LOCUS pUCP20T-tdTomato 5605 bp DNA circular SYN 25-NOV-2003 DEFINITION Cloning vector pUCP20T, complete sequence. ACCESSION U33750 VERSION U33750.1 GI:1144474 KEYWORDS . SOURCE Cloning vector pUCP20T (unknown) ORGANISM Cloning vector pUCP20T other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4174) AUTHORS West,S.E., Schweizer,H.P., Dall,C., Sample,A.K. and Runyen-Janecky,L.J. TITLE Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa JOURNAL Gene 148 (1), 81-86 (1994) PUBMED 7926843 REFERENCE 2 (bases 1 to 4174) AUTHORS Schweizer,H.P., Klassen,T. and Hoang,T. TITLE Improved methods for gene analysis and expression in Pseudomonas JOURNAL Unpublished REFERENCE 3 (bases 1 to 4174) AUTHORS Schweizer,H.P. and Klassen,T. TITLE Direct Submission JOURNAL Submitted (11-AUG-1995) Herbert P. Schweizer, Colorado State University, Microbiology, Fort Collins, CO 80523, USA FEATURES Location/Qualifiers promoter 131..159 /label=Pamp CDS 201..1061 /gene="bla" /codon_start=1 /transl_table=11 /product="beta lactamase" /protein_id="AAA86124.1" /db_xref="GI:1144477" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin complement(1216..1835) /label=pBR322 oriT complement(1885..2152) /note="oriT region" promoter 2420..2449 /label=Plac primer 2463..2486 /label="M13 R (-48)" primer 2487..2502 /label="M13 R (-24)" RBS 2522..2528 CDS 2529..3959 /cds_type=ORF /label=tdTomato gene 2529..3959 /label=tdTomato primer complement(3999..4015) /label="M13 F (-20)" primer complement(4019..4042) /label="M13 F (-47)" primer complement(4019..4035) /label="M13 F (-40)" CDS complement(4176..5009) /gene="rep" /note="replication protein" /codon_start=1 /transl_table=11 /product="Rep" /protein_id="AAA86122.1" /db_xref="GI:1144475" /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDE VMYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWV QTIIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWD SIGQREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRK VTHLLFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDL A" rep_origin complement(5010..5419) /note="ori region from pRO1600" ORIGIN 1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 1861 GCTGGCCTTT TGCTCACATA AGCTAGCTTA TCGGCCAGCC TCGCAGAGCA GGATTCCCGT 1921 TGAGCACCGC CAGGTGCGAA TAAGGGACAG TGAAGAAGGA ACACCCGCTC GCGGGTGGGC 1981 CTACTTCACC TATCCTGCCC GGCTGACGCC GTTGGATACA CCAAGGAAAG TCTACACGAA 2041 CCCTTTGGCA AAATCCTGTA TATCGTGCGA AAAAGGATGG ATATACCGAA AAAATCGCTA 2101 TAATGACCCC GAAGCAGGGT TATGCAGCGG AAAGTATACC TTAAGGAATC CCCATGTTCT 2161 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA 2221 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC 2281 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 2341 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA 2401 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG 2461 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGGCGCGC 2521 CAAGAAGGat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc 2581 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 2641 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg 2701 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg 2761 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 2821 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca 2881 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc 2941 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc 3001 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt 3061 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca 3121 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct 3181 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct 3241 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca 3301 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg 3361 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc 3421 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc 3481 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc 3541 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg 3601 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg 3661 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg 3721 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg 3781 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg 3841 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg 3901 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtaaG 3961 GATCCTCTAG AGTCGACCTG CAGGCATGCA AGCTTGGCAC TGGCCGTCGT TTTACAACGT 4021 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC 4081 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC 4141 CTGAAAGGCA GGCCGGGCCG TGGTGGCCAC GGCCTCTAGG CCAGATCCAG CGGCATCTGG 4201 GTTAGTCGAG CGCGGGCCGC TTCCCATGTC TCACCAGGGC GAGCCTGTTT CGCGATCTCA 4261 GCATCTGAAA TCTTCCCGGC CTTGCGCTTC GCTGGGGCCT TACCCACCGC CTTGGCGGGC 4321 TTCTTCGGTC CAAAACTGAA CAACAGATGT GTGACCTTGC GCCCGGTCTT TCGCTGCGCC 4381 CACTCCACCT GTAGCGGGCT GTGCTCGTTG ATCTGCGTCA CGGCTGGATC AAGCACTCGC 4441 AACTTGAAGT CCTTGATCGA GGGATACCGG CCTTCCAGTT GAAACCACTT TCGCAGCTGG 4501 TCAATTTCTA TTTCGCGCTG GCCGATGCTG TCCCATTGCA TGAGCAGCTC GTAAAGCCTG 4561 ATCGCGTGGG TGCTGTCCAT CTTGGCCACG TCAGCCAAGG CGTATTTGGT GAACTGTTTG 4621 GTGAGTTCCG TCAGGTACGG CAGCATGTCT TTGGTGAACC TGAGTTCTAC ACGGCCCTCA 4681 CCCTCCCGGT AGATGATTGT TTGCACCCAG CCGGTAATCA TCACACTCGG TCTTTTCCCC 4741 TTGCCATTGG GCTCTTGGGT TAACCGGACT TCCCGCCGTT TCAGGCGCAG GGCCGCTTCT 4801 TTGAGCTGGT TGTAGGAAGA TTCGATAGGG ACACCCGCCA TCGTCGCTAT GTCCTCCGCC 4861 GTCACTGAAT ACATCACTTC ATCGGTGACA GGCTCGCTCC TCTTCACCTG GCTAATACAG 4921 GCCAGAACGA TCCGCTGTTC CTGAACACTG AGGCGATACG CGGCCTCGAC CAGGGCATTG 4981 CTTTTGTAAA CCATTGGGGG TGAGGCCACG TTCGACATTC CTTGTGTATA AGGGGACACT 5041 GTATCTGCGT CCCACAATAC AACAAATCCG TCCCTTTACA ACAACAAATC CGTCCCTTCT 5101 TAACAACAAA TCCGTCCCTT AATGGCAACA AATCCGTCCC TTTTTAAACT CTACAGGCCA 5161 CGGATTACGT GGCCTGTAGA CGTCCTAAAA GGTTTAAAAG GGAAAAGGAA GAAAAGGGTG 5221 GAAACGCAAA AAACGCACCA CTACGTGGCC CCGTTGGGGC CGCATTTGTG CCCCTGAAGG 5281 GGCGGGGGAG GCGTCTGGGC AATCCCCGTT TTACCAGTCC CCTATCGCCG CCTGAGAGGG 5341 CGCAGGAAGC GAGTAATCAG GGTATCGAGG CGGATTCACC CTTGGCGTCC AACCAGCGGC 5401 ACCAGCGGCG CCTGAGAGGT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG 5461 TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC 5521 CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT 5581 TCACCGTCAT CACCGAAACG CGCGA //