LOCUS pUCP20T-mPlum 4854 bp DNA circular SYN 25-NOV-2003 DEFINITION Cloning vector pUCP20T, complete sequence. ACCESSION U33750 VERSION U33750.1 GI:1144474 KEYWORDS . SOURCE Cloning vector pUCP20T (unknown) ORGANISM Cloning vector pUCP20T other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4174) AUTHORS West,S.E., Schweizer,H.P., Dall,C., Sample,A.K. and Runyen-Janecky,L.J. TITLE Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa JOURNAL Gene 148 (1), 81-86 (1994) PUBMED 7926843 REFERENCE 2 (bases 1 to 4174) AUTHORS Schweizer,H.P., Klassen,T. and Hoang,T. TITLE Improved methods for gene analysis and expression in Pseudomonas JOURNAL Unpublished REFERENCE 3 (bases 1 to 4174) AUTHORS Schweizer,H.P. and Klassen,T. TITLE Direct Submission JOURNAL Submitted (11-AUG-1995) Herbert P. Schweizer, Colorado State University, Microbiology, Fort Collins, CO 80523, USA FEATURES Location/Qualifiers promoter 131..159 /label=Pamp CDS 201..1061 /gene="bla" /codon_start=1 /transl_table=11 /product="beta lactamase" /protein_id="AAA86124.1" /db_xref="GI:1144477" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin complement(1216..1835) /label=pBR322 oriT complement(1885..2152) /note="oriT region" promoter 2420..2449 /label=Plac primer 2463..2486 /label="M13 R (-48)" primer 2487..2501 /label="M13 R (-24)" RBS 2521..2527 CDS 2528..3208 /label="ORF frame 2" /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQT ARLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED GGVVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG EMKMRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERA EGRHSTGA*" /label=mPlum gene 2531..3205 /label="mPlum" /gene="mPlum" /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQT ARLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED GGVVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG EMKMRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERA EGRHSTGA*" misc_feature 3107..3133 /label="I197 (mTangerine)" /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQT ARLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED GGVVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG EMKMRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERA EGRHSTGA*" misc_feature 3123..3146 /label="dsRed1_C_primer" /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQT ARLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED GGVVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG EMKMRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERA EGRHSTGA*" primer complement(3248..3264) /label="M13 F (-20)" primer complement(3268..3291) /label="M13 F (-47)" primer complement(3268..3284) /label="M13 F (-40)" CDS complement(3425..4258) /gene="rep" /note="replication protein" /codon_start=1 /transl_table=11 /product="Rep" /protein_id="AAA86122.1" /db_xref="GI:1144475" /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDE VMYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWV QTIIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWD SIGQREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRK VTHLLFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDL A" rep_origin complement(4259..4668) /note="ori region from pRO1600" ORIGIN 1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 1861 GCTGGCCTTT TGCTCACATA AGCTAGCTTA TCGGCCAGCC TCGCAGAGCA GGATTCCCGT 1921 TGAGCACCGC CAGGTGCGAA TAAGGGACAG TGAAGAAGGA ACACCCGCTC GCGGGTGGGC 1981 CTACTTCACC TATCCTGCCC GGCTGACGCC GTTGGATACA CCAAGGAAAG TCTACACGAA 2041 CCCTTTGGCA AAATCCTGTA TATCGTGCGA AAAAGGATGG ATATACCGAA AAAATCGCTA 2101 TAATGACCCC GAAGCAGGGT TATGCAGCGG AAAGTATACC TTAAGGAATC CCCATGTTCT 2161 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA 2221 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC 2281 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 2341 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA 2401 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG 2461 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACAT GATTACGAAT TCGGCGCGCC 2521 AAGAAGGATG GTGAGCAAGG GCGAGGAGGT CATCAAGGAG TTCATGCGCT TCAAGGAGCA 2581 CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC 2641 CTATGAGGGC ACCCAGACCG CCAGGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC 2701 CTGGGACATC CTGTCCCCTC AGATCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC 2761 CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT 2821 GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA 2881 GTTCATCTAC AAGGTGAAGG TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA 2941 GAAGAAGACC ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT 3001 GAAGGGCGAG ATGAAGATGA GGCTGAGGCT GAAGGACGGC GGCCACTACG ACGCCGAGGT 3061 CAAGACCACC TACATGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA AGACCGACAT 3121 CAAGCTGGAC ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAGCGCGC 3181 CGAGGGCCGC CACTCCACCG GCGCCTAAGG ATCCTCTAGA GTCGACCTGC AGGCATGCAA 3241 GCTTGGCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC 3301 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA 3361 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAAAGGCAG GCCGGGCCGT GGTGGCCACG 3421 GCCTCTAGGC CAGATCCAGC GGCATCTGGG TTAGTCGAGC GCGGGCCGCT TCCCATGTCT 3481 CACCAGGGCG AGCCTGTTTC GCGATCTCAG CATCTGAAAT CTTCCCGGCC TTGCGCTTCG 3541 CTGGGGCCTT ACCCACCGCC TTGGCGGGCT TCTTCGGTCC AAAACTGAAC AACAGATGTG 3601 TGACCTTGCG CCCGGTCTTT CGCTGCGCCC ACTCCACCTG TAGCGGGCTG TGCTCGTTGA 3661 TCTGCGTCAC GGCTGGATCA AGCACTCGCA ACTTGAAGTC CTTGATCGAG GGATACCGGC 3721 CTTCCAGTTG AAACCACTTT CGCAGCTGGT CAATTTCTAT TTCGCGCTGG CCGATGCTGT 3781 CCCATTGCAT GAGCAGCTCG TAAAGCCTGA TCGCGTGGGT GCTGTCCATC TTGGCCACGT 3841 CAGCCAAGGC GTATTTGGTG AACTGTTTGG TGAGTTCCGT CAGGTACGGC AGCATGTCTT 3901 TGGTGAACCT GAGTTCTACA CGGCCCTCAC CCTCCCGGTA GATGATTGTT TGCACCCAGC 3961 CGGTAATCAT CACACTCGGT CTTTTCCCCT TGCCATTGGG CTCTTGGGTT AACCGGACTT 4021 CCCGCCGTTT CAGGCGCAGG GCCGCTTCTT TGAGCTGGTT GTAGGAAGAT TCGATAGGGA 4081 CACCCGCCAT CGTCGCTATG TCCTCCGCCG TCACTGAATA CATCACTTCA TCGGTGACAG 4141 GCTCGCTCCT CTTCACCTGG CTAATACAGG CCAGAACGAT CCGCTGTTCC TGAACACTGA 4201 GGCGATACGC GGCCTCGACC AGGGCATTGC TTTTGTAAAC CATTGGGGGT GAGGCCACGT 4261 TCGACATTCC TTGTGTATAA GGGGACACTG TATCTGCGTC CCACAATACA ACAAATCCGT 4321 CCCTTTACAA CAACAAATCC GTCCCTTCTT AACAACAAAT CCGTCCCTTA ATGGCAACAA 4381 ATCCGTCCCT TTTTAAACTC TACAGGCCAC GGATTACGTG GCCTGTAGAC GTCCTAAAAG 4441 GTTTAAAAGG GAAAAGGAAG AAAAGGGTGG AAACGCAAAA AACGCACCAC TACGTGGCCC 4501 CGTTGGGGCC GCATTTGTGC CCCTGAAGGG GCGGGGGAGG CGTCTGGGCA ATCCCCGTTT 4561 TACCAGTCCC CTATCGCCGC CTGAGAGGGC GCAGGAAGCG AGTAATCAGG GTATCGAGGC 4621 GGATTCACCC TTGGCGTCCA ACCAGCGGCA CCAGCGGCGC CTGAGAGGTA TGGTGCACTC 4681 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG 4741 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG 4801 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGA //