LOCUS       pUCP20T-mPlum      4854 bp    DNA     circular SYN 25-NOV-2003
DEFINITION  Cloning vector pUCP20T, complete sequence.
ACCESSION   U33750
VERSION     U33750.1  GI:1144474
KEYWORDS    .
SOURCE      Cloning vector pUCP20T (unknown)
  ORGANISM  Cloning vector pUCP20T
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   West,S.E., Schweizer,H.P., Dall,C., Sample,A.K. and
            Runyen-Janecky,L.J.
  TITLE     Construction of improved Escherichia-Pseudomonas shuttle vectors
            derived from pUC18/19 and sequence of the region required for their
            replication in Pseudomonas aeruginosa
  JOURNAL   Gene 148 (1), 81-86 (1994)
   PUBMED   7926843
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Schweizer,H.P., Klassen,T. and Hoang,T.
  TITLE     Improved methods for gene analysis and expression in Pseudomonas
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4174)
  AUTHORS   Schweizer,H.P. and Klassen,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-1995) Herbert P. Schweizer, Colorado State
            University, Microbiology, Fort Collins, CO 80523, USA
FEATURES             Location/Qualifiers
     promoter        131..159
                     /label=Pamp
     CDS             201..1061
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta lactamase"
                     /protein_id="AAA86124.1"
                     /db_xref="GI:1144477"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     rep_origin      complement(1216..1835)
                     /label=pBR322
     oriT            complement(1885..2152)
                     /note="oriT region"
     promoter        2420..2449
                     /label=Plac
     primer          2463..2486
                     /label="M13 R (-48)"
     primer          2487..2501
                     /label="M13 R (-24)"
     RBS             2521..2527
     CDS             2528..3208
                     /label="ORF frame 2"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQT
                     ARLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EMKMRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGA*"
                     /label=mPlum
     gene            2531..3205
                     /label="mPlum"
                     /gene="mPlum"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQT
                     ARLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EMKMRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGA*"
     misc_feature    3107..3133
                     /label="I197 (mTangerine)"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQT
                     ARLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EMKMRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGA*"
     misc_feature    3123..3146
                     /label="dsRed1_C_primer"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQT
                     ARLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EMKMRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGA*"
     primer          complement(3248..3264)
                     /label="M13 F (-20)"
     primer          complement(3268..3291)
                     /label="M13 F (-47)"
     primer          complement(3268..3284)
                     /label="M13 F (-40)"
     CDS             complement(3425..4258)
                     /gene="rep"
                     /note="replication protein"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAA86122.1"
                     /db_xref="GI:1144475"
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDE
                     VMYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWV
                     QTIIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWD
                     SIGQREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRK
                     VTHLLFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDL
                     A"
     rep_origin      complement(4259..4668)
                     /note="ori region from pRO1600"
ORIGIN
        1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
       61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT
      121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
      181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
      241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
      301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA
      361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC
      421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC
      481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
      541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA
      601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG
      661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG
      721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG
      781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
      841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG
      901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT
      961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC
     1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT
     1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA
     1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
     1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
     1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
     1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC
     1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
     1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
     1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
     1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
     1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
     1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
     1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
     1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
     1861 GCTGGCCTTT TGCTCACATA AGCTAGCTTA TCGGCCAGCC TCGCAGAGCA GGATTCCCGT
     1921 TGAGCACCGC CAGGTGCGAA TAAGGGACAG TGAAGAAGGA ACACCCGCTC GCGGGTGGGC
     1981 CTACTTCACC TATCCTGCCC GGCTGACGCC GTTGGATACA CCAAGGAAAG TCTACACGAA
     2041 CCCTTTGGCA AAATCCTGTA TATCGTGCGA AAAAGGATGG ATATACCGAA AAAATCGCTA
     2101 TAATGACCCC GAAGCAGGGT TATGCAGCGG AAAGTATACC TTAAGGAATC CCCATGTTCT
     2161 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA
     2221 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC
     2281 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG
     2341 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA
     2401 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG
     2461 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACAT GATTACGAAT TCGGCGCGCC
     2521 AAGAAGGATG GTGAGCAAGG GCGAGGAGGT CATCAAGGAG TTCATGCGCT TCAAGGAGCA
     2581 CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC
     2641 CTATGAGGGC ACCCAGACCG CCAGGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC
     2701 CTGGGACATC CTGTCCCCTC AGATCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC
     2761 CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT
     2821 GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA
     2881 GTTCATCTAC AAGGTGAAGG TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA
     2941 GAAGAAGACC ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT
     3001 GAAGGGCGAG ATGAAGATGA GGCTGAGGCT GAAGGACGGC GGCCACTACG ACGCCGAGGT
     3061 CAAGACCACC TACATGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA AGACCGACAT
     3121 CAAGCTGGAC ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAGCGCGC
     3181 CGAGGGCCGC CACTCCACCG GCGCCTAAGG ATCCTCTAGA GTCGACCTGC AGGCATGCAA
     3241 GCTTGGCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC
     3301 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA
     3361 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAAAGGCAG GCCGGGCCGT GGTGGCCACG
     3421 GCCTCTAGGC CAGATCCAGC GGCATCTGGG TTAGTCGAGC GCGGGCCGCT TCCCATGTCT
     3481 CACCAGGGCG AGCCTGTTTC GCGATCTCAG CATCTGAAAT CTTCCCGGCC TTGCGCTTCG
     3541 CTGGGGCCTT ACCCACCGCC TTGGCGGGCT TCTTCGGTCC AAAACTGAAC AACAGATGTG
     3601 TGACCTTGCG CCCGGTCTTT CGCTGCGCCC ACTCCACCTG TAGCGGGCTG TGCTCGTTGA
     3661 TCTGCGTCAC GGCTGGATCA AGCACTCGCA ACTTGAAGTC CTTGATCGAG GGATACCGGC
     3721 CTTCCAGTTG AAACCACTTT CGCAGCTGGT CAATTTCTAT TTCGCGCTGG CCGATGCTGT
     3781 CCCATTGCAT GAGCAGCTCG TAAAGCCTGA TCGCGTGGGT GCTGTCCATC TTGGCCACGT
     3841 CAGCCAAGGC GTATTTGGTG AACTGTTTGG TGAGTTCCGT CAGGTACGGC AGCATGTCTT
     3901 TGGTGAACCT GAGTTCTACA CGGCCCTCAC CCTCCCGGTA GATGATTGTT TGCACCCAGC
     3961 CGGTAATCAT CACACTCGGT CTTTTCCCCT TGCCATTGGG CTCTTGGGTT AACCGGACTT
     4021 CCCGCCGTTT CAGGCGCAGG GCCGCTTCTT TGAGCTGGTT GTAGGAAGAT TCGATAGGGA
     4081 CACCCGCCAT CGTCGCTATG TCCTCCGCCG TCACTGAATA CATCACTTCA TCGGTGACAG
     4141 GCTCGCTCCT CTTCACCTGG CTAATACAGG CCAGAACGAT CCGCTGTTCC TGAACACTGA
     4201 GGCGATACGC GGCCTCGACC AGGGCATTGC TTTTGTAAAC CATTGGGGGT GAGGCCACGT
     4261 TCGACATTCC TTGTGTATAA GGGGACACTG TATCTGCGTC CCACAATACA ACAAATCCGT
     4321 CCCTTTACAA CAACAAATCC GTCCCTTCTT AACAACAAAT CCGTCCCTTA ATGGCAACAA
     4381 ATCCGTCCCT TTTTAAACTC TACAGGCCAC GGATTACGTG GCCTGTAGAC GTCCTAAAAG
     4441 GTTTAAAAGG GAAAAGGAAG AAAAGGGTGG AAACGCAAAA AACGCACCAC TACGTGGCCC
     4501 CGTTGGGGCC GCATTTGTGC CCCTGAAGGG GCGGGGGAGG CGTCTGGGCA ATCCCCGTTT
     4561 TACCAGTCCC CTATCGCCGC CTGAGAGGGC GCAGGAAGCG AGTAATCAGG GTATCGAGGC
     4621 GGATTCACCC TTGGCGTCCA ACCAGCGGCA CCAGCGGCGC CTGAGAGGTA TGGTGCACTC
     4681 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG
     4741 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG
     4801 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGA
//
