LOCUS       Exported                4331 bp ds-DNA     circular SYN 14-DEC-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 14, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        162..1449
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1451..1504
                     /label=MCS
                     /note="color: #a6acb3"
     misc_feature    1509..1590
                     /label=pA SS
                     /note="color: #a6acb3"
     repeat_region   1594..1734
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      1809..2264
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        2546..2650
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2651..3511
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2651 .. 2719 / #ccffcc / signal sequence
                        2: 2720 .. 3511 / #ccffcc
                     Cleavage site after base 2719"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3682..4270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttcgta acattatggc cttaggtcac
      181 ttcatctcca tggggttctt cttctgattt tctagaaaat gagatggggg tgcagagagc
      241 ttcctcagtg acctgcccag ggtcacatca gaaatgtcag agctagaact tgaactcaga
      301 ttactaatct taaattccat gccttggggg catgcaagta cgatatacag aaggagtgaa
      361 ctcattaggg cagatgacca atgagtttag gaaagaagag tccagggcag ggtacatcta
      421 caccacccgc ccagccctgg gtgagtccag ccacgttcac ctcattatag ttgcctctct
      481 ccagtcctac cttgacggga agcacaagca gaaactggga caggagcccc aggagaccaa
      541 atcttcatgg tccctctggg aggatgggtg gggagagctg tggcagaggc ctcaggaggg
      601 gccctgctgc tcagtggtga cagatagggg tgagaaagca gacagagtca ttccgtcagc
      661 attctgggtc tgtttggtac ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg
      721 ctaaaaagca gggagagctg gaaagaaaca aggacagaga cagaggccaa gtcaaccaga
      781 ccaattccca gaggaagcaa agaaaccatt acagagacta caagggggaa gggaaggaga
      841 gatgaattag cttcccctgt aaaccttaga acccagctgt tgccagggca acggggcaat
      901 acctgtctct tcagaggaga tgaagttgcc agggtaacta catcctgtct ttctcaagga
      961 ccatcccaga atgtggcacc cactagccgt taccatagca actgcctctt tgccccactt
     1021 aatcccatcc cgtctgttaa aagggcccta tagttggagg tgggggaggt aggaagagcg
     1081 atgatcactt gtggactaag tttgttcgca tccccttctc caaccccctc agtacatcac
     1141 cctgggggaa cagggtccac ttgctcctgg gcccacacag tcctgcagta ttgtgtatat
     1201 aaggccaggg caaagaggag caggttttaa agtgaaaggc aggcaggtgt tggggaggca
     1261 gttaccgggg caacgggaac agggcgtttc ggaggtggtt gccatgggga cctggatgct
     1321 gacgaaggct cgcgaggctg tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt
     1381 cagtcaagcc ggttctccgt ttgcactcag gagcacgggc aggcgagtgg cccctagttc
     1441 tgggagcagg aattcaagct tgctcagcca tatggatatc gcatgcggat ccactagtct
     1501 cgagtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1561 taaagcattt ttttcactgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     1621 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     1681 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     1741 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     1801 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1861 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1921 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     1981 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     2041 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2101 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     2161 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2221 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     2281 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     2341 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2401 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     2461 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2521 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2581 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2641 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2701 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2761 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2821 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2881 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2941 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3001 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3061 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3121 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3181 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3241 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3301 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3361 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3421 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3481 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3541 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3601 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3661 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3721 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3781 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3841 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3901 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3961 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4021 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4081 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4141 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4201 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4261 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4321 tgctcacatg t
//
