LOCUS       pExchange1-pEF-H        9156 bp    DNA     circular     18-FEB-2016
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11113474|
COMMENT     VNTDBDATE|-11113480|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pExchange1-pEF-H2B-mcherry-T2A-rTetR-HDAC4.gb|
COMMENT     VNTAUTHORNAME|Lacra|
COMMENT     VNTOAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pExchange1-pEF-H2B-mcherry-T2A-rTetR-HDAC4.gb" 0 9156 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList) 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 1620 1661 1 9156 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "SV40 pA" 21 0 0 6365 6748 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "811..1194") 
COMMENT        #1=(CFSignal (CObList) "f1 ori" 21 0 0 6886 7192 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1332..1638") 
COMMENT        #2=(CFSignal (CObList) "loxP" 21 0 0 7350 7384 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1796..1830") 
COMMENT        #3=(CFSignal (CObList) "AMP" 21 0 0 7433 8290 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1879..2736") 
COMMENT        #4=(CFSignal (CObList) "pUC ori" 21 0 0 8437 9093 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2883..3539") 
COMMENT        #5=(CFSignal (CObList) "T3 Primer" 28 0 0 1185 1204 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "T7 Primer" 27 0 0 6332 6353 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "PEF-1alpha" 29 0 0 7 1161 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "H2B" 4 0 0 1264 1641 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2544..2921") 
COMMENT        #9=(CFSignal (CObList) "mCherry" 4 0 0 1648 2358 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2928..3638") 
COMMENT        #10=(CFSignal (CObList) "T2A" 21 0 0 2359 2412 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #11=(CFSignal (CObList) "rTetR" 21 0 0 2413 3030 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "LINKER" 21 0 0 3031 3054 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "Kozak" 21 0 0 1258 1263 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "HDAC4" 21 0 0 3055 6303 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "silent mutation c->t" 21 0 0 5616 5616 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "silent c->t" 21 0 0 1899 1899 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #17=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #18=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "pExchange1-pEF-H2B-mcherry-T2A-rTetR-HDAC4.gb" 2) 5 "" 0 4) 
COMMENT              #19=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pExchange1-pEF-H2B-mcherry-T2A-rTetR-HDAC4.gb'" 
COMMENT                          1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: Demo User" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/18/16 02:49PM" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/18/16 02:55PM" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9156 bp" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #27=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 22-APR-2008" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #30=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Demo User" 
COMMENT                          1))) "Original Author") 
COMMENT              #32=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #33=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #36=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1264 (3 #8# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #37=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1264  End: 1641" 1) 
COMMENT                                   #38=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #39=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2544..2921" 1))) "H2B") 
COMMENT                            #40=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1648 (3 #9# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1648  End: 2358" 1) 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2928..3638" 1))) "mCherry"))) 
COMMENT                          "CDS (2 total)") 
COMMENT                     #44=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #45=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1258 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1258  End: 1263" 1))) "Kozak") 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1899 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1899  End: 1899" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "silent mutation in all the lab mcherry constructs" 
COMMENT                                        1))) "silent c->t") 
COMMENT                            #50=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2359 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2359  End: 2412" 1))) "T2A") 
COMMENT                            #52=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2413 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2413  End: 3030" 1))) "rTetR") 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3031 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3031  End: 3054" 1))) "LINKER") 
COMMENT                            #56=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3055 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3055  End: 6303" 1))) "HDAC4") 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5616 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5616  End: 5616" 1))) 
COMMENT                                 "silent mutation c->t") 
COMMENT                            #60=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6365 (3 #0# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6365  End: 6748" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   811..1194" 1))) "SV40 pA") 
COMMENT                            #64=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6886 (3 #1# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6886  End: 7192" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1332..1638" 1))) "f1 ori") 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7350 (3 #2# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7350  End: 7384" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1796..1830" 1))) "loxP") 
COMMENT                            #72=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7433 (3 #3# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7433  End: 8290" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1879..2736" 1))) "AMP") 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 8437 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 8437  End: 9093" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2883..3539" 1))) "pUC ori"))) 
COMMENT                          "Misc. Feature (12 total)") 
COMMENT                     #80=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #81=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6332 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6332  End: 6353" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "T7 Primer" 1))) "T7 Primer"))) 
COMMENT                          "Primer (1 total)") 
COMMENT                     #84=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 28 (7 28 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #85=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1185 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1185  End: 1204" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "T3 Primer" 1))) "T3 Primer"))) 
COMMENT                          "Primer Binding Site (1 total)") 
COMMENT                     #88=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #89=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 7 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7     End: 1161" 1))) 
COMMENT                                 "PEF-1alpha"))) "Promoter Eukaryotic (1 total)"))) 
COMMENT                   "Feature Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #91=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT               "@N (@S)" 0) 
COMMENT          #92=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT               "@N" 0) 
COMMENT          #93=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT               "@L bp" 0) 
COMMENT          #94=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT               "@N" 0) 
COMMENT          #95=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT               (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #96=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT               (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #97=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT               (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #98=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT               (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #99=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT               (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #100=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #101=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #102=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #103=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #104=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #105=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #106=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #107=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #108=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #109=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #110=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #111=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #112=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #113=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #114=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #115=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #116=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #117=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #118=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #119=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #120=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #121=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #122=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #123=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #124=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #125=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #126=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #127=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #128=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #129=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #130=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 
COMMENT         -4167) 1 1 0 1 1 
COMMENT        (mapper: 18.7927 -14.4718 40.0378 40.0378 0.01 10 -1 9156 9156 1 0 0) 
COMMENT        #131=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1818321775 100) 
COMMENT              (CObjectList 
COMMENT               #132=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -181 100) 
COMMENT                     (CObjectList 
COMMENT                      #133=(CAxis 
COMMENT                            (CWideLine (CWidget 0 (0 0) 1 2 0 0 #97# 93753324 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27487 6.27287 1 0.0214037) 0.0685145) 
COMMENT                      #134=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #94# 170 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 
COMMENT                            "pExchange1-pEF-H2B-mcherry-T2A-rTetR-HDAC4.gb" "@N" 
COMMENT                            1 0 0.871165 0 -10 30.9458 1.39868 Nil) 
COMMENT                      #135=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #93# 42579944 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "9156 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 2.52262 0.799245 Nil)) (CObjectList)) 
COMMENT               #136=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -251 100) 
COMMENT                     (CObjectList 
COMMENT                      #137=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -809 100) 
COMMENT                            (CObjectList 
COMMENT                             #138=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #95# 484 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 5.14886 5.40785 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #139=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "H2B" "@N" 1 
COMMENT                                   11.8977 5.22699 0 -10 1.87323 1.04901 #138#) 
COMMENT                             #140=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #95# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 4.65761 5.14475 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #141=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "mCherry" "@N" 1 
COMMENT                                   13.7726 1.74233 0 -10 3.72148 1.04901 #140#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #142=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -739 100) 
COMMENT                            (CObjectList 
COMMENT                             #143=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #0# 0) 1 2 0 0 #96# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 1.64983 1.91292 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #144=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #92# 3070776 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 pA" "@N" 1 
COMMENT                                   -13.6768 -1.74233 0 -10 3.79641 1.04901 #143#) 
COMMENT                             #145=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #96# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 1.34562 1.55596 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #146=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 ori" "@N" 1 
COMMENT                                   -13.0734 1.74233 0 -10 2.29783 1.04901 #145#) 
COMMENT                             #147=(CScratch 
COMMENT                                   (CWidget 0 (3 #2# 0) 1 2 0 0 #100# 1093 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 1.23806 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #148=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #92# -800 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 
COMMENT                                   -12.5307 3.48466 0 -10 2.04806 1.04901 #147#) 
COMMENT                             #149=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #96# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 0.593335 
COMMENT                                    1.18119 1 0.082322) 0.8 1.8 0) 
COMMENT                             #150=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #92# 7208984 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AMP" "@N" 1 
COMMENT                                   -11.0855 6.96932 0 -10 2.12299 1.04901 #149#) 
COMMENT                             #151=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #96# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 0.0431641 0.493304 
COMMENT                                    1 0.082322) 0.8 1.8 0) 
COMMENT                             #152=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #92# 94076208 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pUC ori" "@N" 1 
COMMENT                                   -5.59507 12.1963 0 -10 3.27191 1.04901 #151#) 
COMMENT                             #153=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 4.65761 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #154=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T2A" "@N" 1 
COMMENT                                   12.8556 0 0 -10 1.6984 1.04901 #153#) 
COMMENT                             #155=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #96# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 4.1972 
COMMENT                                    4.62062 1 0.082322) 0.8 1.8 0) 
COMMENT                             #156=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "rTetR" "@N" 1 
COMMENT                                   12.7746 -3.48466 0 -10 2.29783 1.04901 #155#) 
COMMENT                             #157=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 4.1972 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #158=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #92# 842239321 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "LINKER" "@N" 1 
COMMENT                                   12.6295 -5.22699 0 -10 3.37181 1.04901 #157#) 
COMMENT                             #159=(CScratch 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.41196 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #160=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Kozak" "@N" 1 
COMMENT                                   11.4253 6.96932 0 -10 2.7474 1.04901 #159#) 
COMMENT                             #161=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #96# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 1.95472 
COMMENT                                    4.18075 1 0.082322) 0.8 1.8 0) 
COMMENT                             #162=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 354 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "HDAC4" "@N" 1 
COMMENT                                   -3.18288 -12.1963 0 -10 3.09707 1.04901 #161#) 
COMMENT                             #163=(CScratch 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 2.4261 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #164=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "silent mutation c->t" "@N" 1 -12.8833 
COMMENT                                   -8.71165 0 -10 8.29216 1.04901 #163#) 
COMMENT                             #165=(CScratch 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #100# 1093 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 4.97278 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #166=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "silent c->t" 
COMMENT                                   "@N" 1 13.8125 3.48466 0 -10 4.32092 1.04901 
COMMENT                                   #165#)) (CObjectList)) 
COMMENT                      #167=(CGroupWidget 
COMMENT                            (CWidget 27 (7 27 0) 1 2 0 0 Nil -1088 100) 
COMMENT                            (CObjectList 
COMMENT                             #168=(CScratch 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 1.93553 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #169=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 8 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 Primer" "@N" 
COMMENT                                   1 -13.6632 -3.48466 0 -10 4.24599 1.04901 
COMMENT                                   #168#)) (CObjectList)) 
COMMENT                      #170=(CGroupWidget 
COMMENT                            (CWidget 28 (7 28 0) 1 2 0 0 Nil -949 100) 
COMMENT                            (CObjectList 
COMMENT                             #171=(CScratch 
COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #113# 43560448 
COMMENT                                    100) (LOGPEN 0 0 16711680) 1 1 5.46197 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #172=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 759 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T3 Primer" "@N" 
COMMENT                                   1 11.3672 8.71165 0 -10 4.24599 1.04901 #171#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #173=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -321 100) 
COMMENT                            (CObjectList 
COMMENT                             #174=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #96# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.47773 
COMMENT                                    6.26907 1 0.082322) 0.8 1.8 0) 
COMMENT                             #175=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #92# 1215260994 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "PEF-1alpha" 
COMMENT                                   "@N" 1 8.93019 10.454 0 -10 5.04523 1.04901 
COMMENT                                   #174#)) (CObjectList))) (CObjectList)) 
COMMENT               #176=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -739 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #177=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -600 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #178=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -112 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 93841024 
COMMENT       (CStringList) 93841384 93877632 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    6365..6748
                     /vntifkey="21"
                     /label=SV40\pA
     misc_feature    6886..7192
                     /vntifkey="21"
                     /label=f1\ori
     misc_feature    7350..7384
                     /vntifkey="21"
                     /label=loxP
     misc_feature    7433..8290
                     /vntifkey="21"
                     /label=AMP
     misc_feature    8437..9093
                     /vntifkey="21"
                     /label=pUC\ori
     primer_bind     1185..1204
                     /vntifkey="28"
                     /label=T3\Primer
                     /note="T3 Primer"
     primer          6332..6353
                     /vntifkey="27"
                     /label=T7\Primer
                     /note="T7 Primer"
     promoter        7..1161
                     /vntifkey="29"
                     /label=PEF-1alpha
     CDS             1264..1641
                     /vntifkey="4"
                     /label=H2B
     CDS             1648..2358
                     /vntifkey="4"
                     /label=mCherry
     misc_feature    2359..2412
                     /vntifkey="21"
                     /label=T2A
     misc_feature    2413..3030
                     /vntifkey="21"
                     /label=rTetR
     misc_feature    3031..3054
                     /vntifkey="21"
                     /label=LINKER
     misc_feature    1258..1263
                     /vntifkey="21"
                     /label=Kozak
     misc_feature    3055..6303
                     /vntifkey="21"
                     /label=HDAC4
     misc_feature    5616..5616
                     /vntifkey="21"
                     /label=silent\mutation\c->t
     misc_feature    1899..1899
                     /vntifkey="21"
                     /label=silent\c->t
                     /note="silent mutation in all the lab mcherry constructs"
BASE COUNT     2100 a      2628 c      2587 g      1841 t 
ORIGIN
        1 atgcatgagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 
       61 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 
      121 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 
      181 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg 
      241 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag 
      301 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc 
      361 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc 
      421 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc 
      481 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat 
      541 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc 
      601 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc 
      661 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt 
      721 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga 
      781 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag 
      841 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca 
      901 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg 
      961 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag 
     1021 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc 
     1081 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt 
     1141 cttccatttc aggtgtcgtg agctagcgat tacgccaagc tcgaaattaa ccctcactaa 
     1201 agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagcccggg cggatccgcc 
     1261 accatgccag agccagcgaa gtctgctccc gccccgaaaa agggctccaa gaaggcggtg 
     1321 actaaggcgc agaagaaagg cggcaagaag cgcaagcgca gccgcaagga gagctattcc 
     1381 atctatgtgt acaaggttct gaagcaggtc caccctgaca ccggcatttc gtccaaggcc 
     1441 atgggcatca tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc 
     1501 cgcctggcgc attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg 
     1561 cgcctgctgc tgcctgggga gttggccaag cacgccgtgt ccgagggtac taaggccatc 
     1621 accaagtaca ccagcgctaa gcctaggatg gtgagcaagg gcgaggagga taacatggcc 
     1681 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 
     1741 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 
     1801 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 
     1861 tacggctcca aggcctacgt gaagcacccc gccgacattc ccgactactt gaagctgtcc 
     1921 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 
     1981 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 
     2041 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 
     2101 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 
     2161 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 
     2221 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 
     2281 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 
     2341 gacgagctgt acaagtctga gggcaggggg agcctcctaa cttgcggaga cgtcgaggag 
     2401 aatcctggcc catctagact ggacaagagc aaagtcataa acggagctct ggaattactc 
     2461 aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag 
     2521 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc 
     2581 gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac 
     2641 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct 
     2701 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc 
     2761 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg 
     2821 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa 
     2881 agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc 
     2941 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg 
     3001 gagaaacagc taaagtgcga aagcggcggg ggtagcggca gcggtagcgc atgcagctcc 
     3061 caaagccatc cagatggact ttctggccga gaccagccag tggagctgct gaatcctgcc 
     3121 cgcgtgaacc acatgcccag cacggtggat gtggccacgg cgctgcctct gcaagtggcc 
     3181 ccctcggcag tgcccatgga cctgcgcctg gaccaccagt tctcactgcc tgtggcagag 
     3241 ccggccctgc gggagcagca gctgcagcag gagctcctgg cgctcaagca gaagcagcag 
     3301 atccagaggc agatcctcat cgctgagttc cagaggcagc acgagcagct ctcccggcag 
     3361 cacgaggcgc agctccacga gcacatcaag caacaacagg agatgctggc catgaagcac 
     3421 cagcaggagc tgctggaaca ccagcggaag ctggagaggc accgccagga gcaggagctg 
     3481 gagaagcagc accgggagca gaagctgcag cagctcaaga acaaggagaa gggcaaagag 
     3541 agtgccgtgg ccagcacaga agtgaagatg aagttacaag aatttgtcct caataaaaag 
     3601 aaggcgctgg cccaccggaa tctgaaccac tgcatttcca gcgaccctcg ctactggtac 
     3661 gggaaaacgc agcacagttc ccttgaccag agttctccac cccagagcgg agtgtcgacc 
     3721 tcctataacc acccggtcct gggaatgtac gacgccaaag atgacttccc tcttaggaaa 
     3781 acagcttctg aaccgaatct gaaattacgg tccaggctaa agcagaaagt ggccgaaaga 
     3841 cggagcagcc ccctgttacg caggaaagac gggccagtgg tcactgctct aaaaaagcgt 
     3901 ccgttggatg tcacagactc cgcgtgcagc agcgccccag gctccggacc cagctcaccc 
     3961 aacaacagct ccgggagcgt cagcgcggag aacggtatcg cgcccgccgt ccccagcatc 
     4021 ccggcggaga cgagtttggc gcacagactt gtggcacgag aaggctcggc cgctccactt 
     4081 cccctctaca catcgccatc cttgcccaac atcacgctgg gcctgcctgc caccggcccc 
     4141 tctgcgggca cggcgggcca gcaggacgcc gagagactca cccttcccgc cctccagcag 
     4201 aggctctccc ttttccccgg cacccacctc actccctacc tgagcacctc gcccttggag 
     4261 cgggacggag gggcagcgca cagccctctt ctgcagcaca tggtcttact ggagcagccg 
     4321 ccggcacaag cacccctcgt cacaggcctg ggagcactgc ccctccacgc acagtccttg 
     4381 gttggtgcag accgggtgtc cccctccatc cacaagctgc ggcagcaccg cccactgggg 
     4441 cggacccagt cggccccgct gccccagaac gcccaggctc tgcagcacct ggtcatccag 
     4501 cagcagcatc agcagtttct ggagaaacac aagcagcagt tccagcagca gcaactgcag 
     4561 atgaacaaga tcatccccaa gccaagcgag ccagcccggc agccggagag ccacccggag 
     4621 gagacggagg aggagctccg tgagcaccag gctctgctgg acgagcccta cctggaccgg 
     4681 ctgccggggc agaaggaggc gcacgcacag gccggcgtgc aggtgaagca ggagcccatt 
     4741 gagagcgatg aggaagaggc agagccccca cgggaggtgg agccgggcca gcgccagccc 
     4801 agtgagcagg agctgctctt cagacagcaa gccctcctgc tggagcagca gcggatccac 
     4861 cagctgagga actaccaggc gtccatggag gccgccggca tccccgtgtc cttcggcggc 
     4921 cacaggcctc tgtcccgggc gcagtcctca cccgcgtctg ccaccttccc cgtgtctgtg 
     4981 caggagcccc ccaccaagcc gaggttcacg acaggcctcg tgtatgacac gctgatgctg 
     5041 aagcaccagt gcacctgcgg gagtagcagc agccaccccg agcacgccgg gaggatccag 
     5101 agcatctggt cccgcctgca ggagacgggc ctccggggca aatgcgagtg catccgcgga 
     5161 cgcaaggcca ccctggagga gctacagacg gtgcactcgg aagcccacac cctcctgtat 
     5221 ggcacgaacc ccctcaaccg gcagaaactg gacagtaaga aacttctagg ctcgctcgcc 
     5281 tccgtgttcg tccggctccc ttgcggtggt gttggggtgg acagtgacac catatggaac 
     5341 gaggtgcact cggcgggggc agcccgcctg gctgtgggct gcgtggtaga gctggtcttc 
     5401 aaggtggcca caggggagct gaagaatggc tttgctgtgg tccgcccccc tggacaccat 
     5461 gcggaggaga gcacgcccat gggcttttgc tacttcaact ccgtggccgt ggcagccaag 
     5521 cttctgcagc agaggttgag cgtgagcaag atcctcatcg tggactggga cgtgcaccat 
     5581 ggaaacggga cccagcaggc tttctacagc gaccctagcg tcctgtacat gtccctccac 
     5641 cgctacgacg atgggaactt cttcccaggc agcggggctc ctgatgaggt gggcacaggg 
     5701 cccggcgtgg gtttcaacgt caacatggct ttcaccggcg gcctggaccc ccccatggga 
     5761 gacgctgagt acttggcggc cttcagaacg gtggtcatgc cgatcgccag cgagtttgcc 
     5821 ccggatgtgg tgctggtgtc atcaggcttc gatgccgtgg agggccaccc cacccctctt 
     5881 gggggctaca acctctccgc cagatgcttc gggtacctga cgaagcagct gatgggcctg 
     5941 gctggcggcc ggattgtcct ggccctcgag ggaggccacg acctgaccgc catttgcgac 
     6001 gcctcggaag catgtgtttc tgccttgctg ggaaacgagc ttgatcctct cccagaaaag 
     6061 gttttacagc aaagacccaa tgcaaacgct gtccgttcca tggagaaagt catggagatc 
     6121 cacagcaagt actggcgctg cctgcagcgc acaacctcca cagcggggcg ttctctgatc 
     6181 gaggctcaga cttgcgagaa cgaagaagcc gagacggtca ccgccatggc ctcgctgtcc 
     6241 gtgggcgtga agcccgccga aaagagacca gatgaggagc ccatggaaga ggagccgccc 
     6301 ctgtaaggta ccaggtaagt gtacccaatt cgccctatag tgagtcgtat tacaattcac 
     6361 tcgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggagatcc aatttttaag 
     6421 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc acagtcccaa 
     6481 ggctcatttc aggcccctca gtcctcacag tctgttcatg atcataatca gccataccac 
     6541 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 
     6601 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 
     6661 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     6721 tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa 
     6781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 
     6841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 
     6901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 
     6961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 
     7021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 
     7081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 
     7141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 
     7201 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
     7261 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
     7321 aatattgaaa aaggaagaat cctgaggccg ggccataact tcgtataatg tatgctatac 
     7381 gaagttatcc atgggccccc cctcgacatg agtaaacttg gtctgacagt taccaatgct 
     7441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
     7501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
     7561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
     7621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
     7681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
     7741 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
     7801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
     7861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 
     7921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
     7981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
     8041 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
     8101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
     8161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
     8221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
     8281 gaatactcat actcttcctc aggttactca tatatacttt agattgattt aaaacttcat 
     8341 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 
     8401 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 
     8461 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 
     8521 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 
     8581 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 
     8641 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 
     8701 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 
     8761 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 
     8821 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 
     8881 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 
     8941 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 
     9001 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 
     9061 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 
     9121 ttatcccctg attctgtgga taaccgtatt accgcc 
//
