LOCUS       pTfdc                   5695 bp    DNA     circular SYN 11-DEC-2013
DEFINITION  Cloning vector pTrc99A, complete sequence.
ACCESSION   M22744
VERSION     M22744.1  GI:340740
KEYWORDS    cloning vector.
SOURCE      Cloning vector pTrc99A.
  ORGANISM  Cloning vector pTrc99A
            other sequences; artificial sequences; vectors.
REFERENCE   1  (sites)
  AUTHORS   Amann,E. and Brosius,J.
  TITLE     'ATG vectors' for regulated high-level expression of cloned genes
            in Escherichia coli
  JOURNAL   Gene 40 (2-3), 183-190 (1985)
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Amann,E., Ochs,B. and Abel,K.J.
  TITLE     Tightly regulated tac promoter vectors useful for the expression of
            unfused and fused proteins in Escherichia coli
  JOURNAL   Gene 69 (2), 301-315 (1988)
COMMENT     E. coli expression vector
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|681145254|
COMMENT     VNTDBDATE|681145254|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTfdc|
COMMENT     VNTAUTHORNAME|DN|
COMMENT     VNTOAUTHORNAME|Kristala Jones Prather|
COMMENT     VNTOAUTHORTEL|617-253-1950|
COMMENT     VNTOAUTHOREML|kljp@mit.edu|
COMMENT     VNTOAUTHORWWW|web.mit.edu/prathergroup|
COMMENT     VNTOAUTHORAD1|MIT Dept of Chemical Engineering|
COMMENT     VNTOAUTHORAD2|77 Massachusetts Avenue|
COMMENT     VNTOAUTHORAD3|Room 66-458|
COMMENT     VNTOAUTHORAD4|Cambridge, MA  02139|
FEATURES             Location/Qualifiers
     promoter        18..263
                     /citation=[1]
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="trc promoter from pKK233-2"
     misc_feature    1848..2272
                     /vntifkey="21"
                     /label=5S\rRNA,\T1,\T2,\rrnB
                     /note="5S RNA, T1, T2, rrnB"
     misc_feature    2273..4451
                     /vntifkey="21"
                     /label=derived\from\pBR322
                     /note="derived from cloning vector pBR322"
     misc_feature    4496..5682
                     /vntifkey="21"
                     /label=lacIq\region
                     /note="lacI-q region"
     CDS             2365..3225
                     /vntifkey="4"
                     /label=Amp-R
     promoter        4508..4544
                     /vntifkey="30"
                     /label=lacI-q\promoter
     CDS             4565..5656
                     /vntifkey="4"
                     /label=lacI
     CDS             328..1839
                     /vntifkey="4"
                     /label=FDC1
BASE COUNT     1473 a      1333 c      1456 g      1433 t 
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 
      241 taacaatttc acacaggaaa cagaccatgg aattcgagct cggtacccgg ggatcctcta 
      301 gagtcgacct gcagggggag gaattatatg aggaagctaa atccagcttt agaatttaga 
      361 gactttatcc aggtcttaaa agatgaagat gacttaatcg aaattaccga agagattgat 
      421 ccaaatctcg aagtaggtgc aattatgagg aaggcctatg aatcccactt accagccccg 
      481 ttatttaaaa atctcaaagg tgcttcgaag gatcttttca gcattttagg ttgcccagcc 
      541 ggtttgagaa gtaaggagaa aggagatcat ggtagaattg cccatcatct ggggctcgac 
      601 ccaaaaacaa ctatcaagga aatcatagat tatttgctgg agtgtaagga gaaggaacct 
      661 ctccccccaa tcactgttcc tgtgtcatct gcaccttgta aaacacatat actttctgaa 
      721 gaaaaaatac atctacaaag cctgccaaca ccatatctac atgtttcaga cggtggcaag 
      781 tacttacaaa cgtacggaat gtggattctt caaactccag ataaaaaatg gactaattgg 
      841 tcaattgcta gaggtatggt tgtagatgac aagcatatca ctggtctggt aattaaacca 
      901 caacatatta gacaaattgc tgactcttgg gcagcaattg gaaaagcaaa tgaaattcct 
      961 ttcgcgttat gttttggcgt tcccccagca gctattttag ttagttccat gccaattcct 
     1021 gaaggtgttt ctgaatcgga ttatgttggc gcaatcttgg gtgagtcggt tccagtagta 
     1081 aaatgtgaga ccaacgattt aatggttcct gcaacgagtg agatggtatt tgagggtact 
     1141 ttgtccttaa cagatacaca tctggaaggc ccatttggtg agatgcatgg atatgttttc 
     1201 aaaagccaag gtcatccttg tccattgtac actgtcaagg ctatgagtta cagagacaat 
     1261 gctattctac ctgtttcgaa ccccggtctt tgtacggatg agacacatac cttgattggt 
     1321 tcactagtgg ctactgaggc caaggagctg gctattgaat ctggcttgcc aattctggat 
     1381 gcctttatgc cttatgaggc tcaggctctt tggcttatct taaaggtgga tttgaaaggg 
     1441 ctgcaagcat tgaagacaac gcctgaagaa ttttgtaaga aggtaggtga tatttacttt 
     1501 aggacaaaag ttggttttat agtccatgaa ataattttgg tggcagatga tatcgacata 
     1561 tttaacttca aagaagtcat ctgggcctac gttacaagac atacacctgt tgcagatcag 
     1621 atggcttttg atgatgtcac ttcttttcct ttggctccct ttgtttcgca gtcatccaga 
     1681 agtaagacta tgaaaggtgg aaagtgcgtt actaattgca tatttagaca gcaatatgag 
     1741 cgcagttttg actacataac ttgtaatttt gaaaagggat atccaaaagg attagttgac 
     1801 aaagtaaatg aaaattggaa aaggtacgga tataaataaa agcttggctg ttttggcgga 
     1861 tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa 
     1921 cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa 
     1981 gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc 
     2041 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 
     2101 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc 
     2161 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat 
     2221 taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc tttttgttta 
     2281 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 
     2341 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 
     2401 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 
     2461 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 
     2521 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 
     2581 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc 
     2641 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 
     2701 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 
     2761 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 
     2821 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 
     2881 aacgacgagc gtgacaccac gatgcctaca gcaatggcaa caacgttgcg caaactatta 
     2941 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 
     3001 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 
     3061 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 
     3121 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 
     3181 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 
     3241 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 
     3301 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 
     3361 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
     3421 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
     3481 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
     3541 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
     3601 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
     3661 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
     3721 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
     3781 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
     3841 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
     3901 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
     3961 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
     4021 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 
     4081 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 
     4141 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg 
     4201 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag 
     4261 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc 
     4321 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 
     4381 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 
     4441 gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg gcatgcattt acgttgacac 
     4501 catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt 
     4561 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc 
     4621 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga 
     4681 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact 
     4741 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc 
     4801 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt 
     4861 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc 
     4921 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt 
     4981 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat 
     5041 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc 
     5101 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct 
     5161 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg 
     5221 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg 
     5281 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc 
     5341 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac 
     5401 cgaagacagc tcatgttata tcccgccgtc aaccaccatc aaacaggatt ttcgcctgct 
     5461 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa 
     5521 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac 
     5581 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 
     5641 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gcgcgaattg atctg 
//
