LOCUS       pUCP20T-ecfp      5045 bp    DNA     circular SYN 25-NOV-2003
DEFINITION  Cloning vector pUCP20T, complete sequence.
ACCESSION   U33750
VERSION     U33750.1  GI:1144474
KEYWORDS    .
SOURCE      Cloning vector pUCP20T (unknown)
  ORGANISM  Cloning vector pUCP20T
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   West,S.E., Schweizer,H.P., Dall,C., Sample,A.K. and
            Runyen-Janecky,L.J.
  TITLE     Construction of improved Escherichia-Pseudomonas shuttle vectors
            derived from pUC18/19 and sequence of the region required for their
            replication in Pseudomonas aeruginosa
  JOURNAL   Gene 148 (1), 81-86 (1994)
   PUBMED   7926843
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Schweizer,H.P., Klassen,T. and Hoang,T.
  TITLE     Improved methods for gene analysis and expression in Pseudomonas
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4174)
  AUTHORS   Schweizer,H.P. and Klassen,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-1995) Herbert P. Schweizer, Colorado State
            University, Microbiology, Fort Collins, CO 80523, USA
FEATURES             Location/Qualifiers
     promoter        131..159
                     /label=Pamp
     CDS             201..1061
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta lactamase"
                     /protein_id="AAA86124.1"
                     /db_xref="GI:1144477"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     rep_origin      complement(1216..1835)
                     /label=pBR322
     oriT            complement(1885..2152)
                     /note="oriT region"
     promoter        2420..2449
                     /label=Plac
     primer          2463..2486
                     /label="M13 R (-48)"
     primer          2487..2502
                     /label="M13 R (-24)"
     RBS             2522..2528
     CDS             2556..3275
                     /gene="ecfp"
                     /codon_start=1
                     /transl_table=11
                     /product="ecfp fluorescence protein"
                     /protein_id="ABF74531.1"
                     /db_xref="GI:104716872"
                     /translation="MLSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     gene            2556..3275
                     /gene="ecfp"
     terminator      3298..3392
                     /note="bacteriophage lambda T0"
     misc_feature    complement(3382..>3404)
                     /feature_type="Primer Region"
                     /label="Reporter GCY R"
     primer          complement(3439..3455)
                     /label="M13 F (-20)"
     primer          complement(3459..3482)
                     /label="M13 F (-47)"
     primer          complement(3459..3475)
                     /label="M13 F (-40)"
     CDS             complement(3616..4449)
                     /gene="rep"
                     /note="replication protein"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAA86122.1"
                     /db_xref="GI:1144475"
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDE
                     VMYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWV
                     QTIIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWD
                     SIGQREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRK
                     VTHLLFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDL
                     A"
     rep_origin      complement(4450..4859)
                     /note="ori region from pRO1600"
ORIGIN
        1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
       61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT
      121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
      181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
      241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
      301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA
      361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC
      421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC
      481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
      541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA
      601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG
      661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG
      721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG
      781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
      841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG
      901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT
      961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC
     1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT
     1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA
     1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
     1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
     1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
     1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC
     1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
     1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
     1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
     1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
     1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
     1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
     1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
     1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
     1861 GCTGGCCTTT TGCTCACATA AGCTAGCTTA TCGGCCAGCC TCGCAGAGCA GGATTCCCGT
     1921 TGAGCACCGC CAGGTGCGAA TAAGGGACAG TGAAGAAGGA ACACCCGCTC GCGGGTGGGC
     1981 CTACTTCACC TATCCTGCCC GGCTGACGCC GTTGGATACA CCAAGGAAAG TCTACACGAA
     2041 CCCTTTGGCA AAATCCTGTA TATCGTGCGA AAAAGGATGG ATATACCGAA AAAATCGCTA
     2101 TAATGACCCC GAAGCAGGGT TATGCAGCGG AAAGTATACC TTAAGGAATC CCCATGTTCT
     2161 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA
     2221 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC
     2281 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG
     2341 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA
     2401 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG
     2461 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGGCGCGC
     2521 CAAGAAGGTC TAGAATTAAA GAGGAGAAAT TAAGCATGCT GAGCAAGGGC GAGGAGCTGT
     2581 TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA
     2641 GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT
     2701 GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTGGGGCG
     2761 TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA
     2821 TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA
     2881 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA
     2941 TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACATCAGCC
     3001 ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC TTCAAGATCC
     3061 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA
     3121 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA
     3181 GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG
     3241 GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAATAAGC TTAATTAGCT GAGCTTGGAC
     3301 TCCTGTTGAT AGATCCAGTA ATGACCTCAG AACTCCATCT GGATTTGTTC AGAACGCTCG
     3361 GTTGCCGCCG GGCGTTTTTT ATTGGTGAGA ATCCAAGCTG GATCCTCTAG AGTCGACCTG
     3421 CAGGCATGCA AGCTTGGCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG
     3481 CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA
     3541 AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAAAGGCA GGCCGGGCCG
     3601 TGGTGGCCAC GGCCTCTAGG CCAGATCCAG CGGCATCTGG GTTAGTCGAG CGCGGGCCGC
     3661 TTCCCATGTC TCACCAGGGC GAGCCTGTTT CGCGATCTCA GCATCTGAAA TCTTCCCGGC
     3721 CTTGCGCTTC GCTGGGGCCT TACCCACCGC CTTGGCGGGC TTCTTCGGTC CAAAACTGAA
     3781 CAACAGATGT GTGACCTTGC GCCCGGTCTT TCGCTGCGCC CACTCCACCT GTAGCGGGCT
     3841 GTGCTCGTTG ATCTGCGTCA CGGCTGGATC AAGCACTCGC AACTTGAAGT CCTTGATCGA
     3901 GGGATACCGG CCTTCCAGTT GAAACCACTT TCGCAGCTGG TCAATTTCTA TTTCGCGCTG
     3961 GCCGATGCTG TCCCATTGCA TGAGCAGCTC GTAAAGCCTG ATCGCGTGGG TGCTGTCCAT
     4021 CTTGGCCACG TCAGCCAAGG CGTATTTGGT GAACTGTTTG GTGAGTTCCG TCAGGTACGG
     4081 CAGCATGTCT TTGGTGAACC TGAGTTCTAC ACGGCCCTCA CCCTCCCGGT AGATGATTGT
     4141 TTGCACCCAG CCGGTAATCA TCACACTCGG TCTTTTCCCC TTGCCATTGG GCTCTTGGGT
     4201 TAACCGGACT TCCCGCCGTT TCAGGCGCAG GGCCGCTTCT TTGAGCTGGT TGTAGGAAGA
     4261 TTCGATAGGG ACACCCGCCA TCGTCGCTAT GTCCTCCGCC GTCACTGAAT ACATCACTTC
     4321 ATCGGTGACA GGCTCGCTCC TCTTCACCTG GCTAATACAG GCCAGAACGA TCCGCTGTTC
     4381 CTGAACACTG AGGCGATACG CGGCCTCGAC CAGGGCATTG CTTTTGTAAA CCATTGGGGG
     4441 TGAGGCCACG TTCGACATTC CTTGTGTATA AGGGGACACT GTATCTGCGT CCCACAATAC
     4501 AACAAATCCG TCCCTTTACA ACAACAAATC CGTCCCTTCT TAACAACAAA TCCGTCCCTT
     4561 AATGGCAACA AATCCGTCCC TTTTTAAACT CTACAGGCCA CGGATTACGT GGCCTGTAGA
     4621 CGTCCTAAAA GGTTTAAAAG GGAAAAGGAA GAAAAGGGTG GAAACGCAAA AAACGCACCA
     4681 CTACGTGGCC CCGTTGGGGC CGCATTTGTG CCCCTGAAGG GGCGGGGGAG GCGTCTGGGC
     4741 AATCCCCGTT TTACCAGTCC CCTATCGCCG CCTGAGAGGG CGCAGGAAGC GAGTAATCAG
     4801 GGTATCGAGG CGGATTCACC CTTGGCGTCC AACCAGCGGC ACCAGCGGCG CCTGAGAGGT
     4861 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC
     4921 GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA
     4981 AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG
     5041 CGCGA
//
