"
                     /label="oriV rep origin"
     misc_feature    8982..680
                     /note="Geneious type: antibiotic resistance"
                     /label="CmR"
ORIGIN      
        1 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
       61 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      121 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      181 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
      241 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      301 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      361 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      421 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
      481 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
      541 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
      601 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
      661 cggggcgtaa gacgtctaag aaaccattaa tgccaacaca attaacatct caatcaaggt
      721 aaatgctttt tgcttttttt gcatcgattt gagaagagaa aagaaaaccg ccgatcctgt
      781 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaat
      841 aggtgaacat acaaatgcgg aaaagtttct gccctcgcac gtagctgagc aagtcgtagc
      901 catgccccgc aaacctgggg taatcactgt atcgggctgt ctaagagccg ttggctgggc
      961 tagtcgtcca tggcagtttg tcagttaggt caagatgcgg gtctctgtgt aaaccggccc
     1021 ctggggtttt ggcccaaact gcctgatgct gcataccgaa tacacggtgg ttaattgatg
     1081 actgcttgtg cactgctgcg ccttgcggag gccggtccgg cgcgcgaagc gtataaatga
     1141 agctcgtcac atccacacag ttgcatcgta cgtcggtgta gggatcgaac ttggcctcta
     1201 attccacatg cgcgcgctgc aggtgtaagt gtgccaagtc acctcgacca cgatgggctt
     1261 tgccccaggt atccaaaagg aaatcctgat gccgcgcttc gattcaccgt agtgtggatg
     1321 ttccctttgt acgggtttcc gactcctgtt gtagcaggcg tctttgttag ctagcgctat
     1381 ccccaacgtg caacaaccct taccacgaag acaggattgt ccgatcctat attacgactt
     1441 tggcaggggg ttcgcaagtc cctgcaggaa cgatgctgaa ggctcaggtt acacaggcac
     1501 aagtactata tatacgagat gcatctctta acctggatcg aatgcagaat catgaatcgt
     1561 accactgtgt tcgcctgcag ctcgagcaca gctaacacca cgtcgtccct atctgctgcc
     1621 ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc gtataatata
     1681 ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt gctagcaaag
     1741 gagtttttta gttaccttaa ttgaaataaa cgaaataaaa actcgcttag cccaatcttc
     1801 aatacctcgt atgccgacaa catggaccgg tcaccagcga gccctgtgcg gacgggaggt
     1861 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccttaatt aacggccggc
     1921 ttgtcgacga cggcggtctc cgtcgtcagg atcatccggg catcgattct agggcggcgg
     1981 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     2041 tttcgactga gcctttcgtt ttatttgatg cctctagcac gcgtaccatg ggatcccccg
     2101 ggctgcagga attcgatatc aagcttttat ttgtagagat catccatgcc atgtgtaatc
     2161 ccagcagctg ttacaaactc aagaaggacc atgtggtctc tcttttcgtt gggatctttc
     2221 gaaagggcag attgtgtgga caggtaatgg ttgtctggta aaaggacagg gccatcgcca
     2281 attggagtat tttgttgata atggtctgct agttgaacgc ttccatcttc aatgttgtgt
     2341 ctaattttga agttaacttt gattccattc ttttgtttgt ctgccatgat gtatacattg
     2401 tgtgagttat agttgtattc caatttgtgt ccaagaatgt ttccatcttc tttaaaatca
     2461 atacctttta actcgattct attaacaagg gtatcacctt caaacttgac ttcagcacgt
     2521 gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt cctgtacata accttcgggc
     2581 atggcactct tgaaaaagtc atgctgtttc atatgatctg ggtatctcgc aaagcattga
     2641 acaccataac cgaaagtagt gacaagtgtt ggccatggaa caggtagttt tccagtagtg
     2701 caaataaatt taagggtaag ttttccgtat gttgcatcac cttcaccctc tccactgaca
     2761 gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac aactccagtg
     2821 aaaagttctt ctcctttact catcttaaac ctccttacct cgtaaactat taaacaaaat
     2881 tatttgtaga ggctgtttcg tcctcacgga ctcatcagac cggaaagcac atccggtgac
     2941 agctgtgcac gtcggggttt gtaccgtaca ccactgagac cgcggtggtt gaccagacaa
     3001 accacgacac atgtccccca actgagagaa ctcaaaggtt accccagttg gggcaccgag
     3061 caccgtactt gcccttgaca ggcattgatg gaatcgtagt ctcacgctga tagtctgatc
     3121 gacaatacaa gtgggaccgt ggtcccagac cgataatcag accgacaaca cgagtgggat
     3181 cgtggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat
     3241 aatcagaccg acgatacgag tgggaccgtg gttccagact aataatcaga ccgacgatac
     3301 gagtgggacc gtggtcccag actaataatc agaccgacga tacgagtggg accatggtcc
     3361 cagactaata atcagaccga cgatacgagt gggaccgtgg tcccagtctg attatcagac
     3421 cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga
     3481 ccgtggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagtctga
     3541 ttatcagacc gacgatacaa gtggaacagt gggcccagag agaatattca ggccagttat
     3601 gctttctggc ctgtaacaaa ggacattaag taaagacaga taaacgtaga ctaaaacgtg
     3661 gtcgcatcag ggtgctggct tttcaagttc cttaagaatg gcctcaattt tctctataca
     3721 ctcagttgga acacgggacc tgtccaggtt aagcaccatt ttatcgccct tatacaatac
     3781 tgtcgctcca ggagcaaact gatgtcgtga gcttaaacta gttcttgatg cagatgacgt
     3841 tttaagcaca gaagttaaaa gagtgataac ttcttcagct tcaaatatca ccccagcttt
     3901 tttctgctca tgaaggttag atgcctgctg cttaagtaat tcctctttat ctgtaaaggc
     3961 tttttgaagt gcatcacctg accgggcaga tagttcaccg gggtgagaaa aaagagcaac
     4021 aactgattta ggcaatttgg cggtgttgat acagcgggta ataatcttac gtgaaatatt
     4081 ttccgcatca gccagcgcag aaatatttcc agcaaattca ttctgcaatc ggcttgcata
     4141 acgctgacca cgttcataag cacttgttgg gcgataatcg ttacccaatc tggataatgc
     4201 agccatctgc tcatcatcca gctcgccaac cagaacacga taatcacttt cggtaagtgc
     4261 agcagcttta cgacggcgac tcccatcggc aatttctatg acaccagata ctcttcgacc
     4321 gaacgccggt gtctgttgac cagtcagtag aaaagaaggg atgagatcat ccagtgcgtc
     4381 ctcagtaagc agctcctggt cacgttcatt acctgaccat acccgagagg tcttctcaac
     4441 actatcaccc cggagcactt caagagtaaa cttcacatcc cgaccacata caggcaaagt
     4501 aatggcatta ccgcgagcca ttactcctac gcgcgcaatt aacgaatcca ccatcggggc
     4561 agctggtgtc gataacgaag tatcttcaac cggttgagta ttgagcgtat gttttggaat
     4621 aacaggcgca cgcttcatta tctaatctcc cagcgtggtt taatcagacg atcgaaaatt
     4681 tcattgcaga caggttccca aatagaaaga gcatttctcc aggcaccagt tgaagagcgt
     4741 tgatcaatgg cctgttcaaa aacagttctc atccggatct gacctttacc aacttcatcc
     4801 gtttcacgta caacattttt tagaaccatg cttccccagg catcccgaat ttgctcctcc
     4861 atccacgggg actgagagcc attactattg ctgtatttgg taagcaaaat acgtacatca
     4921 ggctcgaacc ctttaagatc aacgttcttg agcagatcac gaagcatatc gaaaaactgc
     4981 agtgcggagg tgtagtcaaa caactcagca ggcgtgggaa caatcagcac atcagcagca
     5041 catacgacat taatcgtgcc gatacccagg ttaggcgcgc tgtcaataac tatgacatca
     5101 tagtcatgag caacagtttc aatggccagt cggagcatca ggtgtggatc ggtgggcagt
     5161 ttaccttcat caaatttgcc cattaactca gtttcaatac ggtgcagagc cagacaggaa
     5221 ggaataatgt caagccccgg ccagcaagtg ggctttattg cataagtgac atcgtccttt
     5281 tccccaagat agaaaggcag gagagtgtct tctgcatgaa tatgaagatc tggtacccat
     5341 ccgtgataca ttgaggctgt tccctggggg tcgttacctt ccacgagcaa aacacgtagc
     5401 cccttcagag ccagatcctg agcaagatga acagaaactg aggttttgta aacgccacct
     5461 ttatgggcag caaccccgat caccggtgga aatacgtctt cagcacgtcg caatcgcgta
     5521 ccaaacacat cacgcatatg attaatttgt tcaattgtat aaccaacacg ttgctcaacc
     5581 cgtcctcgaa tttccatatc cgggtgcggt agtcgccctg ctttctcggc atctctgata
     5641 gcctgagaag aaaccccaac taaatccgct gcttcaccta ttctccagcg ccgggttatt
     5701 ttcctcgctt ccgggctgtc atcattaaac tgtgcaatgg cgatagcctt cgtcatttca
     5761 tgaccagcgt ttatgcactg gttaagtgtt tccatgagtt tcattctgaa catcctttaa
     5821 tcattgcttt gcgttttttt attaaatctt gcaatttact gcaaagcaac aacaaaatcg
     5881 caaagtcatc aaaaaaccgc aaagttgttt aaaataagag caacactaca aaaggagata
     5941 agaagagcac atacctcagt cacttattat cactagcgct cgccgcagcc gtgtaaccga
     6001 gcatagcgag cgaactggcg aggaagcaaa gaagaactgt tctgtcagat agctcttacg
     6061 ctcagcgcaa gaagaaatat ccaccgtggg aaaaactcca ggtagaggta cacacgcgga
     6121 tagccaattc agagtaataa actgtgataa tcaaccctca tcaatgatga cgaactaacc
     6181 cccgatatca ggtcacatga cgaagggaaa gagaaggaaa tcaactgtga caaactgccc
     6241 tcaaatttgg cttccttaaa aattacagtt caaaaagtat gagaaaatcc atgcaggctg
     6301 aaggaaacag caaaactgtg acaaattacc ctcagtaggt cagaacaaat gtgacgaacc
     6361 accctcaaat ctgtgacaga taaccctcag actatcctgt cgtcatggaa gtgatatcgc
     6421 ggaaggaaaa tacgatatga gtcgtctggc ggcctttctt tttctcaatg tatgagaggc
     6481 gcattggagt tctgctgttg atctcattaa cacagacctg caggaagcgg cggcggaagt
     6541 caggcatacg ctggtaactt tgaggcagct ggtaacgctc tatgatccag tcgattttca
     6601 gagagacgat gcctgagcca tccggcttac gatactgaca cagggattcg tataaacgca
     6661 tggcatacgg attggtgatt tcttttgttt cactaagccg aaactgcgta aaccggttct
     6721 gtaacccgat aaagaaggga atgagatatg ggttgatatg tacactgtaa agccctctgg
     6781 atggactgtg cgcacgtttg ataaaccaag gaaaagattc atagcctttt tcatcgccgg
     6841 catcctcttc agggcgataa aaaaccactt ccttccccgc gaaactcttc aatgcctgcc
     6901 gtatatcctt actggcttcc gcagaggtca atccgaatat ttcagcatat ttagcaacat
     6961 ggatctcgca gataccgtca tgttcctgta gggtgccatc agattttctg atctggtcaa
     7021 cgaacagata cagcatacgt ttttgatccc gggagagact atatgccgcc tcagtgaggt
     7081 cgtttgactg gacgattcgc gggctatttt tacgtttctt gtgattgata accgctgttt
     7141 ccgccatgac agatccatgt gaagtgtgac aagtttttag attgtcacac taaataaaaa
     7201 agagtcaata agcagggata actttgtgaa aaaacagctt cttctgaggg caatttgtca
     7261 cagggttaag ggcaatttgt cacagacagg actgtcattt gagggtgatt tgtcacactg
     7321 aaagggcaat ttgtcacaac accttctcta gaaccagcat ggataaaggc ctacaaggcg
     7381 ctctaaaaaa gaagatctaa aaactataaa aaaaataatt ataaaaatat ccccgtggat
     7441 aagtggataa ccccaaggga agttttttca ggcatcgtgt gtaagcagaa tatataagtg
     7501 ctgttccctg gtgcttcctc gctcactcga ccgggagggt tcgagaaggg ggggcacccc
     7561 ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa
     7621 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
     7681 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
     7741 ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
     7801 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
     7861 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
     7921 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
     7981 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc
     8041 cacaacgccg gcggccggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg
     8101 tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccg agggcttcgc
     8161 cctgtcgctc gactgcggcg agcactactg gctgtaaaag gacagaccac atcatggttc
     8221 tgtgttcatt aggttgttct gtccattgct gacataatcc gctccacttc aacgtaacac
     8281 cgcacgaaga tttctattgt tcctgaaggc atattcaaat cgttttcgtt accgcttgca
     8341 ggcatcatga cagaacacta cttcctataa acgctacaca ggctcctgag attaataatg
     8401 cggatctcta cgataatggg agattttccc gactgtttcg ttcgcttctc agtggataac
     8461 agccagcttc tctgtttaac agacaaaaac agcatatcca ctcagttcca catttccata
     8521 taaaggccaa ggcatttatt ctcaggataa ttgtttcagc atcgcaaccg catcagactc
     8581 cggcatcgca aactgcaccc ggtgccgggc agccacatcc agcgcaaaaa ccttcgtgta
     8641 gacttccgtt gaactgatgg acttatgtcc catcaggctt tgcagaactt tcagcggtat
     8701 accggcatac agcatgtgca tcgcatagga atggcggaac gtatgtggtg tgaccggaac
     8761 agagaacgtc acaccgtcag cagcagcggc ggcaaccgcc tccccaatcc aggtcctgac
     8821 cgttctgtcc gtcacttccc agatccgcgc tttctctgtc cttcctgtgc gacggttacg
     8881 ccgctccatg agcttatcgc gaataaatac ctgtgacgga agatcacttc gcagaataaa
     8941 taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag
     9001 acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
     9061 gcgtattttt tgagttatcg agattttcag gagctaa
//
