LOCUS       pUCP20T-tdTomato      5605 bp    DNA     circular SYN 25-NOV-2003
DEFINITION  Cloning vector pUCP20T, complete sequence.
ACCESSION   U33750
VERSION     U33750.1  GI:1144474
KEYWORDS    .
SOURCE      Cloning vector pUCP20T (unknown)
  ORGANISM  Cloning vector pUCP20T
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   West,S.E., Schweizer,H.P., Dall,C., Sample,A.K. and
            Runyen-Janecky,L.J.
  TITLE     Construction of improved Escherichia-Pseudomonas shuttle vectors
            derived from pUC18/19 and sequence of the region required for their
            replication in Pseudomonas aeruginosa
  JOURNAL   Gene 148 (1), 81-86 (1994)
   PUBMED   7926843
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Schweizer,H.P., Klassen,T. and Hoang,T.
  TITLE     Improved methods for gene analysis and expression in Pseudomonas
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4174)
  AUTHORS   Schweizer,H.P. and Klassen,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-1995) Herbert P. Schweizer, Colorado State
            University, Microbiology, Fort Collins, CO 80523, USA
FEATURES             Location/Qualifiers
     promoter        131..159
                     /label=Pamp
     CDS             201..1061
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta lactamase"
                     /protein_id="AAA86124.1"
                     /db_xref="GI:1144477"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     rep_origin      complement(1216..1835)
                     /label=pBR322
     oriT            complement(1885..2152)
                     /note="oriT region"
     promoter        2420..2449
                     /label=Plac
     primer          2463..2486
                     /label="M13 R (-48)"
     primer          2487..2502
                     /label="M13 R (-24)"
     RBS             2522..2528
     CDS             2529..3959
                     /cds_type=ORF
                     /label=tdTomato
     gene            2529..3959
                     /label=tdTomato
     primer          complement(3999..4015)
                     /label="M13 F (-20)"
     primer          complement(4019..4042)
                     /label="M13 F (-47)"
     primer          complement(4019..4035)
                     /label="M13 F (-40)"
     CDS             complement(4176..5009)
                     /gene="rep"
                     /note="replication protein"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAA86122.1"
                     /db_xref="GI:1144475"
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDE
                     VMYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWV
                     QTIIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWD
                     SIGQREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRK
                     VTHLLFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDL
                     A"
     rep_origin      complement(5010..5419)
                     /note="ori region from pRO1600"
ORIGIN
        1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
       61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT
      121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
      181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
      241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
      301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA
      361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC
      421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC
      481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
      541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA
      601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG
      661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG
      721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG
      781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
      841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG
      901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT
      961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC
     1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT
     1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA
     1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
     1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
     1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
     1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC
     1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
     1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
     1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
     1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
     1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
     1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
     1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
     1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
     1861 GCTGGCCTTT TGCTCACATA AGCTAGCTTA TCGGCCAGCC TCGCAGAGCA GGATTCCCGT
     1921 TGAGCACCGC CAGGTGCGAA TAAGGGACAG TGAAGAAGGA ACACCCGCTC GCGGGTGGGC
     1981 CTACTTCACC TATCCTGCCC GGCTGACGCC GTTGGATACA CCAAGGAAAG TCTACACGAA
     2041 CCCTTTGGCA AAATCCTGTA TATCGTGCGA AAAAGGATGG ATATACCGAA AAAATCGCTA
     2101 TAATGACCCC GAAGCAGGGT TATGCAGCGG AAAGTATACC TTAAGGAATC CCCATGTTCT
     2161 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA
     2221 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC
     2281 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG
     2341 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA
     2401 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG
     2461 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGGCGCGC
     2521 CAAGAAGGat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     2581 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     2641 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     2701 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     2761 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     2821 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     2881 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     2941 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     3001 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     3061 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     3121 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     3181 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     3241 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca
     3301 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     3361 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     3421 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     3481 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     3541 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     3601 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     3661 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     3721 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     3781 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     3841 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     3901 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtaaG
     3961 GATCCTCTAG AGTCGACCTG CAGGCATGCA AGCTTGGCAC TGGCCGTCGT TTTACAACGT
     4021 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC
     4081 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC
     4141 CTGAAAGGCA GGCCGGGCCG TGGTGGCCAC GGCCTCTAGG CCAGATCCAG CGGCATCTGG
     4201 GTTAGTCGAG CGCGGGCCGC TTCCCATGTC TCACCAGGGC GAGCCTGTTT CGCGATCTCA
     4261 GCATCTGAAA TCTTCCCGGC CTTGCGCTTC GCTGGGGCCT TACCCACCGC CTTGGCGGGC
     4321 TTCTTCGGTC CAAAACTGAA CAACAGATGT GTGACCTTGC GCCCGGTCTT TCGCTGCGCC
     4381 CACTCCACCT GTAGCGGGCT GTGCTCGTTG ATCTGCGTCA CGGCTGGATC AAGCACTCGC
     4441 AACTTGAAGT CCTTGATCGA GGGATACCGG CCTTCCAGTT GAAACCACTT TCGCAGCTGG
     4501 TCAATTTCTA TTTCGCGCTG GCCGATGCTG TCCCATTGCA TGAGCAGCTC GTAAAGCCTG
     4561 ATCGCGTGGG TGCTGTCCAT CTTGGCCACG TCAGCCAAGG CGTATTTGGT GAACTGTTTG
     4621 GTGAGTTCCG TCAGGTACGG CAGCATGTCT TTGGTGAACC TGAGTTCTAC ACGGCCCTCA
     4681 CCCTCCCGGT AGATGATTGT TTGCACCCAG CCGGTAATCA TCACACTCGG TCTTTTCCCC
     4741 TTGCCATTGG GCTCTTGGGT TAACCGGACT TCCCGCCGTT TCAGGCGCAG GGCCGCTTCT
     4801 TTGAGCTGGT TGTAGGAAGA TTCGATAGGG ACACCCGCCA TCGTCGCTAT GTCCTCCGCC
     4861 GTCACTGAAT ACATCACTTC ATCGGTGACA GGCTCGCTCC TCTTCACCTG GCTAATACAG
     4921 GCCAGAACGA TCCGCTGTTC CTGAACACTG AGGCGATACG CGGCCTCGAC CAGGGCATTG
     4981 CTTTTGTAAA CCATTGGGGG TGAGGCCACG TTCGACATTC CTTGTGTATA AGGGGACACT
     5041 GTATCTGCGT CCCACAATAC AACAAATCCG TCCCTTTACA ACAACAAATC CGTCCCTTCT
     5101 TAACAACAAA TCCGTCCCTT AATGGCAACA AATCCGTCCC TTTTTAAACT CTACAGGCCA
     5161 CGGATTACGT GGCCTGTAGA CGTCCTAAAA GGTTTAAAAG GGAAAAGGAA GAAAAGGGTG
     5221 GAAACGCAAA AAACGCACCA CTACGTGGCC CCGTTGGGGC CGCATTTGTG CCCCTGAAGG
     5281 GGCGGGGGAG GCGTCTGGGC AATCCCCGTT TTACCAGTCC CCTATCGCCG CCTGAGAGGG
     5341 CGCAGGAAGC GAGTAATCAG GGTATCGAGG CGGATTCACC CTTGGCGTCC AACCAGCGGC
     5401 ACCAGCGGCG CCTGAGAGGT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG
     5461 TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC
     5521 CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT
     5581 TCACCGTCAT CACCGAAACG CGCGA
//
