LOCUS pUCP20T-ecfp 5045 bp DNA circular SYN 25-NOV-2003 DEFINITION Cloning vector pUCP20T, complete sequence. ACCESSION U33750 VERSION U33750.1 GI:1144474 KEYWORDS . SOURCE Cloning vector pUCP20T (unknown) ORGANISM Cloning vector pUCP20T other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4174) AUTHORS West,S.E., Schweizer,H.P., Dall,C., Sample,A.K. and Runyen-Janecky,L.J. TITLE Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa JOURNAL Gene 148 (1), 81-86 (1994) PUBMED 7926843 REFERENCE 2 (bases 1 to 4174) AUTHORS Schweizer,H.P., Klassen,T. and Hoang,T. TITLE Improved methods for gene analysis and expression in Pseudomonas JOURNAL Unpublished REFERENCE 3 (bases 1 to 4174) AUTHORS Schweizer,H.P. and Klassen,T. TITLE Direct Submission JOURNAL Submitted (11-AUG-1995) Herbert P. Schweizer, Colorado State University, Microbiology, Fort Collins, CO 80523, USA FEATURES Location/Qualifiers promoter 131..159 /label=Pamp CDS 201..1061 /gene="bla" /codon_start=1 /transl_table=11 /product="beta lactamase" /protein_id="AAA86124.1" /db_xref="GI:1144477" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin complement(1216..1835) /label=pBR322 oriT complement(1885..2152) /note="oriT region" promoter 2420..2449 /label=Plac primer 2463..2486 /label="M13 R (-48)" primer 2487..2502 /label="M13 R (-24)" RBS 2522..2528 CDS 2556..3275 /gene="ecfp" /codon_start=1 /transl_table=11 /product="ecfp fluorescence protein" /protein_id="ABF74531.1" /db_xref="GI:104716872" /translation="MLSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK" gene 2556..3275 /gene="ecfp" terminator 3298..3392 /note="bacteriophage lambda T0" misc_feature complement(3382..>3404) /feature_type="Primer Region" /label="Reporter GCY R" primer complement(3439..3455) /label="M13 F (-20)" primer complement(3459..3482) /label="M13 F (-47)" primer complement(3459..3475) /label="M13 F (-40)" CDS complement(3616..4449) /gene="rep" /note="replication protein" /codon_start=1 /transl_table=11 /product="Rep" /protein_id="AAA86122.1" /db_xref="GI:1144475" /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDE VMYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWV QTIIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWD SIGQREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRK VTHLLFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDL A" rep_origin complement(4450..4859) /note="ori region from pRO1600" ORIGIN 1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 1861 GCTGGCCTTT TGCTCACATA AGCTAGCTTA TCGGCCAGCC TCGCAGAGCA GGATTCCCGT 1921 TGAGCACCGC CAGGTGCGAA TAAGGGACAG TGAAGAAGGA ACACCCGCTC GCGGGTGGGC 1981 CTACTTCACC TATCCTGCCC GGCTGACGCC GTTGGATACA CCAAGGAAAG TCTACACGAA 2041 CCCTTTGGCA AAATCCTGTA TATCGTGCGA AAAAGGATGG ATATACCGAA AAAATCGCTA 2101 TAATGACCCC GAAGCAGGGT TATGCAGCGG AAAGTATACC TTAAGGAATC CCCATGTTCT 2161 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA 2221 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC 2281 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 2341 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA 2401 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG 2461 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGGCGCGC 2521 CAAGAAGGTC TAGAATTAAA GAGGAGAAAT TAAGCATGCT GAGCAAGGGC GAGGAGCTGT 2581 TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA 2641 GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT 2701 GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTGGGGCG 2761 TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA 2821 TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA 2881 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA 2941 TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACATCAGCC 3001 ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC TTCAAGATCC 3061 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA 3121 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA 3181 GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG 3241 GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAATAAGC TTAATTAGCT GAGCTTGGAC 3301 TCCTGTTGAT AGATCCAGTA ATGACCTCAG AACTCCATCT GGATTTGTTC AGAACGCTCG 3361 GTTGCCGCCG GGCGTTTTTT ATTGGTGAGA ATCCAAGCTG GATCCTCTAG AGTCGACCTG 3421 CAGGCATGCA AGCTTGGCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG 3481 CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA 3541 AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAAAGGCA GGCCGGGCCG 3601 TGGTGGCCAC GGCCTCTAGG CCAGATCCAG CGGCATCTGG GTTAGTCGAG CGCGGGCCGC 3661 TTCCCATGTC TCACCAGGGC GAGCCTGTTT CGCGATCTCA GCATCTGAAA TCTTCCCGGC 3721 CTTGCGCTTC GCTGGGGCCT TACCCACCGC CTTGGCGGGC TTCTTCGGTC CAAAACTGAA 3781 CAACAGATGT GTGACCTTGC GCCCGGTCTT TCGCTGCGCC CACTCCACCT GTAGCGGGCT 3841 GTGCTCGTTG ATCTGCGTCA CGGCTGGATC AAGCACTCGC AACTTGAAGT CCTTGATCGA 3901 GGGATACCGG CCTTCCAGTT GAAACCACTT TCGCAGCTGG TCAATTTCTA TTTCGCGCTG 3961 GCCGATGCTG TCCCATTGCA TGAGCAGCTC GTAAAGCCTG ATCGCGTGGG TGCTGTCCAT 4021 CTTGGCCACG TCAGCCAAGG CGTATTTGGT GAACTGTTTG GTGAGTTCCG TCAGGTACGG 4081 CAGCATGTCT TTGGTGAACC TGAGTTCTAC ACGGCCCTCA CCCTCCCGGT AGATGATTGT 4141 TTGCACCCAG CCGGTAATCA TCACACTCGG TCTTTTCCCC TTGCCATTGG GCTCTTGGGT 4201 TAACCGGACT TCCCGCCGTT TCAGGCGCAG GGCCGCTTCT TTGAGCTGGT TGTAGGAAGA 4261 TTCGATAGGG ACACCCGCCA TCGTCGCTAT GTCCTCCGCC GTCACTGAAT ACATCACTTC 4321 ATCGGTGACA GGCTCGCTCC TCTTCACCTG GCTAATACAG GCCAGAACGA TCCGCTGTTC 4381 CTGAACACTG AGGCGATACG CGGCCTCGAC CAGGGCATTG CTTTTGTAAA CCATTGGGGG 4441 TGAGGCCACG TTCGACATTC CTTGTGTATA AGGGGACACT GTATCTGCGT CCCACAATAC 4501 AACAAATCCG TCCCTTTACA ACAACAAATC CGTCCCTTCT TAACAACAAA TCCGTCCCTT 4561 AATGGCAACA AATCCGTCCC TTTTTAAACT CTACAGGCCA CGGATTACGT GGCCTGTAGA 4621 CGTCCTAAAA GGTTTAAAAG GGAAAAGGAA GAAAAGGGTG GAAACGCAAA AAACGCACCA 4681 CTACGTGGCC CCGTTGGGGC CGCATTTGTG CCCCTGAAGG GGCGGGGGAG GCGTCTGGGC 4741 AATCCCCGTT TTACCAGTCC CCTATCGCCG CCTGAGAGGG CGCAGGAAGC GAGTAATCAG 4801 GGTATCGAGG CGGATTCACC CTTGGCGTCC AACCAGCGGC ACCAGCGGCG CCTGAGAGGT 4861 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC 4921 GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA 4981 AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG 5041 CGCGA //