LOCUS       pTpal-fdc               7837 bp    DNA     circular SYN 11-DEC-2013
DEFINITION  Cloning vector pTrc99A, complete sequence.
ACCESSION   M22744
VERSION     M22744.1  GI:340740
KEYWORDS    cloning vector.
SOURCE      Cloning vector pTrc99A.
  ORGANISM  Cloning vector pTrc99A
            other sequences; artificial sequences; vectors.
REFERENCE   1  (sites)
  AUTHORS   Amann,E. and Brosius,J.
  TITLE     'ATG vectors' for regulated high-level expression of cloned genes
            in Escherichia coli
  JOURNAL   Gene 40 (2-3), 183-190 (1985)
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Amann,E., Ochs,B. and Abel,K.J.
  TITLE     Tightly regulated tac promoter vectors useful for the expression of
            unfused and fused proteins in Escherichia coli
  JOURNAL   Gene 69 (2), 301-315 (1988)
COMMENT     E. coli expression vector
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|681142371|
COMMENT     VNTDBDATE|681142371|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTpal-fdc|
COMMENT     VNTAUTHORNAME|DN|
COMMENT     VNTOAUTHORNAME|Kristala Jones Prather|
COMMENT     VNTOAUTHORTEL|617-253-1950|
COMMENT     VNTOAUTHOREML|kljp@mit.edu|
COMMENT     VNTOAUTHORWWW|web.mit.edu/prathergroup|
COMMENT     VNTOAUTHORAD1|MIT Dept of Chemical Engineering|
COMMENT     VNTOAUTHORAD2|77 Massachusetts Avenue|
COMMENT     VNTOAUTHORAD3|Room 66-458|
COMMENT     VNTOAUTHORAD4|Cambridge, MA  02139|
FEATURES             Location/Qualifiers
     promoter        18..263
                     /citation=[1]
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="trc promoter from pKK233-2"
     misc_feature    3990..4414
                     /vntifkey="21"
                     /label=5S\rRNA,\T1,\T2,\rrnB
                     /note="5S RNA, T1, T2, rrnB"
     misc_feature    4415..6593
                     /vntifkey="21"
                     /label=derived\from\pBR322
                     /note="derived from cloning vector pBR322"
     misc_feature    6638..7824
                     /vntifkey="21"
                     /label=lacIq\region
                     /note="lacI-q region"
     CDS             4507..5367
                     /vntifkey="4"
                     /label=Amp-R
     promoter        6650..6686
                     /vntifkey="30"
                     /label=lacI-q\promoter
     CDS             6707..7798
                     /vntifkey="4"
                     /label=lacI
     CDS             285..2438
                     /vntifkey="4"
                     /label=PAL2
     CDS             2470..3981
                     /vntifkey="4"
                     /label=FDC1
BASE COUNT     2076 a      1777 c      2004 g      1980 t 
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 
      241 taacaatttc acacaggaaa cagaccatgg gcgggaggta accaatggat caaatcgaag 
      301 caatgttgtg cggcggagga gagaagacaa aagtggcggt tactacgaag actttggcag 
      361 atccattgaa ttggggttta gcagcggatc aaatgaaagg aagtcattta gatgaagtga 
      421 agaagatggt cgaagagtat cgtagaccag tcgtgaatct tggcggagaa acactgacga 
      481 tcggacaagt tgctgccatc tccaccgtag gaggcagcgt taaggttgag ttagcggaga 
      541 cttcaagagc cggtgtgaaa gctagcagtg attgggttat ggagagcatg aacaaaggta 
      601 ctgacagtta cggagtcacc accggctttg gtgctacttc tcaccggaga accaaaaacg 
      661 gcaccgcatt acaaacagaa ctcattagat ttttgaacgc cggaatattc ggaaacacga 
      721 aggagacatg tcacacactg ccgcaatccg ccacaagagc cgccatgctc gtcagagtca 
      781 acactcttct ccaaggatac tccgggatcc gattcgagat cctcgaagcg attacaagtc 
      841 tcctcaacca caacatctct ccgtcactac ctctccgtgg aaccattacc gcctccggcg 
      901 atctcgttcc tctctcttac atcgccggac ttctcaccgg ccgtcctaat tccaaagcca 
      961 ccggtcccga cggtgaatcg ctaaccgcga aagaagcttt tgagaaagcc ggaatcagta 
     1021 ctggattctt cgatttacaa cctaaggaag gtttagctct cgttaatggc acggcggttg 
     1081 gatctggaat ggcgtcgatg gttctattcg aagcgaatgt ccaagcggtg ttagcggagg 
     1141 ttttatcagc gatcttcgcg gaggttatga gcgggaaacc tgagtttacc gatcatctga 
     1201 ctcatcgttt aaaacatcat cccggacaaa tcgaagcggc ggcgataatg gagcacatac 
     1261 tcgacggaag ctcatacatg aaattagctc aaaaggttca cgagatggat ccattgcaga 
     1321 aaccaaaaca agatcgttac gctcttcgta catctcctca atggctaggt cctcaaattg 
     1381 aagtaatccg tcaagctacg aaatcgatag agcgtgaaat caactccgtt aacgataatc 
     1441 cgttgatcga tgtttcgagg aacaaggcga ttcacggtgg taacttccaa ggaacaccaa 
     1501 tcggagtttc tatggataac acgagattgg cgattgctgc gattgggaag ctaatgtttg 
     1561 ctcaattctc tgagcttgtt aatgatttct acaacaatgg acttccttcg aatctaactg 
     1621 cttcgagtaa tccaagtttg gattatggat tcaaaggagc agagattgct atggcttctt 
     1681 attgttctga gcttcaatac ttggctaatc cagtcacaag ccatgttcaa tcagctgagc 
     1741 aacataatca agatgtgaac tctcttggtt tgatctcgtc tcgtaaaaca tctgaagctg 
     1801 tggatattct taagctaatg tcaacaacgt tccttgtggg gatatgtcaa gctgttgatt 
     1861 tgagacattt ggaggagaat ctgagacaaa ctgtgaagaa cacagtttct caagttgcta 
     1921 agaaagtgtt aaccactgga atcaacggtg agttacatcc gtcaaggttt tgcgagaagg 
     1981 acttgcttaa ggttgttgat cgtgagcaag tgttcacgta tgtggatgat ccttgtagcg 
     2041 ctacgtaccc gttgatgcag agactaagac aagttattgt tgatcacgct ttgtccaacg 
     2101 gtgagactga gaagaatgca gtgacttcga tctttcaaaa gattggagct tttgaagagg 
     2161 agcttaaggc tgtgcttcca aaggaagttg aagcggctag agcggcttat gggaatggaa 
     2221 ctgcgccgat tcctaaccgg attaaggaat gtaggtcgta tccgttgtat aggttcgtga 
     2281 gggaagagct tggaacgaag ttgttgactg gagaaaaggt tgtgtctccg ggagaggagt 
     2341 ttgataaggt cttcactgct atgtgtgaag gtaaacttat tgatccgttg atggattgtc 
     2401 tcaaggaatg gaacggagct ccgattccga tttgctaatc tagagtcgac ctgcaggggg 
     2461 aggaattata tgaggaagct aaatccagct ttagaattta gagactttat ccaggtctta 
     2521 aaagatgaag atgacttaat cgaaattacc gaagagattg atccaaatct cgaagtaggt 
     2581 gcaattatga ggaaggccta tgaatcccac ttaccagccc cgttatttaa aaatctcaaa 
     2641 ggtgcttcga aggatctttt cagcatttta ggttgcccag ccggtttgag aagtaaggag 
     2701 aaaggagatc atggtagaat tgcccatcat ctggggctcg acccaaaaac aactatcaag 
     2761 gaaatcatag attatttgct ggagtgtaag gagaaggaac ctctcccccc aatcactgtt 
     2821 cctgtgtcat ctgcaccttg taaaacacat atactttctg aagaaaaaat acatctacaa 
     2881 agcctgccaa caccatatct acatgtttca gacggtggca agtacttaca aacgtacgga 
     2941 atgtggattc ttcaaactcc agataaaaaa tggactaatt ggtcaattgc tagaggtatg 
     3001 gttgtagatg acaagcatat cactggtctg gtaattaaac cacaacatat tagacaaatt 
     3061 gctgactctt gggcagcaat tggaaaagca aatgaaattc ctttcgcgtt atgttttggc 
     3121 gttcccccag cagctatttt agttagttcc atgccaattc ctgaaggtgt ttctgaatcg 
     3181 gattatgttg gcgcaatctt gggtgagtcg gttccagtag taaaatgtga gaccaacgat 
     3241 ttaatggttc ctgcaacgag tgagatggta tttgagggta ctttgtcctt aacagataca 
     3301 catctggaag gcccatttgg tgagatgcat ggatatgttt tcaaaagcca aggtcatcct 
     3361 tgtccattgt acactgtcaa ggctatgagt tacagagaca atgctattct acctgtttcg 
     3421 aaccccggtc tttgtacgga tgagacacat accttgattg gttcactagt ggctactgag 
     3481 gccaaggagc tggctattga atctggcttg ccaattctgg atgcctttat gccttatgag 
     3541 gctcaggctc tttggcttat cttaaaggtg gatttgaaag ggctgcaagc attgaagaca 
     3601 acgcctgaag aattttgtaa gaaggtaggt gatatttact ttaggacaaa agttggtttt 
     3661 atagtccatg aaataatttt ggtggcagat gatatcgaca tatttaactt caaagaagtc 
     3721 atctgggcct acgttacaag acatacacct gttgcagatc agatggcttt tgatgatgtc 
     3781 acttcttttc ctttggctcc ctttgtttcg cagtcatcca gaagtaagac tatgaaaggt 
     3841 ggaaagtgcg ttactaattg catatttaga cagcaatatg agcgcagttt tgactacata 
     3901 acttgtaatt ttgaaaaggg atatccaaaa ggattagttg acaaagtaaa tgaaaattgg 
     3961 aaaaggtacg gatataaata aaagcttggc tgttttggcg gatgagagaa gattttcagc 
     4021 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc 
     4081 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc 
     4141 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg 
     4201 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 
     4261 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg 
     4321 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct 
     4381 gacggatggc ctttttgcgt ttctacaaac tctttttgtt tatttttcta aatacattca 
     4441 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 
     4501 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 
     4561 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 
     4621 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 
     4681 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 
     4741 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 
     4801 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 
     4861 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 
     4921 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 
     4981 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 
     5041 acgatgccta cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 
     5101 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 
     5161 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 
     5221 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 
     5281 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 
     5341 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 
     5401 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 
     5461 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 
     5521 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 
     5581 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 
     5641 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg 
     5701 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 
     5761 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 
     5821 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 
     5881 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 
     5941 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 
     6001 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 
     6061 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 
     6121 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 
     6181 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 
     6241 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 
     6301 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc 
     6361 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc 
     6421 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg 
     6481 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 
     6541 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcagatca 
     6601 attcgcgcgc gaaggcgaag cggcatgcat ttacgttgac accatcgaat ggtgcaaaac 
     6661 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa 
     6721 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg 
     6781 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat 
     6841 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt 
     6901 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc 
     6961 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag 
     7021 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct 
     7081 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa 
     7141 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc 
     7201 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat 
     7261 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca 
     7321 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc 
     7381 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat 
     7441 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct 
     7501 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta 
     7561 tatcccgccg tcaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga 
     7621 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc 
     7681 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt 
     7741 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 
     7801 gcaacgcaat taatgtgagt tagcgcgaat tgatctg 
//
